• Title/Summary/Keyword: ammonium sulfate

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Simple Purification of Bromelain from Pineapple

  • Ko, Bo-Sung;Hwang, Yong-Il;Lee, Seung-Cheol
    • Preventive Nutrition and Food Science
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    • v.1 no.1
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    • pp.106-110
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    • 1996
  • Bromelain(EC 3.4.22.4), the collective name for the proteolytic enzymes found in tissues of the plant family Bromeliaceae(pineapple), has been used as a tenderizing agent in food processing, and as an antiinflammatory agent in pharmaceuticals. In this paper, we describe the simple purification method of bromelain using Korean pineapple fruit. After removing contaminants at 30% saturation of ammonium sulfate, the supernatant obtained was treated again with ammonium sulfate to 80% saturation. Wit the above salt fractionation, partially purified bromelain could be obtained. To get highly purified bromelain, the previous 30% to 80% ammonium sulfate treated precipitate was dialyzed against 25mM sodium acetate buffer(pH 5.0) followed by passing through a CM- cellulose cation exchange column. Fruit bromelain was eluted as a major peak at 0.5~0.8M NaCI gradient. The present method is simpler with high wield than the traditional purification method-acetone treatment and several consecutive chromatographic processes.

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Urea fertilizing effect on mulberry tree (상수에 대한 요소 비효시험)

  • 박병희;노현상
    • Journal of Sericultural and Entomological Science
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    • v.3
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    • pp.69-72
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    • 1963
  • The purpose of this work is to investigate the effects of urea and ammonium sulfate on Kairyo Nezumigaeshi mulberry from 1959 to 1963 at Sericultural Experiment Station. The results obtained are as follows. 1. Both urea and ammonium sulfate were the same fertilizing effects. 2. The optimum fertilizer ratio of urea and ammonium sulfate for spring and summer fertilizing was 40 : 60 in case of mulberry pot culture. This was good for harvest.

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Production and Purification of Alkaline Protease from Streptomyces sp. (Streptomyces속 균주가 생성하는 Alkaline Protease의 생산 및 정제)

  • Choi, Cheong;Chung, Yung-Gun;Sung, Sam-Kyung;Choi, Kwang-Soo;Lee, Jae-Sung;Cho, Young-Je;Kwon, Oh-Jin
    • Microbiology and Biotechnology Letters
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    • v.20 no.2
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    • pp.169-177
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    • 1992
  • An alkaline protease producing microorganism was isolated from soil and identified as Streptomyces griseus HC-1141. The optimum culture condition of Streptomyces griseus HC- 1141 for the production of alkaline protease was as follows; 0.5% casein, 0.05% ammonium chloride, 0.1% ferrous sulfate. 2.0% lactose, pH 8.0 and 84 hrs. The enzyme was purified about 53 folds by ammonium sulfate treatment, DEAE-cellulose ion exchange chromatography and gel filtratioo on Sephadex G-150. The homogeneity of the purified enzyme was verified by polyacrylamide gel electrophoresis. The molecular weight was estimated to be 31,000 on sodium dodecyl sulfate polyacrylamide gel electrophoresis. This enzyme consists of glycine and glutamic acid as major amino acids. The N-terminal and C-terminal residues of the alkaline protease were leucine and histidine respectively.

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Effect of Bonding on Bending and Shear Strength of COMPLY Composed of Fire Retardant Treated Particleboard (내화처리(耐火處理) 파티클보오드의 COMPLY접착(接着)이 휨강도(強度)와 전단강도(剪斷強度)에 미치는 영향(影響))

  • Lee, Phil Woo;Kwon, Jin Heon
    • Journal of Korean Society of Forest Science
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    • v.64 no.1
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    • pp.26-32
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    • 1984
  • This research was conducted to examine the effect of bonding on the bending and shear strength of COMPLY-board when used with meranti face veneer and fire retardant treated particle-board core. For ammonium sulfate, modulus of rupture (MOR) of COMPLY-board increased with increasing chemical concentration up to 10 percent and then decreased after that concentration. However, Minalith showed reverse effect to the result for ammonium sulfate. Modulus of elasticity (MOE) of COMPLY-hoard decreased with increasing chemical concentration and its differences were not significant between concentration. Shear strength was shown better when treated with ammonium sulfate than with Minalith. Wood failure of COMPLY increased with increasing chemical concentration up to 15 percent and then decreased after that concentration. The products of parallel and cross veneer laminated were better than those of COMPLY composed of fire retardant treated particle-board core. Non-treated particle-board was the least in strength. MOE of fire retardant treated COMPLY increased by 136 percent and its MOE by 170 percent as compared with non treated particle-board.

