• Journal of Veterinary Clinics
• /
• v.22 no.3
• /
• pp.190-193
• /
• 2005

The concentration of tartrate resistant acid phosphatase (TRAP) in synovial fluid of normal stifle joint was investigated in order to establish a baseline data to distinguish between healthy joint and joint with injury of cranial cruciate ligament (CCL). Twenty three mixed-breed healthy dogs free from joint diseases (fourteen adult and nine young) were used in this study. The dogs were sedated and synovial fluid was collected from the femoropatellar compartment of stifle joints by direct arthrocentesis. The concentration of TRAP in synovial fluid was determined using the method of Lang. The concentration of TRAP were $0.083{\pm}0.039$ IU/ml in adult dogs, $0.064{\pm}0.023$ IU/ml in young dogs, $0.075{\pm}0.028$ IU/ml in large dogs (>22 kg), $0.076{\pm}0.046$ IU/ml in small dogs (<22 kg), $0.085{\pm}0.036$ IU/ml in neutered dogs and $0.056{\pm}0.022$ IU/ml in intact dogs. The concentration of TRAP in the neutered dogs was significantly (p<0.05) higher than the intact dogs. This data can be used baseline data for a comparison with joint with injury of cranial cruciate ligament.

• Journal of Veterinary Science
• /
• v.22 no.1
• /
• pp.3.1-3.13
• /
• 2021

Background: Multifidus muscle stiffness decreases in patients with lumbar intervertebral disk herniation; however, age-related changes in humans have not been reported. Objectives: The reliability of ultrasound shear wave elastography in dogs, and changes in the shear elastic modulus of the thoracolumbar multifidus muscle with aging in dogs, were investigated. Methods: Twelve beagle dogs were divided into 2 groups based on the age of onset of intervertebral disk herniation: young (aged not exceeding 2 years; 1.3 ± 0.6 years old, n = 5) and adult (4.9 ± 1.2 years old, n = 7). The shear elastic modulus of the multifidus muscle, from the thirteenth thoracic spine to the fourth lumbar spine, was measured using ultrasound shear wave elastography. The length, cross-sectional area and muscle to fat ratio of the multifidus muscle, and the grade of intervertebral disk degeneration, were assessed using radiographic and magnetic resonance imaging examinations. Results: The length and cross-sectional area of the multifidus muscle increased caudally. In the young group, the shear elastic modulus of the multifidus muscle of the thirteenth thoracic spine was less than that of the third lumbar spine. In the adult group, the shear elastic modulus of the multifidus muscle of first and third lumbar spine was lower than that of the same site in the young group. Conclusions: Ultrasound can be used to measure shear wave elastography of the thoracolumbar multifidus in dogs. If the multifidus muscle stiffness decreases, we should consider age-related change.

• Journal of Animal Science and Technology
• /
• v.66 no.2
• /
• pp.374-386
• /
• 2024

To predict the apparent total tract digestibility (ATTD) of crude protein (CP) in dogs we developed an in vitro system using an in vitro digestion method and a statistical analysis. The experimental diets used chicken meat powder as the protein source, with CP levels of 20% (22.01%, analyzed CP value as dry-based), 30% (31.35%, analyzed CP value as dry-based), and 40% (41.34%, analyzed CP value as dry-based). To simulate in vivo digestive processes a static in vitro digestion was performed in two steps; stomach and small intestine. To analyze ATTD the total fecal samples were collected in eight neutered beagle dogs during the experimental period. CP digestibility was calculated by measuring CP levels in dog food, in vitro undigested fraction, and dog feces. In result, CP digestibility at both in vivo and in vitro was increased with increasing dietary CP levels. To estimate in vivo digestibility the co-relation of in vivo ATTD and in vitro digestibility was investigated statistically and a regression equation was developed to predict the CP ATTD (% = 2.5405 × in vitro CP digestibility (%) + + 151.8). The regression equation was evaluated its feasibility by using a commercial diet. The predicted CP digestibility which was calculated by the regression equation showed high index of similarity (100.16%) with that of in vivo in dogs. With that, it would be a feasible non-animal method to predict in vivo CP digestibility by using in vitro digestion method and the proposed linear regression equation in adult dogs.

