• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2016.09a
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• pp.156-160
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• 2016

• The Plant Pathology Journal
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• v.35 no.1
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• The Plant Pathology Journal
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• v.37 no.5
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• pp.437-445
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• 2021

Tilletia laevis Kuhn (syn. Tilletia foetida (Wallr.) Liro.) causes wheat common bunt, which is one of the most devastating plant diseases in the world. Common bunt can result in a reduction of 80% or even a total loss of wheat production. In this study, the characteristics of T. laevis infection in compatible wheat plants were defined based on the combination of scanning electron microscopy, transmission electron microscopy and laser scanning confocal microscopy. We found T. laevis could lead to the abnormal growth of wheat tissues and cells, such as leakage of chloroplasts, deformities, disordered arrangements of mesophyll cells and also thickening of the cell wall of mesophyll cells in leaf tissue. What's more, T. laevis teliospores were found in the roots, stems, flag leaves, and glumes of infected wheat plants instead of just in the ovaries, as previously reported. The abnormal characteristics caused by T. laevis may be used for early detection of this pathogen instead of molecular markers in addition to providing theoretical insights into T. laevis and wheat interactions for breeding of common bunt resistance.

• The Plant Pathology Journal
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• v.35 no.3
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• pp.208-218
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• 2019

Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.

• International Journal of Industrial Entomology and Biomaterials
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• v.3 no.1
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• pp.97-99
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• 2001

Seven vegetables were planted in green houses to compare their yields and damages by insect pests based on three treatments, green music (GM), ultra sonic (US) and control. The vegetable yields of GM treatment were increased by an average of 17.81% than that of control for two years. There was a remarkable statistic difference in the yield between GM and respective controls such as radish, young radish, cucumber, spinach and Brassica oleracea var. acephala. There was no notable yield difference between US and controls for the semen vegetables within two years. The population densities of the insect pests in GM and US treatments were lower than controls. Only a few insect pests of some vegetables in GM and US treatments injured more seriously than in control.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.113-113
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• 2002

• Korean journal of applied entomology
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• v.56 no.2
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• pp.203-212
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• 2017

Thirty-three species of insect pests including of two-spotted spider mite, Tetranychus urticae Koch, and air-breathing land snail, Acusta despecta Sowerby, were observed on mulberry for producing mulberry fruits in Jeonbuk province from 2015 to 2016. Of these, 8 species of insect pests, including of mulberry thrips, Pseudodendrothrips mori Niwa, are regarded as major insect pests. Of the 25 species observed before harvesting, 6 species-P. mori, Anomoneura mori, Pseudaulacaspis pentagona, Baris deplanata, Menophra atrilineata and T. urticae-are regarded as major insect pests. In contrast, of the 20 species observed after harvesting, 5 species-P. mori, P. pentagona, Glyphodes pyloalis Walker, Hyphantria cunea Drury and T. urticae-are regarded as major insect pests. Eight of the major insect pest species and nine other insect pest species-including Hexcacentrus unicolor Serville, Malcus japonicus Ishihara et Hasegawa, Halyomorpha halys stal, Xylosandrus germanus Blandford, four species of Repidoptera (Bombyx mandarina Moore, Euproctis subflava Bremer, Acronicta major Bremer, Agrotis trifurca Butler), and Acusta despecta Sowerby-were observed at all sites studied, although occurrence of the insect pests observed on mulberry varied from site to site.

• Journal of Microbiology
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• v.45 no.3
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• pp.219-226
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• 2007

Insertion sequence IS1112 is a repetitive element with a relatively high number of copies in Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight of rice (Oryza sativa L.). Three new loci of IS1112 were identified in seven Chinese strains of Xoo using a single oligonucleotide primer J3; 5'-GCTCA GGTCAGGTGGCCTGG-3' by insertion-sequence-based polymerase chain reaction (IS-PCR). Among the three new loci of IS1112, two were located in the open-reading frame region of genes fhuA and cirA, which encode TonB-dependent receptors, and the third in ISXo2, another type of insertion sequence in Xoo genome. Three variants of IS1112 were identified in those three loci based on their sequence similarities: two were identical to IS1112a and IS1112b, reported in strain PXO86 from the Philippines, while the third was a new member of IS1112, defined as IS1112d. Inserting IS1112 in gene fhuA caused three bases, GGT, to be duplicated at the target site, but inserting it in gene cirA did not cause any duplication in the target site. The diversity of IS1112 sequence and insertion loci in Xoo genome and their potential effects are discussed.

• Korean journal of applied entomology
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• v.17 no.1 s.34
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• pp.1-5
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• 1978

The list of lepidopterous pests on soybean represented in this paper is a result which has been carried out for the survey of insect pests of main crops under the Strengthening Plant Protection Research and Training Project, FAO/UNDP. Forty eight lepidopterous pests which collected on soybean during this period are identified and the general biology of major pests is summarized. Most of materials were collected in the stage of larva, which was injurious stage, on the injurious parts of soybean and reared in the laboratory.

• Molecules and Cells
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• v.28 no.3
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• pp.155-165
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• 2009

The newly sequenced complete mitochondrial genome of the brown marmorated stink bug, Halyomorpha halys($St{\aa}l$) (Hemiptera: Pentatomidae), is a circular molecule of 16,518 bp with a total A+T content of 76.4% and two extensive repeat regions in A+T rich region. Nucleotide composition and codon usage of H. halys are about average when compared with values observed in 19 other published hemipteran mitochondrial genomes. Phylogenetic analyses using these 20 hemipteran mitochondrial genomes support the currently accepted hypothesis that suborders Heteroptera and Auchenorrhyncha form a monophyletic group. The mitochondrial gene arrangements of the 20 genomes are also consistent with our results.