• Title/Summary/Keyword: after school life

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The Viability Change of Yeast Cell Responding to Trehalose Accumulation and Maintaining Neutral Trehalase Activity under Extracellular pH Acidified by $H_2SO_4$

  • Jin, Ingnyol;Yun, Haesun;Paik, Sanhkyoo;Kim, Ilsup;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.12 no.2
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    • pp.47-52
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    • 2002
  • Saccharomyces cerevisiae KNU5377 (KNU5377) and S. cerevisiae ATCC24858 (ATCC24858) were exposed to $H_2SO_4$ as a stress, which was added at various concentrations to a YPD media. The growth of KNU5377 was reduced to approximately 60% in the YPD media containing 40 nm sulfuric acid when compared to the non-stressed condition. When their growth was monitored during an overnight culture, two strains, KNU5377 and ATCC24858, could not grow when exposed to over 50 mM of sulfuric acid. After a short exposure to this acid for 1 h, KNU5377 exhibited stronger resistance against $H_2SO_4$ than ATCC24858. The neutral trehalase activity of KNU5377 unchanged despite under various concentrations of $H_2SO_4$. In contrast, It at of ATCC24858 was much low at higher $H_2SO_4$concentrations. Trehalose, a non-reducing disaccharide, was maximally accumulated after a short exposure to 60 nm $H_2SO_4$ for KNU5377, but it was reduced under more severe stressful conditions. These results suggest that KNU5377 should modulate the trehalose concentrations under the severe stress condition of high sulfuric acid concentrations. The most highly induced protein in the KNU5377 exposed to sulfuric acid was found to be an approximately 23 kDa protein, which was revealed to be the 605 large subunit ribosomal protein, Ll3 by FASTA search results.

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Hyperglycemia aggravates decrease in alpha-synuclein expression in a middle cerebral artery occlusion model

  • Kang, Ju-Bin;Kim, Dong-Kyun;Park, Dong-Ju;Shah, Murad-Ali;Kim, Myeong-Ok;Jung, Eun-Jung;Lee, Han-Shin;Koh, Phil-Ok
    • Laboraroty Animal Research
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    • v.34 no.4
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    • pp.195-202
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    • 2018
  • Hyperglycemia is one of the major risk factors for stroke. Hyperglycemia can lead to a more extensive infarct volume, aggravate neuronal damage after cerebral ischemia. ${\alpha}$-Synuclein is especially abundant in neuronal tissue, where it underlies the etiopathology of several neurodegenerative diseases. This study investigated whether hyperglycemic conditions regulate the expression of ${\alpha}$-synuclein in middle cerebral artery occlusion (MCAO)-induced cerebral ischemic injury. Male Sprague-Dawley rats were treated with streptozotocin (40 mg/kg) via intraperitoneal injection to induce hyperglycemic conditions. MCAO were performed four weeks after streptozotocin injection to induce focal cerebral ischemia, and cerebral cortex tissues were obtained 24 hours after MCAO. We confirmed that MCAO induced neurological functional deficits and cerebral infarction, and these changes were more extensive in diabetic animals compared to non-diabetic animals. Moreover, we identified a decrease in ${\alpha}$-synuclein after MCAO injury. Diabetic animals showed a more serious decrease in ${\alpha}$-synuclein than non-diabetic animals. Western blot and reverse-transcription PCR analyses confirmed more extensive decreases in ${\alpha}$-synuclein expression in MCAO-injured animals with diabetic condition than these of non-diabetic animals. It is accepted that ${\alpha}$-synuclein modulates neuronal cell death and exerts a neuroprotective effect. Thus, the results of this study suggest that hyperglycemic conditions cause more serious brain damage in ischemic brain injuries by decreasing ${\alpha}$-synuclein expression.

Expression and evaluation of porcine circovirus type 2 capsid protein mediated by recombinant adeno-associated virus 8

  • Li, Shuang;Wang, Bo;Jiang, Shun;Lan, Xiaohui;Qiao, Yongbo;Nie, Jiaojiao;Yin, Yuhe;Shi, Yuhua;Kong, Wei;Shan, Yaming
    • Journal of Veterinary Science
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    • v.22 no.1
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    • pp.8.1-8.11
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    • 2021
  • Background: Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide. Objectives: A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy. Methods: In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vivo after gene transfer. Results: The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2. Conclusions: The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.

