• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.1901-1906
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• 2012

Background: The discovery that microRNA (miRNA) regulates metastasis provide a principal molecular basis for tumor heterogeneity. A characteristic of solid tumors is their heterogenous distribution of blood vessels, with significant hypoxia occurring in regions (centers of tumor) of low blood flow. It is necessary to discover the mechanism of breast cancer metastasis in relation to the fact that there is a differential distribution of crucial microRNA in tumors from centers to edges. Methods: Breast tissues from 48 patients (32 patients with breast cancer) were classified into the high invasive and metastatic group (HIMG), low invasive and metastatic group (LIMG), and normal group. Samples were collected from both the centers and edges of all tumors. The first six specimens were detected by microRNA array, and the second ten specimens were detected by real-time qRT-PCR and Western blot analyses. Correlation analysis was performed between the miRNAs and target proteins. Results: The relative content of miR-20a and miR-20b was lower in the center of the tumor than at the edge in the LIMG, lower at the edge of the tumor than in the center in the HIMG, and lower in breast cancer tissues than in normal tissues. VEGF-A and HIF-1alpha mRNA levels were higher in the HIMG than in the LIMG, and levels were higher in both groups than in the normal group; there was no difference in mRNA levels between the edge and center of the tumor. VEGF-A and HIF-1alpha protein levels were higher in the HIMG than in the LIMG, and protein levels in both groups were higher than in the normal group; there was a significant difference in protein expression between the edge and center of the tumor. Correlation analysis showed that the key miRNAs (miR-20a and miR-20b) negatively correlated with the target proteins (VEGF-A and HIF-1alpha). Conclusions: Our data suggest that miR-20a and miR-20b are differentially distributed in breast cancer, while VEGF-A and HIF-1alpha mRNA had coincident distributions, and VEGF-A and HIF-1alpha proteins had uneven and opposing distributions to the miRNAs. It appears that one of the most important facets underlying metastatic heterogeneity is the differential distribution of miR-20a and miR-20b and their regulation of target proteins.

• IMMUNE NETWORK
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• v.2 no.4
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• pp.242-247
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• 2002

Background: Tumor necrosis factor-alpha (TNF-$\alpha$) is known to play an important role in various conditions such as inflammation, autoimmunity, apoptosis, insulin resistance and sleep induction. Five single nucleotide polymorphisms (SNPs) have been known to affect the transcriptional activities of TNF-$\alpha$: -1,031T/C, -863C/A, -857C/T, -308G/A and -238G/A. Methods: We have investigated 5 SNPs of the promoter region of TNF-$\alpha$ gene, the distribution of 5-locus TNF-$\alpha$ haplotypes, and their haplotypic associations with previously typed HLA-A, -B and -DRB1 loci in 107 healthy unrelated Koreans. TNF-$\alpha$ SNPs were typed using PCR-single-strand conformation polymorphism (SSCP) and PCR-restriction fragment length polymorphism (RFLP) methods. Results: The allele frequencies of -1,031C, -863A, -857T, -308A, and-238A, which are known as the high-producer-type, were 19.3%, 15.9%, 14.0%, 5.9%, and 2.9%, respectively. The frequency of -308A allele, known to be associated with autoimmune diseases, was 5.9% in Koreans which was lower than Caucasians (14~17%) and somewhat higher than Japanese (1.7%). Five most common TNF-$\alpha$ haplotypes (-1,031/-863/-857/-308/-238) comprised over 95% of total haplotypes: TCCGG (58.4%), CACGG (14.8%), TCTGG (13.7%), TCCAG (5.3%), and CCCGA (3.1%). Strong positive associations (P<0.001) were observed between TCCGG and B62; between CACGG and B51, $DRB1^*0901$; between TCTGG and B35, B54, B59, $DRB1^*1201$; and between TCCAG and A33, B58, $DRB1^*0301$, $DRB1^*1302$. Five most common extended haplotypes (>3%) comprised around 16% of total haplotypes: A33-B58-TCCAG-$DRB1^*1302$, A24-B52-TCCGG-$DRB1^*1502$, A33-B44-TCCGG-$DRB1^*1302$, A24-B7-TCCGG-$DRB1^*0101$, and A11-B62-TCCGG-$DRB1^*0406$. The distribution of extended HLA and TNF-$\alpha$ haplotypes showed that most of HLA haplotypes were almost exclusively associated with particular TNF-$\alpha$ haplotypes. Conclusion: The results obtained in this study would be useful as basic data for anthropologic studies and disease association studies in Koreans.

