• 한국의류학회지
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• 제20권6호
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• pp.992-1001
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• 1996

The binding of polyethylene glycol (PEG, average molecular weight 600) to cotton fabrics was achieved by using pad-dry-cure process in the presence of citric acid, MgCl3·6H3O, DMDHEU, and TEAHCL. Treated fabrics were dyed with direct, acid, and basic dye. Wrinkle recovery angles, durable press rate, wettability, dyeability and color fastness to washing of all treated cottons were evaluated. The results of this study were as follows: 1. The wrinkle resistance of the PEG treated cottons was increased by increasing PEG and DMDHEU concentration. 2. The wettability of the PEG treated cottons was decreased by increasing PEG and DMDHEU concentration, increased by increasing TEAHCL concentration. 3. PEG/DMDHEU/TEAHCL treated cottons had greater affinity on direct, acid, and basic dye than untreated cottons, and dyeability of the modified cottons was improved compare to untreated fabrics. 4. Color fastness to washing of the PEG/DMDHEU/TEAHCL treated cottons was good except for the wash fastness of the direct dye.

• BMB Reports
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• 제43권5호
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• pp.319-324
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• 2010

Experimental bioinformatics data obtained from an E. coli cell-based eukaryotic protein purification experiment were analyzed in order to identify any bottleneck as well as the factors affecting the target purification. All targets were expressed as His-tagged maltose-binding protein (MBP) fusion constructs and were initially purified by immobilized metal affinity chromatography (IMAC). The targets were subsequently separated from the His-tagged MBP through TEV protease cleavage followed by a second IMAC isolation. Of the 743 total purification trials, 342 yielded more than 3 mg of target proteins for structural studies. The major reason for failure of target purification was poor TEV proteolysis. The overall success rate for target purification decreased linearly as cysteine content or isoelectric point (pI) of the target increased. This pattern of pI versus overall success rate strongly suggests that pI should be incorporated into target scoring criteria with a threshold value.

• 생명과학회지
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• 제12권4호
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• pp.460-463
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• 2002

Thyroglobulin과 반응하는 monoclonal antibody를 3클론(TN-1, TN-2 및 TN-3)을 확립하였다. 이들 중 1클론 (TN-2)은 thyroglobulin의 3차원적인 구조를 인식하면서 음전하를 띠는 자가항원의 일종인 phosphatidylserine과도 교차반응성을 나타내었다. 따라서 TN-2를 이용한 자가면역성 갑상선염의 진단 및 치료에의 적용효과가 기대된다.

• 한국환경과학회지
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• 제6권2호
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• pp.189-194
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• 1997

Hornet venom의 독성 peptide인 mastoparan과 mastoparan-B를 solid phase peptide syn- thesis method로 합성한 후 이들과 phospholipid와의 상호작용, 이들의 항균 활성 및 용혈 활성 을 조사하였다. 두가지 독성 peptide 모두 중성 liposome에서 저 농도에서도 dye release를 유도할 수 있었다. 중성 liposome에 대한 mastoparan-B의 결합 친화도는 산성 liposome에서 보다 작았다. Mastoparan과 mastoparan-B는 두가지 모두 그람 양성 세균에 대하여 강한 항균 활성을 가지고 있었으나 그람 음성 세균에 대해서는 각각 약하거나 유력한 활성을 보였다. Mastoparan과 mastoparan-B는 5$\mu$M의 낮은 농도에서도 적혈구를 분해시키는 독성을 보였다.

• 약학회지
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• 제31권2호
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• pp.64-69
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• 1987

Monoclonal antibodies against human chorionic gonadotropin (hCG) were prepared and characterized by examining isotype, epitope binding, cross reactivity and affinity constants. And a sandwich type enzyme immunoassay for native hCG was developed with solid phase monoclonal antibody against the conformational determinant expressed only on native hCG and horseradish peroxidase conjugated monoclonal antibody against the $\beta$-subunit of hCG. The assay was sensitive to 1 mIU hCG/ml and shown a linear response up to 200 mIU hCG/ml. The cross reactivity for luteinizing hormone and $\beta$-subunit of hCG were 1% and 0.18%, respectively.