Uptake and Translocation of Ethoprophos Mixed with Soil for Cultivation of Preceding Crop into Succeeding Crop (전작물 재배를 위해 토양에 혼화처리된 Ethoprophos의 후작물 흡수이행)

  • Kwak, Se-Yeon;Lee, Sang-Hyeob;Kim, Hyo-Young;Shin, Byung-Gon;Kim, Jang-Eok
    • Korean Journal of Environmental Agriculture
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    • v.40 no.2
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    • pp.92-98
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    • 2021
  • BACKGROUND: Unintentional residual pesticide in soil derived from preceding crops and the transfer to succeeding crops was considered a critical barrier for positive list system (PLS). Thus, an uncertain risk is predicted for ethoprophos applied at cultivation of preceding crop (Korean cabbage) to succeeding crop (spinach). METHODS AND RESULTS: Ethoprophos was treated on soil following the recommended dose and 5 times dose according to the safe use guidelines for Korean cabbage after seeding. On the 4 days after harvesting of preceding crop, spinach was sowed. The initial residual amounts of ethoprophos on soil (7.081-19.493 mg/kg) were decreased to 3.832-7.218 mg/kg until the harvest of Korean cabbage, and then finally decreased to 0.011-0.079 mg/kg after spinach cultivation. The uptake rates of ethoprophos from soil by Korean cabbage were 0.01-0.03% and distributed to root (0.150-0.903 mg/kg) and shoot (0.021-0.151 mg/kg), respectively. The residual amounts of uptake and translocation from preceding crop cultivated soil to spinach edible part were found to be below LOQ. CONCLUSION: The plant back internal (PBI) for ethoprophos is not recommended during sequential cultivation of leafy vegetables, since the residual amounts of ethoprophos in spinach were less than MRL (0.02 mg/kg).

The Effect of Iron-enriched Cereal Breakfast and Nutrition Education on the Nutritional Status and Life Style of Elementary School Students (서울지역 아동의 철분강화 시리얼 보충과 영양교육이 영양상태 및 생활습관에 미치는 영향)

  • You, So-Hyoung;You, Hyun-Joo;Park, Yong-Soon
    • Journal of Nutrition and Health
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    • v.40 no.8
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    • pp.728-735
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    • 2007
  • The present study was conducted to investigate the effect of iron-enriched cereal supplement and nutrition education program on nutritional status and life style of children. Elementary school students(n=170) aged of 11 years old at Seoul were participated. Subjects were supplemented with iron-enriched cereal for breakfast and provided with 2 sessions of nutrition education during 3 months. Intake of carbohydrate, iron, sodium, zinc, vitamin A, vitamin $B_1$, vitamin $B_2$, vitamin C, niacin, and folic acid was significantly increased after cereal supplement. Caloric intake was similar to KDRIs during both before and after cereal supplement. There was no improvement on exercise and dietary habits after nutrition education. Male students tended to sleep later, wake up early, and ate snack less than female. Cereal supplement was effective on improving nutritional status, but short-term nutrition education had no effect on modification of life style. Thus, further studies are required to develop long-term education program effective on improving lift style and eating habits in children.

Development and Effectiveness Verification of a Group Art Therapy Program for Improving School Life Adaptation of Children in Multicultural Families - Focusing on Analysis by Kinetic School Drawing(KSD) - (다문화가정 자녀의 학교생활적응 향상을 위한 집단미술치료 프로그램의 개발과 효과 검증 - 동적 학교생활화(KSD)에 의한 분석을 중심으로-)

  • Park, Soo-Jung;Choi, Youn-Shil
    • Journal of Families and Better Life
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    • v.28 no.6
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    • pp.1-18
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    • 2010
  • The purpose of this study is to develop and verify the effectiveness of a group art therapy program to help children from multicultural families adapt to school life by using Kinetic School Drawing(KSD). The result of this study showed that the group art therapy program of the multicultural family's children may have a positive effect in aiding their school life adaptation. The results showed considerable and statistical outcome in the fore and after drawing inspection of the dynamic KSD. Also, the size of the self-image became bigger and their expressions has been increased. The qualitative analysis also showed a huge change in dynamistic personal relationships due to the relationship with their peers with the same aged and the tutor. This study emphasizes the importance of the group art therapy program to be applied before children are admitted to school because of growing recognition of concern about the maladjustment of rapidly growing multicultural family's children. It is also suggests that the group art therapy program of growing multicultural family's children should be viewed with an ecological point of view.

