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Pharmacokinetic Preformulation Study of rH IL-2 (인터루킨-2의 제제설계를 위한 체내 동태학적 연구)

  • Seo, Min-Seok;Shim, Chang-Koo;Kwon, Jong-Bum;Na, Do-Sun;Lee, Sun-Bok;Hahm, Kyung-Soo;Han, Moon-Hi
    • YAKHAK HOEJI
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    • v.34 no.4
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    • pp.238-243
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    • 1990
  • Pharmacokinetic characteristics of recombinant human interleukin-2 (rH IL-2) wre studied in the rat. First, different doses of rH IL-2 ranging from 6,400 to 1,600,000 U/kg were injected intravenously and the effect of dose size on the pharmacokinetics was examined. There was no dose dependency in the pharmacokinetics of rHIL-2 in the dose range of 6,400-40,000 U/kg. But at the dose of 1,600,000 U/kg, there was a severe hemolysis throughout the experiment and the pharmacokinetic parameters such as Vdss and CLt were significantly increased compared to those obtained from lower doses. It also showed that this drug is hardly distributed to the peripheral tissues and hardly eliminated from the body, since the valume of distribution (Vdss) and total body clearance (CLt) were 45-75 ml/kg and 1-2 ml/min/kg, respectively. The Vdss is close to the actual plasma volume and the CLt is less than glomerular filtration rate (GFR). Therefore it seemed that rH IL-2 is distributed only in the plasma pool and hardly filtered in the kidney due to its very large molecular weight. Second, rH IL-2 was administered to the rat via several routes such as hepatic portal vein (PV), intraperitoneal (IP), peroral (PO) and intranasal (IN) routes. The bioavailabilities (BA) of PV, IP, PO and IN routes were 96.8, 4.9, 0 and 0.1%, respectively. The addition of some nasal absorption enhancers such as taurocholate, taurodeoxycholate, glycocholate and glycodeoxycholate did not increase the BA of intranasaly administered rH IL-2. The result is contrast to the effect of these bile salts on the nasal absorption of ${\alpha}-inteferon$. Considering it together with the pharmacokinetic parameters, very large molecular weight of rH IL-2 seemed again to be the cause to very poor membrane permeability.

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Physiological Responses and Subjective Sensations of Human Wearing Soccer Wear of Different Materials and Designs (축구복 소재와 디자인이 인체생리반응과 주관적 감각에 미치는 영향)

  • Choi Jeong-Wha;Kim So-Young;Jeon Tae-Won
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.1 s.139
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    • pp.35-45
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    • 2005
  • The purpose of this study was to evaluate thermal properties of soccer wear with different materials and designs. As a beginning step, the questionnaire survey about the actual condition of soccer wears was conducted. with the results of the questinnaire, two soccer wears with new material and design that were improved in tactile sensations, absorption and ventilation were developed. We evaluated thermal and subjective responses of subjects wearing Korea national soccer team uniform in 1998 World Cup (Uniform 98), soccer wear with new material and same design(New II) and with new material and new design(New I). New I was made with mesh in armhole for improving ventilation. Rectal temperature, skin temperature, clothing microclimate, and heart rate were measured in climatic chamber test(twelve times) and field test(eighteen times). The results were as follows. 1. As the results of the climatic chamber test, rectal temperature was lower in New I and New II than Uniform98, and mean skin temperature was lower in New I than Uniform 98 and New II. Heart rate was lower in New I than New II, and total body weight loss and local sweating were not significantly different by soccer wears. 2. As the results of the field test, rectal temperature was lower in New I than Uniform98 and New II. Mean skin temperature was lower in New II than Uniform98 and New I. Clothing microclimate temperature was lower in New II than Uniform98 and New 1, and clothing microclimate humidity was lower in New I, New II than Uniform 98. Heart rate was lower in New I than Uniform 98, New II and total body weight loss and local sweating were lower in New I, New II than Uniform 98. In conclusion, New I using new design using mesh in armhole and new material using sweat absorbent finishing was excellent from the point of view of physical responses, ventilation and sweat absorption.

Effect of Water Hyacinth (Eichhornia Crassipes) Silage on Intake and Nutrient Digestibility in Cattle Fed Rice Straw and Cottonseed Cake

