• 생명과학회지
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• 제23권9호
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• pp.1109-1117
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• 2013

본 연구에서는 인체 폐암 세포인 A549를 사용하여 택란 메탄올 추출물의 항암활성과 그 분자적 기전에 관하여 연구하였다. 먼저 택란 추출물이 A549의 세포증식에 미치는 영향을 알아본 결과 처리 농도 및 시간 의존적으로 A549의 성장이 저해되었으며, 세포 주기 변화를 분석한 결과 강력한 G1 arrest가 유도되는 것을 확인하였다. 이러한 택란 추출물에 의한 G1 arrest는 세포주기 조절 단백질인 Cyclin D1, Cyclin E 및 Cyclin-dependent kinase인 CDK2, CDK4, CDK6의 발현 감소와 연관되어 있었다. 또한 택란 추출물에 의한 CDK/Cyclin complex의 발현 저해는 DNA 손상에 의해 활성화되는 CHK2의 활성화 형태인 p-CHK2의 발현 증가에 따른 CDK 활성화 효소인 Cdc25A phosphatase의 발현 억제에 의해 나타나는 결과로 사료된다. 반면 종양억제유전자인 p53 및 CDK 억제제인 p21과 p27의 발현량은 증가되지 않았다. 이러한 결과들로부터 택란 추출물은 DNA damage에 의한 ATM/CHK2/Cdc25A/CDK2 pathway를 통해 A549의 G1 arrest를 유도하여 세포의 증식을 억제할 것으로 판단되며, 이때 택란 추출물에 의해 유도되는 G1 arrest는 p53 비의존적인 경로일 것으로 사료된다. 본 연구결과는 택란이 Cdc25A를 target으로 하는 새로운 항암활성 소재로서 사용될 수 있는 가능성을 시사한다. 또한 본 연구결과는 택란 추출물의 세포주기 조절에 의한 항암기전을 이해하고 향후 지속적 연구를 하는 데 있어서 귀중한 기초자료로 사용될 수 있을 것이다.

• 사상체질의학회지
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• 제12권1호
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• pp.240-259
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• 2000

1. 연구배경 및 목적 소음인(少陰人) 신정방의 하나인 독삼관계이중탕(獨參官桂理中湯)의 효능(效能)와 활용(活用)에 대한 임상적 연구를 통하여 사상체질병증(四象體質病證)의 이해(理解)와 방제(方劑)의 운용(運用)에 도움을 주고자 한다. 2. 방법 1997년 8월부터 2000년 6월까지 본원에 내원(來院)한 환자들중 독삼관계이중탕(獨參官桂理中湯) 5첩이상 투여되고 경과(經過) 관찰(觀察)이 가능했던 인후통(咽喉痛)를 주소증(主訴症)으로하는 16명(남자 1명, 여자 15명)을 대상으로 하여 임상적(臨床的) 유의성(有意性)를 검증(檢證)하였다. 16명은 체간측정법(體幹測定法)에 의한 체질감별상(體質鑑別上) 소음인(少陰人)로 판정한 15명, 태음인(太陰人) 1명이었다. 3. 결과 독삼관계이중탕(獨參官桂理中湯)를 소음인(少陰人) 20대에서 60대에 걸쳐 사용하였으며, 대상환자(對象患者)들의 주소증(主訴症)는 인후통(咽喉痛), 편도선염(扁桃腺炎), 비염(鼻炎), 구조(口燥), 구고(口苦), 건해(乾咳), 구내염(口內炎), 대장파한증, 경년기증후군(更年期證候群) 등 28종류이었다. 치료효과(治療效果)의 분포는 쾌유(快癒) 56.25%, 양호(良好) 18.75%, 호전(好戰) 6.25%, 별무효과(別無效果) 12.50%, 악화(惡化) 6.25%(태음인의 경우)이었다. 전신증상의 특징으로 설태(舌苔)가 황(黃), 백(白), 황흑(黃黑)하게 나타나거나 수면장애(睡眠障碍), 한출이상(汗出異常)이 있는 경우도 있었다. 처방(處方)을 활용(活用)할 때는 처방(處方)의 변경없이 독삼관계이중탕(獨參官桂理中湯)만을 사용하였으며 처방내용 중 인삼(人蔘)의 중량(重量)을 20그람 또는 32그람으로 하여 분여(扮與)하였다. 독삼관계이중탕(獨參官桂理中湯)의 치료효과(治療效果)는 인삼(人蔘)의 중량(重量)에 관계없이 유효(有效)하였으며 효과발현시간(效果發顯時間)은 연령(年齡)에 관계없이 복용량(服用量)의 변화(變化)에 따라 신속(迅速)하였다. 부작용(副作用)로는 혈압상승(血壓上升), 두통(頭痛), 결흉(結胸)등이 있었다. 4. 결론 독삼관계이중탕(獨參官桂理中湯)는 인후통(咽喉痛)에 특효(特效)가 있었다.

