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Electrical Stimulation Parameters in Normal and Degenerate Rabbit Retina (정상 망막과 변성 망막을 위한 전기자극 파라미터)

  • Jin, Gye-Hwan;Goo, Yong-Sook
    • Progress in Medical Physics
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    • v.19 no.1
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    • pp.73-79
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    • 2008
  • Retinal prosthesis is regarded as the most feasible method for the blind caused by retinal diseases such as retinitis pigmentosa (RP) or age related macular degeneration (AMD). Recently Korean consortium launched for developing retinal prosthesis. One of the prerequisites for the success of retinal prosthesis is the optimization of the electrical stimuli applied through the prosthesis. Since electrical characteristics of degenerate retina are expected to differ from those of normal retina, we performed voltage stimulation experiment both in normal and degenerate retina to provide a guideline for the optimization of electrical stimulation for the upcoming prosthesis. After isolation of retina, retinal patch was attached with the ganglion cell side facing the surface of microelectrode arrays (MEA). $8{\times}8$ grid layout MEA (electrode diameter: $30{\mu}m$, electrode spacing: $200{\mu}m$, and impedance: $50k{\Omega}$ at 1 kHz) was used to record in-vitro retinal ganglion cell activity. Mono-polar electrical stimulation was applied through one of the 60 MEA channel, and the remaining channels were used for recording. The electrical stimulus was a constant voltage, charge-balanced biphasic, anodic-first square wave pulse without interphase delay, and 50 trains of pulse was applied with a period of 2 sec. Different electrical stimuli were applied. First, pulse amplitude was varied (voltage: $0.5{\sim}3.0V$). Second, pulse duration was varied $(100{\sim}1,200{\mu}s)$. Evoked responses were analyzed by PSTH from averaged data with 50 trials. Charge density was calculated with Ohm's and Coulomb's law. In normal retina, by varying the pulse amplitude from 0.5 to 3V with fixed duration of $500{\mu}s$, the threshold level for reliable ganglion cell response was found at 1.5V. The calculated threshold of charge density was $2.123mC/cm^2$. By varying the pulse duration from 100 to $1,200{\mu}s$ with fixed amplitude of 2V, the threshold level was found at $300{\mu}s$. The calculated threhold of charge density was $1.698mC/cm^2$. Even after the block of ON-pathway with L-(1)-2-amino-4-phosphonobutyric acid (APB), electrical stimulus evoked ganglion cell activities. In this APB-induced degenerate retina, by varying the pulse duration from 100 to $1200{\mu}s$ with fixed voltage of 2 V, the threshold level was found at $300{\mu}s$, which is the same with normal retina. More experiment with APB-induced degenerate retina is needed to make a clear comparison of threshold of charge density between normal and degenerate retina.

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Survey of ICT Apply to Plastic Greenhouse, Rack·Pinion Adaption to Single Span and CFD Analysis (온실 ICT융복합 실태조사와 복숭아형 랙피니언천창 적용 단동온실 및 CFD 유동해석)

  • Cho, Kyu Jeong;Kim, Ki Young;Yang, Won Mo
    • Journal of Bio-Environment Control
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    • v.24 no.4
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    • pp.308-316
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    • 2015
  • This study was conducted to investigate the situation of ICT apply to plastic greenhouse, and the results be apply to design of new one. A CFD analysis were conducted to monitering the ventilation and energy saving of the single span greenhouse newly designed. The causes of delay to apply ICT to plastic greenhouse are the high cost for installation(24%), insufficiency of after services(19%), often disorder(16%), unskillful management of soft ware(15%), insufficient ICT efficiency(13%) and unsatisfying of income increase(12%). The parts of problem occurred in ICT plastic greenhouse are the structure, actuator, environmental control system and sensor(approximate 14%, respectively), remote control technique(13%), plant management technique(12%), energy saving technique(10%) and utilization of software(8%). In the condition of lateral window closed, the average wind speed changed to slow, but it was faster in the condition of leeward side window opened than in the condition of lee and winward side window opened. The air movement in the condition of lateral window closed occur by air moving fan not by out air. It is not affect the room temperature but effective the uniformity of room temperature. The average temperature of low height greenhouse was uniform than high height one. The average temperature in condition of 3rd curtain opened become same with outside temperature after 2 hours, but take more 5 hours in condition of 3rd curtain closed.

