BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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Heat shock protein X purified from Mycobacterium tuberculosis enhances the efficacy of dendritic cells-based immunotherapy for the treatment of allergic asthma

  • Kim, Hye-Young (Department of Pediatrics, Pusan National University School of Medicine, Medical Research Institute of Pusan National University Hospital) ;
  • Kang, Hyun Kyu (Department of Microbiology and Immunology, School of Medicine, Pusan National University) ;
  • Cho, Joon (Department of Neurosurgery, Konkuk University Hospital) ;
  • Jung, In Duk (Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, KU open innovation center and School of Medicine, Konkuk University) ;
  • Yoon, Gun Young (Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, KU open innovation center and School of Medicine, Konkuk University) ;
  • Lee, Min-Goo (Department of Physiology, College of Medicine, Korea University) ;
  • Shin, Sung Jae (Department of Microbiology, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine) ;
  • Park, Won Sun (Department of Physiology, School of Medicine, Kangwon National University) ;
  • Park, Jong-Hwan (Department of Biochemistry, College of Medicine, Konyang University) ;
  • Ryu, Seung-Wook (Cell Signaling and Bioimaging Laboratory, Department of Bio and Brain Engineering, KAIST) ;
  • Park, Yeong-Min (Department of Immunology, Lab of Dendritic Cell Differentiation & Regulation, KU open innovation center and School of Medicine, Konkuk University) ;
  • You, Ji Chang (National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea)
  • 투고 : 2014.12.04
  • 심사 : 2014.12.26
  • 발행 : 2015.03.31
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초록

Dendritic cells play an important role in determining whether na${\ddot{i}}$ve T cells mature into either Th1 or Th2 cells. We determined whether heat-shock protein X (HspX) purified from Mycobacterium tuberculosis regulates the Th1/Th2 immune response in an ovalbumin (OVA)-induced murine model of asthma. HspX increased interferon-gamma, IL-17A, -12 and transforming growth factor (TGF)-${\beta}$ production and T-bet gene expression but reduced IL-13 production and GATA-3 gene expression. HspX also inhibited asthmatic reactions as demonstrated by an increase in the number of eosinophils in bronchoalveolar lavage fluid, inflammatory cell infiltration in lung tissues, airway luminal narrowing, and airway hyper-responsiveness. Furthermore, HspX enhanced OVA-induced decrease of regulatory T cells in the mediastinal lymph nodes. This study provides evidence that HspX plays critical roles in the amelioration of asthmatic inflammation in mice. These findings provide new insights into the immunotherapeutic role of HspX with respect to its effects on a murine model of asthma.

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피인용 문헌

  1. Microbiome and Asthma: What Have Experimental Models Already Taught Us? vol.2015, 2015, https://doi.org/10.1155/2015/614758
  2. Effect of vitamin D3 on maturation and antigen-presenting function of dendritic cells treated with Mycobacterium tuberculosis vol.9, pp.1, 2016, https://doi.org/10.1016/j.apjtm.2015.12.011