• 생명과학회지
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• 제29권11호
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• pp.1208-1217
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• 2019

방풍의 화합물은 동양 전통 의학에서 사용되고 있는 식물로 항염증, 비염, 미백, 아토피, 항알레르기 및 각종피부질환에 사용되고 있다. 본 연구는 한국 전통발효식품에서 분리된 Lactobacillus plantarum BHN-LAB 33를 이용해 발효한 방풍 추출물의 항산화 효과를 조사하였다. L. plantarum BHN-LAB 33 발효는 $37^{\circ}C$에서 3일간 발효하였으며, 발효하지 않은 방풍 추출물에 비해 L. plantarum BHN-LAB 33 발효 방풍 추출물에서 총 폴리페놀 함량은 약 14%, 총 플라보노이드 함량은 약 09% 증가된 것을 확인하였다. 또한, SOD 유사활성능, DPPH radical 소거활성능과 ABTS radical 소거활성능, 환원력은 각각 약 70%, 80%, 45% 및 39% 항산화능이 증가하는 것을 확인하였다. 또한, tyrosinase 저해 활성은 44% 증가되는 것을 확인하였다. 이 결과를 통해 L. plantarum BHN-LAB 33을 이용한 생물전환을 통한 방풍 발효 추출물의 항산화능 증대에 효과적인 것을 확인하였다. 또한, 생물전환기술은 생리활성을 증대시키기 위한 매우 유용한 기술임을 확인하였으며, 생물전환기법을 통한 기능성 식품소재 및 화장품 소재 개발 및 응용이 가능할 것으로 기대된다.

• 생명과학회지
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• 제20권4호
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• pp.511-518
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• 2010

전 세계적으로 알레르기 환자가 증가하고 있고, 특히 운동이 알레르기 질환을 증가 시켜 운동 유발성 알레르기에 대한 연구가 주목되고 있다. 운동 유발성 알레르기 질환은 운동 중과 운동 후 혈관부종, 두드레기, 호흡, 위와 장계의 증후 및 아나플락시스 등이 나타나는 질환이다. 따라서 본 연구는 운동 유발성 알레르기 질환의 분석 및 비타민 C와 catalase 투여에 대한 항 알레르기 효과와 활성 산소종 생성 조절제 들의 상호작용을 규명하고 전반적인 운동 유발성 알레르기 질환의 치료효과를 규명하는데 그 목적이 있다. 연구방법으로는 무병원성환경에서 사육한 생후 7주령의 BALE/c마우스(Female)를 그룹핑 하여 통제군과 훈련군에게 각각 알레르기를 유발한 후, 서로 다른 기간의 운동 효과를 분석하였고, 운동 유발성 알레르기 군에게 비타민c와 catalase를 투여하여 투여 효과를 분석하였다. 본 연구결과 운동 유발성 알레르기 질환은 훈련기간이 늘어남에 따라 증가함을 알 수 있었고, 이러한 운동 유발성 알레르기 질환과 쇼크사는 복강 림프구에서 발현하는 ROS는 큰 연관이 있다는 것, 그리고 운동 유발성 알레르기 치료 효과는 catalase를 투여하는 것 보다 비타민을 투여하는 것이 더욱 효과적임을 알 수 있었다. 추후 이에 대한 원인을 규명하는 기전적 연구가 필요할 것으로 여겨진다.

