• 성인간호학회지
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• 제26권1호
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• pp.58-66
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• 2014

Purpose: The aim of this study was to identify factors affecting the perception of sex life and sexual desire coping behavior among the spouseless elderly. Methods: The participants were 149 elders who resided in six administrative regions of B city. Data were collected from December 1, 2012 to March 3, 2013 using a questionnaire. Data were analyzed using descriptive statistics, t-test, ANOVA, Scheff$\acute{e}$ test, Pearson's correlation coefficient, and Stepwise multiple regression in a PASW 18.0 program. Results: Stepwise multiple regression analysis of data indicated that the most likely predictor is gender. Gender, sexual relation, subjective health status and parent-child relationship explained 34% of the variance of perception of sex life. Gender, dating status and age explained 44% of the variance of sexual desire coping behavior. Conclusion: Based on the study findings, gender and current dating status should be considered to conduct comprehensive sex health care education for healthy sex life of the elderly with no spouse.

• 대한간호학회지
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• 제36권7호
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• pp.1215-1223
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• 2006

Purpose: This study was done to understand elderly women within a community who have used lots of drugs. Method: Data was collected through in-depth interviews with four elderly women from April 2001 to February 2002. Data was analyzed by Giorgi's phenomenological method(2000). Results: The components in common with drug usage of elderly women were as follows: 'Awareness of the limitation of physical strength and uncertainty of life', 'Dread of a crisis situation occurring', 'Attachment and dependency on drugs', 'Expectation of a comfortable life and death', 'Prevention of family burden', and 'Pursuit of psychological comfort'. The general structure of experiences was as follows: causal factors were 'Awareness of uncertainty and limitation', 'dread of a crisis situation occurring' the results factor was 'Attachment and dependency on drugs' and the facilitating factors were 'Expectation of a comfortable life and death', 'Prevention of family burden', and 'Pursuit of psychological comfort'. The causal relation was cyclic. This structure showed a realistic adaptation pattern. Conclusion: This study contributed to provide fundamental data for nursing interventions for health promotion and promoting quality of life in elderly women by increasing understanding of the experiences of drugs use.

• 여성건강간호학회지
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• 제18권4호
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• pp.257-267
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• 2012

Purpose: This study was conducted to identify the caring experience of the mothers of childhood burn patients. Methods: Data were collected from 28 mothers of childhood burn patients, through semi-structured in-depth interviews. Data were categorized and coded by using content analysis. Results: 27 categories and 102 statements were drawn from 4 domains. The analyzed domains were psychological sufferings, confronted harsh reality, coping method, and future concerns. Psychological sufferings were categorized heartache, sense of guilt, confusion, regret, depression, sorriness, getting hurt, frustration and upset feeling. Confronted harsh nature were categorized economic difficulty, physical burn-out, lack of caring other children and family troubles. Coping method were categorized positive thinking, having hope, ventilating feelings, accepting the situations, demanding help from family members, changing patterns of the burned child rearing, collecting information for burn treatment, refusing accept the condition of burned child, avoiding personal and social relationship, and reliance on religion. Concerns were categorized concerns of growth and adaptation of the burned child, anxiety for scar, concerns of adapting school life and vague future concerns. Conclusion: The nursing interventions for early assessing psychological problems and providing social supports for caring both burn patients and other siblings should be provided to the mothers of childhood burn patients.

• Journal of Microbiology and Biotechnology
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• 제22권4호
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• pp.501-509
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• 2012