• Journal of Veterinary Clinics
• /
• v.26 no.4
• /
• pp.311-316
• /
• 2009

The purpose of this meta-analysis was to collect data from the literatures reporting prevalence of canine heartworm (HW) infection in an effort to emphasize the preventive importance of the disease and to serve as baseline data for further studies related to the disease management. Fourteen studies regarding the prevalence of HW infection published during the 1996-2007 were identified using the meta-analytic approach, involving a total of 1,790 cases by microfilarial test and 3,277 cases by enzyme-linked immunosorbent assay (ELISA) antigen test for adult worms. Data analysis was performed separately for pet and military dogs. The overall apparent prevalence of HW infection in pet dogs by microfilarial test and ELISA was 11.7% (183/1,563) and 16.5% (379/2,302), respectively (p < 0.001). No significant difference in prevalence by microfilaria and ELISA was observed between sexes whether assessed by data separately or pooled together. Compared to before 2000, the prevalence by microfilaria after 2000 has increased from 2.5% to 13.1% (p < 0.001), and the rate of adult worm has also increased from 9.9% to 20.3% (p < 0.001). The prevalence determined by ELISA was found to increase with age: 20.7% (n = 1,003) of dogs older than 2 years were positive, compared with 5.0% of dogs (n = 701) under 2 years of age (p < 0.001). Also, outdoor dogs showed significantly higher prevalence than indoor dogs (37.9% versus 3.4%; p < 0.001). For military dogs, the overall prevalence was 38.3% (87/227; 95% CI, 32.1 - 44.7%) by microfilarial test and 38.5% (375/975; 95% CI, 35.4 - 41.6%) by ELISA. This rate was significantly higher than that of pet dogs (p < 0.001). Occult infection rate of 37.5% and 66.7% was found in pet and military dogs, respectively.

• Korean Journal of Veterinary Research
• /
• v.55 no.4
• /
• pp.221-225
• /
• 2015

This study evaluated the effects of alfaxalone (3 mg/kg, intravenously) on echocardiographic examination in healthy dogs using echocardiography. Six adult Beagle dogs were used for this study. Left ventricular dimensions with systolic indexes, trans-blood flow at all cardiac valvular annulus and trans-mitral tissue Doppler values were measured from routine transthoracic echocardiography. Although the changes were not statistically significant, heart rate, left ventricular end-systolic diameter, left ventricular end-diastolic diameter, peak velocities of tricuspid A-wave and transpulmonic flow were increased after alfaxalone induction, while systolic blood pressure, fractional shortening, left ventricular ejection fraction, peak velocities of mitral E-wave, mitral A wave, tricuspid E-wave, transaortic flow and medial e'-, a'- and s'-peaks decreased after alfaxalone induction. No dogs showed hypoxemia during sedation, regardless of intubation and oxygen supply. Although alfaxalone showed mild cardiovascular depression, this protocol could be a good alternative sedative protocol for echocardiographic examination in healthy dogs because the cardiovascular depression was statistically and clinically insignificant. However, further studies in dogs with heart diseases should be conducted to confirm these findings after alfaxalone induction.

• Korean Journal of Veterinary Service
• /
• v.35 no.3
• /
• pp.207-213
• /
• 2012

The trunk conformations of the Gyeongju Donggyeongi (GD), Korean native dog was measured in the present study. Also, the information of reproductive activity was investigated in GD dogs. A total of 73 dogs were randomly selected out of 121 dogs in the Gyeongju County in July 2010, which were registered by inserting electronic chips in the withers. The hair colors of the GD were white, yellow, black and tiger brindle, the mean withers heights were $45.46{\pm}4.17cm$ and $49.28{\pm}3.56cm$ for female and male dogs, respectively The mean body length to withers height ratios were 117.63% and 116.4% for the female and male dogs. Concerning the reproductive capability of the GD, the mean litter number was $4.6{\pm}1.7$, the optimal mating time was $12.5{\pm}2.3$ days after the rutting season and the mean gestation period was $60.2{\pm}2.8$ days. The results of this study would be useful for compiling the official standard for the adult GD.

• Journal of Veterinary Clinics
• /
• v.39 no.2
• /
• pp.45-50
• /
• 2022

Vertebral left atrial size can be used as a radiographic tool to evaluate left atrial size in dogs. Vertebral left atrial size has been studied in dogs; however, few studies have been conducted on breed-specific differences in healthy dogs. To study the median vertebral left atrial size differences by breed and to investigate the association between age, sex, body condition score, thoracic depth-to-width ratio, and vertebral left atrial size. A total of 220 dogs of the following breeds: Maltese (n = 73), Beagle (n = 30), Poodle (n = 41), Shih-tzu (n = 44), and Mongrel (n = 32) were reviewed retrospectively. Sex, body weight, age, and body condition score of each dog were collected. Thoracic radiography was conducted for dorsoventral and right/left lateral views in all dogs to measure the vertebral heart score, vertebral left atrial size, and thoracic depth-to-width ratio. No significant differences in the median vertebral left atrial size were found among the breeds. There were no effects of sex, age, body condition score, and thoracic depth-to-width ratio on vertebral left atrial size. There was a significant positive correlation between the vertebral heart score and vertebral left atrial size. Breed, age, sex, and chest conformation did not correlate with vertebral left atrial size.

