• 식물병연구
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• 제9권1호
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• pp.1-9
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• 2003

The occurrence of potato(Sotanum tuberosum) viral diseases caused by Potato virus X(PVX), Potato virus Y (PVY), Potato leafroll virus(PLRV), Potato vims S(PVS), Potato virus M(PVM), Potato virus A(PVA), Potato virus T(PVT), Alfalfa mosic virus(AIMV), Tobacco mosic virus(TMV), Potato mop top virus(PMTV) Tobacco rattle virus(TRV) and Potato spindle tuber viroid(PSTVd), potato witches' broom phytoplasma, have been identified so far in Korea. Major viral diseases such as PVX, PVY and PLRV had been studied more deeply, however, the others are just identified and only partially characterized since the first study on the relation between PVX nucleic acid and virus protein by Kim in 1961. The most studies on potato viral diseases are mainly focused on the problems of seed potato production. The National Alpine Agricultural Experiment Station(NAAES), since it began its activities in 1961, has given special attention to this problem by doing studies to identify, characterize and control potato virus diseases. This effort resulted in the development of new potato virus detection methods as a basis for elaborating new method of control, such as the production of seed potato free of virus and the selection of new virus-resistant transgenic potatoes. The further studies of potato viral diseases required would be fallowings: the continuous monitoring for the occurrence of identified or not identified potato viruses in Korea, the isolation of resistant viral genes, the development of control method for the non-persistently transmitted viruses like PVY, special vectors such as nematode and fungus transmitted viruses, TRV and PMTV and the development of control methods against potato viral diseases by viral cross protection, therapy, transgenic plant, and the use of the agents or molecules, such as virus inhibitors and antiviral proteins, etc., blocking viral replication.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.425-441
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• 2010

Forages are the backbone of sustainable agriculture. They includes a wide variety of plant species ranging from grasses, such as tall fescue and bermudagrass, to herbaceous legumes, such as alfalfa and white clover. Abiotic stresses, especially salinity, drought, temperature extremes, high photon irradiance, and levels of inorganic solutes, are the limiting factors in the growth and productivity of major cultivated forage crops. Given the great complexity of forage species and the associated difficulties encountered in traditional breeding methods, the potential from molecular breeding in improving forage crops has been recognized. Plant engineering strategies for abiotic stress tolerance largely rely on the gene expression for enzymes involved in pathways leading to the synthesis of functional and structural metabolites, proteins that confer stress tolerance, or proteins in signaling and regulatory pathways. Genetic engineering allows researchers to control timing, tissue-specificity, and expression level for optimal function of the introduced genes. Thus, the use of either a constitutive or stress-inducible promoter may be useful in certain cases. In this review, we summarize the recent progress made towards the development of transgenic forage plants with improved tolerance to abiotic stresses.

• 한국유기농업학회지
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• 제7권1호
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• pp.79-90
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• 1998

제주지역에서 前作物로 綠肥作物을 재배하여 破碎$\cdot$耕耘하고 폴리에칠렌 투명비닐로 1997년 8월 5일부터 8월 29일까지 太陽熱土壤消毒후 가을감자를 재배한 결과를 요약하면 다음과 같다. 멀칭기간중의 토양깊이 10cm에서 최고지원은 $42^{\circ}C$였다. 綠肥作物栽培 및 멀칭에 의해 토양의 pH 및 有機物含量은 높아졌다. 前作物로 재배한 綠肥作物의 生體收量은 sudangrass 交雜種, 콩, 알팔파 순으로 많앙Tejs 반면 멀칭 太陽熱土壤消毒후 가을감자의 地上部生體重, 塊莖收量 등의 생육형질은 콩 + 가을감자재배구가 가장 양호한 것으로 나타났다. 이상의 결과로 볼 때 여름철 고온기에 綠肥作物栽培후 비닐 피복 太陽熱消毒에 의해 작물의 생육형질이 향상될 뿐만 아니라 土壤消毒의 가능성도 있을 것으로 생각된다.