• Molecules and Cells
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• v.40 no.5
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• pp.355-362
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• 2017

The ${\beta}2$ integrins are cell surface transmembrane proteins regulating leukocyte functions, such as adhesion and migration. Two members of ${\beta}2$ integrin, ${\alpha}M{\beta}2$ and ${\alpha}X{\beta}2$, share the leukocyte distribution profile and integrin ${\alpha}X{\beta}2$ is involved in antigen presentation in dendritic cells and transendothelial migration of monocytes and macrophages to atherosclerotic lesions. ${\underline{R}}eceptor$ for ${\underline{a}}dvanced$ ${\underline{g}}lycation$ ${\underline{e}}nd$ ${\underline{p}}roducts$ (RAGE), a member of cell adhesion molecules, plays an important role in chronic inflammation and atherosclerosis. Although RAGE and ${\alpha}X{\beta}2$ play an important role in inflammatory response and the pathogenesis of atherosclerosis, the nature of their interaction and structure involved in the binding remain poorly defined. In this study, using I-domain as a ligand binding motif of ${\alpha}X{\beta}2$, we characterize the binding nature and the interacting moieties of ${\alpha}X$ I-domain and RAGE. Their binding requires divalent cations ($Mg^{2+}$ and $Mn^{2+}$) and shows an affinity on the sub-micro molar level: the dissociation constant of ${\alpha}X$ I-domains binding to RAGE being $0.49{\mu}M$. Furthermore, the ${\alpha}X$ I-domains recognize the V-domain, but not the C1 and C2-domains of RAGE. The acidic amino acid substitutions on the ligand binding site of ${\alpha}X$ I-domain significantly reduce the I-domain binding activity to soluble RAGE and the alanine substitutions of basic amino acids on the flat surface of the V-domain prevent the V-domain binding to ${\alpha}X$ I-domain. In conclusion, the main mechanism of ${\alpha}X$ I-domain binding to RAGE is a charge interaction, in which the acidic moieties of ${\alpha}X$ I-domains, including E244, and D249, recognize the basic residues on the RAGE V-domain encompassing K39, K43, K44, R104, and K107.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.23 no.5
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• pp.110-119
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• 2009

In this study magnetic fields near electric power lines with branch lines which have a arbitrary angle were derived and formulated by dipole antenna theory and could be calculated easily using the formula. It seems that those formula could be applicable to the consideration of magnetic fields during the design of distribution lines with branch lines. As an example those formulated equations on elements of magnetic fields were applied to a model of 3 phase distribution lines with branch lines and calculated by Matlab programs and the results were presented The analyzed results are follows. The resultant magnetic field is dominated by the componant By all over y-axis in the case of the smaller branched angle $\alpha$ and the lower observed point z. In case of ${\alpha}=\frac{\pi}{2}$[rad], the resultant field is affected by the componant Bx. The resultant field is dominated by the componant Bz at the vicinity of the power lines and it shows very large value at the branch line position of y-axis in case of ${\alpha}>\frac{\pi}{2}$.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.16 no.28
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• pp.137-143
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• 1993

Generally there are three methods to derive an approximation formula: 1) approching standard normal distribution by appropriate changing variable 2) using standardization variable for expansion 3) deriving approximation formula by direct method. This paper present correction terms having the form of $C_{1/v^{n/2}}/{\;}+{\;}C_2{\;}(n=1,2)$ with respect to $x^2_{\alpha}(v)$ distribution (${\nu}{\;}{\leq}{\;}30$), which minimize the error by EDA method and least square method.

• The Pure and Applied Mathematics
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• v.4 no.2
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• pp.105-110
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• 1997

The distribution of staff in a hierachial organization has been studied in a variety of forms and models. Results here show that the promotion process follows a binomial distribution with parameters n and $\alpha=e^{-pt}$ and the recruitment process follows a poisson distribution with parameter $\lambda$. Futhermore, the mean time to promotion in the grade was estimated.

