• 제목/요약/키워드: alkaline method

검색결과 672건 처리시간 0.03초

호르텐스극구흡충에서 Alkaline Phosphatase 및 Acid Phosphatase의 특성 (Characteristics of Alkaline and Acid Phosphatase in Echinostoma hortense)

  • 양용석;김인식;임지애;강성구;박주연
    • 대한의생명과학회지
    • /
    • 제5권1호
    • /
    • pp.119-129
    • /
    • 1999
  • 호르텐스극구흡충 성충 (adult worm)을 재료로 하여 acid phosphatase (Acp)와 alkaline phosphatase (Alp)의 조직 세포에 대한 분포 및 동위효소의 특성을 규명하기 위하여 Gomori lead nitrate 및 Gomori calcium방법 그리고 전기영동법 등을 시행하였다. 조직 내의 Acp및 Alp의 분포는 충체의 소장 (intestine)과 난황선 (vitellaria)그리고 인두 (pharynx)에서 강한 반응을 나타냈다. 동위효소 유형은 Acp에서 179.5 및 209.4 kDa 두 개의 분획이 분리되었고, Alp는 145.9, 207.5 및 220.8 kDa의 세 개의 분획이 분리되었다. 열에 대한 안정도는 Alp가 9$0^{\circ}C$에서 12초경과 후에 완전히 불활성화 되었으며, Alp의 최적 온도 및 pH는 4$0^{\circ}C$와 pH 9이었고 Acp의 치적 pH는 5였다. 그리고 Acp및 Alp의 최대 활성도 (unit)는 Acp가 71 범위였고, Alp는 189의 범위에 있었다. 이상에서와 같이 호르텐스극구흡충은 phosphatase가 소화관과 난황선에서 주로 분포하고 있었으며 숙주 체내에서 소화관을 중심으로 대사가 활발하게 이루어지고 있음을 알 수 있었다.

  • PDF

Kluyverromyces fragilis의 Alkaline Phosphatase 유전자의 구조 분석 (Structural Analysis of Alkaline Phosphatase Gen from Kluyveromyces Fragilis)

  • 박수영;황선갑;하상철;김종국;박완;홍순덕
    • 한국미생물·생명공학회지
    • /
    • 제22권1호
    • /
    • pp.31-36
    • /
    • 1994
  • From the pSKH201 plasmid which had been previously cloned in our laboratory, a 3.0kbp insert DNA encoding the alkaline phosphatase of Kluyveromyces fragilis was cleaved with several restriction endonucleases and ligated int the appropriate sites of M13mp18/19 vectors and sequenced by Sanger's dideoxy chain termination method. The sequence contained a 1,638 bp open reading frame(ORP) whose similarities in nucleotide, when compared with those of Saccharomyces cerevisiae and Escherichia coli by GENETYX program, were found to be 61% and 46%, respectively. The deduced amino acid sequence consists of 546 amino acids and contains several homologous regions in the alkaline phosphatases of E. coli, S.cerevisiae and human placenta.

  • PDF

PET직물의 NaOH/4급암모늄염 혼합액에 의한 물성변화 (The Change of the Physical Properties of PET Fabrics Treated in NaOH/Quarternary Ammonium Salt Compound Solution)

