• 방사선산업학회지
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• 제6권3호
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• pp.281-287
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• 2012

The model plant, Arabidopsis thaliana is the subject of an international genome research project. Massive doses of ionizing radiation have been shown to induce physiological changes in plants. The wild-type (Ler) Arabidopsis plants were irradiated with 100 Gy and 800 Gy of gamma-ray. Gibberellin (GA) affects developmental processes and responses according to the various environment conditions in diverse plant. The 13 GA isomers were analyzed at vegetative (VE) and reproductive (RE) stages by HPLC. Total GA contents were reduced with the increase in radiation doses at VE and RE stages. Specifically, levels of GA3, GA4, GA12, and GA34 were significantly reduced with the increase of radiation doses. Oligonucleotide microarrays analysis was performed with Arabidopsis plants at different developmental stages and doses of gamma-ray. Through the microarray data, we isolated 41 genes related to GA biosynthesis and signaling transduction. Expression of these genes was also decreased as the reduction of GA contents. Interestingly, in GA signaling related gene expression, gibberellin-responsive protein, putative (At2g18420) was down-regulated at VE and RE stages. Myb21 (At3g27810), Myb24 (At5g40350), and Myb57 (At3g01530) was down-regulated at RE stage. In GA biosynthesis related gene expression, YAP169 (At5g07200) and GA20ox2 (At5g51810) were down-regulated at 100 Gy treatment of VE stage and 800 Gy treatment of RE stage in cytoplasm, respectively. However, exceptively, GA3ox2 (At1g80340) was up-regulated at 100 Gy treatment of RE stage in cytoplasm. In this study, the wild type (Ler) Arabidopsis plants showed differences in response with development stage at the various doses of gamma-rays. GA contents change was reported in gamma irradiated plant.

• 방사선산업학회지
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• 제4권1호
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• pp.47-51
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• 2010

Centipedegrass is a warm season turfgrass in the world. Chlorogenic acid (CA) is one of the important compounds present in the leaf of centipedegrass and already known as an antioxidant, CA has become a key resistance against insect pests and bacteria pathogens of agricultural and horticultural plants during seedling stage. Furthermore, CA is accumulated by abiotic stress such as an UV irradiation. In present study, we investigated enhancement of the level of CA upon gamma irradiation in centipedegrass. The high performance liquid chromatography (HPLC) data analysis showed an approximately increasing of the CA levels from among the irradiated samples. However, plants irradiated at 50 Gy showed a constant increase in the CA level (0.0066 to $0.114mg\;ml^{-1}$ and 0.0258 to $0.2211mg\;ml^{-1}$, respectively) from $3^{rd}$ to $15^{th}$ day among one and three month irradiated plants compared to control. The present study, indicates an increase in the CA level upon gamma irradiation, suggests strategy for conferment of strong resistance in seedling stage plants by gamma irradiation as simplicity and cheaply method.

• Journal of Plant Biotechnology
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• 제3권1호
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• pp.1-5
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• 2001

Traditional culture technology of medicinal plants mainly depends on the field culture, which has many problems. With progress of modern culture technology, it has become possible to produce valuable secondary metabolites from medicinal plants. In this paper, we discuss about the pigment and saikosaponin production from too medicinal plants, Carthamus tinctorius and Bupleurum falcatum, through bioreactor culture system. A two-stage bioreactor culture system was established for the production of yellow and red pigments and saikosaponins by cell suspension cultures of Carthamus tinctorius and Bupleurum falcatum. In Carthamus tinctorius, balloon type airlift bioreactors and column type airlift bioreactors were employed for the tell culture and for the pigment production, respectively. The greatest pigment production was obtained on White medium supplemented with 4 mg/L kinetin, high levels of sucrose concentration and photosynthetic photon flux. In Bupleurum falcatum, adventitious roots were cultured in balloon type airlift bioreactors and the root growth was greatest on SH medium containing 5 mg/L IBA and 0.2 mg/L kinetin. HPLC analysis showed that the contents of main active saikosaponins a, c, and d in adventitious roots were almost the same as those in field cultured root.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.2174-2179
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• 2003

For many years, T/G Supplier has constructed a number of thermal power plants and researched to improve the performance and the reliability of steam turbine, which are achieved by advances in design and materials technology. In recent, interest is renewed in advanced steam condition as means of improving economy of thermal power plant and reducing environmental pollution. Improvements in the maximum power have been driven by the development of advanced rotor and bucket material and longer last stage bucket. Improvements in efficiency have been brought through advances in mechanical efficiency and thermodynamic efficiency. This paper describes a number of new steam path design features introduced to the steam turbine product. And also this paper describes new design technologies' development, new technologies' trend and technologies' development for ultra-super critical steam turbine.

