• 제목/요약/키워드: and oxygen

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공배양 및 산소농도가 한우 난포란의 체외발생에 미치는 영향 (The Effect of Co-culture and Oxygen Concentration on In Virto Fertilization of Follicular Oocytes in Korean Native Cattle)

  • 이재관;윤준진;황성수;윤종택;김창근;정영채
    • 한국가축번식학회지
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    • 제22권1호
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    • pp.43-50
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    • 1998
  • The effect of oxygen tension on embryonic development in co-culture was evaluated from the standpoint of the reduction of dissolved oxygen concentration by the oxygen consumption of feeder cells. Three co-culture systems using bovine oviductal epitherial cells (BOEC), African green monkey kidney cells (Vero cells) or buffalo rat liver cells (BRLC) have been compared in terms of development of bovine embryos derived from oocytes matured and fertilized in vitro. Among the co-cultured embryo, Vero cells su, pp.rted the highest developmental rate (29%) and the other two showed the similar rates. When the co-cultures were incubated in three different oxygen tension such as 5, 10, 20% oxygen atmosphere, embryos co-cultured with Vero cells at 10%-O2 resulted in the highest percentage of development. From the measurement of oxygen consumption of feeder cells, BRLC consumed 1.38 10-10 mg-O2/min/cell which was higher than 0.94 10-10 and 0.26 10-10mg-O2/min/cell for Vero cells and BOEC, respectively. Based on the oxygen consumption data, the phenomena of optimum oxygen tension required in embryo development in vitro has been analyzed, and we suggested that gas phase oxygen concentration, oxygen consumption rate of feeder cells and the number of feeder cells should be considered for the design of optimal co-culture system for effective fertilization of embryos in vitro.

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Streptomyces coelicolor의 연속 배양시 산소 분압에 따른 방어 효소의 활성 변화 (Effect of Partial Oxygen Pressure on the Growth and Defense Enzyme Activities of Streptomyces coelicolor in continuous culture system)

  • 박용두;이계준;노정혜
    • 한국미생물·생명공학회지
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    • 제22권5호
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    • pp.538-543
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    • 1994
  • Effect of partial oxygen pressure on the cell growth and the activities of oxidative defense enzymes were measured in the continuous culture of Streptomyces coelicolor. Both the wild type and the mutant strain resistant to hydrogen peroxide were cultured and the dry cell weight of the two cultures were measured at different oxygen tensions. Growth of the wild type was inhibited by oxygen at above 0.5 vvm. Growth of the hydrogen peroxide resistant mutant was stimulated by pure oxygen at 0.5 vvm but was inhibited by oxygen at 1.0 vvm. Therefore, growth of the hydrogen peroxide resistant mutant was less affected by the deleterious oxidative stress of oxygen. Activities of the several defense enzymes were also measured at different oxygen tensions. Activities of catalase and glucose-6-phosphate dehydrogenase increased significantly as oxygen pressure increased in the wild type culture. In the mutant, however, increase in those enzyme activities was not obvious whereas the uninduced levels of the above enzymes were higher than those of wild type. As judged by Western blotting, the amount of the major catalase increased as the oxygen pressure increased. This indicates that the induction of the catalase activity by oxygen pressure is mostly due to the increase in the expression level for the major catalase.

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셀레늄을 활용한 니켈철 (옥시)수산화물의 격자 산소 활성화 (Lattice Oxygen Activation in NiFe (Oxy)hydroxide using Se)

  • 조승환;손정인
    • 한국재료학회지
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    • 제32권8호
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    • pp.339-344
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    • 2022
  • The lattice oxygen mechanism (LOM) is considered one of the promising approaches to overcome the sluggish oxygen evolution reaction (OER), bypassing -OOH* coordination with a high energetic barrier. Activated lattice oxygen can participate in the OER as a reactant and enables O*-O* coupling for direct O2 formation. However, such reaction kinetics inevitably include the generation of oxygen vacancies, which leads to structural degradation, and eventually shortens the lifetime of catalysts. Here, we demonstrate that Se incorporation significantly enhances OER performance and the stability of NiFe (oxy)hydroxide (NiFe) which follows the LOM pathway. In Se introduced NiFe (NiFeSe), Se forms not only metal-Se bonding but also Se-oxygen bonding by replacing oxygen sites and metal sites, respectively. As a result, transition metals show reduced valence states while oxygen shows less reduced valence states (O-/O22-) which is a clear evidence of lattice oxygen activation. By virtue of its electronic structure modulation, NiFeSe shows enhanced OER activity and long-term stability with robust active lattice oxygen compared to NiFe.