  • 오수민;조승식
    • 한국의류학회지
    • /
    • 제18권3호
    • /
    • pp.287-297
    • /
    • 1994
  • The purpose of this study is to investigate the Improvement of fiber surface, tenacity & elogation, fabric counts, thickness, handle, moisture regain, static voltage, handle, dyeability when polyester fabrics are treated with NaOH solution adding quartemary ammonium salt (Benzyl dimetyl dodecyl ammonium chloride . BDMDAC) The results are as follows. I. In regard to the method of processing VET, when the PET fabrics are alkaline-hydrolyzed adding BDMDAC, the weight loss of PET fabrics increased remarkably to the increse of BDMDAC concentration than when they are treated only in NaOH solution. When PET fabrics are alkaline-hydrolyzed the amount of BDMDAC as the catalyst is proper lg/1 II. The change in physical & chemical properties of alkaline-hydrolyzed PET fabrirs. 1. As the amount of the weight loss on PET fabrics increased, the void space of the PET yale increased but tenacity & elongation and thickness decreased. 2. The fabric counts of PET increased due to shrinkage by alkaline-hydrolydzing. 3. As the amount of of the weight loss on PET fabrics increased. NUMERI, FUKURAMI, increaseed and KOSHI decreased and Total hand value(T.H.V) in all cases increaseed. When the weight Ioss is 30.9% T.H.V. is best. 4. Moisture regain of PET fabrics a little increased by alkaline-hydrolyzing treatment. As the weight loss increased, static voltage is decreased. 5. The last dye absorption is different according to the degree of the Affinity. In regard to the difference of dye color, the dyestuff of low molecular weight dyed for deep color.

  • PDF

연소법에 의한 rare earth doped doped alkaline earth aluminates 형광체의 발광특성 (Luminescence behaviour of rare earth doped alkaline earth aluminates synthesized by combustion method)

  • 정영호;박진원;박조용;;;명광식;한상도
    • 한국전기전자재료학회:학술대회논문집
    • /
    • 한국전기전자재료학회 2002년도 추계학술대회 논문집 Vol.15
    • /
    • pp.581-584
    • /
    • 2002
  • A new method for the preparation of lanthanide ions activated strontium aluminates phosphor by combustion method has been proposed. Combustion method consist of the redox reactions between the respective metal nitrates and urea in a preheated funace at $500^{\circ}C$. The luminescence behavior of the phosphor was studied and compared with corresponding phosphor prepared by conventional method. Effect of $Mg^{2+}$ ion concentration in strontium aluminate phosphor was investigated and the maximum luminescence of about $100cd/m^2$ was obtained. This method gave better brightness and small size to the phosphor than made by conventional method.

  • PDF

소나무 수피의 총합적 이용 (제5보) -소나무 수피의 알칼리성 아황산염-안트라퀴논에 의한 탈리그닌- (Utilization of Pine Bark (Part 5) -Alkaline Sulfite-Anthraquinone Delignification of Pine Bark-)

  • 문성필;박성천
    • Journal of the Korean Wood Science and Technology
    • /
    • 제27권2호
    • /
    • pp.46-52
    • /
    • 1999
  • 소나무 수피를 알칼리성 아황산염-안트라퀴논(AQ) 증해하고, 고도로 탐리그닌 시킬 수 있는 조건을 검토하였다. 수피의 탈리그닌은 약알칼리성 아황산염 증해 조건에서보다 알칼성 아황산염 증해 조건에서 용이하였다. 그러나 알칼리성 아황산염 증해조건에서도 수피리그닌의 90%이상을 용출시키기는 어려웠다. AQ는 알칼리성 아황산염 증해 조건에서 수피의 증해 및 탈리그닌 속도를 현저하게 향상 시킬 뿐만 아니라 탈리그닌 선택성도 개선시켰다. 결과적으로 수피에 대하여 0.2% AQ의 첨가로 90%이상의 탈리그닌이 가능하였다. 수피 알칼리성 아황산염-AQ증해시 증해온도 및 NaOH의 투여량은 탈리그닌 선택성에 대해서 큰 영향을 미치지 못하였다. 그러나 $Na_2SO_3$ 투여량은 수피의 탈리그닌 선택성에 큰 영향을 미쳐 $Na_2O$로서 30%의 $Na_2SO_3$ 투여조건에서 가장 양호한 탈리그닌을 나타내었다.

