• Food Science and Biotechnology
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• 제14권5호
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• pp.599-603
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• 2005

Chemical constituents of fruit peels of Fortunella japonica Swingle were investigated, and ten compounds were purified and isolated through various chromatographic procedures. Through NMR analysis, isolated compounds were identified as ${\alpha}$-tocopherol (1), lupenone (2), ${\beta}$-amyrin (3), ${\alpha}$-amyrin (4), ${\beta}$-sitosterol (5), ${\beta}$-sitosteryl 3-O-glucopyranoside (6), kaempferide 3-O-rhanmopyranoside (7), 3',5'-di-C-${\beta}$-glucopyranosylphloretin (8), acacetin 7-O-neohesperidoside (9), and acacetin 8-C-neohesperidoside (10). Compounds 1-7 were identified for the first time by our group from fruit peels of F. japonica.

• Journal of Applied Biological Chemistry
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• 제54권4호
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• pp.309-312
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• 2011

Chipped stem wood from Lindera glauca was extracted repeatedly with 80% aqueous methanol at room temperature, and the concentrated methanolic extract was successively partitioned with ethyl acetate (EtOAc), n-butyl alcohol, and $H_2O$. From the EtOAc fraction, four sterols were isolated through a repeated silica gel and octadecyl silica gel column chromatography. The chemical structures of the sterols were elucidated as ${\beta}$-sitosterol (1), 7-ketositosterol (2), 7${\beta}$-hydroxysitosterol (3), and daucosterol (4). Among them, compounds 2 and 3 were isolated for the first time from the stem woods of this plant.

• Archives of Pharmacal Research
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• 제21권2호
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• pp.207-211
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• 1998

Three prolyl endopeptidase inhibitors were isolated and identified as luteolin, quercetin and ${\beta}$-sitosterol-3-O-${\beta}$-D-glucopyranoside with $IC_{50}$ of 0.17, 0.19 and 27.5 ppm, respectively. The inhibition of two flavonoids were non-competitive with substrate. Twenty authentic flavonoids were tested in order to investigate structure-activity relationship. No significant relationship was found in them, however, catechol moiety of B-ring and 7-OH group in flavonoid skeleton were seemed to be responsible for the stronger activity.

• Food Science and Biotechnology
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• 제14권5호
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• pp.604-607
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• 2005

In this study, the effect of ${\beta}$-sitosterol (SS) in the liposome bilayer on the stability of incorporated retinol was evaluated. Retinol was incorporated into liposomes consisting of various ratios of soybean phoaphatidylcholine (PC) to SS, while liposomes were prepared as multilamellar vesicles by the dehydration/rehydration method. Retinol was readily incorporated into liposomes with various SS contents, with incorporation efficiencies higher than 98% for all conditions. The incorporation efficiency of retinol increased slightly as the SS content in liposomes increased. Its average particle size also increased as the SS content increased. Mean particle size at PC to SS ratios of 100:0, 90:10, 80:20, 70:30, 60:40, and 50:50 were 12.16, 17.57, 35.00, 40.62, 83.45, and $88.94\;{\mu}m$, respectively. Liposomal retinol degradation in aqueous solution was measured with respect to SS content at various periods of time at four different temperatures of 4, 25, 37, and $50^{\circ}C$, and the stability of the incorporated retinol enhanced as the SS content increased. At $4^{\circ}C$, for example, retinol in the liposomes of 50:50 (PC:SS) remained at 84.42% after storage for 10 days, while in 100:0 (PC/SS) it remained at 42.62%. These results indicate that SS content in liposomes played an important role in the incorporation efficiency of retinol and its stability.

• Journal of Nutrition and Health
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• 제13권4호
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• pp.159-166
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• 1980

Methods for the determination of sterols in sesame oils were studied. The sesame oils were saponified and the sterols isolated from the unsaponifiable matter by Florisil column chromatography, and the individual components were determined by means of gas chromatography. Campesterol, ${\beta}-sitosterol$, stigmasterol were found in sesame oil including unknown Ⅰ and Ⅱ. The use of SE-30 gas chromatographic column allows the slow elution, duplication of peaks and relatively low reproducibility, therefore, 3% OV-17 was suitable for the sterol analysis. The result of this study showed that contents of sterols in sesame oil were campesterol 8.4%, stigmasterol 4.5%, ${\beta}-sitosterol$ 33.9% and others 53.0% involving 8.8% of unknown I and 44.3% of unknown Ⅱ. There has been no specific test available for identifying the sesame oil among common edible oils. But the ratio of sterols in sesame oils allowed the estimation of genuiness. The ratio of sterols vs. campesterol in genuine sesame oils were stigmasterol 0.3- 0.6, ${\beta}-sitosterol$ 3.0-3.8 and unknown Ⅱ 3.0, respectively. The 65 samples were composed of genuine sesame oil 40%, mixed rape seed oil 3%, cotton seed oil 1. 5% others were reused soybean oil or re-extracted oil.

