• 보존과학회지
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• 제35권6호
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• pp.652-663
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• 2019

목조건축문화재의 생물피해 영향을 미치는 야외 부유곰팡이의 연중 변화와 일간 변화를 파악하기 위해서는 포집주기와 측정시간대의 충분한 반영이 필요하다. 본 연구는 2018년 8월부터 2019년 7월까지 1년간 국립문화재연구소 보존과학센터 옥상에서 에어샘플러와 Potato dextrose agar 배지를 이용하여 포집주기는 24절기, 포집시간대는 14시(낮)와 23시(밤)에 포집하였고, 기상요소를 1시간 단위로 수집하였으며, 부유곰팡이의 농도 조사 및 기상요소와의 상관성 분석을 진행하였다. 그 결과, 부유곰팡이 농도는 11월, 가을, 밤에 가장 높았고, 가을, 여름, 겨울, 봄 순으로 높았으며, 강우, 태풍, 황사/미세먼지에 따라 부유곰팡이 농도, 종류, 우점은 달랐다. 부유곰팡이 농도는 강수량, 강수일수, 상대습도와 강한 양의 선형 상관성이 나타났다. 부유곰팡이 농도는 영양원에 있어서는 죽은 식물체의 증가 시기, 기상요소에 있어서는 강우를 포함한 높은 상대습도 조건과 관련이 깊었다.

• 한국환경보건학회지
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• 제33권2호
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• pp.104-114
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• 2007

This study evaluated the airborne concentrations of bacteria, Gram-negative bacteria, and fungi in waiting rooms, wards, and outdoors, according to time and particle size between October 17 and November 28, 2003. The geometric mean number of airborne bacteria was highest in the morning. The more people there were, the higher was the total bacteria concentration. The concentration of fungi was also highest in the morning. Temperature and relative humidity affected the concentrations of fungi significantly (p<0.05). This study found relationships between microorganism concentrations and (actors such as time, place, temperature, humidity, ventilation, and number of people. Therefore, to manage the pollution resulting from airborne microorganisms, each time, place, and environmental factor should be examined periodically, and the number, size, and movement of airborne microorganisms should be evaluated.

• 한국산업보건학회지
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• 제25권2호
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• pp.194-201
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• 2015

Objectives: The purpose of this study is to assess the exposure level to dust and airborne microorganisms among employed workers in a clothing shopping center. Materials and Methods: On-site investigation of a clothing shopping center was performed between October and November 2012. The hazardous substances measured in this study are particulate matter(Total dust, respirable dust) and airborne microorganisms (Total airborne bacteria, total airborne fungi). Results: The highest geometric mean levels of particulate matter(total dust, respirable dust) for personal sampling were $1.735(SD:0.883)mg/m^3$ for total dust and $0.0711(SD:0.008)mg/m^3$ for respirable dust, respectively. Those for area sampling were $0.625(SD:0.091)mg/m^3$ for total dust and $0.0718(SD:0.012)mg/m^3$ for respirable dust, respectively. The highest geometric averaged concentrations of airborne microorganisms(Total airborne bacteria, total airborne fungi) were detected at $1,181(SD:105)cfu/m^3$ for total airborne bacteria and $683(SD:114)cfu/m^3$ for total airborne fungi, respectively. Concentrations of particulate matters and airborne microorganism in clothes shopping center did not correlate significantly with environmental factors such as temperature or relative humidity. Conclusions: Exposure levelshave not been established for service workers. Thus, health risk assessment for this group is very difficult. Health guidelines for service workers should be established as soon as possible.

• 한국산업보건학회지
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• 제20권1호
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• pp.41-46
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• 2010

The purpose of this study is to assess the level of fungi concentration in the university laboratories in Seoul, Korea, and to investigate factors contributing to these concentrations. The samples were taken from three spots in each laboratory; the top of sink, the center of laboratory, and the front of ventilation system, i.e fume hood at the chemical laboratory and clean bench/biosafety cabinet at the microbial laboratory. Air samples were collected using the single-stage Anderson sampler (Quick Take 30) at a flow rate of 28.3 l/min for 5 min on nutrient media in Petri-dishes located on the impactor. Fifty-two air samples were collected from 19 different laboratories (13 microbiology laboratories, 6 chemistry laboratories) in the university, and concentrations of airborne fungi showed no significant difference (p>0.05) between microbiology and chemistry laboratory, and also no significant difference at three locations (sink, center, front of ventilation system) in microbiology and chemistry laboratories. Average concentrations of fungi in 19 laboratories ranged from 7 to 459 cfu/$m^3$, with an overall Geometric Mean of 52 cfu/$m^3$. Airborne fungi concentrations of 6 samples (12 %) exceeded 150 cfu/$m^3$, the guideline of WHO. The ratios of Indoor/Outdoor for airborne fungi ranged from 0.2 to 4.8 (mean = 1.6). Related factors were measured such as relative humidity, temperature, and laboratory area. Temperature and laboratory area showed no significant relations to concentrations of airborne fungi except for relative humidity in the laboratory Concentrations of fungi were significant different (p<0.01) between rainy or cloudy and sunny. However, there was no significant difference between general ventilation and nongeneral ventilation.

