• Food Science and Biotechnology
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• 제16권5호
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• pp.747-752
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• 2007

This study was carried out to identify and quantify the flavonoids from 6 different plant parts of Allium victorialis var. platyphyllum (AVP), including the flower, leaf, root, stem, flower stalk, and flower seed, using liquid chromatography/ mass spectrometry. Two major flavonoids were structurally identified as quercetin (3,5,7,3'4,'-pentahydroxyflavone) and kaempferol (3,5,7,4'-tetrahydroxyflavone) at contents of 11.8-25.8 and $6.0-64.4\;{\mu}g/mL$, respectively. In particular, the flower and root plant parts contained the highest amounts of quercetin and kaempferol compared to the other parts. We also assessed the recovery effects of each plant-part extract of AVP on gap junctional intercellular communication (GJIC) in WB-F344 cells by the scrape-loading and dye transfer (SL/DT) method. According to the results, GJIC was reduced by approximately 70.2% ($62.3{\pm}12.5$ cells) compared to the control ($209{\pm}9.5$ cells, 100%) when 12-O-tetradecanoylphorbol-13-acetate (TPA) was treated alone in the WB-F344 rat liver epithelial cells. However, the stem extract (0.2 mg/mL) restored GJIC to basal levels (92%, $204{\pm}2.3$ cells, p<0.01) and the flower extract (0.2 mg/mL) stimulated GJIC to 82.5% ($172.6{\pm}8.3$ cells, p<0.05), when applied together with the TPA.

• 한국임상수의학회지
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• 제27권3호
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• pp.225-231
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• 2010

고지방식이로 유발된 간과 신장의 손상에서 산마늘 메탄올 추출물의 효과를 알아보고자 하였다. 12주 동안 산마늘 메탄올 추출물 62.5, 125, 250 mg/kg/day를 투여한 결과 고지방식이로 유발된 혈청중의 AST, ALT, BUN, creatinine의 증가 억제와 간의 조직병리학적 변화를 감소시켜 지방간염과 신장의 손상이 현저하게 감소하였다. 또한, 항산화 효소인 superoxide dismutase, glutathione, catalase의 증가와 malondialdehyde의 감소로 항산화방어체계(antioxidant defense system)를 강화시켰다. 산마늘 메탄올 추출물 125 mg/kg 투여군은 대조군인 silymarin 100 mg/kg 투여군과 유사한 효과를 나타내었다. 결론적으로 산마늘 메탄올 추출물은 고지방식이로 유발되는 간과 신장의 손상을 완화하는 유용한 작용을 하는 것으로 나타났다.

• 한국임상수의학회지
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• 제28권3호
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• pp.280-286
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• 2011

본 실험은 고지방식이로 유발된 비만마우스에서 산마늘 잎 메탄올 추출물의 항비만 효과를 알아보고자 체중변화, 사료섭취량변화, 렙틴과 아디포넥틴 수치변화, 난소주위 지방무게 변화 측정과 지방세포의 조직병리학적 검사를 실시하였으며, 메트포민을 체중당 250 mg 투여한 약물대조군과 비교평가 하였다. 비만유발을 위하여 고지방식이를 13주간 공급하였으며, 고지방식이 공급 7일째에 증류수(비만대조군), 메트포민(약물대조군), 산마늘 잎 메탄올 추출물을 투여하였다. 비만대조군은 확연한 비만이 유발되었으며, 산마늘 잎 메탄올 추출물(62.5, 125, 250 mg/kg) 투여군에서는 이러한 비만이 유의적으로 감소하였다. 결론적으로 산마늘 잎 메탄올 추출물은 비만유발 마우스에서 비만개선작용을 나타내었으며, 나아가 인체에서도 유의한 비만개선작용을 할 것으로 생각된다.

• 한국산림과학회지
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• 제98권1호
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• pp.106-114
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• 2009

본 연구는 산마늘, 곰취, 곤달비를 대상으로 피음 수준을 전광 처리구(상대 투광율; 100%), 약피음 처리구(상대 투광율; 64~73%), 보통피음 처리구(상대 투광율; 35~42%), 강피음 처리구(상대 투광율; 9~16%)의 4단계로 달리하여 이들의 광합성 특성, 엽록소 형광반응 및 엽록소 함량을 조사 분석하였다. 세 식물의 광합성 능력은 7월에 가장 높았다. 산마늘은 광합성 능력과 광화학 효율이 보통피음 처리구(상대 투광율; 35~42%)에서 가장 높았다. 그러나 곰취와 곤달비는 전광 처리구(상태 투광율; 100%)에서 광합성 능력과 광화학 효율이 가장 높았으며, 피음 수준이 높아질수록 낮아지는 경향을 보였다. 총 엽록소 함량은 세 식물 모두 피음 수준이 높아질수록 유의적 차이를 보이며 높게 나타났다.

