• Title/Summary/Keyword: anaerobic

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Isolation of Hydrogen-producing Bacteria from Granular Sludge of an Upflow Anaerobic-Sludge Blanket Reactor

  • Oh, You-Kwan;Park, Mi-So;Seol, Eun-Hee;Lee, Sang-Joon;Park, Sunghoon
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.1
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    • pp.54-57
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    • 2003
  • H$_2$-producing bacteria were isolated from anaerobic granular sludge. Out of 72 colonies (36 grown under aerobic conditions and 36 under anaerobic conditions) arbitrarily chosen from the agar plate cultures of a Suspended sludge, 34 colonies (15 under aerobic conditions and 19 under anaerobic conditions) produced H2 under anaerobic conditions. Based on various biochemical tests and microscopic observations, they were classified into 13 groups and tentatively identified as follows: From aerobic isolates, Aeromonar spp. (7 strains), Pseudomonas spp. (3 strains), and Vibrio spp. (5 strains); from anaerobic isolates, Actinomyces spp. (11 Strains), Clostridium 5pp. (7 strains). and Porphyromonas sp. When glucose was used as the carbon substrate, all isolates showed a similar cell density and a H$_2$ production yield in the batch cultivations after 12 h (2.24-2.74 OD at 600 nm and 1.02-1.22 mol H$_2$/mol glucose, respectively). The major fermentation by-products were ethanol and acetate for the aerobic isolates, and ethanol, acetate and propionate for the anaerobic isolates. This study demonstrated that several H$_2$ producers in an anaerobic granular sludge exist En large proportions and their performance in terms of H$_2$ production is quite similar.

Status of Anaerobic Digestion Facility for Pig-slurry in Korea (국내 가축분뇨 혐기소화시설 현황 및 운영실태 분석)

  • Jeong, Kwang-Hwa;Kim, Jung-Gon;Han, Duk-Woo;Kwag, Jung-Hun
    • Journal of Animal Environmental Science
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    • v.20 no.1
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    • pp.27-34
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    • 2014
  • This study was conducted to survey and inspect the currently operating anaerobic digestion facilities for animal manure. Recently, the number of anaerobic digestion facility for livestock manure is on the rise thanks to growing interest in developing alternative energy. A anaerobic digestion system has been constructed in large scale farms or animal manure public resource center. Currently, most animal manure anaerobic digestion facilities in operation are producing biogas from the pig slurry which contains 97% water. Methane gas can be used to operate a engine generator which then produces electricity. Anaerobic digestate, a by-product of digestion, is mostly utilized as a liquid fertilizer after curing processing. Only in a few cases, it can be discharged after wastewater treatment process. The problem of anaerobic digestate treatment is the imbalance of C/N ratio. The content of N was too high to keep it into normal process.

Anaerobic Degradation of Aromatic Compounds by Microorganisms in Paddy Field

  • Katayama, A.;Yoshida, N.;Shibata, A.;Baba, D.;Yang, S.;Li, Z.;Kim, H.;Zhang, C.;Suzuki, D.
    • 한국환경농학회:학술대회논문집
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    • 2011.07a
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    • pp.128-135
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    • 2011
  • Consortia demonstrated the high capacities of anaerobic degradation of various aromatic compounds, which were successfully enriched from gley paddy soils under different conditions. Phenol and cresol was decomposed anaerobically using nitrate, ferric oxide or sulfate as electron acceptors. Biphenyl was degraded to $CO_2$, especially without addition of external electron acceptor. Alkylphenols with middle length of alkyl chain, were co-metaboliocally degraded with the presence of hydroxylbenzoate as the co-substrate under nitrate reducing conditions. The microorganisms responsible for the anaerobic co-metabolism was Thauera sp. Reductive dechlorination activity was also observed for polychlorophenols, fthalide, polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins with the presence of lactate, formate or $H_2$ as electron donor. The fthalide dechlorinator was classified as Dehalobacter sp. Coupling of two physiologically-distinct anaerobic consortia, aromatic ring degrader and reductive dechlorinator, resulted in the mineralization of pentachlorophenol under anaerobic conditions. These results suggested that gley paddy soils harbored anaerobic microbial community with versatile capacity degrading aromatic compounds under anaerobic conditions.

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Comparison of Anaerobic Digestion Efficiency with Different Temperature of Food Wastes (음식물류폐기물의 성상별 온도변화에 따른 혐기성소화 효율 비교 연구)