• 한국초지조사료학회지
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• 제7권3호
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• pp.146-152
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• 1987

Sorghum cv. Pioneer 93 1, sorghum-sudangrass hybrid cv. Sioux and maize plant cv. Blizzard were assayed for toxic concentrations of nitrate-nitrogen ($NO_3$-N) and their relationship to morphological characteristics and environmental temperature in a field and phytotron trial. In the phytotron, sorghum and maize plants ranging from emergence to heading stage, were grown under different day/night temperatures of 30125, 25/20,28/18 and 1818 degree C. Nitrate-nitrogen in sorghum and maize plants was accumulated mainly in stems. Therefore nitrate concentration in the young plants was increased as development of stalks advanced and was highest at the stage of 3-4 leaves, when the plants had a leaf weight ratio 0.78-0.80 g/g plant weight. However, nitrate concentrations of the plant decreased as morphological development progressed, especially from the stage of growing point differentiation. Correlation coefficients showed a positive correlation of nitrate concentration with leaf weight ratio, leaf area ratio and specific leaf area, while plant height, dry matter percentage and absolute growth rate showed a negative association with TEX>$NO_3$-N ($P{\le}0.1$%). Cyanogenic glycosides, total nitrogen and crude protein were close associated with nitrate accumulation, and positively significant ($P{\le}0.1$%). High temperature over 30/25^{\circ}C.$ for 3 weeks increased N-uptake and dry matter accumulation, but reduced nitrate concentration. Under cold temperature below 18/8^{\circ}C.$ concentration of nitrate-N was increased in spite of its limited nitrogen uptake and plant growth.

• 시스템엔지니어링학술지
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• 제17권1호
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• pp.107-113
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• 2021

Plant Configuration Management(CM) is an activity to maintain consistency of design requirements, physical configuration, facility configuration information throughout the life cycle of the plant by systematically managing changes that occur during the plant design and operation process. Conformity between information must be ensured not only in the design stage, but also in the case of design changes in the operation and maintenance stages, and thus a computer system capable of efficiently managing them is required. In particular, in consideration of an application to small and medium-sized domestic plants, a computer system that can support configuration management at a low cost is needed. Accordingly, in this study, a configuration management system has been developed to support the management of plant design information and change procedures in the operation stage of small and medium-sized plants. Here, a case for quantitatively evaluating the effectiveness of the developed system is described.

• 열병합발전
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• 통권36호
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• pp.16-21
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• 2003

For many years, T/G Supplier has constructed a number of thermal power plants and researched to improve the performance and the reliability of steam turbine, which are achieved by advances in design and materials technology. In recent, interest is renewed in advance steam condition as means of improving economy of thermal power plant and reducing environmental pollution. Improvements in the maximum power have been driven by the development of advanced rotor and bucket material and longer last stage bucket. Improvements in efficiency have been brought through advance in mechanical efficiency and thermodynamic efficiency. This paper describes a number of new steam path design features introduced to the steam turbine product. And also this paper describes new design technologies' development, new technologies' trend and technologies' development for ultra-super critical steam turbine.

• 한국산림과학회지
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• 제85권3호
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• pp.347-359
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• 1996

이 연구(硏究)는 고속도로(高速道路) 절토(切土)비탈면에 1차적으로 도입된 식생의 생육상황(生育狀況)과 자연상태로 방치된 절토(切土)비탈면의 2차식생천이상황(次植生遷移狀況)을 파악하기 위하여 1989년과 1995년에 중부고속도로(中部高速道路)를 대상으로 실시되었다. 절토(切土)비탈면에 자연 침입하여 생육이 왕성한 초본류(草本類)는 참억새, 쑥, 솔새 등이었으며, 목본류(木本類)로는 산딸기, 아까시나무, 칡, 소나무 등이었다. 또한 녹화공법 적용 단계에서 파종된 식생중 외래목초류로는 Weeping lovegrass의 생육이 우세하였다. 절토(切土)비탈면에 평떼붙이기공법에 의해 적용된 잔디(Zoysia japonica)의 생육비율은 비탈면 조성후 유지관리(維持管理)가 수반되지 않은 상태에서는 현저하게 감소하거나, 침입식생에 의해 피압 소멸되었으며, 주변식생의 영향으로 천이가 진행되어 초본류(다년생초본(多年生草本), 1년생초본(年生草本))의 비율은 감소하였으며, 목본류(관목류(灌木類), 교목류(喬木類))의 출현비율이 증가하였다. 따라서 비탈면에 적용된 식생은 계속적인 유지관리(維持管理)가 수반되어야 하며, 녹화공법(綠化工法) 적용시 생활력(生活力)이 강한 초본류(草本類)(야생초화류(野生草花類) 포함) 및 목본류(木本類)의 도입이 요망된다.