Usefulness of the Salivagram for the Diagnosis of Brain Lesions in Patients with Aspiration Pneumonia (뇌병변 환자에서 흡인성 폐렴 진단을 위한 Salivagram의 유용성)

  • Oh, Shin Hyun;Choi, Yung Sook;Ro, Dong Wook;Nam-Koong, Hyuk;Kim, Jae Sam;Leee, Chang Ho
    • The Korean Journal of Nuclear Medicine Technology
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    • v.17 no.1
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    • pp.48-52
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    • 2013
  • Purpose: Bed, living a long time is required in adult patients with brain lesions such as stroke, traumatic brain injury, and Parkinson's disease, causing pneumonia and respiratory diseases may be due to aspiration of food or saliva. In patients with recurrent pneumonia or pulmonary symptoms, there is a need to determine the possibility of pulmonary aspiration due to aspiration of saliva. Materials and Methods: Saliva due to aspiration pneumonia diagnosis in patients with brain lesions request for inspection to the Department of Nuclear Medicine, 10 patients (male 6, female 4) were included in this study. Patients were fasted before the test, $^{99m}Tc_{O4}$ 185 MBq (5 mCi) of less than 1 mL of solution was administered in the oral cavity. Administration and 20 minutes of dynamic imaging acquisition, and immediately after that the static images were acquired. Delayed scan after 2-4 hours if necessary. Results: Positivity rate of all 10 patients was 60%. In 4 patients showed positive reactions after the administration of oral cavity in a 20-minute dynamic imaging were able to confirm whether the aspiration. In the remaining 2 patients, four hours of additional delay tests were able to confirm whether the aspiration. Conclusion: Does not require changes in patient posture compared to the other checks that can be diagnosed with aspiration pneumonia. A simple test and takes less time. Therefore be useful in providing information for the diagnosis and treatment modality.

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A Study of Domain Name Disputes Resolution with the Korea-U.S. FTA Agreement (한미자유무역협정(FTA)에 따른 도메인이름 분쟁해결의 개선방안에 관한 연구)

