• ALGAE
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• v.28 no.4
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• pp.343-354
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• 2013

To explore the interactions between the mixotrophic dinoflagellate Biecheleria cincta (previously Woloszynskia cincta) and heterotrophic protists, we investigated whether the common heterotrophic dinoflagellates Gyrodinium dominans, Gyrodinium moestrupii, Gyrodinium spirale, Oxyrrhis marina, and Polykrikos kofoidii, and the ciliate Strobilidium sp. were able to feed on B. cincta. We also measured growth and ingestion rates of O. marina and Strobilidium sp. on B. cincta as a function of prey concentration. In addition, these rates were measured for other predators at single prey concentrations at which the growth and ingestion rates of O. marina and Strobilidium sp. were saturated. All grazers tested in the present study were able to feed on B. cincta. B. cincta clearly supported positive growth of O. marina, G. dominans, and Strobilidium sp., but it did not support that of G. moestrupii, G. spirale, and P. kofoidii. The maximum growth rates of Strobilidium sp. and O. marina on B. cincta (0.91 and 0.49 $d^{-1}$, respectively) were much higher than that of G. dominans (0.07 $d^{-1}$). With increasing the mean prey concentration, the specific growth rates of O. marina and Strobilidium sp. on B. cincta increased, but either became saturated or slowly increased. The maximum ingestion rate of Strobilidium sp. (1.60 ng C $predator^{-1}\;d^{-1}$) was much higher than that of P. kofoidii and O. marina (0.55 and 0.34 ng C $predator^{-1}\;d^{-1}$) on B. cincta. The results of the present study suggest that O. marina and Strobilidium sp. are effective protistan grazers of B. cincta.

• ALGAE
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• v.29 no.2
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• pp.153-163
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• 2014

Mesodinium rubrum is a cosmopolitan ciliate that often causes red tides. Predation by heterotrophic protists is a critical factor that affects the population dynamics of red tide species. However, there have been few studies on protistan predators feeding on M. rubrum. To investigate heterotrophic protists grazing on M. rubrum, we tested whether the heterotrophic dinoflagellates Gyrodiniellum shiwhaense, Gyrodinium dominans, Gyrodinium spirale, Luciella masanensis, Oblea rotunda, Oxyrrhis marina, Pfiesteria piscicida, Polykrikos kofoidii, Protoperidinium bipes, and Stoeckeria algicida, and the ciliate Strombidium sp. preyed on M. rubrum. G. dominans, L. masanensis, O. rotunda, P. kofoidii, and Strombidium sp. preyed on M. rubrum. However, only G. dominans had a positive growth feeding on M. rubrum. The growth and ingestion rates of G. dominans on M. rubrum increased rapidly with increasing mean prey concentration < $321ngCmL^{-1}$, but became saturated or slowly at higher concentrations. The maximum growth rate of G. dominans on M. rubrum was $0.48d^{-1}$, while the maximum ingestion rate was 0.55 ng C $predator^{-1}d^{-1}$. The grazing coefficients by G. dominans on populations of M. rubrum were up to $0.236h^{-1}$. Thus, G. dominans may sometimes have a considerable grazing impact on populations of M. rubrum.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.11
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• pp.4649-4655
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• 2010

The aim of this study was to investigate the effect of flowing the industrial wastewater effluents into the K lake which is located in the A city on the eutrophication. The lake was modelled as a continuous stirred-tank reactor. Phosphorus and Chl-a concentration in the lake were calculated to $0.29\;/m^3$과 $4.0\;g/m^3$ at steady state, respectively. Those simulated concentrations were very close to the monitored mean concentration of the lake, indicating that the simulation could be used a tool for characterizing the lake. The non-steady state concentrations of the phosphorus and Chl-a were proposed as a function of time as well. Phosphorus loading ($L_p$) and depth to retention time ratio ($H/{\tau}w$) was calculated in order to analyze the current state of eutrophication. We proposed a strategy to change the lake from eutrophic to permissible oligotrophic state using a graph consisting of two variables, $L_p$ and $H/{\tau}w$.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.478-478
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• 2016