A 990 bp full-length gene (xynAHJ2) encoding a 329-residue polypeptide (XynAHJ2) with a calculated mass of 38.4 kDa was cloned from Bacillus sp. HJ2 harbored in a saline soil. XynAHJ2 showed no signal peptide, distinct amino acid stretches of glycoside hydrolase (GH) family 10 intracellular endoxylanases, and the highest amino acid sequence identity of 65.3% with the identified GH 10 intracellular mesophilic endoxylanase iM-KRICT PX1-Ps from Paenibacillus sp. HPL-001 (ACJ06666). The recombinant enzyme (rXynAHJ2) was expressed in Escherichia coli and displayed the typical characteristics of low-temperature-active enzyme (exhibiting optimum activity at $35^{\circ}C$, 62% at $20^{\circ}C$, and 38% at $10^{\circ}C$; thermolability at ${\geq}45^{\circ}C$). Compared with the reported GH 10 low-temperature-active endoxylanases, which are all extracellular, rXynAHJ2 showed low amino acid sequence identities (<45%), low homology (different phylogenetic cluster), and difference of structure (decreased amount of total accessible surface area and exposed nonpolar accessible surface area). Compared with the reported GH 10 intracellular endoxylanases, which are all mesophilic and thermophilic, rXynAHJ2 has decreased numbers of arginine residues and salt bridges, and showed resistance to $Ni^{2+}$, $Ca^{2+}$, or EDTA at 10 mM final concentration. The above mechanism of structural adaptation for low-temperature activity of intracellular endoxylanase rXynAHJ2 is different from that of GH 10 extracellular low-temperature-active endoxylanases. This is the first report of the molecular and biochemical characterizations of a novel intracellular low-temperature-active xylanase.

• 한국농림기상학회지
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• 제17권1호
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• pp.1-14
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• 2015

온실가스 증가로 인한 기후변화는 농업 생태계에 다양한 경로로 영향을 미쳐 작물 생산에 영향을 미칠 수 있다. 또한, 농업 생태계는 생물, 기후, 토양 및 경제 환경이 서로 복잡하게 연결되어 있어 개별 분야에 초점을 맞춘 적응 대책들은 농업 부문 내 다른 영역에 의도하지 않은 파급 효과를 초래할 수 있다. 기후변화 조건에서 복잡한 농업 생태계의 상호작용을 고려하면서 최적의 작물 생산성을 유지하기 위해 개별분야별 모델을 연계한 통합 예측 시스템 구축이 요구된다. 이러한 통합시스템을 구축하기 위해서는 단계적 접근이 필요하다. 국내에서 사용되고 있는 모델들은 통합시스템에 적합하도록 설계된 것이 아니기 때문에, 이를 위한 모델의 재개발이 필요하다. 농업생태계 감시를 위한 수퍼사이트와 위성사이트의 구축을 통해 장기간 작물 생육 자료를 확보하고 이를 개별 분야 모델의 개선에 활용할 수 있다. 모델 대상의 추상화와 상속과정을 통해 보다 유연한 형태의 통합 모델의 모듈 개발이 가능할 것이다. 마지막으로, 농업분야는 사회경제적인 요인에 지대한 영향을 받기 때문에, 농업생산과 경제분야가 연계될 수 있는 통합 시스템 구축이 바람직 할 것 이다.

• 한국간호교육학회지
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• 제12권1호
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• pp.78-85
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• 2006

Objectives: College students experiences more stress than when they were in high school. The students in nursing college, experience a variety of stress related to clinical practices, the examination for registered nurse license etc. The purpose of this study was to identify the factors that affect the health promotion behavior among the students in a nursing college based on SAT(Social Action Theory). Methods: The study was a cross-sectional design with a convenience sample of 427 students recruited from April to May in 2005. SAT was utilized for the theoretical framework of the study. The instruments used were Profile of Mood Stress, Life Stress Scale for college student, Social Adaptation Scale, Health Knowledge and Health Promotion Lifestyle Profile. Data were analyzed with SPSS 12.0 program. Results: The study showed that grades, moods, and satisfaction of relationship with friends, satisfaction with pocket money made up 29.0% of significant factors related to health promotion behavior among the college students in nursing. Conclusion: It suggests that the known factors need to be taken into consideration when developing the health promotion program for college students in nursing.

• 성인간호학회지
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• 제26권6호
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• pp.693-702
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• 2014

Purpose: The purpose of this study was to examine the relationships between treatment belief, personal control, depressive mood, and health-related quality of life in patients with hemodialysis based on self-regulation theory. Methods: Data were collected from 220 patients at 27 local hemodialysis clinics in Seoul during 2013 and 2014. The Revised Illness Perception Questionnaire, the Hospital Anxiety and Depression Scale, and Medical Outcomes Study Short Form-12 were used to measure outcome variables. Data were analyzed using descriptive statistics, t-tests, ANOVA, Pearson correlation, and multiple regression using the 'enter' method. Results: Treatment belief and personal control scored 3.58 and 3.54 out of 5 points respectively, on average. Treatment belief and personal control of kidney disease were negatively correlated with depressive mood and positively correlated with health-related quality of life. According to the regression analysis, treatment belief, monthly income, and personal control were discovered to account for 21.8% of the variance in depressive mood, where as depressive mood, monthly income, treatment belief, and age were found out to account for 40.6% of the variance in health-related quality of life. Conclusion: Our study demonstrated significant positive relationships between treatment belief and illness outcome in hemodialysis patients. Interventions aimed to provide the necessary information and trust to maximize the effectiveness of treatment need be developed to improve patients outcomes.