  • Hwang, Kwanghyun;Kim, Dongik
    • Journal of Korean Society on Water Environment
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    • v.35 no.4
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    • pp.332-339
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    • 2019
  • A comparative study on the anaerobic digestion efficiency according to the temperature change was conducted considering the characteristics of domestic food wastes with high water content of about 80 % or more. The substrate was tested for anaerobic digestion efficiency in two substrates, a liquid component separated naturally from food waste and food waste itself. In the anaerobic digestion experiments, the digestion efficiency was the highest at $55^{\circ}C$ (thermophilic temperature). However, the digestion efficiency at $45^{\circ}C$(middle high temperature) was lower than that at $35^{\circ}C$(mesophilic temperature). The comparison of general food wastes anaerobic digestion requiring 30 days of hydraulic retention time to the liquid component indicated a stable digestion efficiency even after 15 days of hydraulic retention time.In the experiments conducted on food waste, the digestion efficiency at $55^{\circ}C$ was higher than that at $35^{\circ}C$. When the food waste, especially the liquid component originating from food waste, is treated by anaerobic digestion method, the mesophilic temperature and thermophilic temperature conditions are more favorable in the digestion efficiency than the middle high temperature ($45^{\circ}C$). However, when applying thermophilic or mesophilic temperature anaerobic digestion process operation in the field, the amount of energy input should be considered.

Gender differences of anaerobic capabilities in untrained adults (비훈련 성인남여의 무산소성 운동능력 차이)

  • Jeong, Jinwon
    • Journal of Digital Convergence
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    • v.16 no.5
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    • pp.463-468
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    • 2018
  • The purpose of the study was to investigate the gender differences of anaerobic capabilities between anaerobic capacity(AC) from Wingate test and anaerobic work capacity(AWC) from critical power test in untrained male and female adults. Both tests were carried out to 12 male and 13 female subjects on a Monark cycle ergometer. The results of this study demonstrated that men were higher than women in AC for the Wingate test, but no gender difference(J/kg) in AWC for the Critical Power test. There was a significant relationship between AC(J/kg) and AWC(J/kg) in women(r=0.61, p<0.05), but no significant relationship in men(r=-0.32, p>0.05). ANCOVA analyses using $VO_{2max}$ and body weight as covariates had significant influence on the AWC gender difference. The study provides preliminary data on gender differences of anaerobic capabilities.

Waste Activated Sludge for Start-up Seed of Thermophilic Anaerobic Digestion (고온 혐기성 소화공정의 start-up seed로서의 호기성 폐 활성슬러지 이용가능성 연구)

  • Kim, Moonil;Shin, Kyuchul
    • Journal of Korean Society on Water Environment
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    • v.21 no.5
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    • pp.490-495
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    • 2005
  • Since there are very limited numbers of thermophilic anaerobic digesters being operated, it is often difficult to start up a new one using sludge from an existing reactor as a seed. However, for obvious reasons it seems few attempts have been made to compare the start-up performance of thermophilic anaerobic digestion using different sources of seed sludges. The purpose of this study was to evaluate the start-up performance of anaerobic digestion using aerobic Waste Activated Sludge (WAS) from a plant and mesophilic Anaerobic Digested Sludge (ADS) as the seed source at both mesophilic ($35^{\circ}C$) and thermophilic ($55^{\circ}C$) temperatures. In this study, two experiments were conducted. First, thermophilic anaerobic reactors were seeded with WAS (VSS = 4,400 mg/L) and ADS (VSS = 14,500 mg/L) to investigate start-up performance with a feed of acetate as well as propionate. The results show that WAS started to produce $CH_4$ soon after acetate feeding without a lag time, while ADS had a lag time of 10 days. When the feed was changed to propionate, WAS removed propionate down to below the detection limit of 10 mg/L, while ADS removed little propionate and produced little $CH_4$. Second, in order to further compare the methanogenic activity of WAS and ADS, both mesophilic and thermophilic reactors were operated. WAS acclimated to anaerobic conditions shortly and after acclimating it produced more $CH_4$ than ADS. WAS at mesophilic temperature biodegraded acetate at the same rate as for thermophilic. However WAS at mesophilic temperature biodegraded propionate at a much faster rate than at thermophilic. WAS as the seed source of anaerobic digestion resulted in much better performance than ADS at both mesophilic and thermophilic temperatures for both acetate and propionate metabolism.

Performances of Ceramic-tube and Pall-ring Upflow Anaerobic Filters Treating a Dairy Waste (세라믹튜브 및 패킹형플라스틱 여재충전 상향류식 혐기성여상에 의한 유가공 폐수처리)

  • Hur, Joon-Moo;Chang, Duk;Pae, Hyung-Suk;Kim, Soo-Young
    • Journal of Korean Society of Water and Wastewater
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    • v.14 no.1
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    • pp.37-44
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    • 2000
  • Laboratory experiments were conducted to investigate the performances of anaerobic filters packed with ceramic tube and pall-ring media treating a dairy waste. The media packing volume was 65% of effective volume of anaerobic filter. Organics removals of anaerobic filters were maintained above 80% even at an organics loading rate of $10kgCOD/m^3/d$, and this was comparable to aerobic treatment of organic wastes. Organics removals of the ceramic tube anaerobic filters were always lower than those of the pall-ring anaerobic filters due to intrinsic physical property of ceramic tube, especially lower void space which caused to clogging and entrapment of biogas, substrate transfer limitation, and irregular evolution of biogas leading to loss of solids and biomass. This was clearly observed in higher concentration of TSS in the effluent from the ceramic tube anaerobic filter despite of higher retention capacity of TSS compared with pall-ring media. Vertical distribution of organics and solids in the filters showed above 90% of organics and solids in influent were removed below 20% of reactor height, and 50% of remaining organics and solids were removed though media packing zone. Effluent quality from the anaerobic filter was heavily depended on media itself as well as suspended biomass formed below media. It is therefore concluded that the type of media played an important role in biomass accumulation arid gas-liquid-solid separation efficiency. Type of media did not affect the start-up behaviors of the anaerobic filter, and supernatant from anaerobic digested sludge showed a good performance as a seeding materials.