Effect of Ce0.9Gd0.1O1.95 as a promoter upon the oxygen transfer properties of MgMnO3-δ-Ce0.9Gd0.1O1.95 composite oxygen carrier materials for chemical looping combustion

  • Hwang, Jong Ha;Lee, Ki-Tae
    • Journal of Ceramic Processing Research
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    • 제20권1호
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    • pp.18-23
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    • 2019
  • Chemical looping combustion (CLC) is a promising carbon capture and storage (CCS) technology whose efficiency and cost primarily relies on the oxygen carrier materials used. In this paper, gadolinium-doped ceria (GDC, Ce0.9Gd0.1O1.95) was added as a promoter to improve the oxygen transfer rate of MgMnO3-δ oxygen carrier materials. Increasing GDC content significantly increased the oxygen transfer rate of MgMnO3-δ-GDC composites for the reduction reaction due to an increase in the surface adsorption of CH4 via oxygen vacancies formed on the surface of the GDC. On the other hand, the oxygen transfer rate for the oxidation reaction decreased linearly with increasing GDC content due to the oxygen storage ability of GDC. Adsorbed oxygen molecules preferentially insert themselves into oxygen vacancies of the GDC lattice rather than reacting with (Mg,Mn)O to form MgMnO3-δ during the oxidation reaction.

Automatic Control of Fraction of Inspired Oxygen in Neonatal Oxygen Therapy using Fuzzy Logic Control

  • Chanyagorn, Pornchai;Kiratiwudhikul, Phattaradanai
    • IEIE Transactions on Smart Processing and Computing
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    • 제5권2호
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    • pp.107-116
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    • 2016
  • Premature babies of less than 37 weeks gestation might require oxygen therapy as an integral part of treatment and respiratory support. Because of their under-developed lungs, these so-called "preemies" might contract respiratory distress syndrome (RDS). To treat RDS, neonatal oxygen therapy is administered, where controlled oxygen gas is measured as a fraction of inspired oxygen ($FiO_2$). However, exposure to high oxygen content during long treatment could cause oxygen intoxication, which might cause permanent blindness due to retinopathy of prematurity (ROP), whereas insufficient oxygen exposure could cause severe hypoxia. A doctor would use oxygen saturation ($SpO_2$) data and prescribe a dose of $FiO_2$ to maintain $SpO_2$ within a suitable range. One objective is to maintain $SpO_2$ within the acceptable range using $FiO_2$ that is as low as possible. Adjustment of $FiO_2$ would normally be done by nurses every 15 to 30 minutes, which might not be safe in many situations. An error in $FiO_2$ adjustment during a manual procedure could be as large as +/- 2.5%. This paper presents a system that can determine an $FiO_2$ value suitable to the current $SpO_2$ and that automatically adjusts $FiO_2$ with an error clearance of +/- 0.25%.

미생물 배양 시스템에서의 산소 전달 (Oxygen Transfer in Microbial System)

  • 최동원
    • 한국식품영양학회지
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    • 제7권4호
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    • pp.399-405
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    • 1994
  • Some method was introduced to explain oxygen transfer from broth to cell during aerobic microbe cultivation. It is explained by 5 steps that how desolved oxygen can reach to cell. Among these steps film resistance was the most important factor to describe oxygen transfer. Lumped model and distributed model was introduced to explain oxygen diffusion rate and oxygen consumption rate which occurs in the microbe pellet.