  • Park, Yu-Sun
    • Journal of Arbitration Studies
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    • v.17 no.2
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    • pp.167-187
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    • 2007
  • As Korea has reached a free trade agreement with the United States of America, it is required to provide an appropriate procedure to ".kr" domain name disputes based on the principles established in the Uniform Domain Name Dispute Resolution Policy(UDRP). Currently, Internet address Dispute Resolution Committee(IDRC) established under Article 16 of the Act on Internet Address Resources provides the dispute resolution proceedings to resolve ".kr" domain name disputes. While the IDRC's proceeding is similar to the UDRP administrative proceeding in procedural aspects, the Domain Name Dispute Mediation Policy that is established by the IDRC and that applies to disputes involving ".kr" domain names is very different from the UDRP for generic Top Level Domain (gTLD) in substantial aspects. Under the Korea-U.S. Free Trade Agreement(KORUS FTA), it is expected that either the Domain Name Dispute Mediation Policy to be amended to adopt the UDRP or the IDRC to examine the Domain Name Dispute Mediation Policy in order to harmonize it with the principles established in the UDRP. It is a common practice of cybersquatters to warehouse a number of domain names without any active use of these domain names after their registration. The Domain Name Dispute Mediation Policy provides that the complainant may request to transfer or delete the registration of the disputed domain name if the registrant registered, holds or uses the disputed domain name in bad faith. This provision lifts the complainant's burden of proof to show the respondent's bad faith because the complainant is only required to prove one of the three bad faiths which are registration in bad faith, holding in bad faith, or use in bad faith. The aforementioned resolution procedure is different from the UDRP regime which requires the complainant, in compliance with paragraph 4(b) of the UDRP, to prove that the disputed domain name has been registered in bad faith and is being used in bad faith. Therefore, the complainant carries heavy burden of proof under the UDRP. The IDRC should deny the complaint if the respondent has legitimate rights or interests in the domain names. Under the UDRP, the complainant must show that the respondent has no rights or legitimate interests in the disputed domain name. The UDRP sets out three illustrative circumstances, any one of which if proved by the respondent, shall be evidence of the respondent's rights to or legitimate interests in the domain name. As the Domain Name Dispute Mediation Policy provides only a general provision regarding the respondent's legitimate rights or interests, the respondent can be placed in a very week foundation to be protected under the Policy. It is therefore recommended for the IDRC to adopt the three UDRP circumstances to guide how the respondent can demonstrate his/her legitimate rights or interests in the disputed domain name. In accordance with the KORUS FTA, the Korean Government is required to provide online publication to a reliable and accurate database of contact information concerning domain name registrants. Cybersquatters often provide inaccurate contact information or willfully conceal their identity to avoid objection by trademark owners. It may cause unnecessary and unwarranted delay of the administrative proceedings. The respondent may loss the opportunity to assert his/her rights or legitimate interests in the domain name due to inability to submit the response effectively and timely. The respondent could breach a registration agreement with a registrar which requires the registrant to submit and update accurate contact information. The respondent who is reluctant to disclose his/her contact information on the Internet citing for privacy rights and protection. This is however debatable as the respondent may use the proxy registration service provided by the registrar to protect the respondent's privacy.

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Seed Viability and Growth Characteristics of Eclipta prostrata (L.) L. (한련초의 종자생존력(種子生存力) 및 생장특성(生長特性))

  • Lee, H.K.;Moody, K.
    • Korean Journal of Weed Science
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    • v.8 no.3
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    • pp.309-316
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    • 1988
  • Several experiments were conducted to investigate the achene viability and growth characteristics of Eclipta prostrata (L.) L. No dormancy and no after-ripening requirement were found for E. prostrata achenes. When achenes were stored at room temperature, germination did not decrease with up to 5 months storage. Large differences in loss of viability of E. prostrata achenes occurred when different dehydration methods were used. Immediate dehydration resulted in high viability, but slow dehydration resulted in severe loss of viability. Achene viability at shallow burial depths (5 and 10 cm deep) was lower under upland soil conditions than under lowland soil conditions. Seedling growth was greatly reduced when flooding to a depth of 10 cm occurred at or before the 4-leaf stage. Flooding after the 4-leaf stage stimulated stem elongation. Branching started from the second week and usually terminated at the tenth week. Leaf size was determined by the branch which are related to the assimilate supply. Flowering of E. prostrata started during the fifth week after emergence, and mature achenes were produced from the sixth week. Ten to 14 days were needed for the achenes to mature. About 14,000 achenes were produced on each plant. Achene production per week increased from the sixth week to the tenth week and thereafter it declined. The average number of achenes per inflorescence decreased with delay in flowering.

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Comparison of Several Agronomic Characteristics as Affected by Seeding Dates (파종기 이동이 맥류의 실용적 저형질에 미치는 영향)

  • Hyung-Soo Suh
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.4
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    • pp.298-303
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    • 1981
  • A study was conducted to find out the varietal difference in some important agronomic characters in response to different seeding time in barely from 1975 to 1976 at Chikugo Agric. Expt. Station in Japan. Thirteen varieties which are diverse in growth period were used. Nine seeding time were tried. Seedling emergence, primary tillering, and formation of flower primodia were delayed in proportion to the seeding time. And also heading and maturing time showed the same tendency. This tendency appeared more conspicuous in the late maturing varieties. Therefore, days to heading and maturing duration were inversely shortened by the delay of seeding time and by earliness of variety. Number of spikes as well as culm length and spike length was reduced in the late seeding. Grain yield was reduced in all varieties by late seeding, easpecially with late maturing varieties. In this test, widely adaptable variety to late seeding time was not found, but earliness and high tillering in growth habit appeared more favorable to yield increase.