4대강 다기능 보 건설 이후 보 내에서 발생하는 녹조를 효과적으로 저감시키기 위해 다양한 방법들이 시도되고 있으며, 댐-보 및 보-보간 연계운영 기법은 수량을 이용하여 대규모 구간에 걸쳐 효과적으로 조류를 저감시킬 수 있어 최근 주목을 받고 있다. 본 연구에서는 영산강 수계 승촌보와 죽산보를 대상으로 조류저감을 위한 다기능보 연계 운영 시나리오에 대해 2차원 모델링을 이용하여 효과를 분석하고 향후 조류저감을 위한 가동보 운영방안 수립에 적용하고자 수행되었다. 시뮬레이션 기간은 2014년 7월 1일부터 7월 15일까지 남조류가 다량으로 발생하였던 시기를 모의기간으로 하였다. 분석시나리오는 최근 낙동강에서 적용된 바 있는 pulse형 방류를 실시하여 수체내에 유속과 난류를 증가시키되 승촌보와 죽산보를 연계하여 순차적인 방류와 담수를 반복하는 형태를 가정하였다. 또한 방류 규모에 따라 수위 저하범위를 0.3~2 m로 차등 적용하여 조류저감 효과를 예측하였다. 예측결과 소수력 발전방류를 이용한 pulse형 방류 연계운영 CASE에서는 최대 승촌보 3.8% 죽산보 8.4%의 저감 효과가 예측되었다. 반면 소수력과 수문방류를 동시에 이용하여 수위 저하범위를 크게 적용하는 CASE에서는 승촌보에서 최대 12.5%, 죽산보 4.7%의 저감율로 방류규모 증가에도 불구하고 죽산보의 저감율은 증가하지 않는 결과를 나타내었다. 이는 상대적으로 저수량이 큰 죽산보에서 큰 폭으로 수위를 저하시킬 경우 수위회복 기간이 길어지고 이 시기에 조류가 성장할 수 있는 조건을 제공하기 때문인 것으로 판단된다. 따라서 조류의 발생 규모와 공간적 분포, 보별 유입지류 유입특성과 저수량 등 조류발생에 관련된 다양한 인자를 복합적으로 고려하여 효율적인 운영방안이 수립되어야 할 것으로 판단된다.

• Journal of Korean Society on Water Environment
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• v.27 no.5
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• pp.670-679
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• 2011

Chlorophyll-a is a major water quality indicator for an algal bloom in streams and lakes. The purpose of the study is to estimate chlorophyll-a concentration in tributaries of the Seonakdonggang by an artificial neural network (ANN). As the tributaries are ungauged streams, a watershed runoff and quality model was used to simulate water quality parameters. The tributary watersheds include urban area and thus Storm Water Management Model (SWMM) was used to simulate TN, TP, BOD, COD, and SS. SWMM, however, can not simulate chlorophyll-a. The chlorophyll-a series data from the tributaries were estimated by the ANN and the simulation results of water quality parameters using SWMM. An assumption used is as follows: the relation between water quality parameters and chlorophyll-a in the tributaries of the Seonakdonggang would be similar to that in the mainstream of the Seonakdonggang. On the assumption, the measurement data of water quality and chlorophyll-a in the mainstream of the Seonakdonggang were used as the learning data of the ANN. Through the sensitivity analysis, the learning data combination of water quality parameters was determined. Finally, chlorophyll-a series were estimated for tributaries of the Seonakdonggang by the ANN and TN, TP, BOD, COD, and temperature data from those streams. The relative errors between the estimated and measured chlorophyll-a were approximately 40 ~ 50%. Though the errors are somewhat large, the estimation process for chlorophyll-a may be useful in ungauged streams.

