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Application of the Alkaline Comet Assay for Detecting Oxidative DNA Damage in Human Biomonitoring  

박은주 (경남대학교 자연과학대학 생명과학부)
강명희 (한남대학교 이과대학 식품영양학과)
Publication Information
Journal of Nutrition and Health / v.35, no.2, 2002 , pp. 213-222 More about this Journal
Abstract
The alkaline comet assay has been used with increasing popularity to investigate the level of DNA damage in biomonitoring studies within the last decade in Western countries. The purpose of this study was to evaluate the usefulness of the alkaline comet assay as a biomarker of oxidative DNA damage for monitoring in the Korean population, and also to evaluate the effect of nutritional status and lifestyle factors on H2O2 induced oxidative DNA damage measured by the alkaline comet assay in human lymphocytes. The study population consisted of 61 healthy Korean male volunteers, aged 20-28. Epidemiological background data including dietary habits, smoking habits and anthropometrical measurements were collected through personal interviews. After blood collection, the comet assay in peripheral lymphocytes and plasma lipids analysis was carried out and the results analyzed. Tail moment (TM) and tail length (TL) of the comet assay were use\ulcorner to measure DNA damage in the lymphocytes of the subjects. Statistically significant (p < 0.05) positive correlations were observed between DNA damage (TM or TL) and smoking habits expressed as cigarettes smoked per day and pack years (r = 0.311 and 0.382 for TM, r = 0.294 and 0.350 for TL, respectively). There were also significant positive correlations between DNA damage parameter and waist-hip ratio. Higher plasma triglyceride levels were associated with increased damage to DNA. There were no correlations between the consumption frequencies of vegetables and DNA damage to the subjects. However, consumption frequencies of fruit and fruit juice intake were inversely associated with the TM and TL. The results indicate that die comet assay is a simple, rapid and sensitive method for detecting lymphocyte DNA damage induced by cigarette smoking. Consumption of fruit or fruit juices could potentiall modify the damaged DNA in the human peripheral lymphocytes of young Korean men.
Keywords
human biomonitoring; alkaline comet assay; oxidative DNA damages; cigarette smoking; WHR; plasma triglyceride; fruit consumption;
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Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Microgel electrophoresis assay (comet assay) and SCE analysis in human lymphocytes from 100 normal subjects /
[ Betti C;Davini T;Giannessi L;Loprieno N;Barale R ] / Mutat Res   DOI
2 The effect of smoking and eating habits on DNA damage in Indian population as measured in the Comet assay /
[ Dhawan A;Mathur N;Seth PK ] / Mutat Res   DOI
3 Interaction of lipid peroxidation products with DNA;a review /
[ Vaca CE;Wihelm J;Harms-Ringdahl M ] / Mutat Res
4 /
[ United States Surgeon General ] / Reducing the Health Consequences of Smoking;25 Years of Progeress
5 Single cell gel electrophoresis assay;a new technique for human biomonitoring studies /
[ Kassie F;Parzefall W;Knasmueller S ] / Mutat Res   DOI   ScienceOn
6 Comparative studies by Comet test and SCE analysis in human lymphocytes from 200 healthy subjects /
[ Betti C;Davini T;Giannessi L;Loprieno N;Barale R ] / Mutat Res
7 The comet assay;a comprehensive review /
[ Fairbairn DW;Olive PL;O'Neill KL ] / Mutat Res   DOI   ScienceOn
8 DNA damage as an intermediate biomarker in intervention studies /
[ Santella RM ] / PSEMB