A Study on the Sanitary Knowledge and Performance Levels of School Foodservice Employees in Gyeonggi-Do by Sanitary Education (경기도 학교 급식 조리 종사자의 위생 교육을 통한 위생 지식 및 수행 수준 연구)

  • Oh, You-Ri;Gil, Bog-Im
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.5
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    • pp.738-745
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    • 2007
  • The purpose of this study was to investigate the effects of sanitary education on the sanitary knowledge and performance levels of school foodservice employees in Gyeonggi-do, particularly in Siheung, Ansan, Gunpo and Anyang City. Four times we provided questionnaires consisting of the same content to each individual. The first and second questionnaires were answered before and after the first sanitary education, respectively, and the third and forth questionnaires were answered before and after the second sanitary education, respectively. The changes in sanitary knowledge and performance level before and after education were statistically significantly different(p<0.01). Most of the school foodservice operations performed sanitary education once a month, and recognized the need for sanitary education as well as experienced improvements through sanitary education. Moreover, the fact that sanitary knowledge following the second education was higher than after the first education suggests the possibility of improving sanitary practices of foodservice employees through continual education.

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Comparison of Microtubule Distributions between Somatic Cell Nuclear Transfer and Parthenogenetic Porcine Embryos

  • Park, Joo-Hee;Kwon, Dae-JinK;Lee, Beom-Ki;Hwang, In-Sun;Park, Choon-Keun;Yang, Boo-Keun;Cheong, Hee-Tae
    • Reproductive and Developmental Biology
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    • v.33 no.1
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    • pp.13-18
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    • 2009
  • The aim of this study was to examine the microtubule distributions of somatic cell nuclear transfer (SCNT) and parthenogenetic porcine embryos. Porcine SCNT embryos were produced by fusion of serum-starved fetal fibroblast cells with enucleated oocytes. Reconstituted and mature oocytes were activated by electric pulses combined with 6-dimethlyaminopurine treatment. SCNT and parthenogenetic embryos were cultured in vitro for 6 days. Microtubule assembly of embryos was examined by confocal microscopy 1 hr and 20 hr after fusion or activation, respectively. The proportions of embryos developed to the blastocyst stage were 25.7% and 30.4% in SCNT and parthenogenetic embryos, respectively. The frequency of embryos showing $\beta$-tubulins was 81.8% in parthenogenetic embryos, whereas 31.3% in SCNT embryos 1 hr after activation or fusion. The frequency of the embryos underwent normal mitotic phase was low in SCNT embryos (40.6%) compared to that of parthenogenetic ones (59.7%) 20 hr after fusion or activation (p<0.05). The rate of SCNT embryos with an abnormal mitosis pattern is about twice compared to that of parthenogenetic ones. The spindle assembly and its distribution of SCNT embryos in the first mitotic phase were not different from those of parthenogenetic ones. The result shows that although microtubule distribution of porcine SCNT embryos shortly after fusion is different from parthenogenetic embryos, and the frequency of abnormal mitosis 20 hr after fusion or activation is slightly increased in SCNT embryos, microtubule distributions at the first mitotic phase are similar in both SCNT and parthenogenetic embryos.

The Effects of Dream Start Participation on the Use of After-School Programs : Focusing on the Diversity and Amount of the Programs in Use (드림스타트 참여가 방과후 서비스 이용에 미치는 영향 : 방과후 서비스의 종류와 이용량을 중심으로)

  • Jung, Sun Young;Song, Mi Ryung;Cho, Yu Ri
    • Journal of Families and Better Life
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    • v.33 no.3
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    • pp.1-14
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    • 2015
  • This study examined the effects of Dream Start participation on the use of after-school programs, considering its emphasis on the utilization of community resources. For this, it compared the use of after-school programs in terms of the number of types and the total amount of time between children who had been participating in Dream Start and those who had not. The main findings are as follows. First, when all other conditions were held constant, children in Dream Start used more types of after-school programs compared to their correspondents. Second, children in Dream Start were more likely to use two types of after-school programs over weekdays or three types of programs for several days a week compared to their correspondents. Third, children in Dream Start spent less amount of time in the after-school programs compared to their correspondents. Even though this study found the effectiveness of Dream Start as a facilitator of community resource utilization, future research needs to examine the effects of after-school program use in terms of its diversity and amount on the development of low-income children.

Comparison of the Efficiency of Propylene Glycol and Distilled Water as Vehicles for Intracanal Medicaments.

  • Kota, K.;Cruz, E.V.;Iwaku, M.;Hoshino, E.
    • Proceedings of the KACD Conference
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    • 2001.11a
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    • pp.565.1-565
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    • 2001
  • This study aimed to compare the efficiency of propylene glycol (PG) and distilled water (DW) as vehicles that would allow diffusion of dye through the root canal system. Human maxillary central incisors were chosen and de-crowned. After enlarging the upper part of the root canal with a Peeso reamer, cementum covering the upper third of the root was removed. The roots were ultrasonically irrigated with 5% NaOCI to remove smear layer.(omitted)

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