• 한국자원식물학회지
• /
• 제30권6호
• /
• pp.699-706
• /
• 2017

For rapid production of freesia 'Shiny Gold' shoots by using a bioreactor, several culture conditions were investigated. Young shoots (< 1 cm) obtained from freesia corm section in vitro were used as plant materials for this experiment. As a basic experimental environment, 20 young shoots were inoculated into a 5 L balloon type bubble reactor which contained 1 L 1/2 strength MS medium supplemented with 30 g sucrose (3%), and the aeration was 0.1 vvm (vessel volumes per minute). The bioreactors were placed in a growth room with $23^{\circ}C$ temperature, 60% relative humidity and $60{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ light condition (16 h/8 h, day/night). The concentrations of MS media were set with 1/4, 1/2, 1 strength, medium volume 10, 20, 40%, sucrose concentration 3, 6, 9%, and aeration 0.1, 0.2, 0.4 vvm. After 4 weeks of cultivation, the growth indexes including the fresh and dry weight, and plant height were evaluated. At the same time, the consumption, pH, and EC of medium were estimated 4 weeks after incubating. The best results were achieved when 40 young shoots were incubated in a bioreactor in which 1 L of 1/2 strength MS medium supplemented with 6% sucrose was used for the rapid production of freesia shoots. The shoots were 17 cm in plant height and 1.0 g in fresh weight only 4 weeks after incubation which could be a good plant material suitable for corm enlargement in vitro. No correlation was observed between the growth of freesia shoots and the consumption, pH or EC of medium.

• 식물병연구
• /
• 제12권3호
• /
• pp.164-167
• /
• 2006

배 생육기 발생되는 과피얼룩과의 발생 요인 및 방지 기술을 확립하기 위해 3년 간 시험한 결과를 요약하면 다음과 같다. 배 과피에 발생되는 과피얼룩 발생시기는 유과기부터 수확기에 발생하며, 발생 초기 부정형의 검은색 반점이 생기고 발생 후 시간이 경과되면서 갈색으로 되었다. 봉지 종류에 따른 과피얼룩과 발생 정도는 광 투과량이 적고 속 봉지의 투기성 및 투수성이 불량할수록 발생이 많았으며, 과실 부위별 발생 정도는 과실 적도를 중심으로 상부보다 하부쪽 발생이 현저히 많았다. 강우가 많거나 재배적으로 수관 내 가지 밀도가 많아 채광, 통풍이 불량한 과원에서 발생이 많았다. 약제방제는 수관 약제 살포보다 동계 약제인 석회유황합제 살포+잡초제거에 의해 과수원 내 병원균의 밀도를 감소시키는 것이 효과적이었다.

• 한국미생물·생명공학회지
• /
• 제6권3호
• /
• pp.121-127
• /
• 1978

PDase의 생산하는 데에 탄소원으로 sucrose 3% 금속이온으로는 $Ca^{2+}$, Co$^{2+}$, $Mn^{2+}$ 이온등이 좋은 효과를 나타내 주었고 통기는 많을 수록 좋았으며 초기 pH 8의 배지에서 48시간 배양할 때 가장 좋은 결과를 나타내 주었다. 이렇게 하여 생산된 PDase는 pH 8 부근에서 작용온도 6$0^{\circ}C$일 때 최대효소 활성을 나타냈다.

• 한국토양환경학회지
• /
• 제3권2호
• /
• pp.79-88
• /
• 1998

본 연구는 공기를 주입하여 매립지가스를 치환하고자 할 때 공기공급방법이 매립지가스제거에 미치는 영향을 검토하고자 실시하였다. 공기공급방법으로는 공기를 직접 불어넣는 방법과 매립지가스를 추출함으로써 대기중의 공기가 내부로 유입되도록 하는 방법을 사용하였다. 또한 한 주입정에서 공기를 주입하고 동시에 다른 추출정에서 가스를 추출하는 공정도 실시하여 운전모드에 따른 매립지가스 제거 효율을 비교검토하였다. 공기주입 및 가스추출시 유량은 15$\textrm{m}^3$/min으로 동일하였다. 추출모드나 주입/추출모드로 운전하는 것보다 공기주입모드로 운전하는 것이 매립지가스 제거에 가장 효과적이었으며 추출모드는 매립지가스 제거에 가장 적합하지 못하였다. 공기주입과 추출을 동시에 실시하는 경우에도 주입모드에 비하여 매립지가스 제거효율을 크게 향상시키지는 못하였다.

