A Study of Alkaline Phosphatase Activity on the Preimplantation Mouse Embryos

초기 흰쥐 배아의 발생단계에 있어서의 Alkaline Phosphatase의 활성에 관한 연구

In order to investigate the alkaline phosphtase activities in the mouse oocytes in matuation and preimplantation embryos in developing in culture, the enzyme activities were measured by means of biochemical method. The in vitro effect of levamisole which is known as an inhibitor of the lakaline phosphatase was also observed on the oocyte in maturation and the embryos in early embryogenesis. The results obtained were as follows: The enzyme activity was not detected in the embryos unitl the stage of 4-cell, but it appeared first in the 4-cell embryos and the level of the activity was steady through up to the blastocyst. Levamisole inhibited the alkaline phosphatase activity in the blastocyst, and the activity decreased by almost 70% at 10 mM and 50% at 1 mM as compared with the control. In addition, levamisole inhibited completely the formation of polar body by the oocytes. and induced degeneration of the preimplantation embryos at the dose of 0.5 mM or higher.

생쥐 난자 및 초기 배아의 alkaline phosphatase의 기능을 알아보기 위하여 생화학적인 방법으로 배아의 발생 단계에 따른 효소의 활성도를 측정하였으며, 동 효소의 저해제로 알려진 levamisole이 난자의 성숙분열 및 초기 배아의 발생에 미치는 영향을 관찰하였다. 1. 동 효소의 활성도는 4세포기에서 뚜렷이 나타나며, 각 발생단계에 따른 현저한 변화는 없는 것으로 나타났다. 2. Blastocyst의 alkaline phosphatase의 활성도는 1 mM 및 10 mM의 levamisole에 의해서 각각 40%와 70% 이상 억제되었다. 3. Levamisole은 0.5 mM 이상의 농도에서 난자의 극체 형성을 완전히 억제하였으며, 동일한 농도에서 2세포기 배아 및 morula의 퇴화현상을 일으켰다.