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A High-Eating Quality Rice Variety 'Cheonghaejinmi' Adaptable to Low Nitrogen Fertilizer Application (질소 소비료 적성 고양식미 벼 신품종 '청해진미')

  • Oh, Myung-Kyu;Kim, Yeon-Gyu;Kim, Myeong-Ki;Cho, Young-Chan;Hwang, Hung-Goo;Hong, Ha-Cheol;Choi, Im-Soo;Kim, Jeong-Ju;Lee, Jeom-Ho;Baek, Man-Kee;Choi, Yong-Hwan;Jeong, Jong-Min;Yang, Chang-In;Oh, Sea-Kwan;Choi, In-Bea;Won, Yong-Jae;Chun, A-Reum
    • Korean Journal of Breeding Science
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    • v.42 no.3
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    • pp.307-312
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    • 2010
  • 'Cheonghaejinmi' is a new japonica rice variety developed from three-way cross between Samjiyeon/SR14694-57-4-2-1-3-2-2//Iri402 by the rice breeding team of National Institute of Crop Science, RDA. Heading date of this variety is August 18, 4 days later than that of 'Sobibyeo' in middle plain areas. It has culm length of 78 cm, 125 spikelets per panicle, 92.5% of ripened grain rate, and 23.9 g of 1000-brown rice weight. It showed 12 days of heading delay, and 63% spikelet fertility in cold-water irrigation stress. 'Cheonghaejinmi' is susceptible to blast disease, bacterial blight, virus diseases and plant hoppers. The nitrogen use efficiency of this variety is higher than that of Sobibyeo in low nitrogen application level. Milled rice of 'Cheonghaejinmi' exhibits translucent, clear non-glutinous endosperm and medium short grain. It has 5.9% protein content, 20.3% amylose content, and 0.28 palatability index of cooked rice compared to -0.11 of Hwaseongbyeo. The milled rice yield of 'Cheonghaejinmi' was about 5.31 MT/ha at low nitrogen application level of ordinary season culture. This variety had 98.8% whole grain in milled rice and 76% milling recovery of whole grain. 'Cheonghaejinmi' would be adaptable to middle plain areas and middle-western coastal areas in Korea.

Effects of Seeding Date on Growth, Yield, and Fatty Acid Content of Perilla Inter-cropped with Sesame in Central Korea (중부지역 참깨 간작 들깨 재배시 파종기가 수량 및 품질에 미치는 영향)

  • Kim, Young Sang;Kim, Ki Hyeon;Yun, Cheol Gu;Heo, Yun Seon;Kim, Ik Jei;Kim, Young-Ho;Song, Yong-Sup;Lee, Myoung Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.2
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    • pp.138-145
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    • 2021
  • Perilla contains more than 60% of fatty acids. Linolenic acid is effective in preventing heart disease, improving learning ability, treating allergies, and preventing cancer. This study was carried out to improve the cultivation method to aid the stable production of perilla by developing a suitable inter-cropping system with sesame in the central region as well as to report a suitable planting time. The test results are summarized as follows. As the planting time of perilla in the inter-cropping system with sesame was delayed, the number of clusters and capsules decreased. The perilla yields in this system showed significant differences compared to that with the previous crops (sesame varieties) and planting period. The yield of perilla was significantly lower in the characteristic-Type B variety than in the characteristic-Type A variety and decreased significantly as the planting time was delayed. With regards to the quality characteristics of perilla, such as crude protein, crude fat, etc., there were no differences between previous perilla crops and those inter-cropped with sesame. The perilla composition did not show any difference during the planting period; however, with delay in the planting time, crude protein content increased but crude fat content decreased. Yield of perilla was 38% higher in a two-row (40 x 40 cm) system, compared to a single-row cultivation (110 x 20 cm) of perilla inter-cropped with sesame. These results suggest that the suitable method for inter-cropping perilla with sesame in the central region is to sow the characteristic-Type A variety in early May, and cultivate the perilla in two lines (40 x 40 cm) in mid-June. This was judged to be the best cultivation method in the central region.

