• 한국환경과학회지
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• 제31권11호
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• pp.981-987
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• 2022

This study aimed to investigate the psychological effect of a healing agricultural program using insect resources and to confirm the persistence of the healing effect. The applied insect was silkworm Bombyx mori (Linnaeus) and 28 adolescents from local children's centers were used as the subjects. The healing agricultural program was applied at the centers for 4 weeks, and stress was measured using salivary α-amylase levels and a survey on life satisfaction, happiness, and insect preference. The results indicated that stress levels decreased, and life satisfaction and insect preference increased after participating in the the healing agricultural program. Furthermore, it was confirmed that this healing effect continued until 4 weeks after the end of the program.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.49-55
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• 2022

A total of 20 mulberry varieties preserved in Korea were typed for eight polymorphic microsatellite loci. We obtained 6 to 15 alleles per locus with an average value of 10.6, per-locus observed heterozygosity ranging from 0.35 to 1.00, and per-locus polymorphic information content (PIC) ranging from 0.61 to 0.87, indicating that most loci are highly variable. Phylogenetic analysis using the eight microsatellite loci was sufficiently suitable for classifying 20 mulberry varieties preserved in Korea. A total of 160 variety-specific apomorphic alleles were obtained from eight loci discriminated 20 mulberry varieties. These variety-specific alleles from this analysis are expected to be useful for the discrimination of other mulberry varieties. Furthermore, a substantial number of homozygote loci, represented by 60 among 180 alleles in eight loci were found. These results collectively suggest that these microsatellite locus primers are potentially crucial molecular markers for the eventual classification of mulberry varieties that are preserved as hundreds in Korea.

• 농업과학연구
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• 제45권1호
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• pp.28-37
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• 2018

This study was done to develop environmental risk assessments and a biosafety guide for insect-resistant genetically modified rice at a LMO (Living Modified Organism) isolation field. In the LMO quarantine area of Kyungpook National University, the species diversities and population densities of non-target insects found on insect-resistant genetically modified rice (Bt-9) resistant to Cnaphalocrocis medinalis and on non-GM rices (Dongjin and Ilmi) were investigated. The Bt-9 event was therefore evaluated under field conditions to detect possible impacts on the above ground insects and spiders. The study compared transgenic rice and two non-GM reference rices, Ilmi and Dongjin, at Gunwi in Southern Korea in 2016. Each rice was grown on three $18m^2$ plots with a randomized block design. A total of 4,243 individuals from 43 families and 9 orders were collected from the LMO isolation field. In the three types of rice fields, a total of 1,467 individuals from the insect-resistant genetically modified rice (Bt-9), 1,423 individuals from the Ilmi, and 1,353 individuals from the Dongjin were collected, respectively. There was no difference between the population densities of the non-target insect pests, natural enemies and other insects on the insect-resistant genetically modified rice (Bt-9) and non-GM rices. These results provide the diversity and population density of non-target insects for an environment risk assessment survey on insect-resistant genetically modified rice and could be used as a guideline to make a biosafety assessment method for genetically modified crops.

• 한국농공학회논문집
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• 제61권1호
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• pp.75-83
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• 2019

Fruit bagging is a traditional way to produce high-quality fruit and to prevent damage from insects and diseases. Growing pears by non-bagging is concerned about the damage from insect, it can be controlled by installing a insect net facility. Wind load should be considered to design the insect net facility because it has the risk of collapse due to the strong wind. So we carried out wind tunnel test for measurement of drag force, where the insect net with porosity about 65% is selected as an experimental subject. As a result of the test, drag force was measured to be 244.14 N when insect net area and wind speed are $1m^2$ and 22.7 m/s respectively. And, drag coefficients for the insect net were found to be about 0.55~0.57, which may be used as the preliminary data to design the insect net facilities at the orchard.

• International Journal of Industrial Entomology and Biomaterials
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• 제47권2호
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• pp.126-133
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• 2023

Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the "Daeshim" variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33-43% in a low-temperature forced-air chamber at -5 ℃, then slowly frozen from -5 ℃ to -20 ℃, and preserved in liquid nitrogen (-196 ℃). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the "Daeshim" variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen.

