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Functional Studies of Acyl-CoA Synthetase 4 in the Rat Liver  

정영희 (전남대학교 농업생명과학대학 동물자원학부)
문승주 (생물공학연구소)
강만종 (농업과학기술연구소)
Publication Information
Journal of Nutrition and Health / v.36, no.4, 2003 , pp. 376-381 More about this Journal
Abstract
Acyl-CoA synthetase 4 (ACS4) is an arachidonate-preferring enzyme abundant in steroidogenic tissues. We examined ACS4 in rat liver, which contains a variety of pathways that use acyl-CoAs, in order to determine subcellular locations. We demonstrate that ACS4 protein was present most abundantly in the mitochondria and to a much lesser extent in the peroxisomes and microsomes. To determined the dietary effects on the level of ACS4 mRNA, northern blotting was carried out using total RNA from the livers of adult male rats fed various diets. Fasting, high fat diet, and fat-free high sucrose diet increased the hepatic level of ACS4 mRNA approximately 2-fold. Furthermore, the levels of ACS4 mRNA were induced by DEHP[Di- (2-ethylhexyl) phthalate]. These data suggest that ACS4 expression in the liver is regulated with a variety of pathways, including $\beta$-oxidation, hormone, and insulin.
Keywords
acyl-CoA synthetase; arachidonic acid; subcellular fractionation; northern blotting; western blotting; fasting; high fat diet; fat-free high sucrose diet; DEHP[Di-(2-ethylhexyl) phthalatel;
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