• Title/Summary/Keyword: analysis and synthesis

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Functional Analysis of the Invariant Residue G791 of Escherichia coli 16S rRNA

  • Song, Woo-Seok;Kim, Hong-Man;Kim, Jae-Hong;Sim, Se-Hoon;Ryou, Sang-Mi;Kim, Sang-Goo;Cha, Chang-Jun;Cunningham, Philip R.;Bae, Jee-Hyeon;Lee, Kang-Seok
    • Journal of Microbiology
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    • v.45 no.5
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    • pp.418-421
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    • 2007
  • The nucleotide at position 791(G791) of E. coli 16S rRNA was previously identified as an invariant residue for ribosomal function. In order to characterize the functional role of G791, base substitutions were introduced at this position, and mutant ribosomes were analyzed with regard to their protein synthesis ability, via the use of a specialized ribosome system. These ribosomal RNA mutations attenuated the ability of ribosomes to conduct protein synthesis by more than 65%. A transition mutation (G to A) exerted a moderate effect on ribosomal function, whereas a transversion mutation (G to C or U) resulted in a loss of protein synthesis ability of more than 90%. The sucrose gradient profiles of ribosomes and primer extension analysis showed that the loss of protein-synthesis ability of mutant ribosomes harboring a base substitution from G to U at position 791 stems partially from its inability to form 70S ribosomes. These findings show the involvement of the nucleotide at position 791 in the association of ribosomal subunits and protein synthesis steps after 70S formation, as well as the possibility of using 16S rRNA mutated at position 791 for the selection of second-site revertants in order to identify ligands that interact with G791 in protein synthesis.

Efficient Dynamic Response Analysis of Structures Supported By Nonlinear Resilient Mounts Using Structural Synthesis Method (구조합성법을 이용한 비선형 탄성마운트 지지 구조물의 효율적인 동적 응답 해석)

  • Chung, J.H.;Kim, B.H.;Yang, Y.J.
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2000.11a
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    • pp.287-290
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    • 2000
  • An efficient dynamic response analysis method of structures supported by nonlinear resilient mounts when subjected to the transient base excitations is presented by using the structural synthesis method in time domain. Through a numerical example, the validity of the presented method is verified by comparison of the results with those of the 'traditional' analysis method.

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Efficient Synthesis of Bibenzyl Derivatives Bearing Pyranyl Moieties: First Total Synthesis of Bauhinol D

  • Park, Byung-Ho;Lee, Yong-Rok;Kim, Sung-Hong
    • Bulletin of the Korean Chemical Society
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    • v.32 no.2
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    • pp.566-570
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    • 2011
  • An efficient and general synthesis of bibenzyl derivatives bearing pyranyl rings was achieved by ethylenediamine diacetate-catalyzed reactions of 3,5-dihydroxybibenzyl with ${\alpha},{\beta}$-unsaturated aldehydes in moderate yields. These reactions provided naturally occurring compounds 1 and 2 in single step reaction. As an application of this methodology, biologically interesting natural bauhinol D (8) was synthesized by a convergent sequence from readily available benzaldehyde and benzyl phosphonate.

Finite Element Analysis of the Self-propagating High-temperature Synthesis Of $MoSi_2$ ($MoSi_2$ 자전 고온 반응 합성의 유한 요소 해석)

  • 채수원;손창현;김용석
    • Journal of the Korean Society for Precision Engineering
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    • v.15 no.12
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    • pp.120-127
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    • 1998
  • The finite element method has been used to model and analyze the heat transfer phenomena during manufacturing process of $MoSi_2$ by SHS(Self-propagating High-temperature Synthesis). For this purpose nonlinear transient heat transfer analyses by using ANSYS have been performed to estimate the temperature distributions and the peak temperature in the test specimen. The effects of manufacturing process parameters such as pre-heating temperatures, the velocity of reaction zone have been investigated. The results of the analysis have been compared with the experimental results.

