• Title/Summary/Keyword: amino-polysaccharide

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GCP (GENISTEIN CONCENTRATED POLYSACCHARIDE): A SOYBEAN ISOFLAVONE DIETARY SUPPLEMENT FERMENTED BY BASIDIOMYCETES WITH ANTI CARCINOGENIC ACTIVITIES: THEIR MOLECULAR MECHANISMS AND CHEMOPREVENTIVE POTENTIAL

  • Lan Yuan;Takehito Miura;Mayumi Yoshida;Chihiro Wagatsuma;Hajime Fujii;Tomomi Mukoda;Sun, Bu-Xiang;Kenichi Kosuna
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2001.10a
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    • pp.15-16
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    • 2001
  • GCP is a novel functional food obtained from the extracts of soybean isoflavone fermented with basidiomycetes mycelia. The enzyme reaction makes GCP containing high concentration of isoflavone aglycons, particularly for genistein and high content of polysaccharides. GCP exerts its anti-tumor activities by anti-angiogenesis and induction of apoptosis in cancer tissues and by enhancement of immune responses in immune system.(omitted)

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Simultaneous Enantiomer Separation of α-Amino Acids and Their Esters as Fluorenylmethoxycarbonyl Derivatives under UV and Fluorescence Detection by High Performance Liquid Chromatography (고성능 액체 크로마토그래피에서 아미노산과 이들 에스테르의 플루오레닐메톡시카르보닐 유도체의 자외선과 형광 검출에서의 동시 광학분리)

  • Islam, Md. Fokhrul;Lee, Wonjae
    • KSBB Journal
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    • v.30 no.4
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    • pp.197-201
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    • 2015
  • Liquid chromatographic enantiomer separation of ${\alpha}$-amino acids and their methyl and ethyl esters as fluorenylmethoxycarbonyl (FMOC) derivatives was performed using a recently developed chiral column (Chiralpak IE) based on polysaccharide derivative under simultaneous UV detection and fluorescence detection. The degree of enantiomer separation of ${\alpha}$-amino acid esters as FMOC derivatives is generally higher than that of the corresponding ${\alpha}$-amino acids. Especially, ${\alpha}$-amino acid methyl esters showed the greatest enantioseparation. As this method developed in this study can be applied to determine the chemical and optical purity of ${\alpha}$-amino acids and esters, it is expected to be quite useful for their chiral separation using Chiralpak IE.

Compositional Analysis of Polysaccharide from Sanguisorba officinalis and Its Anticoagulant Activity (지유로부터 분리한 다당류의 분석과 항응고작용)

  • Kim, Yeong-Shik;Roh, Ji-Eun;Ann, Hyung-Soo
    • Korean Journal of Pharmacognosy
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    • v.24 no.2
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    • pp.124-130
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    • 1993
  • Polysaccharide from Sanguisorba officinalis was separated and fractionated using DEAE-Sephadex ion-exchange chromatography and Sephacry HR-200 gel filtration chromatography. One of the fraction(Fr. II) was sulfated and its anticoagulant activity was tested in vitro. Sulfation could increase the clotting time 50 times compared to unsulfated one. Fr. II was hydrolyzed and its composition was analyzed by conjugation with 7-amino-1, 3-naphthalene disulfonic acid using HPLC and electrophoresis. Arabinose and galactose were mainly composed at the ratio of 4 : 1. In addition, xylose and rhamnose were also found.

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Antitumor Effect of Polysaccharide Produced from a Mutant of Acetobacter pasteurianus IFO 13751-5 (Acetobacter pasteurianus IFO 13751-5 변이주가 생산하는 다당류의 항암효과)

  • Kim, Dong-Seuk;Ryu, Beung-Ho
    • Korean Journal of Food Science and Technology
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    • v.23 no.4
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    • pp.405-409
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    • 1991
  • The polysaccharide was predominantly composed of glucose (79.60%), galactose (9.68%), mannose(8.67%), fructose (1.96%;) and xylose (0.09%). Major amino acid in protein was consisted of cystein, aspartic acid, valine and glutamic acid. The tumor growth inhibition ratio of the polysaccharide against Sarcoma 180 was at highest level of 64.96%, when administerd at the concentration of 50 mg/kg and life prologation ratio showed 28.91% at the concentration of 50 mg/kg. The direct cytotoxic effect of polysaccharide was not observed in the tumor bearing mice with Sarcoma 180 in vitro.