  • PDF

알칼리성 모발처리제를 이용한 후처리제 개발 (Development of an After-treatment Agent, Using an Alkaline Hair Treatment)

  • 김혜균
    • 디지털융복합연구
    • /
    • 제15권1호
    • /
    • pp.509-517
    • /
    • 2017
  • 최근, "웰빙" 바람을 타고, 주로 치료목적으로만 이용 되어온 이온수의 효능이 알려지면서, 이에 대한 관심이 조금씩 높아지고 있다. 이온수는 물을 전기분해 하면 쉽게 얻을 수 있는데, 전해질의 첨가 유 무와 이온 선택성 격막의 유 무에 따라 강산성, 강알칼리성, 중성 또는 알칼리성, 약산성, 약알칼리성 등의 이온수가 생성된다. 알칼리성 전해 이온수는 체내의 때를 씻게 해 주어 성인병의 증세를 서서히 없애주고, 신진대사와 혈액 점도를 낮추는데 중요한 기능을 한다. 현재 펌 촉진제의 알칼리성분을 사용하는 분야는 전무한 상태이며, 뷰티 시장의 향상과 시간단축으로 인한 수익 창출이 가능하며, 고객에게는 모발의 손상이 없고 모발의 잡균들을 제거함으로써 고객의 거친 모발에 에너지와 영양을 충전하여 손상된 모발을 빠르게 복구 할 수 있으며 염색/펌 시술 후 손상도니 머릿결을 복구 와 화학성분 제거 및 끈적임 없이 매끄럽고 윤기 있는 머릿결을 유지 할 수 있는 고효율의 알칼리성 모발후 처리제의 개발이 절실하다.

냉이온수기 냉각시스템에 관한 열유동 해석 (Thermal-flow Analysis of the Cooling System in the Medicated Water Electrolysis Apparatus)

  • 전성오;이상준;이종철;김윤제
    • 한국유체기계학회 논문집
    • /
    • 제14권3호
    • /
    • pp.33-38
    • /
    • 2011
  • Medicated water electrolysis apparatus, which electrolyzes water into acidic water and alkaline water, was in the spotlight as becoming known the effect of alkaline water. It is known as good for health as removing active oxygen in the human's body and promoting digestion. But, the customers could not get that desired water temperature because these apparatuses are directly connected with a water pipe. So, the cooling system was developed for controlling the temperature of the alkaline water. One of the typical way is to store water in water tank and control the temperature. But, in this way, storing water can be polluted impurities coming from outside. For protecting this pollution, the cooling system based on indirect heat exchange method through phase change between water and ice was developed. In this study, we have calculated efficiency of the cooling system with phase change by experiment and commercial CFD(Computational Fluid Dynamics) code, ANSYS CFX. To consider the effect of latent heat that is generated by melting ice, we have simulated two phase numerical analyses used enthalpy method and found the temperature, velocity, and ice mass distribution for calculating the efficiency of cooling. From the results of numerical analysis, we have obtained the relationship between the cooling efficiency and each design factor.

인체 산화적 DNA손상에 대한 Human Biomonitoring도구로서 Alkaline Comet Assay의 활용 가능성 연구 (Application of the Alkaline Comet Assay for Detecting Oxidative DNA Damage in Human Biomonitoring)