• 한국환경보건학회지
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• 제38권2호
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• pp.118-127
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• 2012

Objectives: This study was undertaken to assess the sanitary conditions in the kitchens of food court/cafeterias and determine seasonal variations. Methods: We measured environmental factors (air temperature, relative humidity, illumination intensity, noise level), and dropping airborne microbes (bacteria and fungi) in the kitchens of eight food court/cafeterias in four seasons (January, April, July, and October). Air temperature and relative humidity were measured with in/out thermo-hygrometers at 1.2-1.5 m above floor level. Illuminance measurement was performed through the multiple point method of Korean Standards (KS). Noise level was measured by the standard methods for the examination of environmental pollution (noise and vibration) of Korea. The estimation of dropping airborne bacteria and fungi was performed through use of Koch's method. Results: The highest kitchen air temperature was in July, and the lowest in January. The average temperature surpassed $21^{\circ}C$ throughout the seasons, suggesting a higher temperature than required for the safe handling of food. Humidity in all the kitchens was measured in the range of 50-60%. Half of the kitchens showed illumination intensities below 300 Lux in April. It was found that the sound pressure level of noise in almost all of the kitchens was higher than 85 dB (A). The highest levels of dropping airborne bacteria and fungi were noted in July. The numbers of airborne bacteria were higher than those of fungi. The levels of dropping airborne bacteria and fungi were affected by air temperature, relative humidity, season, and place. Conclusions: This study indicates that the kitchen environments were unqualified to supply safe food. The hygiene level of the kitchens should be improved.

• 한국환경보건학회지
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• 제38권6호
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• pp.503-509
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• 2012

Objectives: This study was performed in order to evaluate the generation characteristics of airborne bacteria and fungi while operating a household humidifier, in consideration of user habits. Methods: Microbial samples were collected in a closed chamber with a total volume of 2.76 $m^3$, in which a humidifier was operated according to experimental strategies. A cultivation method based on the viable counts of mesophilic heterotrophic bacteria and fungi was performed. Experimental strategies were divided into three classes: the type of water in the water reservoir (tap water, cooled boiled water); the frequency of filling the reservoir (refill every day, no refill); and the sterilization method (sterilization function mode, humidifier disinfectants). Results: Significant increases in the concentration of airborne bacteria were observed while the humidifier was in operation. The concentration had increased to 2,407 $CFU/m^3$ by 120 hours when tap water filled the reservoir without any application of sterilization, while for cooled boiled water, it was merely 393 $CFU/m^3$ at a similar time point. Usages of disinfectant in the water tank were more effective in decreasing bioaerosol generation compared to sterilization function mode operation. Generation characteristics of airborne fungi were similar to those of bacteria, but the levels were not significant in all experiments. Calculated exposure factor can be used as an indicator to compare biorisk exposure. Conclusion: This study identified the potential for bioaerosol generation in indoor environments while operating a household humidifier. User practices were critical in the generation of bioaerosol, or more specifically, airborne bacteria. Proper usage of a humidifier ensures that any biorisks resulting from generated bioaerosol can be prevented.

• 보존과학연구
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• 통권36호
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• pp.64-73
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• 2015

