• Asian Pacific Journal of Cancer Prevention
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• v.14 no.3
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• pp.1655-1659
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• 2013

Background: HER-2/neu is a proto-oncogene that encodes a transmembrane tyrosine kinase growth factor which is crucial for stimulating growth and cellular motility. Overexpression of HER-2/neu is observed in 10-35% of human breast cancers and is associated with pathogenesis, prognosis as well as response to therapy. Given the imperative role of HER-2/neu overexpression in breast cancer, it is important to determine the magnitude of amplification which may facilitate a better prognosis as well as personalized therapy in affected patients. In this study, we determined HER-2/neu protein expression by immunohistochemistry (IHC) concurrently with HER-2/neu DNA amplification by quantitative real time-polymerase chain reaction (Q-PCR). Materials and Methods: A total of 53 paired tissue samples from breast cancer patients were frozen-sectioned to characterize the tumour and normal tissues. Only tissues with 80% tumour cells were used in this study. For confirmation, Q-PCR was used to determine the HER-2/neu DNA amplification. Results: We found 20/53 (37.7%) of the tumour tissues to be positive for HER-2/neu protein overexpression using IHC. Out of these twenty, only 9/53 (17%) cases were in agreement with the Q-PCR results. The concordance rate between IHC and Q-PCR was 79.3%. Approximately 20.7% of positive IHC cases showed no HER-2/neu gene amplification using Q-PCR. Conclusion: In conclusion, IHC can be used as an initial screening method for detection of the HER-2/neu protein overexpression. Techniques such as Q-PCR should be employed to verify the IHC results for uncertain cases as well as determination of HER-2/neu gene amplification.

• Information Systems Review
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• v.22 no.4
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• pp.163-184
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• 2020

The information on SNS, which is created by the forms of postings and comments, is being magnified and redistributed to news media expanding its impacts on real words. This amplifying effects of SNS comments have been increasingly discussed but there still lacks the answers for which dimensions of information is magnified, and what affects the direction and the degree of the amplification. This study, therefore, explores the detailed dimensions that are magnified by SNS comments and how SNS posting structure and social network characteristics affect them by using sentiment analysis. By analyzing 2,378 Facebook postings and news articles and their 26,312 SNS and 74,730 news site comments, this study shows that SNS comments magnify the sentiments of the posting articles they are attached to. In comparison to news site comments, SNS comments magnify the cognitive and social dimensions more than the news site comments. In the affective dimension, they tend to magnify only the positive emotion more than news site comments. Also, the findings reveal that whether the article in the posting is written by the posting owner affects the degree of amplification when the comments are remained positive or switched positive, while the opposite determines the amplification when comments remain negatively, suggesting that the user relationship in social network is the important factor that affects the direction and the degree of the information amplification in SNS.

• Journal of the Optical Society of Korea
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• v.16 no.3
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• pp.277-281
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• 2012

We measured small optical beam deflection in an image rotating Sagnac interferometer. We used a weak value measurement scheme that involves a pre-selection, weak perturbation, and a post-selection procedure to obtain the amplified value of beam deflection. The amplification factor of the measured beam deflection varied from 11 to 63 depending on the settings of the post-selection polarizer in front of the photodetector and the settings of polarization compensator in the interferometer.

• Journal of applied mathematics & informatics
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• v.41 no.1
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• pp.155-165
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• 2023

We consider circular Rayleigh problem of hydrodynamic stability which deals with linear stability of axial flows of an incompressible iniviscid homogeneous fluid to axisymmetric disturbances. For this problem, we obtained two parabolic instability regions which intersect with Batchelor and Gill semi-circle under some condition. This has been illustrated with examples. Also, we derived upper bound for the amplification factor.

• Journal of applied mathematics & informatics
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• v.41 no.2
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• pp.363-372
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• 2023

We consider Kuo problem of hydrodynamic stability which deals with incompressible, inviscid, parallel shear flows in the 𝛽-plane. For this problem, we derived instability region without any approximations and which intersects with Howard semi-circle region under certain condition. Also, we derived upper bound for growth rate and amplification factor of an unstable mode and proved Howard's conjecture.

• Journal of applied mathematics & informatics
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• v.41 no.5
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• pp.1071-1084
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• 2023

We consider inviscid, incompressible homogeneous shear flows of variable cross section known as extended Rayleigh problem. For this extended Rayleigh problem, we derived instability region which intersect with semi-circle instability region under some condition. Also we derived condition for stability, upper bound for amplification factor and growth rate of an unstable mode.