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A Knowledge-Based CAD System for Gate in Injection Molding (사출성형 게이트 설계용 지식형 CAD 시스템)

  • 허용정
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.2 no.2
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    • pp.33-37
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    • 2001
  • The synthesis of gates of injection-molded parts has been done empirically, since it requires profound knowledge about the gate design,. which is not available to designers through current CAD systems. GATEWAY is a knowledge module which contains knowledge to Permit non-experts as well as mold design experts to generate acceptable gate design of injection-molded parts. A knowledge-based CAD system is constructed by adding the knowledge module, GATEWAY, for gate synthesis and appropriate CAE programs for mold design analysis to an existing geometric modeler to provide designers, at the initial stage, with comprehensive process knowledge for gate synthesis. Performance analysis and feature-based geometric modeling.

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Dynamic analysis of a flexible multibody system

  • Chae Jang-Soo;Park Taw-Won;Kim J.
    • International Journal of Precision Engineering and Manufacturing
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    • v.6 no.4
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    • pp.21-25
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    • 2005
  • In the dynamic analysis of a mechanism, if one or more of the components are flexible, then the simulation will not be accurate because of the violation of the rigid body assumption. Mode shapes are used to represent the dynamic behavior of an elastic structure. A modal synthesis method which uses a combination of normal modes, constraint modes, and attachment modes, was used to represent effectively the elastic deformation of a flexible multibody. Since the combination of these modes should be different for each type of connecting part, the modal synthesis method was studied for the various types of interconnecting joints. In addition, the analysis procedure for the flexible body was explained. A satellite system with flexible solar panels was chosen as an example to show the effectiveness of the proposed method.

Synthesis and Analysis of Optical Transfer Function of the Modified Triangular Interferometer by Two - pupil Synthesis Method

  • Kim, Soo-Gil
    • Journal of the Optical Society of Korea
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    • v.8 no.4
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    • pp.182-187
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    • 2004
  • We synthesized and analyzed the optical transfer function(OTF) of the modified triangular interferometer (MTI) using two-pupil synthesis method. Also, we presented the optimal MTI, which can obtain any bipolar function by combining two wave plates and a linear polarizer. By using the proposed MTI, we can obtain the complex hologram without bias and conjugate image.

On Altering the Pitch of Speech Signals in Waveform Coding -(Altering Method by the LPC and the Pitch Halving)- (음성 파형코딩의 음원피치 변경에 관한 연구 - LPC와 주기반분법에 의한 피치변경법 -)

  • 민경중
    • Proceedings of the Acoustical Society of Korea Conference
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    • 1991.06a
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    • pp.45-49
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    • 1991
  • In area of the speech synthesis, the waveform coding with high quality are mainly used to the synthesis by analysis. However, it is difficult to applying the waveform coding to the synthesis by rule, because the parameters of this coding are not classified as either excitation parameters and vocal tract parameters. In this paper, we proposed a new pitch change method that can alter the pitch periods in the waveform coding. The proposed method expands the pitch period by the LPC synthesis method, and then the period is compressed by the waveform halving technique. Thus, it is possible that the waveform coding is carried out the synthesis by rule in speech processing.

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Effect of 20-Hydroxyecdysone on Yolk Protein Synthesis in Drosophila sp. (robusta Species Group) (제주먹초파리에서 난황 단백질 합성에 미치는 20-Hydroxyecdysone의 영향)

  • 김세재;김기옥오문유이정주
    • The Korean Journal of Zoology
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    • v.35 no.3
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    • pp.271-276
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    • 1992
  • 20-hydroxvecdysone (20-HE) seems to be related in the regulation of vitellogenesis in Drosophifa sp. (robwta species sroup). Although yolk proteins (YPs) synthesis does not occur at a high rate in fat body cells of one daw-old female after eclosion, application of 20-HE to isolated abdomens deprived of anterior endocrine glands stimulated the synthesis and secretion of YPs into the hemolvmph. An injection of 0.3 $\mul$ of a 10-s M 20-HE was sufficient topromote synthesis and secretion of YPs in isolated abdomens. The response of isolated automens to hormones was first detected between 2 hr and 3 hr after treaDent of 10-s M 20-HE. Transcript analysis showed that the effect of 20-HE on yolk protein synthesis was mediated at the level of transcription.

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