• Journal of Chest Surgery
• /
• v.27 no.5
• /
• pp.339-344
• /
• 1994

Sinus node dysfunction is common after orthotopic heart transplantation.Electrophysiologic studies have documented a high incidence [46% to 50%] of impaired sinus node automaticity and sinoatrial conduction in the early posttransplantation period. Sinus node dysfunction persists in over 20 % of patients and leads to prolonged bradyarrythmias, including sinus or nodal bradycardia and sinus arrest.The purpose of this paper was to observe sinus node dysfunction after orthotopic heart transplantation. Ten cardiac recipient dogs were monitored continuously after orthotopic transplantations between unrelated adult mongrel dogs. Crystalloid cardioplegic solution [Choongwoi Cardioplegia

• Korean Journal of Veterinary Research
• /
• v.20 no.2
• /
• pp.175-178
• /
• 1980

Normal values of serum electrolytes were determined for adult 36 females and 18 males, 1 to 6 years old, of Jindo dogs. The mean values, standard deviation and rangEs of sodium, potassium, chloride, calcium and inorganic phosphorus were studied and data analyzed to determine whether differences existed between the values of female and those of male dogs. The results obtained in this study were as follows: 1. The mean value of serum sodium was $143{\pm}1.5mEq/liter$, ranged from 138mEq/liter to 152 mEq/liter. The mean values for the females and males were $144{\pm}2.0mEq/liter$ and $142{\pm}1.7mEq/liter$, respectively. They showed that the female dogs had a tendency toward higher values than the male dogs, but no significant sex-related differences were observed. 2. The mean value of serum potassium was $4.6{\pm}0.2mEq/liter$, ranged frem 3.5mEq/liter to 6.0mEq/liter. The mean values for the females and males were $4.7{\pm}0.4mEq/liter$ and $4.5{\pm}0.3mEq/liter$. They showed that the female dogs had a tendency toward higher values than the male dogs, but sex differences were not found to be significant. 3. The mean value of serum chloride was $103{\pm}3.0mEq/liter$ ranged from 95mEq/liter to 117mEq/liter. The mean values for the females and males were $103{\pm}3.2mEq/liter$ and $103{\pm}3.3mEq/liter$, no sex differences showed. 4. The mean value of serum calcium was $5.2{\pm}0.1mEq/liter$ ranged from 4.7mEq/liter to 5.8mEq/liter. The mean values for the females and males were $5.4{\pm}0.1mEq/liter$ and $5.2{\pm}0.09mEq/liter$ respectively, no significant sexd iffere-nces were observed. 5. The mean value of serum inorganic phosphorus was $4.3{\pm}0.7mg/100ml$, ranged from 2.5mg/100ml to 5.8mg/100ml. The mean values for the females and males were $4.3{\pm}0.9mg/100ml$ and $4.1{\pm}0.8mg/100ml$, respectively, no sex differences showed.

• Parasites, Hosts and Diseases
• /
• v.54 no.6
• /
• pp.703-710
• /
• 2016

The trichomonad species Tritrichomonas foetus and Pentatrichomonas hominis were recently detected in the feces of dogs with diarrhea. However, little information is available on the prevalence and pathogenicity of these parasites in the canine population. Therefore, the aim of this study was to determine the prevalence and molecular characterization of trichomonads infecting pet dogs in Anhui and Zhejiang provinces, east China. In total, 315 pet dogs, with or without diarrhea, from 7 pet hospitals were included in this epidemiological survey. Microscopy and PCR detected P. hominis in 19.7% (62/315) and 31.4% (99/315) of fecal samples, respectively. T. foetus infection was detected in 0% (0/315) of samples with microscopy and in 0.6% (2/315) with PCR. The prevalence of P. hominis was significantly higher in young dogs (${\leq}12months$) than in adult dogs (>12 months), and was significantly higher in diarrheic dogs (50.6%) than in non-diarrheic dogs (24.3%; P<0.05). Infection with T. foetus did not correlate with any risk factors evaluated in this study. A sequence analysis of the P. hominis PCR products showed minor allelic variations between our sequences and those of P. hominis strains from other hosts in different parts of the world. Type CC1 was the most common strain in dogs in east China. The internal transcribed spacer 1 (ITS1)-5.8S rRNA gene sequences from the 2 T. foetus isolates detected in this study displayed 100% identity and were homologous to the sequences of other strains isolated from domestic cats in other countries.