• 한방안이비인후피부과학회지
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• 제25권1호
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• pp.55-74
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• 2012

Objective : The purpose of this study is to investigate the skin disease in Sanghanron"傷寒論" and Geumgweyoryak"金匱要略". Methods : We conducted a study on the original text paragraphs of Sanghanron and Geumgweyoryak containing the skin disease and analysis of Woo, Oh, Sung, Zhang, etc. We drew a parallel between skin disease from Sanghanron and Geumgweyoryak and matching diagnoses from western medicine. Results : The results were as follows. 1. In Sanghanron and Geumgweyoryak pruritus was related to psychogenic pruritus and similar to skin disease caused by decrease of sweating, dry skin in cholinergic urticaria, atopic dermatitis and eczema, etc. in western medicine. 2. In Geumgweyoryak ichthyosis was caused by woman's disease, after menopause, chronic disease and malnutrition and related to winter itch and xerotic eczema in western medicine. 3. In Sanghanron Goose bumps, keratosis follicularis, acute and chronic urticaria and cold urticaria were occurred because of poorly managed fever care and side effect of antibiotic. 4. In Geumgweyoryak red face was similar to face flushing and side effects, which is after drug and laser treatment on face, in western medicine. 5. In Geumgweyoryak urticaria was, in a broad sense, skin disease with pruritus and secondary infection resulted from scratch. Skin diseases with pruritus are similar to urticaria, dermatitis herpetiformis, lichen planus, atopic dermatitis, contact dermatitis, psoriasis, nummular eczema, lichen simplex chronicus, prurigo nodularis, neurodermatitis and internal disease with pruritus in western medicine. 6. In Geumgweyoryak spots in the skin were caused by fever and similar to allergic purpura and SLE symptom in western medicine. 7. In Geumgweyoryak bullous disease with yellowish discharge was similar to eczema, pustule, atopic eczema, etc. in western medicine. 8. In Geumgweyoryak repeated inflammation of oral cavity, genitals, eyes and skin was similar to Behcet's disease in western medicine. 9. In Sanghanron and Geumgweyoryak boil was similar to abscess and acute lymphadenitis in western medicine caused by oily foods and infections. 10. In Geumgweyoryak swelling and boil were occurred in a poor health. Damage from metalic material could cause a convulsion and was similar to tetanus in western medicine. Conclusion : We analyzed the original text paragraphs of Sanghanron and Geumgweyoryak and explanations about skin disease. As a results, we found out etiology, pathogenesis, treatments of the skin disease in Sanghanron and Geumgweyoryak. Further we compared with western medicine to develop better understanding of the skin disease.

• 농업과학연구
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• 제45권3호
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• pp.437-446
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• 2018

Lactoferrin is an iron-binding glycoprotein which is present in colostrum, milk, and other body secretions. Lactoferrin activities are associated with inflammatory and immune responses. The aim of this study was to investigate the effect of lactoferrin hydrolysates (LH) on the production of immunomodulatory factors such as inflammatory related cytokines (tumor necrosis factor $(TNF)-{\alpha}$, interleukin $(IL)-1{\beta}$, interleukin (IL)-6, and interleukin (IL)-13) in Raw264.7 cells, which originated from murine macrophages. The results show that the Raw264.7 cells cultured in 3 types (whole, and above and below 10 kDa) of lactoferrin hydrolysates (LH) did not show any cytotoxicity in the cells. $TNF-{\alpha}$ decreased dose-dependently to 1,500 - 2,000 ng/mL by treatment with the 3 types of LH at 1, 50, $100{\mu}g/mL$, whereas the positive control, lipopolysaccharide (LPS), and negative control produced 2,450 and 1,000 ng/mL of $TNF-{\alpha}$, respectively, in the Raw264.7 cells. The treatment with the 3 types of LH (whole and above and below 10 kDa) at $50{\mu}g/mL$ produced about 20 - 28 ng/mL of $IL-1{\beta}$ at 3, 6, and 9 h, respectively, while the negative control produced 7 ng/mL, and LPS as the positive control produced 48 - 60 ng/mL. $TNF-{\alpha}$ and IL-6 expression was decreased dose-dependently by the 3 types of LH. The mRNA levels of IL-13 were slightly increased dose-dependently by the whole and above 10 kDa LH, but decreased dose-dependently by the below 10 kDa LH in the Raw264.7 cells. The results show that LH had immunomodulating effects on cytokine production in anti- and pro-inflammatory reactions as well as anti-allergic reactions.