• 한국초지조사료학회지
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• 제41권3호
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• pp.176-182
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• 2021

Ensiling is the most preferred technology to preserve the silage quality with high nutrients by the presence of lactic acid bacteria. In this study, lactic acid bacteria RJ1 and S22 were used to make the silages from different leguminous plants such as alfalfa, hairy vetch and red clover. Experimental groups were divided into control and LAB inoculated groups. LAB inoculated group; all legumes treated with a mixture of RJ1 and S22 and made an anaerobic condition for 45d. Without the addition of LAB considered the control group. The results showed that the lactic acid content was higher in all silages in response to LAB treatment and acetic acid content was slightly increased except red clover by LAB compared to control silages. A poor silage quality marker butyric acid was reduced all legume silages in response to LAB inoculation than control silages. The organic acid is closely associated with microbial population experimental silages. We noted that higher LAB and lower yeast were found in the silage in response to LAB treatment. The contents of crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), and total digestible nutrient (TDN) were not altered significantly between control and LAB treated silages. Overall data suggested that the inclusion of additional LAB potentially enhance the silage quality and preserved the nutrients for long period.

• Asian-Australasian Journal of Animal Sciences
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• 제32권9호
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• pp.1439-1447
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• 2019

Objective: The aim of this study was to evaluate the effect of dietary incorporation of tanninrich woody species on meat oxidative stability, carcass traits and meat quality in goats. Methods: Two tannin-rich species were tested using a three-treatments feeding trial, where treatments consisted of: Larrea divaricata and Acacia aroma both at 12.5% in dry matter basis of the diet and a control diet (alfalfa hay). All feeding diets were iso-protein and iso-energy. Carcass conformation, carcass compactness, carcass fatness and subcutaneous fat deposition were evaluated. Intake, liveweigh, Longissimus thoracis et lumborum muscles of goats were analyzed in order to evaluate quality parameters such as pH value, instrumental color evaluation, water holding capacity, total phenolic content, antioxidant activity, meat oxidative stability and fatty acid profiles in meat. Results: Feed intake, liveweight gain, carcass, and meat traits did not differ among treatments. Changes in meat lipid profile among treatments were observed for oleic and elaidic acid contents. Meat total phenolic content and antioxidant activity did not differ among treatments; although, meat oxidative status after storage at room temperature, as well as under refrigerated and frozen conditions were different between control and both supplemented groups. Conclusion: The inclusion of Acacia aroma and Larrea divaricata leaves in goat diet enhanced meat oxidative stability. Modulation of the ruminal biohydrogenation of fatty acids produced by condensed tannins of these plant species need to be further investigated.

• Journal of Animal Science and Technology
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• 제63권6호
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• pp.1433-1442
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• 2021

In this study, Saccharomyces cerevisiae culture fluid (SCCF) has been added to a diet of lactating dairy cows to attempt to improve the ruminal fermentation and potentially increase the dry matter intake (DMI) and milk yield. This study was conducted to investigate the effects of SCCF on the milk yield and blood biochemistry in lactating cows during the summer. Twenty-four Holstein dairy cows were randomly assigned to one of four treatments: (1) total mixed ration (TMR-1) (Control); (2) TMR-1 supplemented with SCCF (T1); (3) TMR-2 (containing alfalfa hay) (T2); and (4) TMR-2 supplemented with SCCF (T3). SCCF (5 ml/head, 2.0×10⁷ CFU/mL) was mixed with TMRs daily before feeding to dairy cows. The mean daily temperature-humidity index (THI) during this trial was 76.92 ± 0.51 on average and ranged from 73.04 to 81.19. For particle size distribution, TMR-2 had a lower >19 mm fraction and a higher 8-9 mm fraction than TMR-1 (p < 0.05). The type of TMR did not influence the DMI, body weight (BW), milk yield and composition, or blood metabolites. The milk yield and composition were not affected by the SCCF supplementation, but somatic cell counts were reduced by feeding SCCF (p < 0.05). Feeding SCCF significantly increased the DMI but did not affect the milk yield of dairy cows. The NEFA concentration was slightly decreased compared to that in the control and T2 groups without SCCF. Feeding a yeast culture of S. cerevisiae may improve the feed intake, milk quality and energy balance of dairy cows under heat stress.

