• Journal of Nutrition and Health
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• 제36권4호
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• pp.376-381
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• 2003

본 연구에서는 흰쥐 간장에 있어서 아실-CoA 합성효소 4의 세포내 소기관의 존재 여부를 확인함과 동시에 fasting, high fat diet, fat-free high sucrose diet, 퍼옥시솜 증식 인자인 DEHP [Di-(2-ethylhexyl)phthalate]를 급여한 흰쥐 간장에 있어서 ACS4의 발현에 대하여 조사하였다. ACS4는 ACSI과 마찬가지로 흰쥐 간장의 마이크로솜, 미토콘드리아와 퍼옥시솜에 존재하는 것으로 생각되며 미토콘드리아에서 가장 많은 단백질이 검출되었다. ACS4 mRNA는 절식하였을 때와 high fat diet, fat-free high sucrose diet을 급여하였을 때는 대조군에 비하여 2.3배 발현이 증가하였으며 DEHP을 급여하였을 때는 3.9배 mRNA의 증가를 나타내었다. 이러한 결과를 종합하여 보면 간장에 있어서 ACS4는 기본적인 $\beta$-산화뿐만 아니라 호르몬에 의한 조절과 간접적으로는 인슐린에 의한 조절도 받는 것으로 생각되며 다양한 기능을 수행하고 있음을 추측할 수 있다.

• Bulletin of the Korean Chemical Society
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• 제35권7호
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• pp.2065-2071
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• 2014

${\beta}$-Secretase (beta-amyloid converting enzyme 1 [BACE1]) is involved in the first and rate-limiting step of ${\beta}$-amyloid ($A{\beta}$) peptides production, which leads to the pathogenesis of Alzheimer's disease(AD). Therefore, inhibition of BACE1 activity has become an efficient approach for the treatment of AD. Ligand-based and docking-based 3D-quantitative structure-activity relationship (3D-QSAR) studies of acyl guanidine analogues were performed with comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) to obtain insights for designing novel potent BACE1 inhibitors. We obtained highly reliable and predictive CoMSIA models with a cross-validated $q^2$ value of 0.725 and a predictive coefficient $r{^2}_{pred}$ value of 0.956. CoMSIA contour maps showed the structural requirements for potent activity. 3D-QSAR analysis suggested that an acyl guanidine and an amide group in the $R_6$ substituent would be important moieties for potent activity. Moreover, the introduction of small hydrophobic groups in the phenyl ring and hydrogen bond donor groups in 3,5-dichlorophenyl ring could increase biological activity.

• 대한유전성대사질환학회지
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• 제15권1호
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• pp.35-39
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• 2015

중쇄 acyl-CoA 탈수소효소 결핍증은 미토콘드리아에 존재하는 효소 중 하나인 MCAD의 부족으로 인하여 적절한 지방산 산화가 이루어지지 못하는 대사질환으로 지방산 산화와 관련된 대사 질환 중 가장 흔한 형태이다. 다양한 임상증상으로 저혈당, 발달지연, 발작, 돌연사 등이 나타날 수 있다. 저자들은 신생아 선별검사상 C6, C8, C10:1 acylcarnitine, C8/C2 ratio 혹은 C8/C10 ratio의 증가를 보이는 무증상의 신생아에서 유전자 분석검사를 통해 MCAD 결핍증을 진단하였다. 생후 10개월 경, 고열을 동반한 전신 강직성간대경련 발생하였으나 혈액검사 상 저혈당은 관찰되지 않았고 발열 호전된 후 추가적인 경련은 없었다. 이후 생후 25개월까지 추적관찰 하였을 때 경련을 포함한 증상 없었고, 정상적인 성장과 발달을 보였다. 무증상의 신생아에서 신생아 선별검사를 통해 우연히 MCAD 결핍증으로 진단된 후 1회의 열성경련 발생하였으나 대사성 위기없이 정상적인 성장 및 발달을 보이고 있는 환아가 있어 이에 증례를 보고하는 바이다.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2003년도 제3회 발생공학 국제심포지움 및 학술대회
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• pp.96-96
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• 2003

• Clinical and Experimental Pediatrics
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• 제59권sup1호
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• pp.45-48
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• 2016

Short-chain acyl-CoA dehydrogenase deficiency (SCADD) is a rare autosomal recessive mitochondrial disorder of fatty acid ${\beta}$-oxidation, and is associated with mutations in the acyl-CoA dehydrogenase (ACADS) gene. Recent advances in spectrometric screening for inborn errors of metabolism have helped detect several metabolic disorders, including SCADD, without symptoms in the neonate period. This allows immediate initiation of treatment and monitoring, so they remain largely symptomless metabolic disease. Here, we report a 15-month-old asymptomatic male, who was diagnosed with SCADD by newborn screening. Spectrometric screening for inborn errors of metabolism 72 hours after birth revealed an elevated butyrylcarnitine (C4) concentration of $2.25{\mu}mol/L$ (normal, < $0.99{\mu}mol/L$). Urinary excretion of ethylmalonic acid was also elevated, as detected by urine organic acid analysis. To confirm the diagnosis of SCADD, direct sequencing analysis of 10 coding exons and the exon-intron boundaries of the ACADS gene were performed. Subsequent sequence analysis revealed compound heterozygous missense mutations c.164C>T (p.Pro55Leu) and c.1031A>G (p.Glu344Gly) on exons 2 and 9, respectively. The patient is now growing up, unretarded by symptoms such as seizure and developmental delay.