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The Effect of Mobile Phase and Dissolving Solvent on the Enantiomer Separation Using a Covalently Immobilized Chiral Column Derived from Polysaccharide Derivative (다당유도체로 공유결합된 카이랄 칼럼에서 이동상과 분석물질의 용매가 거울상 이성질체의 광학분할에 미치는 영향)

  • Huang, Hu;Lee, Beom-Gyu;Baek, Chae-Sun;Lee, Won-Jae
    • Journal of the Korean Chemical Society
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    • v.53 no.2
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    • pp.137-143
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    • 2009
  • Liquid chromatographic enantiomer separation of N-tert-butoxycarbonyl (BOC) $\alpha$-amino acids and their ethyl esters was performed on covalently immobilized chiral column (Chiralpak IC) derived from polysaccharide derivative. The solvent versatility of the covalently immobilized Chiralpak IC in enantiomer separation of N-BOC $\alpha$-amino acid ethyl ester derivatives was shown and the chromatographic parameters of their enantioselectivities and resolution factors were greatly influenced by the nature of the mobile phase. Also the effect on the dissolving solvent for these analytes on the enantiomer separation using the same mobile phase and the examples of preparative enantiomer separation on analytical column were shown.

Effect of acid or base additive in the mobile phase on enantiomer separation of amino alcohols using polysaccharide derived chiral columns (다당유도체를 기초로 한 키랄 컬럼에서 이동상에서의 산 또는 염기 첨가제가 아미노 알코올의 광학분리에 미치는 영향)

  • Huang, Hu;Jin, Jing Yu;Lee, Wonjae
    • Analytical Science and Technology
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    • v.22 no.4
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    • pp.313-318
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    • 2009
  • Liquid chromatographic enantiomer separation of amino alcohols was performed on several chiral columns based on polysaccharide derivatives under the mobile phase conditions containing acid or base additive. The chromatographic parameters were greatly influenced by the nature of the mobile phase containing acid or base additive as well as the used chiral columns. Compared to chromatographic results obtained in the mobile phase containing base additive (0.1% triethylamine), especially, Chiralcel OD showed dramatically enhanced separation factors and resolution factors with reduced capacity factors under the mobile phase condition containing acidic additive (0.1% trifluoroacetic acid). When the mobile phase containing 0.05% trifluoroacetic acid and 0.05% triethylamine was used on Chiralcel OD, the greatest separation factors and resolution factors among all other mobile phase conditions in this study were observed.

Liquid chromatographic enantioseparation of several amino acids as nitrobenzoxadiazole derivatives on polysaccharide trisphenylcarbamate derived chiral stationary phases

  • Suraj Adhikari;Alisha Bhandari;Wonjae Lee
    • Analytical Science and Technology
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    • v.36 no.4
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    • pp.143-151
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    • 2023
  • Considering the greater role of α-amino acids in our daily lives, the enantiomer resolution of seven α-amino acids derivatized with fluorogenic reagent (4-fluoro-7-nitro-2,1,3-benzoxadiazole, NBD-F) by chiral HPLC on amylose or cellulose trisphenylcarbamate derived chiral stationary phases (CSPs) under simultaneous ultraviolet (UV) and fluorescence (FL) detection was performed. The degree of enantioseparation and resolution was affected by nature and selector backbones of the CSPs as well as the kind of amino acids. Baseline enantiomer separation and resolutions were observed for the enantiomers of all analytes as NBD derivatives especially on coated type amylose tris(3,5-dimethylphenylcarbamate) derived CSPs (Chiralpak AD-H and Lux Amylose-1). The other CSPs also showed good enantioselectivity except for the CSPs (Chiralpak IB, Chiralcel OD-H and Lux Cellulose-1) having cellulose tris(3,5-dimethylphenylcarbamate) as chiral selectors. The developed analytical chiral method was applied to determine the enantiomeric purity of seven commercially available L-α-amino acids and the impurities as D-forms were found to be in the range 0.08-0.87 %, respectively. The intra- and interday accuracy and precision assays showed high accuracy and precision of the developed analytical method. This chiral HPLC method for the enantiomer resolution of amino acids using fluorescent derivatization could be useful for the determination of enantiomeric purity of pharmaceuticals and biological study for amino acid type compounds among chiral drugs.