• The Plant Pathology Journal
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• 제38권6호
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• pp.551-571
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• 2022

Xylella fastidiosa is xylem-limited bacterium capable of infecting a wide range of host plants, resulting in Pierce's disease in grapevine, citrus variegated chlorosis, olive quick decline syndrome, peach phony disease, plum leaf scald, alfalfa dwarf, margin necrosis and leaf scorch affecting oleander, coffee, almond, pecan, mulberry, red maple, oak, and other types of cultivated and ornamental plants and forest trees. In the European Union, X. fastidiosa is listed as a quarantine organism. Since its first outbreak in the Apulia region of southern Italy in 2013 where it caused devastating disease on Olea europaea (called olive leaf scorch and quick decline), X. fastidiosa continued to spread and successfully established in some European countries (Corsica and PACA in France, Balearic Islands, Madrid and Comunitat Valenciana in Spain, and Porto in Portugal). The most recent data for Europe indicates that X. fastidiosa is present on 174 hosts, 25 of which were newly identified in 2021 (with further five hosts discovered in other parts of the world in the same year). From the six reported subspecies of X. fastidiosa worldwide, four have been recorded in European countries (fastidiosa, multiplex, pauca, and sandyi). Currently confirmed X. fastidiosa vector species are Philaenus spumarius, Neophilaenus campestris, and Philaenus italosignus, whereby only P. spumarius (which has been identified as the key vector in Apulia, Italy) is also present in Americas. X. fastidiosa control is currently based on pathogen-free propagation plant material, eradication, territory demarcation, and vector control, as well as use of resistant plant cultivars and bactericidal treatments.

• 한국농공학회논문집
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• 제65권4호
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• pp.1-10
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• 2023

As a result of climate change, non-point source pollution (NPS) from farmland with the steep slope during the rainy season is expected to have a significant impact on the water system. This study aimed to evaluate the effect of furrow mulching using alfalfa and PAM (Polyacrylamide) materials for each rainfall event, while considering the load characteristics of NPS. The study was conducted in Wanju-gun, Jeollabuk-do, in 2022, with a testbed that had a slope of 13%, sandy loam soil, and maize crops. The testbed was composed of four plots: bare soil (Bare), No mulching (Cont.), Vegetation mulching (VM), and PAM mulching (PM). Runoff was collected from each rainfall event using a 1/40 sampler and the NPS load was calculated by measuring the concentrations of SS, T-N, T-P, and TOC. During farming season, the reduction efficiency of NPS load was 37.1~59.5% for VM and 38.2~75.7% for PM. The analysis found that VM had a linear regression correlation (R²=0.28~0.86, P-value=0.01~0.1) with elapsed time of application, while PM had a quadratic regression correlation (R²=0.35~0.80, P-value=0.1). These results suggest that the selection of furrow mulch materials and the appropriate application method play a crucial role in reducing non-point pollution in farmland. Therefore, further studies on the time-series reduction effect based on the application method are recommended to develop more effective preemptive reduction technologies.

• Journal of Animal Science and Technology
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• 제46권3호
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• pp.355-372
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• 2004