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Preliminary Evaluation of Leachate Recirculation Anaerobic Digestion System to treat Source Separated Food Waste (침출수 순환형 음식물류 폐기물 혐기성 소화공법에 대한 초기 특성 파악)

  • Lee, Byonghi;Lee, Jeseung
    • Journal of the Korea Organic Resources Recycling Association
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    • v.21 no.4
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    • pp.50-61
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    • 2013
  • In order to generate a renewable energy-Methane, anaerobic systems fed with source separated food waste from university cafeteria were studied. At first, four reactors were evaluated with same feed components; content non-mixing anaerobic reactor without leachate withdrawal/recirculation, content mixing anaerobic reactor without leachate withdrawal/recirculation, content non-mixing anaerobic reactor with leachate withdrawal/recirculation and content mixing anaerobic reactor with leachate withdrawal/recirculation. From the first study, content non-mixing anaerobic reactor with leachate withdrawal/recirculation showed the highest gas production. From further study with this system, it was observed that leachate permeation rate within anaerobic reactor was very important factor for gas generation. The higher permeation rate, the more gas production was observed. It is assumed that 1kg of gas collector weight and C/N ration above 10 in food waste may cause gas consumption in the anaerobic reactor. The gas consumption was estimated by negative pressure build-up at gas collector. The negative pressure build-up must be explained to produce Methane from Food Waste.

Monitoring of the Treatment of Leachate by Two Phase Anaerobic Membrane Process (TPAMP) (이상 혐기성 막공정에 의한 침출수 처리 모니터링)

  • Hwang, Moon H.;Hyun, Seong H.;Jang, Nam J.;Cho, Jea W.;Kim, In S.
    • Journal of Korean Society on Water Environment
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    • v.18 no.2
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    • pp.151-157
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    • 2002
  • Landfill leachate is one of highly contaminated and heterogeneous wastewater. The leachate from initial landfill can be treated by anaerobic process because it contains biodegradable matters, particularly, volatile fatty acids (VFAs). However, the anaerobic treatment of leachate is generally required longer hydraulic retention time (HRT) than aerobic process and another treatment process to satisfy effluent concentration. Therefore the modification of conventional anaerobic treatment is needed. Two phase anaerobic membrane process (TPAMP) is an integrated membrane process to be able to separate anaerobic metabolism into two phase which are acidogenesis and methanogenesis for improvement of anaerobic treatment efficiency. In this study, the efficiency of TPAMP and conventional anaerobic treatment were compared in terms of HRT, effluent SCOD, VFAs Membrane used in TPAMP was the UF of capillary type with the surface area of $0.048m^2$. The average effluent SCOD of conventional anaerobic treatment was 1352 mg/L and the removal was 96 % at HRT 60 days, while in TPAMP, 927 mg/L and 98% at HRT 30 days.

Correlation between Uni-standing Balance Ability, Jumping Ability, and Anaerobic Lower Extremity Muscle Power of High School Taekwondo Players

  • Jeong, Mo-beom
    • Physical Therapy Rehabilitation Science
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    • v.10 no.4
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    • pp.457-462
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    • 2021
  • Objective: This study analyzed the correlation between uni-standing balance ability, jumping ability, and anaerobic lower extremity power of high school Taekwondo players. Design: Cross-sectional study Methods: Two high schools recruited 36 taekwondo players, but 5 were dropped due to personal circumstances and preparations for the tournament, so the number was counted as 31. In order to test uni-standing balance ability, the Romberg test was performed using Biorescue to measure the sway area. In order to test jumping ability, standing long jump and standing highjump were measured. For anaerobic lower extremity muscle power test, the peak power and average power were measured through 30-second pedaling using a Wingate system bicycle. Results: Participants did not show any correlation with other measures in the uni-standing balance ability test (p>0.05). Looking at the correlation in the standing long jump, standing high jump (r=0.892, p<0.05), anaerobic peak power (r=0.698, p<0.05), anaerobic average leg power (r=0.662, p<0.05) showed a positive correlation. Looking at the correlations in the standing high jump, anaerobic peak power (r=0.663, p<0.05), anaerobic average power (r=0.697, p<0.05) showed a positive correlation. Looking at the correlation in the anaerobic peak power, average power (r=0.785, p<0.05) showed a positive correlation. Conclusions: This study shows that balance ability and anaerobic leg strength are independent of each other. The results of this study can be used as basic data for the training plan of high school taekwondo players in the future.