• 대한한방소아과학회지
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• 제32권3호
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• pp.72-89
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• 2018

Objectives The objective of this study is to investigate the application of Sifeng point (EX-UE 10) in children and to make suggestions for domestic research and clinical application by reviewing relevant randomized controlled trials. Methods Eight electronic databases including English, Chinese, Korean databases were comprehensively searched for randomized controlled trials evaluating the effects of Sifeng point in children up to May 2, 2018. We extracted the year of publication, country, target diseases or symptoms, location of Sifeng, acupuncture method; such as acupuncture tool and depth of insertion, and frequency, number, and duration of the treatment. Results Fifty studies were included for analysis. All the included studies were conducted in China, and studies were published from 1962 to 2017. The most frequent target symptom was anorexia (46%). In addition, Sifeng point was used for digestive diseases or symptoms such as diarrhea, constipation, and mesenteric lymphadenitis, as well as respiratory diseases or symptoms such as cough, cold, acute bronchiolitis, recurrent respiratory tract infections, bronchopneumonia, and asthmatic bronchitis. Also, allergic diseases such as chronic urticaria, and other variety of symptoms such as malnutrition, fever, and iron-deficiency anemia were treated with the Sifeng point. The location of Sifeng points was inconsistent, and the most common location of the Sifeng point was the midpoint of transverse creases of the proximal interphalangeal joints of the index, middle, ring and little fingers (84.2%). The most commonly used acupuncture tool was three-edged needle (40.9%). All included studies have the method of squeezing out blood or mucus from the Sifeng points. The treatment was mostly conducted once per week (35.4%), mostly repeated for 4 times (32.6%). The most common treatment duration was 4 weeks (18.6%). Conclusions We could identify acupuncture method and various indications for the Sifeng points treatment. Based on this study, there is a need for the clinical application and related researches on the Sifeng points in children in Korea. In addition, the treatment location of Sifeng point should be standardized.

• BMB Reports
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• 제48권3호
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• pp.178-183
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• 2015

Dendritic cells play an important role in determining whether na${\ddot{i}}$ve T cells mature into either Th1 or Th2 cells. We determined whether heat-shock protein X (HspX) purified from Mycobacterium tuberculosis regulates the Th1/Th2 immune response in an ovalbumin (OVA)-induced murine model of asthma. HspX increased interferon-gamma, IL-17A, -12 and transforming growth factor (TGF)-${\beta}$ production and T-bet gene expression but reduced IL-13 production and GATA-3 gene expression. HspX also inhibited asthmatic reactions as demonstrated by an increase in the number of eosinophils in bronchoalveolar lavage fluid, inflammatory cell infiltration in lung tissues, airway luminal narrowing, and airway hyper-responsiveness. Furthermore, HspX enhanced OVA-induced decrease of regulatory T cells in the mediastinal lymph nodes. This study provides evidence that HspX plays critical roles in the amelioration of asthmatic inflammation in mice. These findings provide new insights into the immunotherapeutic role of HspX with respect to its effects on a murine model of asthma.