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항생물질발효에서의 산소전달 및 흡수속도에 관한 연구 (The Oxygen Transfer and Oxygen Uptake in Antibiotic Fermentation using Streptomyces kanamyceticus)

  • Lee, Kye-Joon;Moo Bae
    • 한국미생물·생명공학회지
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    • 제10권3호
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    • pp.223-226
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    • 1982
  • 통기발효에서 중요한 통기 및 진탕효과를 측정함에 있어 공기로부터 물속으로 용존해 가는 산소의 전달속도와, 용존된 산소가 미생물에 의해 흡수되는 속도를 산출하였다. 항생물질발효에서 통기효과는 현저하였으며, 배양시간에 따른 산소흡수 속도와 비산소흡수 속도가 변화하는 것을 알았다. 이러한 변화는 대사이차 산물인 항생물질생합성의 생화학적인 전환에 기인하는 것으로 판단된다.

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다기능 산소전극에 의한 산소투과특성 동시측정 (Simultaneous measurement of oxygen permeability by using of multi-functional oxygen electrode)

  • 이동희;정진휘;유형풍;김태진
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2000년도 하계학술대회 논문집
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    • pp.532-535
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    • 2000
  • We have fabricated a sensor system for on-line monitoring the oxygen permeability and diffusivity of six different polymer films using the miniaturized 6 cathode(Ag)-single anode(Ag/AgCl) type hexagonal oxygen electrode. This system consists of multiple input front-end electronics, signal conditioning circuit using the embedded microcontroller 80C196KC, PC interface circuit and PC with the OS for microcontroller and the operating program for this system. The digital low-[ass filter was programmed and the simulated filter characteristics were enough to eliminate the noise from sensor signal. According to the experimental results, the linearity coefficients of the output voltage to oxygen partial pressure for each sensor electrode of six cathode type oxygen sensor are 0.998, 0.997, 0.998, 0.997, 0.997, 0.997 respectively, and the response times are all within 4 minutes.

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On a Reading Aloud to Relieve the Decrease in Blood Oxygen Saturation when Jogging

  • Tian, Zhixing;Bae, MyungJin
    • International Journal of Advanced Culture Technology
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    • 제8권4호
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    • pp.95-100
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    • 2020
  • Recently, the problem of hypoxia caused by jogging is attracting attention. To solve this problem, this paper proposed a new solution. This paper proved that as a vocalization method of reading aloud, it is possible to increase air intake and activate lung function to exchange more air and obtain more oxygen. Then, blood oxygen saturation was used as an evaluation index for the body's oxygen content level to prove its effectiveness. A photoelectric pulse oximeter developed on the basis of different light absorption principles in blood was used to test blood oxygen saturation. Experimental results show that a certain degree of hypoxia is induced when a lot of oxygen is required due to jogging. Therefore, it was proved that the new vocal breathing method by reading books can increase the blood oxygen saturation of the body and improve the hypoxia of the body. Reading vocal breathing is a simple and efficient oxygen saturation recovery breathing method.

광자기 디스크의 기록 및 자기적 특성에 산소가 미치는 영향 (Effect of Oxygen on the Magnetic and Recording Characteristics of Magneto-Optical Disk)

  • 최건
    • 한국자기학회지
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    • 제3권3호
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    • pp.229-233
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    • 1993
  • 광자기 디스크의 제조시 기록막내부의 산소함량에 따른 기록 및 자기적 특성의 변화를 연구하였다. 기록막내의 산소함량 변화는 반응성 스퍼터링에 의해 조절하였으며 스퍼터링 전과정 동안 산소의 거동을 in-situ 잔류가스분석기로 조사하였다. 산소함량의 증가에 따라 보자력은 감 소하였으나 수직이방성 및 수직 각형비등은 변화하지 않았다. 대부분의 첨가된 산소는 스퍼터링 과정중 소모되어 Tb원자와 안정한 산화물을 형성하여 자기적 조성의 변화를 초래하였지만 임계기 록크기와 임계외부자계크기에만 약간의 변화를 가져왔을 뿐 디스크의 기록신호잡음비에는 별로 영향을 미치지 않았다. 또한 디스크의 신뢰도면에서도 크게 감소하지 않았다.

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