• 한국응용곤충학회지
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• 제48권4호
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• pp.453-457
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• 2009

본 연구는 2005년부터 2007년까지 수원, 영양, 부여와 예천의 곤충 생태원 부지에서 곤충강의 다양성을 조사하였다. 월별 곤충 개체군 크기는 6월부터 8월에 네 조사구역 모두에서 가장 컸다. 전체적으로 딱정벌레목의 곤충이 가장 많이 채집되었으며, 노린재목과 메뚜기목의 곤충이 뒤를 이었다. 각 곤충생태원은 지리적 특색에 맞는 고유한 교육프로그램을 운영하고 있었다. 이러한 연구와 생태원의 활동은 그 지역의 곤충과 같은 생물의 다양성을 보존하고 관람객에게 인식시키는데 중요한 역할을 할 것이다.

• International Journal of Industrial Entomology and Biomaterials
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• 제31권1호
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• pp.7-13
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• 2015

In an effort to obtain foundational data required to evaluate the therapeutic effect of educational insect rearing, this study surveyed current educational pet insect rearing practices in Korea and examined the expected psychological effects from participating in the activity. Data were collected from those who visited the special insect exhibition in May 2013 using a self-reporting survey. The results indicated that 48.3% of the responders had experienced insect rearing, and that rhino and stag beetles were the most commonly reared insects. Most of the respondents (83.1%) reported they were not currently rearing any insects, which suggested that insect rearing tends to be a one-time experience. Expected psychological effects could be divided into emotional (56.3%) and cognitive (41.8%) effects, and these varied by age and gender. The differences in expected psychological effects were particularly clear between adults and adolescents, the latter of whom were the main participants in the activity. It is therefore necessary to develop standardized manuals and care programs for more diverse insect species with which to help keep up consumer interest in educational insect rearing. These efforts will help champion the psychotherapeutic effects of educational pet insect rearing and enhance its role as a learning aid.

• 농촌지도와개발
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• 제26권3호
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• pp.153-163
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• 2019

The study aims to examine the psychological effects of insect-based experience activities. A sample of 167 elementary students was hired for the study. Students in the experimental group participated in insect-based experience activities using swallowtail butterfly. Collected data are analyzed with analysis of variance and paired-sample T-test in SPSS 25.0. The results of this study are as follows 1) Insect based experience activities show positive effects in psychological and physiological aspects, 2) and students and teacher showed high satisfaction in insect-based program. In conclusion, this study contributes to providing fundamental information for the development of agro-healing programs using insect and design guidelines for identifying the characteristics of each healing program. Further research should be extended to different types of student and the development of diverse insect-based activities.

• International Journal of Industrial Entomology and Biomaterials
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• 제29권2호
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• pp.214-219
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• 2014

We prepared antibodies from insect glycosaminoglycans (GAGs) and assayed the titer. Nine polyclonal antibodies against insect GAGs were raised for development of an ELISA in biological fluids (mice serum). The 3th booster collection of antiserum of BALB/c mice as a primarily antibody was assayed for titer determination by ELISA method. In sandwich ELISA of GAGs derived from Isaria sinclairii or other insects, antiserum from insect GAGs gave satisfactory results for so potent antibody(100: 1~1000:1) raising (manufacturing) agent in range of 10 ng/ml.

• 한국응용곤충학회지
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• 제59권3호
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• pp.169-175
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• 2020

본 연구를 통해 애완곤충산업에 이용될 수 있는 곤충 종에 대한 평가지표를 개발하였다. 분석 방법으로는 전문가 의견조사인 델파이(Delphi) 방법을 이용하여 4개의 대분류와 9개의 중분류로 구성된 평가항목을 도출하였다. 또한, 계층분석과정(AHP)을 활용하여 애완곤충 종을 선발하기 위한 평가기준을 마련하였고, 적용 시험을 통해 점수의 체계화를 구축하였다.