9 Oxidants, antioxidants and the degenerative diseases of aging /
[ Ames BN;Shigenaga MK;Hagen TM ] / Proc Natl Acad Sci USA   DOI   ScienceOn
10 Increased lymphocyte DNA stand breaks in rubber workers /
[ Zhu CQ;Lam TH;Jiang CQ;Wei BX;Xu QR;Chen YH ] / Mutat Res   DOI   ScienceOn
11 Does tomato consumption effectively increase the resistance of lymphocyte DNA to oxidative damage? /
[ Riso P;Pinder A;Santangelo A;Porrini M ] / Am J Clin Nutr
12 Single cell gel electrophoresis on peripheral blood leukocytes of patients with oral squamous cell carcinoma /
[ Rao GV;Kumar GS;Ahuja YR ] / J Oral Pathol Med   DOI   ScienceOn
13 Effect of antioxidants and chelating agents on 1,2,4-benzenetriol-induced DNA damage in HL-60 cells analysed by alkaline comet assay /
[ Kim SJ;Chung HW ] / Environmental Mutagens & Carcinogens
14 Nitric oxide production by coronary condectance and resistance vesseles in hypercholesterolemic patients /
[ Shiode N;Nakayama K;Morishima N;Yamagata T;Matsuura H;Kajiyama G ] / Am Heart J   DOI   ScienceOn
15 Oxidants in cigarette smoke;radicals, hydrogen peroxide, peroxynitrate, and peroxynitrite /
[ Pryor WA;Stone K ] / Ann NY Acad Sci   DOI
16 The single cell microgel electrophoresis assay (comet assay);technical aspects and applications /
[ Ross GM;McMillan IJ;Wilcox P;Collins AR ] / Mutat Res   DOI   ScienceOn
17 Alkaline single cell gel electrophoresis and human biomonitoring for genotoxicity;a study on subjects with residential exposure to radon /
[ Hellman B;Friis L;Vaghef H;Edling C ] / Mutat Res   DOI   ScienceOn
18 Comet assay application in environmental monitoring;DNA damage in human leukocytes and plant cells in comparison with bacterial and yeast tests /
[ Poli P;buschini A;Restivo FM;Ficarelli A;Cassoni F;Ferrero I;Rossi C ] / Mutagenesis   DOI   ScienceOn
19 Determination of DNA damage induced by oxidative stress in hyperlipidemic patients /
[ Harangi M;Remenyik E;Seres I;Varga Z;Katona E;Paragh G ] / Mutat Res   DOI   ScienceOn
20 The single cell gel electrophoresis essay (comet assay);a European review /
[ McKelvey-Martin VJ;Green MHL;Schmezer P;Pool-Zobel BL;DeM대 MP;Collins A ] / Mutat Res   DOI   ScienceOn
21 Consumption of vegetables reduces genetic damage in human;first results of a human intervention trial with carotenoid-rich foods /
[ Pool-Zobel BL;Bub A;Muller H;Wollowski I;Rechkemmer G ] / Carcinogenesis   DOI   ScienceOn
22 Cancer risk and oxidative DNA damage in men /
[ Loft S;Poulsen HE ] / J Mol Med   DOI   ScienceOn
23 DNA damage in exfoliated buccal cells of smokers assessed by the single cell gel electrophoresis assay /
[ Rojas E;Valverde M;Sordo M;Ostrosky-Wegman P ] / Mutration Research   DOI
24 Role of oxidative DNA damage in cancer initiation and promotion /
[ Poulsen HE;Preme H;Loft S ] / Eur J Cancer Prev
25 Effects of smoking and aging on oxidative DNA damage of human lymphocytes /
[ Piperakis SM;Visvardis EE;Sagnou M;Tassiou AM ] / Carcinogenesis   DOI   ScienceOn
26 Hypercholesterolemia increases endothelial superoxide production /
[ Ohara Y;Peterson TE;Harrison DG ] / J Clin Invest   DOI   ScienceOn
27 The comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confouding factors /
[ Moller P;Knusdsen LE;Loft S;Wallin H ] / Cancer Epidemiol Biomarkers Prev
28 Epidemiological studies of the relationship between carcinogenicity and DNA damage /
[ Kaldor J;Day NE ] / IARC Sci Publ
29 Effect of antioxidant vitamins on the transient impairment of endothelium-dependent brachial artery vasoactivity following a single high-fat meal /
[ Plotnick GD;Gorretti MC;Vogel RA ] / JAMA   DOI   ScienceOn