• Plant Biotechnology Reports
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• 제1권1호
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• pp.19-25
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• 2007

To develop an efficient screening method for detection of the transgene in Chinese cabbage (Brassica rapa spp. pekinensis) utilizing Basta spray, optimal conditions for Basta application were examined in this study. Two transgenic Chinese cabbage lines were obtained through Agrobacterium-mediated transformation and used as transgenic positive controls in the Basta screening experiment. Differential concentrations of glufosinate-ammonium were sprayed into three different growth stages of 12 commercial Chinese cabbage cultivars. The results showed that no plants could survive higher than 0.05% glufosinate-ammonium, and plants at the 2-3 leaf stage were most vulnerable to glufosinate-ammonium. On the other hand, no damage was observed in the transgenic control plants. Reliability of the Basta spray method was proven by showing perfect co-segregation of the tolerance to glufosinate-ammonium and the presence of the bar gene in T₁ segregating populations of the transgenic lines, as revealed by both PCR and Southern blot analyses. Using the developed Basta screening method, we tried to investigate the transgene flow through pollen dispersal, but failed to detect any transgene-containing non-transgenic Chinese cabbages whose parents had been planted adjacent to transgenic Chinese cabbages in field conditions. However, the transgene was successfully detected using Basta spray from the non-transgenic plants bearing the transgene introduced by hand-pollination. Since the Basta spray method developed in this study is easy to apply and economical, it will be a valuable tool for understanding the mechanism of gene flow through pollen transfer and for establishing a biosafety test protocol for genetically modified (GM) Chinese cabbage cultivars.

• 한국환경농학회지
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• 제2권1호
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• pp.30-34
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• 1983

원자력시설(原子力施設)에서 방출(放出)될 수 있는 주요 핵종중(核種中)에서 $Cs^{137}$을 인위적으로 토양(土壤)에 처리(處理)하여 작물체(作物體)에 흡수(吸收), 이행(移行) 및 농축관계(濃縮關係)를 구명(究明)하고자 pot(토양(土壤)10㎏)당(當) $0.5{\sim}60{\mu}Ci$로 처리(處理)한 후 대두(大豆)를 재배(栽培)하여 다음과 같은 결과(結果)를 얻었다. 1) 공시(供試)된 $Cs^{137}$의 상기(上記) 처리농도(處理濃度)에서는 대두작물(大豆作物)의 생장저해(生長沮害) 영향을 볼 수 없었다. 2) 처리농도(處理濃度) 증가(增加)에 따라 대두작물(大豆作物)의 $Cs^{137}$흡수(吸收)는 증가(增加)하였으나 K함양(含量)은 감소(減少)하였으므로 두 이온간(間)의 길항성(拮抗性)을 보였다. 3) 생육시기별(生育時期別) 흡수량(吸收量)은 pod 형성기(形成期)까지는 증가(增加)하다 수확기(收穫期)에는 감소(減少)하는 경향을 보였고 종실(種實)에 비해 경엽부(莖葉部)에 높은 축적(蓄積)을 보였다. 4) 대두작물(大豆作物)의 $Cs^{137}$ 흡수률(吸收率)은 $0.069{\sim}0.005$의 범위로 $Cs^{137}$처리농도(處理濃度)에 따라서 감소(減少)하였고, 이행률(移行率)은 평균(平均) 38.6%였고, 종실(種實)에의 농축계수(濃縮係數) 또한 농도(濃度)의 증가(增加)에 따라 감소(減少)하였으며 $20{\mu}Ci$처리구(處理區)를 기준(基準)으로 할 경우 농축계수는 0.04 였다.