• Journal of Preventive Medicine and Public Health
• /
• 제25권1호
• /
• pp.34-43
• /
• 1992

To investigate the effect of hyperoxia on EKG findings and to evaluate the applicability of EKG as noninvasive monitoring index of oxygen toxicity, 38 rabbits were continuously exposed to 6 different conditions-3 hyperbaric oxygenations (HBO-2.5, 3.5 and 5ATA, 100% $O_2$), normobaric oxygenation (NBO,100% $O_2$), hyperbaric aeration (HBA-5ATA, 21% $O_2$) and normobaric aeration (NBA, 21% $O_2$)-for 120 minutes and their EKG and time to dyspnea and convulsion were recorded. Dyspnea and death were observed in exposure conditions of HBO-3.5 and HBO-5 (Positive rate of dyspnea 10%, 100%, death : 10%, 25%, respectively) only, and convulsion in 4 oxygenation groups (NBO;20%, HBO-2.5;20%, HBO-3.5;20%, HBO-5;88%). Abnormal EKG findings included arrhythmia and ST-T changes and the incidences was increasing with doses(partial pressure of oxygen). In addition to EKG change, findings observed during exposure were dyspnea and convulsion in the order of appearance and when non specific ST-T change was accepted as positive(abnormal) finding, the frequency of abnormal EKG was statistically significant(p<0.01), but when it was excluded from positive results, the frequency of EKG change was not significant(p>0.05). These results suggest that the effect of hyperoxia on heart is myocardial ischemia and arrhythmia, that oxygenation more than 3.5ATA causes myocardial damage in 120 minutes exposure, and that EKG is valuable as monitoring index of oxygen toxicity.

• 한국물환경학회지
• /
• 제37권6호
• /
• pp.433-441
• /
• 2021

The aim of this study was to optimize the ozonation and ceramic membrane integrated process for greywater reclamation. The integrated process is a repeated sequential process of filtration and backwash with the same ceramic membrane. Also, this study used ozone and oxygen gas for the backwashing process to compare backwashing efficiency. The study results revealed that the optimum filtration and backwash time for the process was 10 minutes each when comparing the filtrate flow and membrane recovery rate. The integrated process was operated at three different operating conditions with i) 10 minutes for filtration and 10 minutes for ozonation, ii) 10 minutes for filtration and 10 minute for oxygen aeration, and iii) continuous filtration without any aeration for synthetic greywater. The integrated process with ozone backwashing could produce 0.55 L/min of filtrate with an average of 18.42% permeability recovery, while the oxygen backwashing produced 0.47 L/min and 6.26%, respectively. And without any backwashing, the integrated process could produce 0.29 L/min. This shows that the ozone backwash process is capable of periodically recovering from membrane fouling. The resistance of the fouled membrane was approximately 34.4% for the process with ozone backwashing, whereas the resistance was restored by 10.8% for the process with oxygen backwashing. Despite the periodical ozone backwashing and chemical cleaning, irreversible fouling gradually increased approximately 3 to 4%. Approximately 97.6% and 15% turbidity and TOC were removed by ceramic membrane filtration, respectively. Therefore, the integrated process with ozonation and ceramic membrane filtration is a potential greywater treatment process.

• 한국미생물·생명공학회지
• /
• 제25권4호
• /
• pp.427-433
• /
• 1997

Sludge Returned CMAS process was applied to treat the wastewater from electric accessory manufacturing company while this type of wastewater was usually treated by chemical process. This result show that the removal rate of TCOD was about 70-80% regardless of hydraulic retention time, On the contrary, the removal rate of BOD was abtained in a range of 77-92% depending on hydraulic retention time. In order to remove more than 80% of organic materials with the proposed process, the F/M ratio should be maintained below 0.17. In this case, the calculated value of organic removal rate, Km, was calculated to be 1.26 hr$^{-1}$, and the ratio of cell synthesis/total energy was 0.32 and 0.26 for COD and BOD base, respectively. The yield coefficient was calculated to be 0.242 and the half velocity coefficient was 0.3 hr$^{-1}$. The value of endogenous respiration coefficient was 0.02 hr$^{-1}$. The measured effluent BOD concentration, MLSS concentration in aeration tank, oxygen uptake rate, and sludge production were matched relatively well with the calculated values using above coefficients, In order to optimize the dewatering of sludge, the hydraulic retention time was recommended to be 15. 6 hrs. These results indicate that the wastewater from an eletric accessory manufacturing company can be treated safely with a biological process.