• 생약학회지
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• 제8권4호
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• pp.149-153
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• 1977

Evodia daniellii $H_{EMSLEY}$ (Rutaceae) is cultivated in Korea and used as a folkmedicine for gastric inflammation, extermination of noxious insects, headache and antiinflammatory. The seed oil of this plant also has been used in various diseases, for example, dermatitis, scabies and so forth. Fructus Evodiae seed oil sterols were obtained from the nonsaponifiable matters of ethereal extract of the seed. The composition of sterols have been determined by gas liquid chromatography and thin layer chromatography on the preparative plates. It was noted that ${\beta}-sitosterol$ was the major sterols in the Evodia daniellii $H_{EMSLEY}$ in Korea. The results showed that contents of Evodia daniellii $H_{EMSLEY}$ sterols were campesterol 14.01%, stigmasterol 4.36%, ${\beta}-sitosterol$ 71.26%, ${\delta}^7-sterol$ 9.21% and unknown sterol 1.16% by method of triangulation and campesterol 13.02%, stigmasterol 3.87%, ${\beta}-sitosterol$ 74.34%, ${\delta}^7-sterol$ 8.67% and unknown sterol 1.85% by method of planimetry.

• 생약학회지
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• 제38권4호
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• pp.354-357
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• 2007

Three compounds (1-3) were isolated from the roots of Potentilla discolor (Rosaceae). The structure of compounds 1-3 were elucidated as stigmast-5-en-3-ol $({\beta}-sitosterol,\;1),\;2,19{\alpha}-dihydroxy$-2-oxo-urs-1,12-dien-28-oic acid (fupenjic acid, 2), 3-O-${\beta}$-D-glucopyranosylstigmast-5-en-3-ol (${\beta}-sitosterol\;{\beta}-D-glucopyranoside, 3) based on physical and spectroscopic data. $^{13}C-NMR$ assignments were completed by 2D-NMR technique. The three compounds were firstly isolated from Potentilla discolor.

• 한국식품영양과학회지
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• 제39권7호
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• pp.980-985
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• 2010

이번 연구는 강원도 춘천에서 재배된 비타민나무의(Hippophae rhamnoides L.) 부위별 추출물을 대상으로 항염활성 작용에 미치는 영향을 구명하였고, 이에 함유되어 있는 항염활성물질을 fraction directed isolation 방법으로 단리하였다. 항염활성 측정을 위하여 비타민나무의 줄기, 잎, 뿌리, 열매의 에탄올 추출물에 대하여 nitric oxide(NO) 생성량 측정 결과 염증 유발 물질인 lipopolysaccharide(LPS) 대비 각각 56.0, 31.9, 49.1, 18.9%의 저해 활성을 나타내었다. 비타민나무 줄기를 다시 극성을 달리하여 용매로 분획을 실시하였고 각각의 분획층에 대하여 NO 생성량 측정 결과 $CH_2Cl_2$ 분획층(1 mg/mL)에서 염증유발물질인 LPS 단독 처리군 대비 80%의 높은 항염활성을 나타내었다. 이에 항염활성이 가장 높았던 dichloromethane 분획층을 MCA-MCK까지 총 11개 분획층으로 분리하였고, 이 11개의 분획층에 대하여 NO 생성량을 측정한 결과 MCD 분획층이 0.1 mg/mL 농도에서 LPS 대비 65%의 가장 높은 항염활성을 나타내었다. MCD의 구조를 밝히기 위하여 GC/MS, $^{13}C$-NMR, $^1H$-NMR 분석을 수행한 결과, 항염활성물질 MCD는 분자량 414.71 g/mol, 화학구조식이 $C_{29}H_{50}O$인 $\beta$-sitosterol로 결정하였다. 향후 비타민나무 줄기는 염증예방 효능을 지닐 수 있는 기능성식품 및 의약품의 원료, 가공품 등 다양하게 활용될 수 있을 것이라 사료된다.

• Applied Biological Chemistry
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• 제49권1호
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• pp.82-85
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• 2006

산딸나무 열매를 100% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 3종의sterol 화합물을 분리, 정제하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ${\beta}-sitosterol$(1), $stigmast-4-en-6{\beta}-ol-3-one$(2), daucosterol(3)으로 동정하였다. 이 화합물들은 산딸나무에서 처음 분리되었다.

• 한국식품영양과학회지
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• 제45권11호
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• pp.1580-1588
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• 2016

잔대의 70% 에탄올 추출물은 강한 항산화 효과 및 산화적 스트레스 조건에서 뇌신경세포 사멸 억제 효과가 있었다. 6개의 분획물 중에 잔대 추출물의 클로로포름 분획물(AT-CH)은 높은 DPPH 라디칼 소거작용 및 세포 내 활성산소종(ROS) 소거작용을 가지고 있었고 $H_2O_2$에 대항하여 뇌신경 세포(SK-N-SH) 보호 효과가 가장 뛰어났다. 활성이 가장 우수한 클로로포름 분획물을 대상으로 주요 물질을 순수분리한 후 $^1H-NMR$, $^{13}C-NMR$ 그리고 EI mass spectra를 이용하여 화학구조를 분석한 결과 phytosterols(${\beta}$-sitosterol과 daucosterol)이라는 것을 알 수 있었다. ${\beta}$-Sitosterol과 daucosterol 역시 산화적 스트레스에 대항하여 SK-N-SH 세포를 보호하는 작용이 있었다. AT-CH 그리고 이것으로부터 분리된 화합물은 p38 경로를 저해하였다. 이들 결과는 AT-CH는 p38 경로를 저해하고 세포 내 ROS 소거작용에 의해 산화적 스트레스로부터 뇌신경세포 보호 효과를 가질 것이라 제안하였다.