• Ocean Science Journal
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• v.41 no.1
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• pp.11-29
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• 2006

We investigated species composition and spatial distribution of the euphausiid community in the Yellow Sea and identified the relationship with environmental factors (temperature, salinity, chlorophyll $\alpha$, nitrate, phosphate, and silicate) using bimonthly data from June, 1997 to April, 1998. The environment varied during the sampling period. In warm seasons, thermocline was well developed rendering lower temperature and higher salinity and nutrient concentrations in the bottom layer. During cold seasons the water column was well mixed and no such vertical stratification was noted. Horizontal distribution of temperature, however, differed slightly between near-coast and offshore areas because of the shallow depth of the Yellow Sea, and between southern and northern areas because of the intrusion of water masses such as Yellow Sea Warm Current and Changjiang River Diluted Water. Four euphausiid species were identified: Euphausia pacifica, E. sanzoi, Pseudeuphausia sp. and Stylocheron affine. E. sanzoi and S. affine were collected, just one juvenile each, from the southern area in June and December, respectively. Pseudeuphausia sp. were collected in the eastern area all the year round except June. E. pacifica occurred at the whole study area and were the predominant species, representing at least 97.6% of the euphausiid abundance. Further, the distribution pattern of the species was varied in regards to developmental stages (adult, furcilia, calyptopis, egg). From spring to fall, E. pacifica adults were abundant in the central area where the Yellow Sea Bottom Cold Water prevailed. Furcilia and calyptopis extended their distribution into nearly all the study area during the same period. From late fall to winter, adults were found at the near-coastal are a with similar pattern for furcilia and calyptopis. The distribution pattern of E. pacifica was consistent regarding temperature, salinity, and three nutrients during the sampling period, whereas chlorophyll $\alpha$ showed a different pattern according to the developmental stages. The nutrients should indirectly affect via chlorophyll $\alpha$ and phytoplankton concentration. With respect to these results, we presented a scenario about how the environmental factors along with the water current affect the distribution of E. pacifica in the Yellow Sea.

• Journal of Korean Society for Geospatial Information Science
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• v.9 no.1 s.17
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• pp.19-25
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• 2001

Satellite remote sensing, with its synoptic coverage, is used to evaluate the trophic state of large reservoir for agriculture in Kum River basin. The prediction model for chlorophyll-${\alpha}$ which was derived from Daecheong reservoir was applied to four LANDSAT TM imageries to generate a distribution map of trophic state. The chlorophyll-${\alpha}$ model was found to be reasonably reliable predictors for average trophic state value of reservoir. LANDSAT TM imagery data appears to have great utility in assessing trophic state of reservoir.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.9
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• pp.1245-1251
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• 2006

This study was carried out to determine the genetic relationships among seven west African goat breeds : Casamance Goat (Kolda, Senegal), Labe Goat (Fouta Djallon, Guinea), three Sahel Goat (Djoloff, Senegal ; Maradi, Niger; Gorgol, Mauritania) red Sokoto Goat (Maradi, Niger) and Guera goat (Atar, Mauritania).The polymorphism of six microsatellites and the ${\alpha}_{s1}$-casein locus was analysed. The six microsatellite loci were polymorphic with a mean number of alleles ranging from 2.71 to 4.0. At the ${\alpha}_{s1}$-casein locus, A and B were the most frequent alleles, which are known to be associated with a high level of protein synthesis. A neighbour-joining tree and a Principal Component Analysis were performed and the reliability of both methods was tested. Our study shows that the genetic relationships among the breeds analysed correspond to their geographical distribution and in addition, that the Labe Goat is strongly separated from the other breeds. Among the seven markers used, four have an effect on the distribution of breeds while three seem to be non-informative.

• 한국연소학회:학술대회논문집
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• 2002.06a
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• pp.163-170
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• 2002

The main objective of this study is to investigate the correlation of chemical structure and cetane number of reformed diesel fuels by ultrasonic irradiation. In order to analyze the effect of the chemical structure and the cetane number of reformed diesel fuels by ultrasonic irradiation, $^1H-NMR$ was used. From the study, following conclusive remarks can be made. 1) BI(=Branch Index), aromatics percentages, and $H_{\alpha}(={\alpha}-methyl$ functional group) of the reformed diesel fuels by ultrasonic irradiation decreased more than those of the conventional diesel fuel. 2) All the cetane numbers which were calculated from carbon type structure and hydrogen type distribution of the reformed diesel fuels increased more than those of the conventional diesel fuel. 3) Using predicated equation of cetane number caculated from carbon type structure is more reasonable than that caculated from hydrogen type distribution 4) BI, aromatics percentages, and $H_{\alpha}$ on both of conventional fuel and reformed diesel fuels by ultrasonic irradiation are inversely proportional to cetane number on these fuels.