Liquid Chromatographic Resolution of α-Amino Acid Esters as Benzophenone Imine Derivatives (아미노산 에스테르의 벤조피논 이민 유도체의 액체 크로마토그래피의 광학분리)

  • Yun, Won-Nam;Xu, Wen Jun;Huang, Hu;Lee, Won-Jae
    • KSBB Journal
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    • v.27 no.3
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    • pp.167-171
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    • 2012
  • A convenient liquid chromatographic enantiomer separation of several ${\alpha}$-amino acid esters as benzophenone Schiff base derivatives on covalently immobilized chiral stationary phases (CSPs) derived from polysaccharide derivatives was developed. The benzophenone imine derivatives of ${\alpha}$-amino acid esters were readily prepared by stirring benzophenone imine and the ${\alpha}$-amino acid ester hydrochloride salts in 2-propanol. The chromatographic conditions used on all CSPs were 0.5% or 5% 2-propanol/hexane (V/V) as the mobile phases at 1 mL/min of flow rate and UV 254 nm detection. The performance of Chiralpak IC among all CSPs was superior to that of the other CSPs for resolution of benzophenone imine derivatives of ${\alpha}$-amino acid esters. It is expected that the developed analytical method will be useful for enantiomer resolution of other ${\alpha}$-amino acid esters as benzophenone Schiff base derivatives.

Antitumor Activity of Protein-Plysacharides Produced form Vibrio anguillarum

  • Yu, Byeong-Ho;Chi, Boung-Ho;Kim, Dong-Suck;Jang, Mi-Kyung;Kim, Hae-Sung;Chung, Soo-Ja
    • Journal of Food Hygiene and Safety
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    • v.3 no.3
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    • pp.111-116
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    • 1988
  • The antitumor activity of protein-polysaccharide produced by a strain, Vibrio anguilfarum, No. 17 isolated from sea water was investigated. The extracellular protein,polysaccharide used in this experiment was obtained through the cultivation of Vibrio anguillarum No. 17 at $25^{\circ}C$ for 5-7 days in the sea water medium containing 0.5% peptone and 0.1% yeast extract. The compositional monosaccharides of protein-polysaccharide were xylose, mannose, galactose, glucose and fructose in order and its major amino acids were glutamic acid, serine and aspartic acid. The antitumor activity of the protein-polysaccharide at a dose of 0.5mg/kg/day or 5mg/kg/day against Sarcoma-180 in mice were 20.9% and 43.9%, respectively.

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Studies on Constituents of Higher Fungi of Korea(XXXV) -Antitumor Components Extracted from the Carpophores and Cultured Mycelia of Laccaria laccata- (한국산(韓國産) 고등(高等) 균류(菌類)의 성분(成分) 연구(硏究)(제(第)35보(報)) -애기졸각버섯의 항암(抗癌) 성분(成分)-)

  • Kim, Sook-Hee;Woo, Myoung-Sik;Kim, Byong-Kak
    • The Korean Journal of Mycology
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    • v.10 no.4
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    • pp.155-163
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    • 1982
  • To find antitumor components, a protein-bound polysaccharide fraction was obtained by extracting with hot water and precipitating with ethanol from the carpophores and the cultured mycelia of Laccaria laccata. The fraction was examined for antitumor activity against sarcoma 180 implanted in mice. The component extracted from the carpophores showed 75% and 65% tumor inhibition ratios in the doses of 20 mg and 50 mg/kg/day, respectively. The protein bound polysaccharide fraction of the cultured mycelia of L. laccata also showed 57.8% tumor inhibition ratio in the dose of 20 mg/kg/day. The chemical analysis of the protein-bound polysaccharide fraction showed that it contained a polysaccharide and a protein. The hydrolysates of the polysaccharide moiety contained six and one unknown monosaccharides. Fourteen amino acids were identified in the hydrolysates of the protein moiety.

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