• ALGAE
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• v.36 no.4
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• pp.299-314
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• 2021

Some species in the dinoflagellate genus Alexandrium are bioluminescent. Of the 33 formally described Alexandrium species, the bioluminescence capability of only nine species have been tested, and eight have been reported to be bioluminescent. The present study investigated the bioluminescence capability of seven Alexandrium species that had not been tested. Alexandrium mediterraneum, A. pohangense, and A. tamutum were bioluminescent, but A. andersonii, A. hiranoi, A. insuetum, and A. pseudogonyaulax were not. We also measured the bioluminescent intensity of A. affine, A. fraterculus, A. mediterraneum, A. ostenfeldii, A. pacificum, A. pohangense, A. tamarense, and A. tamutum. The mean 200-second-integrated bioluminescence intensity per cell ranged from 0.02 to 32.2 × 10⁴ relative luminescence unit per cell (RLU cell^-1), and the mean maximum bioluminescence intensity per cell per second (BL_Max) ranged from 0.01 to 10.3 × 10⁴ RLU cell^-1 s^-1. BL_Max was significantly correlated with the maximum growth rates of Alexandrium species, except for A. tamarense. A phylogenetic tree based on large subunit ribosomal DNA (LSU rDNA) showed that the bioluminescent species A. affine, A. catenella, A. fraterculus, A. mediterraneum, A. pacificum, and A. tamarense formed a large clade. However, the toxicity or mixotrophic capability of these species was split. Thus, their bioluminescence capability in this clade was more consistent than their toxicity or mixotrophic capability. Phylogenetic trees based on LSU rDNA and the luciferase gene of Alexandrium were consistent except for A. pohangense. The results of the present study can provide a basis for understanding the interspecific diversity in bioluminescence of Alexandrium.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.56-56
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• 2023

남양호는 농업용수 공급을 위해 건설된 하구 담수호로 과도한 영양물질 축적으로 인해 매년 여름 녹조류가 번성한다. 따라서 본 연구에서는 조류발생 특성을 분석하고자 식물성 플랑크톤 및 관련 분해 산물에 의해 고유 광학특성을 가지고 있는 Chlorophyll-a(Chl-a)의 추정을 통한 녹조 발생을 파악하고자 Sentinel-2 Multi Spectral Image(MSI)의 원격 반사율 광학 스펙트럼을 사용하였다. Chl-a 추정알고리즘 개발을 위하여 Sentinel-2 A, B의 교차 방문주기인 5일 간격에 맞추어 현장수질자료(2022년: 27회 2023년: 27회)를 측정하였다. Chl-a 농도는 EXO-YSI를이용하여 측정하였으며 해당기간동안 9.4 ~ 127.1 mg/L의 범위를 보였으며, Sentine-2 자료는 A, B자료에서 B1(443 nm) ~ B8A(865 nm)파장의 값을 기상조건(구름, 안개, 강수)을 고려하여 현장수질측정 위치에서 반사도를 추출하였다. 입력자료는 대기 및 방사영향을 고려해 반사도 간의 비율자료와 선행연구에서 활용된 반사도를 활용하였으며 알고리즘은 다중선형회귀분석(Multi Linear Regression Model)과 Random Forest를 활용하였다. MLR의 경우 결정계수(R2)가 학습 및 검증에서 각각 0.68, 0.59의 성능을 보였으며, RF의 경우 각각 0.94, 0.85의 성능을 보였다. 해당알고리즘으로 생성된 Chl-a 시공간농도 자료는 담수호내 조류발생 특성을 분석하고 효율적 조류관리 및 대처에 활용될 것으로 판단된다.