동산문화재 보관처는 사찰과 문중 또는 개인 소유자가 관리하므로 전문적이고 체계적인 보존 관리가 부족하며 이로 인해 열악한 보존환경으로 이어질 수 있다. 실내 공기질에 부유하는 먼지와 미생물은 문화재에 생물학적 훼손을 발생시킨다. 본 연구에서는 다양한 동산문화재들이 보관되어 있는 10곳의 수장시설 또는 전시 및 보관시설에 대하여 실내 공기 중 부유 미생물의 분포 상태를 조사하였다. 그 결과, 수집된 미생물의 집락수는 조사 장소에 따라 큰 차이를 나타냈는데, 가장 미생물 오염이 심각했던 보관처는 전남 영암의 D 유물전시관으로 이곳은 모든 조사 지점에서 $2,000C.F.U/m^3$ 이상이 검출되었으며 대부분의 소장처에서는 $166C.F.U/m^3$ 이상의 진균이 부유하고 있는 것으로 확인되었다. 검출된 미생물을 동정한 결과, Aspergillus sp.와 Penicillium sp., Alternaria sp. Cladosporium sp.등이 공통적으로 확인되었으며 Ceriporia lacerata, Ganoderma carnosum, Myrothecium gramineum 와 Bjerkandera sp. 등과 같은 부후균도 동정되었다. 이러한 진균들은 문화재의 원형 손상 등의 문제를 발생시킬 수 있으므로 부유 진균 농도에 대한 권고기준을 제시하고 소장처의 보존환경 관리 시스템을 마련해야할 것으로 판단된다.

• 한국균학회지
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• 제46권2호
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• pp.122-133
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• 2018

순천 선암사는 산림과 계곡으로 둘러싸여 가파른 지형에 위치하고 있으며 평지보다 온 습도는 높고 바람의 세기가 약하여 생물 피해가 우려되는 곳이다. 이에 계절마다 진균상 조사를 통해 실내 외 공기질에 분포하는 진균의 농도와 다양성을 조사하였다. 외부 공기질에 분포하는 진균의 농도는 봄($276CFU/m^3$)에 가장 높았고, 가을($196CFU/m^3$), 여름($128CFU/m^3$), 겨울($24CFU/m^3$) 순으로 감소하였다. 가장 하단에 위치한 지장전은 여름철에 $337.4CFU/m^3$, 가장 상단에 위치한 원통전은 가을철에는 $333.4CFU/m^3$의 가장 높은 농도를 나타냈다. 실내 외 공기질에서 부유균 농도의 차이가 컸던 계절은 여름철이었으며 $128CFU/m^3$인 외부보다 실내 공기질에 최소 0.5배에서 최대 3배 정도의 많은 진균이 부유하고 있었다. 부유 진균이 가장 많이 수집된 계절은 봄철이었지만 다양성은 여름과 가을철 공기질에서 더 높았는데 여름에는 28속 45종, 가을에는 25속 47종의 진균이 확인되었다. 특히, 가을철에는 모든 조사 지점에서 부유 진균의 분포도가 가장 높았는데 그 중에서 자낭균이 86%를 차지하였고 Cladosporium속이 우점하였다. Penicillim속은16종이 검출되어 종다양성이 가장 높았고 야외보다는 여름과 가을, 겨울철의 실내 공기질에 주로 분포하였다.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2003년도 Challenges and Achievements in Environmental Health
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• pp.181-182
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• 2003

Our study from Feburary to March, 2003, were done in university office A and B, located in Seoul. This study was carried out to investigate the reduction of the airborne fungi before and after we activate the air cleaner which using ionization. And the method of assessment was done by the CAMNEA method. The result was as follows. 1. In the research office A, the concentration of the airborne fungi was 18(${\pm}$11.3) CFU/㎥ before the ionizing air cleaner system was turned on: whereas three days after this result the concentration decreased to less than 1 CFU/㎥. 2. In the laboratory office B, the concentration was 210.6(${\pm}$5.3) CFU/㎥ before using the air cleaner and was decreased to 32.2(t 10.3) CFU/㎥ after using the air cleaner. The remediation rate in the experiment was 85 percentile,

• 한국임상수의학회지
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• 제34권2호
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• pp.76-81
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• 2017

Studies on the concentration of airborne microorganisms in human medicine as a part of a study on the nosocomial infections have been conducted properly, but in veterinary medicine, there has been rarely performed in Korea to the best of study's knowledge. The purpose of this study was to evaluate the distribution of airborne microorganisms and to identify their species in different places in the animal hospital to alert the necessity of thorough cleanliness management. This study evaluated the concentrations of airborne bacteria and fungi in hospital areas, such as patient waiting room, internal medicine ward, surgical ward and radiological diagnostic ward. The concentration of bacteria and fungi was significantly lower (p < 0.05) in two operating rooms and higher in the patient waiting room. The dominant species of bacteria were Micrococcus spp., Staphylococcus spp., and fungi were Penicillium spp., Dermatophyte mold. Animal hospitals need to perform proper procedures for disinfection, sterilization, and environmental cleaning as well as appropriate employee training and monitoring in order to the maximum prevention of the risk of nosocomial and surgical infections.