• 생약학회지
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• 제34권3호통권134호
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• pp.250-255
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• 2003

To develop Allium victorialis var. platyphyllum (Liliaceae) for an available functional food, pharmacological experiments on the extracts of this plant were undertaken in the rat treated with streptozotocin, poloxamer-407 and $CCl_4$. The two MeOH extracts were obtained from the leaves and the bulbs, respectively. The three agents, streptozotocin, poloxamer-407 and $CCl_4$, were treated to induce diabetes mellitus, hyperlipidemia and hepatic injury, respectively. Treatment with the leaf extract lowered blood glucose by 24.9% at 200 mg/kg (p.o.) in the STZ-treated rat and prohibited the increase of body weight, water consumption and food intake. This exσact also significantly decreased not only thε plasma cholesterol and triglyceride in the poloxamer-407-treated rat by 35.3% but also serum ALT by 49.0%. The potency was found by overall estimation on the experimental results as followings: 200 mg/kg leaf extract> 200 mg/kg bulb extract> 100 mg/kg leaf exσact> 100 mg/kg bulb extract. These results suggested that this plant might have the availability for a functional food. It was also suggested that the leaves are more effective than the bulbs for the functional vegetable, especially in hyperlipidemia and hepatic injury.

• Journal of Plant Biotechnology
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• 제49권3호
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• pp.213-221
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• 2022

Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.

• 한국자원식물학회지
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• 제24권2호
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• pp.150-159
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• 2011

This study was conducted to gather the basic data on the alpine leek (Allium victorialis) for the expand of consumption and the production of its manufactured goods. Amino acid content in alpine leek leaves and various physiological activities were examined. Seventeen component amino acids and 38 free amino acids from alpine leek leaves were analyzed, and the total contents were 2,693.28 mg/100g for component amino acids and 535.39 mg/100g for free amino acids. Total phenolic compounds in the leaves of alpine leek showed the highest level from the methanol extract (37.7 mg/l), and followed by ethanol extract (31.9 mg/l) and hot water extract (25.4 mg/l). Total flavonoid contents in 1,000 mg/l extract was the highest in the methanol extract (22.2 mg/l). DPPH radical scavenging activity at 1,000 mg/l extract was high in the order of ethanol extract (51.6%), methanol extract (47.3%) and hot water extract (37.2%). nitrite radical scavenging activity Methanol extract from Allium victorialis leaves was the highest nitrite radical scavenging activity (79.5%). Hyperplasia suppression of lung cancer cells (Calu-6) and gastric cancer cells (SNU-601) by the methanol extract from the bulb of alpine leek were 99.9% in the extracting concentration of over 200 mg/l. No significant difference in antimicrobial activity among the 3 different solvents and extract concentrations was observed, and the inhibition zones against the gram-positive and negative microorganisms were ranged from 8.23 to 10.15 mm. It was concluded that physiological activities in a human body could be improved by the intake of alpine leek as a pharmaceutical material, and that it would be useful for the prevention of health risk such as lung and gastric cancers.

• 농업생명과학연구
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• 제46권3호
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• pp.19-26
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• 2012

본 연구는 산마늘의 임간재배를 위한 기초 환경자료를 제공하고자 실시하였다. 울릉도 산마늘은 너도밤나무, 우산고로쇠나무, 섬잣나무, 솔송나무, 소나무, 곰솔, 섬피나무, 두메오리나무 군락에 자생하고 있으며, 입지환경은 평균 해발고도 563.4 m, 평균 경사는 $27.8^{\circ}$ 이었다. 토성은 사토, 사질양토, 양질사토, 양토가 다양하게 나타났으며, 유기물층의 깊이는 평균 6 cm, 토양 pH는 5.40, 유효인산은 18.5 ppm, 양이온 치환용량은 $18.6cmol_c/kg$으로 나타났다. CCA분석에 의한 입지와 토양인자와의 상관관계를 보면 너도밤나무군락은 탄소, 질소, 모래함량, 양이온치환용량과 정의 상관관계를 나타내었다.

• 한국산림과학회지
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• 제100권4호
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• pp.565-576
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• 2011

본 연구는 울릉도와 함백산의 산마늘 개체군 자생지를 대상으로 48개의 조사구($10m{\times}10m$)를 설치하여 임분구조를 밝히고자 식생유형을 분류한 다음, 각 유형별 중요치, 종다양도, 종간연관관계, 군집유사도를 분석하여 임분구조 특성을 밝혔다. ZM 식물사회학적 방법으로 종조성에 의해 식생유형을 분석한 결과, 1개군락군 2개군락 2개군 3개소군으로 총 5개의 식생단위로 나누어졌으며, 층위별 중요치를 분석한 결과, 너도밤나무군(식생단위 1, 2, 3)에서 산마늘의 중요치가 비교적 높았으며, 낙엽활엽수가 우점하는 단위에서 산마늘의 중요치가 높게 나타났다. 종간연관관계를 분석한 결과, 산마늘과 양의 상관관계를 가지는 종은 계곡성 식물종과 아고산대 식물종이었고, 산마늘이 생육하는데 있어 토습과 공중습도가 중요한 환경요인으로 판단되었다.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2004년도 춘계학술대회
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• pp.237-237
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• 2004