An Experimental Study on the Hydration Heat of Concrete Using Phosphate based Inorganic Salt (인산계 무기염을 이용한 콘크리트의 수화 발열 특성에 관한 실험적 연구)

  • Jeong, Seok-Man;Kim, Se-Hwan;Yang, Wan-Hee;Kim, Young-Sun;Ki, Jun-Do;Lee, Gun-Cheol
    • Journal of the Korea Institute of Building Construction
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    • v.20 no.6
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    • pp.489-495
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    • 2020
  • Whereas the control of the hydration heat in mass concrete has been important as the concrete structures enlarge, many conventional strategies show some limitations in their effectiveness and practicality. Therefore, In this study, as a solution of controling the heat of hydration of mass concrete, a method to reduce the heat of hydration by controlling the hardening of cement was examined. The reduction of the hydration heat by the developed Phosphate Inorganic Salt was basically verified in the insulated boxes filled with binder paste or concrete mixture. That is, the effects of the Phosphate Inorganic Salt on the hydration heat, flow or slump, and compressive strength were analyzed in binary and ternary blended cement which is generally used for low heat. As a result, the internal maximum temperature rise induced by the hydration heat was decreased by 9.5~10.6% and 10.1~11.7% for binder paste and concrete mixed with the Phosphate Inorganic Salt, respectively. Besides, the delay of the time corresponding to the peak temperature was apparently observed, which is beneficial to the emission of the internal hydration heat in real structures. The Phosphate Inorganic Salt that was developed and verified by a series of the aforementioned experiments showed better performance than the existing ones in terms of the control of the hydration heat and other performance. It can be used for the purpose of hydration heat of mass concrete in the future.

A Prediction of N-value Using Artificial Neural Network (인공신경망을 이용한 N치 예측)

  • Kim, Kwang Myung;Park, Hyoung June;Goo, Tae Hun;Kim, Hyung Chan
    • The Journal of Engineering Geology
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    • v.30 no.4
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    • pp.457-468
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    • 2020
  • Problems arising during pile design works for plant construction, civil and architecture work are mostly come from uncertainty of geotechnical characteristics. In particular, obtaining the N-value measured through the Standard Penetration Test (SPT) is the most important data. However, it is difficult to obtain N-value by drilling investigation throughout the all target area. There are many constraints such as licensing, time, cost, equipment access and residential complaints etc. it is impossible to obtain geotechnical characteristics through drilling investigation within a short bidding period in overseas. The geotechnical characteristics at non-drilling investigation points are usually determined by the engineer's empirical judgment, which can leads to errors in pile design and quantity calculation causing construction delay and cost increase. It would be possible to overcome this problem if N-value could be predicted at the non-drilling investigation points using limited minimum drilling investigation data. This study was conducted to predicted the N-value using an Artificial Neural Network (ANN) which one of the Artificial intelligence (AI) method. An Artificial Neural Network treats a limited amount of geotechnical characteristics as a biological logic process, providing more reliable results for input variables. The purpose of this study is to predict N-value at the non-drilling investigation points through patterns which is studied by multi-layer perceptron and error back-propagation algorithms using the minimum geotechnical data. It has been reviewed the reliability of the values that predicted by AI method compared to the measured values, and we were able to confirm the high reliability as a result. To solving geotechnical uncertainty, we will perform sensitivity analysis of input variables to increase learning effect in next steps and it may need some technical update of program. We hope that our study will be helpful to design works in the future.