• 청정기술
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• 제11권4호
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• pp.195-204
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• 2005

기존 공정에 비하여 오염물 발생이 적고 수율이 60 % 이상의 환경친화적인 새로운 N-acyl taurate 단일축합공정을 개발하였다. 개발된 공정의 최적 운전조건은 석유계 정제유인 parasol 123 용매와 반응촉매로 sulfone산계 촉매와 인산계 촉매의 혼합촉매를 사용할 때 반응온도가 $210{\sim}230^{\circ}C$이며 반응시간이 6 ~ 9시간 이었다. 이렇게 제조된 제품은 기존 수입된 N-acyl taurate와 비교하여 산가, 아민가, 색상 등의 물성이 비슷하거나 더 좋고 부산물도 적게 배출되어 본 연구에서 개발된 공정이 기존 공정보다 우수한 공정이라고 할 수 있다. 그리고 본 연구에서 제조된 N-acyl taurate를 시판 음이온 계면활성제와 표면장력, 기포력, 기포안정성, 경수안정성, 유화력 등의 물성을 비교할 때 동등 이상의 물성을 보여 기존의 음이온 계면활성제를 충분히 대체할 수 있음을 확인할 수 있었다.

• Archives of Pharmacal Research
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• 제26권9호
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• pp.716-718
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• 2003

In the course of our search for Acyl-CoA: cholesterol acyltransferase (ACAT) inhibitors from natural sources, a new type of ACAT inhibitor was isolated from a methanol extract of Diospyros kaki. On the basis of spectral and structural evidence, the compound was identified as pheophorbide A-methyl ester. Pheophorbide A-methyl ester inhibited ACAT activity in a dose dependent manner with an $IC_{50}$ value of 1.85 $\mu$ g/mL.

• Journal of Microbiology and Biotechnology
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• 제32권7호
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• pp.949-954
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• 2022

The lipolytic yeast Candida aaseri SH14 contains three Acyl-CoA oxidases (ACOXs) which are encoded by the CaAOX2, CaAOX4, and CaAOX5 genes and catalyze the first reaction in the β-oxidation of fatty acids. Here, the respective functions of the three CaAOX isozymes were studied by growth analysis of mutant strains constructed by a combination of three CaAOX mutations in minimal medium containing fatty acid as the sole carbon source. Substrate specificity of the CaAOX isozymes was analyzed using recombinant C. aaseri SH14 strains overexpressing the respective genes. CaAOX2 isozyme showed substrate specificity toward short- and medium-chain fatty acids (C6-C12), while CaAOX5 isozyme preferred long-chain fatty acid longer than C12. CaAOX4 isozyme revealed a preference for a broad substrate spectrum from C6-C16. Although the substrate specificity of CaAOX2 and CaAOX5 covers medium- and long-chain fatty acids, these two isozymes were insufficient for complete β-oxidation of long-chain fatty acids, and therefore CaAOX4 was indispensable.

• Journal of Applied Biological Chemistry
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• 제49권2호
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• pp.57-61
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• 2006

Isoflavones 1-3 and pterocarpans 4-8 were isolated from methanol extract of roots of Glycine max. In inhibitory effect against human acyl-CoA:cholesterol acytransferase (ACAT)-1 and ACAT-2, glyceollin I 5 showed potent hACAT-1 ($IC_{50}=299.0{\mu}M$) and hACAT-2 ($IC_{50}=82.7{\mu}M$) inhibitory activities.

• Journal of Ginseng Research
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• 제9권1호
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• pp.112-118
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• 1985

It has been known that the ginseng extracts activate the lipid metabolism in animal body. The experiments were undertaken to elucidate the effects of total, diol, and trial saponin of ginseng on the activities of acyl Co-A synthetase and hydroxyacyl Co-A dehydrogenase involved in fatty acid metabolism in normal albino rat liver. The acyl Co-A synthetase activity, in vitro, was increased by 20% with treatment of 2.5${\times}$10-3% total saponin, by 14% with 2.5${\times}$10-3% diol saponin, arid 30% with 2.5${\times}$10-4% triol saponin, respectively. And the enzyme activity was increased by 27% at 2 hours after intraperitoneal injection of total saponin. Hydroxyacyl Co-A dehydrogenase activity, in vitro, was increased by 77% with 10-4% total saponin, by 64% with 10-2% diol saponin, and by 72% with 10-3% triol saponin, respectively. Also, the enzyme activity, in vivo, was increased by 15.3% and 33% at 2 hours and 4 hours.