• 종양간호연구
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• 제12권3호
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• pp.237-245
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• 2012

Purpose: The purpose of this study was to identify attributes of the concept of cancer survivorship. Methods: Walker & Avant's concept analysis framework (2005) was used to review the medical & nursing text books, medical and nursing research articles related to cancer survivorship and published from 1985 to 2011. Results: There were nine aspects of the concept of cancer survivorship: A process, liminality, uncertainty, life-changing experience, complexity, unique experience, duality of positive and negative aspects, partnership, and need for support. Antecedents of cancer survivorship were cancer diagnosis and perception as a cancer survivor. Empirical referents of cancer survivorship were survival rate, quality of life, adaptation to the survivorship experience, and health-related hardiness, social support, spirituality, and health behavior. The consequences of cancer survivorship were effects on the physical, psychosocial, spiritual and socio-economic well-being of the patients. Conclusion: This concept analysis of cancer survivorship is expected to contribute to promotion of survivorship care in the clinical field by removing conceptual ambiguity and confirming the true meaning of survivorship care.

• Mycobiology
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• 제37권3호
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• pp.161-170
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• 2009

Cryptococcus neoformans is a basidiomycete human fungal pathogen that causes meningoencephalitis in both immunocompromised and immunocompetent individuals. The ability to sense and respond to diverse extracellular signals is essential for the pathogen to infect and cause disease in the host. Four major stress-activated signaling (SAS) pathways have been characterized in C. neoformans, including the HOG (high osmolarity glycerol response), PKC/Mpk1 MAPK (mitogen-activated protein kinase), calcium-dependent calcineurin, and RAS signaling pathways. The HOG pathway in C. neoformans not only controls responses to diverse environmental stresses, including osmotic shock, UV irradiation, oxidative stress, heavy metal stress, antifungal drugs, toxic metabolites, and high temperature, but also regulates ergosterol biosynthesis. The PKC(protein kinase C)/Mpk1 pathway in C. neoformans is involved in a variety of stress responses, including osmotic, oxidative, and nitrosative stresses and breaches of cell wall integrity. The $Ca^{2+}$/calmodulin- and Ras-signaling pathways also play critical roles in adaptation to certain environmental stresses, such as high temperature and sexual differentiation. Perturbation of the SAS pathways not only impairs the ability of C. neoformans to resist a variety of environmental stresses during host infection, but also affects production of virulence factors, such as capsule and melanin. A drug(s) capable of targeting signaling components of the SAS pathway will be effective for treatment of cryptococcosis.

• Mycobiology
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• 제46권4호
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• pp.361-369
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• 2018

The rice blast fungus, Magnaporthe oryzae, is an important pathogen of rice plants. It is well known that genes encoded in the genome have different evolutionary histories that are related to their functions. Phylostratigraphy is a method that correlates the evolutionary origin of genes with evolutionary transitions. Here we applied phylostratigraphy to partition total gene content of M. oryzae into distinct classes (phylostrata), which we designated PS1 to PS7, based on estimation of their emergence time. Genes in individual phylostrata did not show significant biases in their global distribution among seven chromosomes, but at the local level, clustering of genes belonging to the same phylostratum was observed. Our phylostrata-wide analysis of genes revealed that genes in the same phylostratum tend to be similar in many physical and functional characteristics such as gene length and structure, GC contents, codon adaptation index, and level of transcription, which correlates with biological functions in evolutionary context. We also found that a significant proportion of genes in the genome are orphans, for which no orthologs can be detected in the database. Among them, we narrowed down to seven orphan genes having transcriptional and translational evidences, and showed that one of them is implicated in asexual reproduction and virulence, suggesting ongoing evolution in this fungus through lineage-specific genes. Our results provide genomic basis for linking functions of pathogenicity factors and gene emergence time.