• 동의생리병리학회지
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• 제21권5호
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• pp.1210-1218
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• 2007

Recently Atopic Dermatitis(AD) is increasing along with allergic disease. At present, there is no infallible cure for AD. Then AD patients undergo great suffering. This study is carried out to see whether or not the administering Danggwieumja(DG) along with Samhwangseje-gamibang(SG} as a medicine for external aplication, is effective in treating atopic dermatitis. To examine the effectiveness of the above prescription, the author made an observation of diverse immune responses. through the model of NC/Nga atopic mice. Results provided evidence that the DG administration along with SG can be used as a treatment means to atopic dermatitis. The results are as follows: The extent of Clinical skin severities in 13 and 16 week old NC/Nga mice treated with DG and SG, were reduced by 50.9%, 53.9% respectively, compared to the control NC/Nga mice with no drug treatment. IgE, IL-4, IL-5, IL-6, IgM and IgG1 levels in the serum of the NC/Nga mice treated with DG and SG were significantly decreased compared to those of the untreated control mice. In contrary, to the $IFN-{\gamma}$ level, significantly increased. The spleen weight of the NC/Nga mice treated with DG and SG significantly decreased compared to those of the untreated control mice. CCR3 gene expression in the skin tissue of NC/Nga mice treated with DG and SG were highly decreased, and the IL-6 expression significantly decreased, and the $IFN-{\gamma}$ gene expression increased compared to those of the untreated control mice. Histological observation of the ear and dorsal skin tissue of the NC/Nga mice treated with DG and SG, showed that the extents of inflammation and infiltrated immune cells in the epidermal tissue and dermis, were highly reduced compared to those of the untreated control mice. In the model inducing COX-2 activity in RAW 264.7 cell, the denser DG became, the more COX-2 activity was inhibited, compared to those of the untreated control group. $IL-1{\beta}$, and $TNF-{\alpha}$, IL-6 gene expression in RAW 264.7 cell with DG, significantly decreased, compared to those of the untreated control group. According to the assessment of cell toxicity in L929 cell, the rate of cell multiplication increased by 3% in consistency to 100ppm of DG compared to the untreated control group and in more than the 200 ppm consistency, cell toxicity was occurred.

• 대한한의학회지
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• 제24권1호
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• pp.169-180
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• 2003

Background : Allergic asthma is thought to be mediated by $CD4^{+}$ T lymphocytes producing the Th2-associated cytokines. According to investigations of lung biopsies and respiratory secretions from patients, $CD4^{+}$ T cells and eosinophils are the main features of the inflammatory process. Object : This study aimed to find an inhibition effect on allergens induced by JCT (Jungchun-tang) and JCTG (Jungchuntanggagambang) through the change of $CD4^{+}$ T cells and $CD8^{+}$ T cells in BALF of rat, and to see the change of IgE m serum. Materials and Methods : Laboratory rats were primary sensitized with OA (ovalbumin); on day 1, rats of a control group and a sample group (SBP group) were systemically immunized by subcutaneous injection of 1mg OA and 300mg of A1(OH)3 in a total volume of 2 ml saline. The rats of the sample group were orally administered with an SBP water extract for 14 days after primary immunization. On day 14 after the systemic immunization, rats received local immunization by inhaling 0.9% saline aerosol containing 2%(wt/vol) OA. A day after local immunization, BAL fluid and serum were collected from the rats. Total cells, lymphocytes, $CD4^{+}$ T cells, $CD8^{+}$ T cells, and $CD4^{+}$/$CD8^{+}$ ratio in the BALF, and IgE level in serum were measured and evaluated. Results : L Total cell in BALF of rat : JCT was observed to be significantly reduced but JCTG had no significant difference in comparison with the control group. 2. Lymphocytes in BALF of rat : JCT and JCTG were observed to be significantly reduced in comparison with the control group. 3. $CD4^{+}$T cells in BALF of rat : JCT was observed to be more significantly reducing than JCTG in comparison with the control group. 4. $CD8^{+}$T cells in BALF of rat : JCT and JCTG were observed not to be significantly different than in the control group. 5. $CD4^{+}/CD8^{+}$ ratio in BALF of rat : JCT and JCTG were observed not to be significantly different than in the control group. 6. The IgE level in serum : JCT and JCTG were observed to be significantly reduced in comparison with the control group. Conclusion : This study shows that JCT inhibits allergen-induced specially select $CD4^{+}$T cell channel in BALF of rat.