• ALGAE
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• v.38 no.2
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• pp.111-126
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• 2023

Mixotrophic dinoflagellates act as primary producers, prey, and predators in marine planktonic food webs, whereas exclusively autotrophic dinoflagellates are primary producers and prey. Species of the dinoflagellate genus Scrippsiella are commonly found in marine ecosystems and sometimes cause harmful red tides. Among the 28 formally described Scrippsiella species, S. acuminata has been found to be mixotrophic and two unidentified species have been found to be mixotrophic. To determine whether the other species in this genus are similarly mixotrophic, the mixotrophic ability of S. donghaiensis SDGJ1703, S. lachrymosa SLBS1703, S. masanensis SSMS0908, S. plana SSSH1009A, and S. ramonii VGO1053 was explored using 15 potential prey items, including 2-㎛ fluorescently labeled microspheres (FLM) and heterotrophic bacteria (FLB), the cyanobacterium Synechococcus sp., and various microalgal prey species. The ability of S. acuminata to feed on FLM and FLB was also investigated. We found that S. donghaiensis, S. lachrymosa, S. masanensis, S. plana, and S. ramonii did not feed on any potential prey tested in this study, indicating a lack of mixotrophy. However, S. acuminata fed on both FLM and FLB, confirming its mixotrophic ability. These results lowered the proportion of mixotrophic species relative to the total number of tested Scrippsiella species for mixotrophy from 100% to 29-38%. Owing to its mixotrophic ability, S. acuminata occupies an ecological niche that is distinct from that of S. donghaiensis, S. lachrymosa, S. masanensis, S. plana, and S. ramonii.

• Journal of Korean Society on Water Environment
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• v.40 no.2
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• pp.79-88
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• 2024

This study employed field measurements and biogeochemical analysis to examine the effects of seasonal conditions (e.g., temperature and precipitation) and human intervention (e.g., dam or weir construction) on the chemical composition of dissolved organic matter, flocculation kinetics of suspended particulate matter, and formation of the fluid mud layer on riverbeds. The results indicated that a water environment with a substantial amount of biopolymers offered favorable conditions for flocculation kinetics during an algal bloom period in summer; a thick fluid mud layer was found to be predominated with cohesive materials during this period. However, after high rainfall, a substantial influx of terrigenous humic substances led to enhanced stabilization of the particulate matter, thereby decreasing flocculation and deposition, and the reduced biopolymer composition served to weaken the erosion resistance of the fluid mud on the riverbed. Moreover, a high-turbulence condition disaggregated the flocs and the fluid mud layer and resuspended the suspended particulate matter in the water column. This study demonstrates the mutual relationship that exists between biogeochemistry, flocculation kinetics, and the formation of the fluid mud layer on the riverine area during different seasons and under varying hydrological conditions. These findings are expected to eventually help inform the more optimal management of water resources, which is an urgent task in the face of anthropogenic stressors and climate change.

• ALGAE
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• v.39 no.1
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• pp.1-16
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• 2024

Many dinoflagellates produce bioluminescence. To estimate the intensity of bioluminescence produced by populations of the heterotrophic dinoflagellates Noctiluca scintillans and Polykrikos kofoidii and autotrophic dinoflagellate Alexandrium mediterraneum in Korean waters, we measured cellular bioluminescence intensity as a function of water temperature and calculated population bioluminescence intensity with cell abundances and water temperature. The mean 200-second-integrated bioluminescence intensity per cell (BL_cell) of N. scintillans satiated with the chlorophyte Dunaliella salina decreased continuously with increasing water temperature from 5 to 25℃. However, the BL_cell of P. kofoidii satiated with the mixotrophic dinoflagellate Alexandrium minutum continuously increased from 5 to 15℃ but decreased at temperatures exceeding this (to 30℃). Similarly, the BL_cell of A. mediterraneum continuously increased from 10 to 20℃ but decreased between 20 and 30℃. The difference between highest and lowest BL_cell of N. scintillans, P. kofoidii, and A. mediterraneum at the tested water temperatures was 3.5, 11.8, and 21.0 times, respectively, indicating that water temperature clearly affected BL_cell. The highest estimated population bioluminescence intensity (BL_popul) of N. scintillans in Korean waters in 1998-2022 was 4.22 × 10¹³ relative light unit per liter (RLU L^-1), which was 1,850 and 554,000 times greater than that of P. kofoidii and A. mediterraneum, respectively. This indicates that N. scintillans populations produced much brighter bioluminescence in Korean waters than the populations of P. kofoidii or A. mediterraneum.