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Effect of Nitrogen Sources on Mycelial Growth and Sclerotial Formation of Rhizoctonia solani Causing Rice Sheath Blight (벼 잎집무늬마름병균의 균사생장 및 균핵형성에 미치는 질소원의 효과)

  • Park Kyung Seok;Chung Bong Koo
    • Korean Journal Plant Pathology
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    • v.1 no.1
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    • pp.44-50
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    • 1985
  • Effect of nitrogen sources on mycelial growth and sclerotial formation of Rhizoctonia solani causing rice sheath blight was studied by using sixty isolates from diseased samples of different localities in Chungbuk area. Based on the cultural characters, pathogenicity and sclerotial formation, three isolates of the fungus were selected. The virulence of the three isolates varied based on the percent of seeds germination, seedling blights and length and lesion number of lesions. As nitrogen sources, arginine, alanine, urea, ammonium sulfate and sodium nitrate were more effective for mycelial growth and sclerotial formation, than were cystine, methionine, lysine, histidine, tryptophan and thyrosine. The mycelium of R. so/ani grew well in a lower concentration of urea, sodium nitrate, arginine, ammonium sulfate and lysine, but in a higher concentration of proline, histidine, and alanine. In all nitrogen sources tested, high concentration of nitrogen nutrients greatly stimulated sclerotial formation of the fungus. Arginine, urea, and ammonium sulfate increased virulence of the fungus. All the isolates grown on the basic medium plus proline produced large sclerotia. Mycelial growth of the fungus was not well in the tryptophan-added medium, showing brown discoloration of medium.

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Effects of Nitrogen and Potassium Sources on Growth and Yield of Strawberry 'Seolhyang' and 'Maehyang' in Fertigation Culture (딸기 '설향' 및 '매향'의 관비재배 시 질소 및 칼리의 공급원이 생육 및 수량에 미치는 영향)

  • Kim, Seung-Yu;Jang, Yoon-Ah;Moon, Ji-Hye;Lee, Jun-Gu;Lee, Sang-Gyu;Cha, Seon-Hwa
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.436-441
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    • 2009
  • The experiment was conducted to investigate the effect of nitrogen and potassium sources on growth and yield of strawberry 'Seolhyang' and 'Maehyang' in fertigation culture. The plant fresh and dry weight were higher in urea + potassium sulfate and ammonium sulfate + potassium sulfate as nitrogen and potassium sources than others in both varieties. But there were no significant difference among nitrogen and potassium sources in other growth characteristics, such as plant height, no. of leaf, crown diameter etc. Also, the marketable yield of fruit were higher in urea + potassium sulfate and ammonium sulfate + potassium sulfate in 'Seolhyang' than other treatments. 'Maehyang' was better in both treatments but there was no significant difference. The fruit qualities, such as total soluble solid, hardness, acidity, vitamin C content were not significant difference among the treatments. The acidity of soil was tended to decrease but EC was tended to increase highly in ammonium sulfate treatments as a nitrogen source. As above results, the urea and potassium sulfate were recommended as nitrogen and potassium sources for the fertigation culture of strawberry 'Seolhyang' and 'Maehyang'.

Effect of Treatments with Flame-retardant on Flame-resistance and Tensile Strength of Paper (난연 처리가 종이의 난연성 및 인장강도에 미치는 영향)

  • Song, Han-Kyu;Lee, Myoung-Ku
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.38 no.4 s.117
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    • pp.61-67
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    • 2006
  • The effect of several inorganic flame-retardants such as ammonium phosphate, ammonium sulfate, aluminum hydroxide and antimony trioxide on the flame-retardant property and tensile strength of paper has been investigated. Flame-retardants were used preferably as a dry powdered mixture and added to the furnish. Both dipping and coating treatments were employed to apply flame-retardants to paper Flame-retardant paper was manufactured by treatment of $5{\sim}30%$ flame-retardants by weight of the paper on a dry weight. Paper's flame-retardant property and tensile strength were examined by comparison of char length and tensile index. As dosages of flame-retardant chemicals increased, flame-retardant property was improved but tensile index was decreased.

Purification of Festriction Endonuclease,SdiI, from Streptomyces diastatochromogenes (Streptomyces diastatochromogens로부터 제한효소 SdiI의 분리정제)

  • Bae, Mu;Song, Eun-Suk
    • Korean Journal of Microbiology
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    • v.32 no.4
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    • pp.297-300
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    • 1994
  • About thirty bacterial strains of actinomycete isolated from the soil were examined for the presence of restriction endonuclease activity. Streptomyces diastatochromogenes, which was identified previously, was found to contain restriction endonuclease activity. The purification of this enzyme, SdiI, was carried out via streptomycin sulfate precipitation and ammonium sulfate fractionation followed by hydroxylapatite column chromatography. Sephacryl S-200 HR column chromatography and second hydroxylapatite column chromatography. SDS-polyacrylamide gel electrophoresis of the active protein (purified from various column chromatography) resulted in 35,000 Da protein.

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Atmospheric Nitrate, sulfate, Ammonium, and Calcium Concentrations at Forest Area in Jeiu Island (제주도 산림지역에서의 대기중 Nitrate, Sulfate, Ammonium, Calcium 농도 분석)

  • 김대준;강창희;허철구;이기호
    • Proceedings of the Korea Air Pollution Research Association Conference
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    • 2003.11a
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    • pp.151-152
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    • 2003
  • 대기중에는 토양입자나 해양입자와 같이 직접적으로 방출되는 입자상 물질 이외에 자연적으로 생성되거나 인간의 활동에 의하여 배출되는 유황, 질소산화물 및 유기물의 가스상 물질이 대기 화학 반응에 의하여 입자상 물질로 변환된 이차입자가 혼재되어 있다. 이러한 입자상물질은 $K^{+}$, $Na^{+}$, $Mg^{2+}$, NH$_4$$^{+}$, $Ca^{2+}$ 등의 양이온과 Cl$^{-}$, NO$_3$$^{-}$, SO$_4$$^{2-}$ 등의 음이온 성분으로 구성되어 있다. (중략)

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