• Journal of Gastric Cancer
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• v.16 no.3
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• pp.141-151
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• 2016

Purpose: The aim of the present study was to elucidate the clinicopathological significance and diagnostic accuracy of immunohistochemistry (IHC) for determining the mesenchymal epidermal transition (c-MET) expression in patients with gastric cancer (GC). Materials and Methods: The present meta-analysis investigated the correlation between c-MET expression as determined by IHC and the clinicopathological parameters in 8,395 GC patients from 37 studies that satisfied the eligibility criteria. In addition, a concordance analysis was performed between c-MET expression as determined by IHC and c-MET amplification, and the diagnostic test accuracy was reviewed. Results: The estimated rate of c-MET overexpression was 0.403 (95% confidence interval [CI], 0.327~0.484) and it was significantly correlated with male patients, poor differentiation, lymph node metastasis, higher TNM stage, and human epidermal growth factor receptor 2 (HER2) positivity in IHC analysis. There was a significant correlation between c-MET expression and worse overall survival rate (hazard ratio, 1.588; 95% CI, 1.266~1.992). The concordance rates between c-MET expression and c-MET amplification were 0.967 (95% CI, 0.916~0.987) and 0.270 (95% CI, 0.173~0.395) for cases with non-overexpressed and overexpressed c-MET, respectively. In the diagnostic test accuracy review, the pooled sensitivity and specificity were 0.56 (95% CI, 0.50~0.63) and 0.79 (95% CI, 0.77~0.81), respectively. Conclusions: The c-MET overexpression as determined by IHC was significantly correlated with aggressive tumor behavior and positive IHC status for HER2 in patients with GC. In addition, the c-MET expression status could be useful in the screening of c-MET amplification in patients with GC.

• International Journal of Precision Engineering and Manufacturing
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• v.8 no.2
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• pp.70-74
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• 2007

This paper describes the development of a nano-positioning system for nanoscale science and engineering. Conventional positioning systems, which can be expensive and complicated, require the use of laser interferometers or capacitive transducers to measure nanoscale displacements of the stage. In this study, a new self-displacement sensing (SDS) nano-stage was developed using mechanical magnification of its displacement signal. The SDS nano-stage measured the displacement of its movement using a position-sensitive photodiode (PSPD), a laser source, and a hinge-connected rotating mirror plate. A beam from a laser diode was focused onto the middle of the plate with the rotating mirror. The position variation of the reflected beam from the mirror rotation was then monitored by the PSPD. Finally, the PSPD measured the amplified displacement as opposed to the actual movement of the stage via an optical lever mechanism, providing the ability to more precisely control the nanoscale stage. The displacement amplification process was modeled by structural analysis. The simulation results of the amplification ratio showed that the distance variation between the PSPD and the mirror plate as well as the length L of the mirror plate could be used as the basic design parameters for a SDS nano-stage. The PSPD was originally designed for a total travel range of 30 to 60 mm, and the SDS nano-stage amplified that range by a factor of 15 to 25. Based on these results, a SDS nano-stage was fabricated using principle of displacement amplification.

• Journal of The Korean Astronomical Society
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• v.31 no.1
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• pp.27-37
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• 1998

We have made semi-analytical studies to investigate the configurations of caustics and the probability distribution of the flux factor K for the binary microlensing including external shears. A parametric equation of critical curve is derived in a 4th order complex polynomial. We present the topological dependencies of the caustics for selected gamma parameters (0, 0.3, 0.6, 1.3, 2.0, and 2.5) and convergence terms (0., 0.4, 0.8, 1.2, 1.6, and 2.0). For the purpose of analyzing the efficiency of High Amplification Event (HAE) on each caustics, we examine the probability distribution of the flux factor by a Monte Carlo method. Changing the separation of the binary system from 0.8 to 1.3 (in normalied unit), we examine the probability distribution of the K-values in various gamma parameters. The relationship between gamma parameters, seperations and their probabilties of the flux factor K have been studied. Our results show that the relatively higher K values (K>1.5) are increased as increasing the separation of the binary system. We therfore conclude that, in the N-body microlensing, the probabilities of higher HAEs are inversely proportional to the star density as well. We also point out that the present research might be used as a preliminary step toward investigating heavy N-body microlensing simulations.

• Korean Journal of Pharmacognosy
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• v.48 no.3
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• pp.173-178
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• 2017

We investigated the efficacy of single compound of plant extract in coxsackievirus B3 (CVB3) infection. CVB3 is a main cause of Hand-foot-mouth diseases (HFMD) and viral myocarditis in children and adult. Several single compounds of plant extract were purified by HPLC and tested as antiviral drug candidate. Among them, kaempferol was selected to effective anti-enterovirus compound by HeLa cells survival assay. CVB3 infected HeLa cells were treated with kaempferol ($100{\mu}g/ml-100ng/ml$) and their antiviral effect was confirmed. After 16 hours of treatment, HeLa cells were lysed and proteins were extracted for western blot analysis. CVB3 viral capsid protein VP1 production and transcription factor eIF4G-1 cleavage was significantly decreased in $100{\mu}g/ml$ kaempferol treatment. Virus replication was observed by virus RNA amplification. Kaempferol strongly reduced virus positive and negative strand RNA amplification. Moreover, MAPK signal induced by CVB3 infection, pERK and pmTOR, kaempferol treatment significantly inhibited the activity. Plant extract single compound, kaempferol, is a strong candidate to be developed non-toxic anti-enterovirus treatment agent.