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http://dx.doi.org/10.7841/ksbbj.2015.30.4.197

Simultaneous Enantiomer Separation of α-Amino Acids and Their Esters as Fluorenylmethoxycarbonyl Derivatives under UV and Fluorescence Detection by High Performance Liquid Chromatography  

Islam, Md. Fokhrul (College of Pharmacy, Chosun University)
Lee, Wonjae (College of Pharmacy, Chosun University)
Publication Information
KSBB Journal / v.30, no.4, 2015 , pp. 197-201 More about this Journal
Abstract
Liquid chromatographic enantiomer separation of ${\alpha}$-amino acids and their methyl and ethyl esters as fluorenylmethoxycarbonyl (FMOC) derivatives was performed using a recently developed chiral column (Chiralpak IE) based on polysaccharide derivative under simultaneous UV detection and fluorescence detection. The degree of enantiomer separation of ${\alpha}$-amino acid esters as FMOC derivatives is generally higher than that of the corresponding ${\alpha}$-amino acids. Especially, ${\alpha}$-amino acid methyl esters showed the greatest enantioseparation. As this method developed in this study can be applied to determine the chemical and optical purity of ${\alpha}$-amino acids and esters, it is expected to be quite useful for their chiral separation using Chiralpak IE.
Keywords
Amino acid derivative; Chiral column; Enantiomer separation; Fluorenylmethoxycarbonyl derivative;
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Times Cited By KSCI : 2  (Citation Analysis)
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