• 한국토양비료학회지
• /
• 제42권3호
• /
• pp.145-151
• /
• 2009

논에서 전환된 포도원(전환포도원)의 전환 후 년수 및 토양특성별 물리적 특성변화 양상을 구명하여 전환지 생산성 향상을 위한 합리적 토양 관리대책을 제시코자 전환지가 많은 경북 김천, 영천지역을 중심으로 포도원과 인근 벼 재배지 50개 지점을 조사 분석한 결과 전환포도원의 이랑 높이는 토성이 세립질이고 배수가 불량할수록 높았고, 토양단면 내 반문의 출현깊이(Ap층 두께)는 조립질 토양에서 깊은 경향이었음. 전환지는 인근 유사토양 벼 재배지 작토층 토색의 경우 색상이 밝아지는 경향을 보여 회색도가 벼 재배지의 경우 20인데 비해 전환지는 5로 감소했고, 이 경향은 토양 배수조건이 양호한 토양에서 현저하였음. 전환여부에 따른 투기도 차이는 토심10cm 이하에서 현저했고 토심 30cm 이하에서는 뚜렷한 차이가 없었고 전환지에서 투기도에 미치는 토양특성의 영향은 세립질의 경우 이랑의 높이가, 조립질은 배수등급이었다. 전환포도원의 물리성은 전환 후 년수가 경과함에 따라 공극율과 내수성입단이 증가하는 경향이었고, 원추관입저항의 경우 휴립한 부위는 $5kg/cm^2$ 이하인 반면, 그 이하의 부위는 $10kg/cm^2$ 이상을 보였음. 이상의 결과로 볼 때 전환포도원의 토양특성은 세립질보다 조립질이 쉽게 변하는 경향이었고, 배수불량인 세립질토양에서는 고휴재배 등 적절한 표토관리가 요구되었다.

• 한국농공학회지
• /
• 제35권2호
• /
• pp.92-103
• /
• 1993

A sunday system with a horizontal bin-type composter was constructed and operated to evaluate its composting performance for four days for each test in October, 1992. A sundry system is one of popular systems for composting livestock manure, of which main benefit is to utilize unlimited, clean, and free solar radiation. A rectangular concrete bin(composter) with dimension of 300cm(length) X90cm(width) X60cm(height) was bedded alternatively with four lanes of aeration pipes and heating pipes, and was insulated at three walls with 50mm styrofoam. Each aeration pipe of a diameter of 25mm had 4mm perforated holes at every 15cm longitudinally, and supplied air of about 2m$^3$/min to the composter to maintain aerobic condition . A stirrer rotating at 1 rpm made one round trip every 20 minutes on the conveying chain along the the length of the composter. Five tests (Test 1~Test 5) were implemented to evaluate the composting effectiveness of a sundry system with a horizontal bin-type composter. Treatments of two levels of the mixture ratio of swine manure and paper sludge cakes(manure : paper sludge cakes= 1 : 4 and 1 : 2) and two levels of the water content(W/C ; 70% and 50%) were made to test the significance of the physicochemical properties for decomposition of the mixture materials. Temperature, C/N ratio, water content, microbial activity of the composting materials were taken measurements to evaluate its performance with the lapse of composting time for tests. A small-scale sundry system with a bin-type composter did not appear to be an appropriate system for composting livestock manure. Since heat generation by the composting materials could not overcome heat loss due to areation in a small-scale composter, a proper thermal enviroment could not be maintained to propagate massively thermopilic microorganism relatively in a short period of time. Different from the result of Chol et al.(1992) 6), a temperature variation of the composting materials did not show the peak clearly and C/N ratio didn't lower with time as expected. Mesophilic microoragnism seemed to play an important role for decomposition of the mixture materials. A sundry system with a bin-type composter may be good for a large-scale livestock farm household which may produce enough animal manure. Therefore a decision should be made very carefully to choose a system for composting livestock waste.

• 생물환경조절학회지
• /
• 제3권2호
• /
• pp.136-144
• /
• 1994

A long- time aeration method was developed for purification of animal wastewater. Under repeated aereations of 4 hours on and 4 hours off, the higher removal rates were obtained which were in average of 99%, 96%, 92% and 50% for BOD, SS, total nitrogen and phosporous, respectively. In detail, the measured BOD concentrations of the influent and effluent were 2,700ppm and 40ppm while the SS concentrations in the primary chamber and of the effluent were about 3,000 and 110 ppm, respectively. Zeolite and activated carbon, applied for removing the nitrogen and phosphorous, showed a good absorption, especially zeolite for NH$_4$-N and activated carbon for NO$_3$-N and PO$_4$-P. The treatment cost per head by this method amounts to 1,923 won and it comes to 1.6% in the whole production cost. Therefore, this method is economically available with the half cost of the conventional activated sludge process.