• 환경생물
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• 제22권4호
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• pp.479-486
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• 2004

Phytoestrogens display estrogen-like activity because of their structural similarity to human estrogens and exhibit high affinity binding for the estrogen receptors (ERs). The prevalence of phytoestrogens in our diets and the biological effects that they may cause need to be fully examined. ER is the ancestral receptor from which all other steroid receptors have evolved. Although phytoestrogens serve specific signaling functions between the plants and insects, fungi, and bacteria, many chemical signals are often misinterpreted as estrogenic signals in non-target organisms such as vertebrates. There are no ERs in plants or in their most common partners, insects. However, Rhizobium soil bacteria have NodD proteins which is an intended target of phytoestrogen signaling and share genetic homology with the ER. These two evolutionarily distant receptors both recognize and respond to a shared group of chemical signals and ligands, including both agonists and antagonists. This review briefly summarizes estrogen and estrogen receptors, kinds of important phytoestrogens, their health effects as well as some of the evolutionary aspects of mechanism by which phytoestrogen mimics the endogenous ER signaling in our body.

• 한국환경과학회지
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• 제7권1호
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• pp.62-66
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• 1998

Toxic Mastoparan B(MP-B) which is purified from the venom of the hornet Vespa basalis is a cationic amphlphilic tetradecapeptide. MP-B and Its Ala-substituted analogues were synthesized by solld phase method and the toxic peptide-membrane interactions were examined by circular dichroism(CD) spectra, fluorescence spectra, and leakage abilities in phospholipid membranes. In the presence of phospholipid vesicles, synthetic MP-B and its analogues formed amphiphilic -helical structures, but in the buffer soletion, those exhibited random coil conformation as measured by CD. Fluorescence spectra of MP-B and its analogues which indicated the binding affinity of peptide on phospholipid vesicles showed that the replacement of Lys at position 2 and 11 with Ala caused a remarkable effect in the blue shalt and that at position 2, in the leakage ability of the peptide.

• Biomolecules & Therapeutics
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• 제22권2호
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• pp.93-99
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• 2014

The interaction between viral HA (hemagglutinin) and oligosaccharide of the host plays an important role in the infection and transmission of avian and human flu viruses. Until now, this interaction has been classified by sialyl(${\alpha}2-3$) or sialyl(${\alpha}2-6$) linkage specificity of oligosaccharide moieties for avian or human virus, respectively. In the case of H5N1 and newly mutated flu viruses, classification based on the linkage type does not correlate with human infection and human-to-human transmission of these viruses. It is newly suggested that flu infection and transmission to humans require high affinity binding to the extended conformation with long length sialyl(${\alpha}2-6$)galactose containing oligosaccharides. On the other hand, the avian flu virus requires folded conformation with sialyl(${\alpha}2-3$) or short length sialyl(${\alpha}2-6$) containing trisaccharides. This suggests a potential future direction for the development of new species-specific antiviral drugs to prevent and treat pandemic flu.

• 한국수정란이식학회지
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• 제10권1호
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• pp.15-22
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• 1995

Since the turn of the century, scientists have earnestly sought to develop a single laboratory assay or combination of laboratory assays which accurately predict the fertility of a semen sample. Most of these assays have focused on evaluating physical characteristics of sperm such as motility, viability, acrosomal integrity and morphology. In recent years new approches have been used to assess the functional aspects of a sperm that are needed to reach the oocyte, fertilize it and contribute to successful embryo development. Among these techniques are the ability of sperm to undergo a heparin induced acrosome reaction and in vitro fertilization, and the affinity of sperm to bind heparin binding protein. Intensification of research efforts in the area of control of sperm fertilizing ability should be a high priority, in view of undoubted benifits both to our basic understanding of sperm fertilizing ability and to our ability to modify it for Al industry.

• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1316-1319
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• 2006

An antibody displayed phage collection (SBAE-2R), screened from a human synthetic phage antibody library (Fab 21ox), was used for the determination of dextran. The dextran-binding affinity was determined by serologically specific transmission electron microscopy (TEM) and a paper dipstick assay. The phage collection was distributed over the dextrancoated grids with 39$\pm$25 phages/$\mu$m$^2$ on the grids. Phages were not seen on dextran-coated grids exposed to the Fab 2lox phage library. The phage collection (SBAE-2R) produced 54$\pm$3 color normalized intensity (N.I.) from 125 ppm to 1,000 ppm of dextran and 5$\pm$1 (N.I.) for 63 ppm of dextran in a paper dipstick assay. This research extends the analytical options for dextran analysis by antibody displayed phage with a minimum of equipment usage.