식물성유 첨가시 배양시간에 따른 지방산염의 형성 정도와 발효성상 및 NDF 소실율에 미치는 영향을 규명하고자 in vitro 시험을 실시 하였다. 기질은 알팔파 건초로 하고 1) oil 무첨가구, 2) 대두유 10%첨가구 및 3) 옥수수유 10%첨가구로 하였으며, oil 첨가 수준은 기질중량 건물 기준으로 첨가하였다. 발효성상 및 NDF 소실율을 위한 시험에서는 120ml serum bottle을 사용하여 3반복 실시하여 pH, $NH_3-N$, VFA농도 및 건물과 NDF 소실율을 분석하엿다. 지방산 분획을 위한 시험에서는 60ml serum bottle을 사용하요 3반복 실시하여 NEFA, EFA 및 FAS의 형성 정도를 분석하였다. pH는 배양시간이 증가함에 따라 유읭적으로 감소하였고, $NH_3-N$ 농도는 유의하게 증가하였다(P<0.0001). 건물 및 NDF 소실율은 처리구, 배양시간 및 oil간 유의하게 차이를 보여주었다(P<0.05). 총 휘발성 지방산 농도는 배양시간이 증가함에 따라 유의하게 증가하였다(P<0.0001). Acetate는 시험구 모두 배양시간이 증가함에 따라 유의적으로 감소하였고(P<0.0001), 대조구에 비해 oil 첨가구가 유의적으로 낮아졌다(P<0.05). Propionate는 시험구 모두 배양시간이 증가함에 따라 유의적으로 증가하였지만(P<0.0001), 처리구 및 oil간 효과는 없었다. Oil 첨가구에서 A/P비율이 유의하게 낮아졌다(P<0.05). EFA는 배양시간이 증가함에 따라 유의하게 감소하였고(P<0.0001), NEFA는 EFA의 가수분해로 인하여 증가하였다. 배양시간 48시간에서는 NEFA가 양이온과 결합하여 FAS의 형성 비율이 유의적으로 증가하였다(P<0.0001). 특히 $C_{18:0)$의 FAS 형성은 배양시간 48시간에서 대두유 및 옥수수유 첨가구에서 각각 12.53 및 15.17mg/g DM으로 0시간에 비해 각각 27.5 및 32.5배 증가하였다(P<0.0001). 배양시간이 증가할수록 칼슘을 포함한 양이온 함량이 높은 알팔파가 분해되면서 양이온이 용해되어 유리된 지방산과 결합하여 지방산염을 형성하게 된다. 이때 불포화지방산보다는 포화지방산이 양이온과 반응하여 지방산염을 형성하기 쉽다. 또한 $C_{16:0}$ 이상의 장쇄지방산이 주로 지방산염을 형성하였다. 이러한 특성 때문에 배양액내에 유리된 지방산이 염을 형성하기 쉽고 oil 첨가시 발생하는 발효성상 및 NDF소실율에 미치는 부의 영향을 최소화 한 것으로 사료된다.

• 농약과학회지
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• 제18권1호
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• pp.26-32
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• 2014

밀양의 자운영 포장에서 친환경농자재의 처리시기(4월 상순, 중순 및 하순) 및 횟수(1, 2회 및 3회)에 따른 알팔파바구미의 방제효과를 평가하였다. 알팔파바구미의 유충, 번데기 및 성충은 2011년보다 2010년에 발생량이 많았으며, 특히 유충의 발생량 차이가 현저하였고, 발생피크도 2011년의 5월 10일보다 5일 빨랐다. 친환경농자재의 알팔파바구미에 대한 평균 방제효과는 2010년에 1회 처리는 4월 상순, 중순 및 하순에 각각 73.6%, 71.9% 및 66.2%이었으며, 2회 처리는 77.1~78.9%로 처리시기조합에 따른 차이가 없었고, 3회 처리는 87.2%였다. 2011년의 평균방제효과는 1회 처리는 4월 상순, 중순 및 하순에 각각 평균 57.9%, 66.8% 및 65.2% 이었으며, 2회 처리는 73.7~76.8%로 처리시기조합에 따른 큰 차이가 없었고, 3회 처리는 평균 82.7% 이었다. 따라서 알팔파바구미의 방제효과는 방제횟수가 증가할수록 증가되었다. 응삼이$^{(R)}$, 왕중왕에코$^{(R)}$ 및 무충지대$^{(R)}$는 알팔파바구미에 효과적인 친환경방제제로 이들 자재의 1회 처리에 의한 방제효과는 화학농약인 ethofenprox 20EC에 비해 4~16% 낮았다. 그러므로 응삼이$^{(R)}$, 왕중왕에코$^{(R)}$ 및 무충지대$^{(R)}$는 알팔파바구미의 친환경적 방제에 효과적으로 사용할 수 있으며, 방제시기 및 횟수는 알팔파바구미의 발생량을 고려하여 달라질 수 있지만, 1회 방제의 경우 발생량이 많은 경우는 4월 상순, 발생량이 적은 경우는 4월 중순이 효과적인 것으로 나타났다.