  • 박은주;강명희
    • Journal of Nutrition and Health
    • /
    • 제35권2호
    • /
    • pp.213-222
    • /
    • 2002
  • The alkaline comet assay has been used with increasing popularity to investigate the level of DNA damage in biomonitoring studies within the last decade in Western countries. The purpose of this study was to evaluate the usefulness of the alkaline comet assay as a biomarker of oxidative DNA damage for monitoring in the Korean population, and also to evaluate the effect of nutritional status and lifestyle factors on H2O2 induced oxidative DNA damage measured by the alkaline comet assay in human lymphocytes. The study population consisted of 61 healthy Korean male volunteers, aged 20-28. Epidemiological background data including dietary habits, smoking habits and anthropometrical measurements were collected through personal interviews. After blood collection, the comet assay in peripheral lymphocytes and plasma lipids analysis was carried out and the results analyzed. Tail moment (TM) and tail length (TL) of the comet assay were use\ulcorner to measure DNA damage in the lymphocytes of the subjects. Statistically significant (p < 0.05) positive correlations were observed between DNA damage (TM or TL) and smoking habits expressed as cigarettes smoked per day and pack years (r = 0.311 and 0.382 for TM, r = 0.294 and 0.350 for TL, respectively). There were also significant positive correlations between DNA damage parameter and waist-hip ratio. Higher plasma triglyceride levels were associated with increased damage to DNA. There were no correlations between the consumption frequencies of vegetables and DNA damage to the subjects. However, consumption frequencies of fruit and fruit juice intake were inversely associated with the TM and TL. The results indicate that die comet assay is a simple, rapid and sensitive method for detecting lymphocyte DNA damage induced by cigarette smoking. Consumption of fruit or fruit juices could potentiall modify the damaged DNA in the human peripheral lymphocytes of young Korean men.

초기 흰쥐 배아의 발생단계에 있어서의 Alkaline Phosphatase의 활성에 관한 연구 (A Study of Alkaline Phosphatase Activity on the Preimplantation Mouse Embryos)

  • Cho, Wan-Kyoo;Lee, Chung-Choo;Kim, Hee-Kwon
    • 한국동물학회지
    • /
    • 제27권1호
    • /
    • pp.1-12
    • /
    • 1984
  • 생쥐 난자 및 초기 배아의 alkaline phosphatase의 기능을 알아보기 위하여 생화학적인 방법으로 배아의 발생 단계에 따른 효소의 활성도를 측정하였으며, 동 효소의 저해제로 알려진 levamisole이 난자의 성숙분열 및 초기 배아의 발생에 미치는 영향을 관찰하였다. 1. 동 효소의 활성도는 4세포기에서 뚜렷이 나타나며, 각 발생단계에 따른 현저한 변화는 없는 것으로 나타났다. 2. Blastocyst의 alkaline phosphatase의 활성도는 1 mM 및 10 mM의 levamisole에 의해서 각각 40%와 70% 이상 억제되었다. 3. Levamisole은 0.5 mM 이상의 농도에서 난자의 극체 형성을 완전히 억제하였으며, 동일한 농도에서 2세포기 배아 및 morula의 퇴화현상을 일으켰다.

  • PDF

세공충진 음이온 전도성막의 제조 및 이를 이용한 고체알칼리 연료전지 성능 평가 (Pore-filling anion conducting membranes and their cell performance for a solid alkaline fuel cell)

  • 최영우;이미순;박구곤;임성대;양태현;김창수
    • 한국신재생에너지학회:학술대회논문집
    • /
    • 한국신재생에너지학회 2010년도 춘계학술대회 초록집
    • /
    • pp.129.2-129.2
    • /
    • 2010
  • AEM which were used for solid alkaline fuel cell(SAFC) were prepared by photo polymerization in method pore-filling with various quaternary ammonium cationic monomers and crosslinkers without an amination process. Their specific thermal and chemical properties were characterized through various analyses and the physico-chemical properties of the prepared electrolyte membranes such as swelling behavior, ion exchange capacity and ionic conductivity were also investigated in correlation with the electrolyte composition. The polymer electrolyte membranes prepared in this study have a very wide hydroxyl ion conductivity range of 0.01 - 0.45S/cm depending on the composition ratio of the electrolyte monomer and crosslinking agent used for polymerization. However, the hydroxyl ion conductivity of the membranes was relatively higher at the whole cases than those of commercial products such as A201 membrane of Tokuyama. These pore-filling membranes have also excellent properties such as smaller dimensional affects when swollen in solvents, higher mechanical strength, lowest electrolyte crossover through the membranes, and easier preparation process compared of traditional cast membranes. The prepared membranes were then applied to solid alkaline fuel cell and it was found comparable fuel cell performance to A201 membrane of Tokuyama.

  • PDF