30 Alkaline single-cell gel electorphoresis and human biomonitoring for genotoxicity;a pilot study on breast cancer patients undergoing chemotherapy including cyclophosphamide /
[ Vaghef H;Nygren P;Edling C;Bergh J;Hellman B ] / Mutat Res   DOI
31 Antigenotoxic effect of dominant bacterial isolates from kimchi in vitro /
[ Choi JW;Park JH;Ji ST;Chio OB;Shin HK ] / Korean J Food Sci Technol
32 Estimation of the concentration of low-density lipoprotein cholesterol in plasma, withour use of the preparative ultracentrifuge /
[ Friedwald WT;Levy RI;Fredrickson DS ] / Clin Chem
33 Detection of DNA damage in studies on cancer etiology and prevention /
[ Wogan GN ] / IARC Sci Publ
34 Microgel electrophoretic study of radiation-induced DNA damages in individual mammalian cells /
[ Ostling O;Johansom KJ ] / Biochem Biophys Res Commun   DOI   ScienceOn
35 Cigarette smoke-induced DNA damage in cultured human lung cells;role of hydroxyl radicals and endonuclease activation /
[ Leanderson P;Tagesson C ] / Chem Biol Interact   DOI   ScienceOn
36 Effects of air pollution and smoking on DNA damage of human lymphocytes /
[ Piperakis SM;Petrakou E;Tsilimigaki S ] / Environ Mol Mutagen   DOI   ScienceOn
37 Oxidants in cigarette smoke;radicals, hydrogen peroxide, peroxynitrate /
[ Pryor W;Stone K ] / Ann NY Acad Sci   DOI
38 Consumption of vegetables and fruits and urothelial cancer incidence;a prospective study /
[ Zeegers MP;Goldbohm RA;van Den Brandt PA ] / Cancer Epidemiol Biomarkers Prev
39 /
[ Gutteridge JMC;Halliwell B ] / Antioxidants in Nutrition, Health, and Disease
40 Fruit and vegetable consumption in the prevention of cancer;an update /
[ Terry P;Terry JB;Wolk A ] / J Int Med   DOI   ScienceOn
41 Comparative studies by Comet test and SCE analysis in human lymphocytes from 100 normal subjects /
[ Betti C;Davini T;Giannessi L;Lopreno N;Barale R ] / Mutat Res   DOI
42 Application of the comet assay for DNA damage in worders exposed to chronic low-dose irradiation.Ⅰ. Standard breakage /
[ Wojewodzka M;Kruszewski M;Jwanenko T;Collins AR;Szumiel I ] / Mutat Res   DOI
43 Validation of biomarkers as early predictors of disease /
[ Bonassi S;Neri M;Puntoni R ] / Mutat Res
44 Studies in the distribution of body fat.Ⅲ. ;Effects of cigarette smoking /
[ Shimokata H;Muller DC;Andres R ] / JAMA   DOI   ScienceOn
45 A simple technique for quantitation of low levels of DNA damage in individual cells /
[ Singh PN;McCoy MT;Tice RR;Schneider EL ] / Exp Cell Res   DOI   ScienceOn
46 Peroxynitrite modification of low-density lipoprotein leads to recognition by the macrophage scavenger receptor /
[ Graham A;Hogg N;Kalyanaraman B;O'Leary V;Darley-Usmar V;Moncada S ] / FEBS Lett   DOI   ScienceOn
47 Evaluation of DNA damage in leukocytes of ex-smokers by single cell gel electrophoresis /
[ Frenzilli G;Betti C;Davini T;Desideri M;Fornai E;Giannessi L;Maggiorelli F;Paoletti P;Barale R ] / Mutat Res   DOI
48 Single cell gel electrophoresis assay;methodology and appliacations /
[ Rojas E;Lopez MC;Valverde M ] / J Chromatography   DOI
49 Human sperm DNA integrity assessed by the comet assay and ELISA assays /
[ Hughes CM;McKelvey-Martin VJ;Lewis SEM ] / Mutagenesis   DOI   ScienceOn
50 Comet assay in human biomonitoring studies;reliability, validation, and applications /
[ Collins A;Dusinska M;Franklin M;somorovska M;Petrovska H;Duthie S;Fillion L;Panayiotidis M;Raslova K;Vaughan N ] / Environ Mol Mutagen   DOI   ScienceOn
51 Kiwifruit protects against oxidative DNA damage in human cells and in vitro /
[ Collins BH;Horska A;Hotten PM;Riddoch C;Collins AR ] / Nutr Cancer   DOI   ScienceOn