  • Tham, Ho Thanh;Uden, Peter
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.5
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    • pp.646-653
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    • 2013
  • Four crossbred Sindhi heifers with an average body weight (BW) of 135 kg and a mean age of 17 months were used to investigate the effect of feeding different combinations of rice straw and ensiled water hyacinth (EWH) supplemented with a source of protein in the form of cottonseed cake (CSC) on intake and digestibility. Four treatments consisting of graded levels of EWH were arranged in a $4{\times}4$ Latin square. The levels of EWH were set at: 0 (EWH0), 15 (EWH15), 30 (EWH30), and 45% (EWH45) of an expected total dietary dry matter (DM) intake of 30 g total DM per kg BW per day. Rice straw was offered ad libitum, while CSC was given at a fixed level of 5 g DM/kg body weight (BW). Voluntary intake and digestibility were measured consecutively in the 4 experimental periods which each lasted 28 days. The crude protein (CP) content of EWH, rice straw and CSC were 174, 53 and 370 g/kg DM, respectively. Rice straw had the highest neutral detergent fibre (NDFom) content (666 g/kg DM), followed by EWH (503 g/kg DM) and the lowest content was 418 g/kg DM in the CSC. The actual EWH contents in the consumed diets were 0, 17, 32 and 52% for EWH0, EWH15, EWH30 and EWH45, respectively. Rice straw intake decreased with level of EWH offered from 3049 for EWH0 to 1014 g/day for EWH45. Crude protein intake was 16, 25 and 33% higher (p<0.001) in EWH15, EWH30 and EWH45 treatments, respectively, as compared to EWH0. Digestibility of organic matter (OM), CP, NDFom and acid detergent fibre (ADFom) increased with increasing level of EWH offered. The highest OM digestibility (72.2%) was found for treatment EWH45 and the lowest (47.4%) for treatment EWH0. In spite of similar dietary CP contents, CP digestibility increased by 21 (EWH15), 31 (EWH30) and 40% (EWH45) with increasing level of EWH in comparison with treatment EWH0. It is concluded that increasing level of EWH in cattle diets considerably improved CP intake and digestibility of nutrients.

Determining Relative Weights of Criteria for Evaluating National Quarantine Station by the Analytic Hierarchy Process (AHP방법을 적용한 국립검역소 평가 준거의 가중치 결정)

  • Jeong, Mun-Yong;Lee, Moo-Sik;Kim, Dae-Kyung;Yoo, In-Sook
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.1
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    • pp.335-340
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    • 2010
  • In accordance with the increasing possibility that the new and reoccurring epidemics continuously appearing abroad flow into Korea, it is very important to evaluate the efficiency of quarantine management projects of National Quarantine Station. This study adopted the pairwise comparison approach using the analytical hierarchy process(AHP) that has been recognized with its scientific adequacy for allocating the weight, the relative importance of evaluation components. AHP technique is evaluated to be distinguished in measuring the impact by making hierarchy of, simplifying and systemizing the complicated multi-criteria decision making problems. The weights by evaluation indexes were 0.2 in the organization evaluation field and 0.8 in the service and program field. The quarantine business showed the highest value, 0.45, in the service and program field. The indexes were classified into the upper category (organization evaluation field, service and program evaluation field) and lower category (input/process/result/quarantine field, inspection field, hygiene management field, pathogenic organ investigation and monitoring field, epidemics prevention promotion/education field). The evaluation indexes that were finally selected were applied to the actual businesses in National Quarantine Station. Next, the minimum evaluation indexes were selected and so the evaluation system on the businesses in National Quarantine Station was more systemized.

Computational Approach for the Trade-Off Study between the Total Cost and the Member Connections in Steel Frames (강 뼈대구조물의 총 경비와 부재연결과의 상반관계에 관한 연구)

  • Choi, Byoung Han;Lim, Jung Hwan
    • Journal of Korean Society of Steel Construction
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    • v.19 no.1
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    • pp.15-27
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    • 2007
  • Over the past decade, labor costs have increased relative to the cost of material hardware according to analysts in the construction industry. Therefore, the minimum weight design, which has been widely adopted in the literature for the optimal design of steel structures, is no longer the most economical construction approach. Presently, although connection- related costs is crucial in determining the most cost-effective steel structures, most studies on this subject focused on minimum-weight design or engaged in higher analysis. Therefore, in this study, we proposed a fabrication scheme for the most cost-effective moment-resisting steel frame structures that resist lateral loads without compromising overall stability. The proposed approach considers the cost of steel products, fabrication, and connections within the design process. The optimal design considered construction realities, with the optimal trade-off between the number of moment connections and total cost was achieved by reducing the number of moment connections and rearranging them using the combination of analysis that includes shear, displacement and interaction value based on the LRFD code and optimization scheme based on genetic algorithms. In this study, we have shown the applicability and efficiency in the examples that considered actual loading conditions.

Influence of Benzocaine as an Anaesthetic for the Grading of Tilapia (Tilapia 선발을 위한 Benzocaine의 영향)

  • Kim, Hyung-Joo;Chun, Seh-Kyu
    • Journal of fish pathology
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    • v.2 no.2
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    • pp.99-108
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    • 1989
  • Anaethetics are needed for handling fish, especially for transportation, tagging and grading. Among them, MS-222 has been popular in aquaculture since it has an excellent anaesthetic effect. However, MS-222 is more expensive than other chemicals. Benzocaine (Ethyl-p-aminobenzoate) has a similar molecular formula and equivalent anaesthetic effect to MS-222, and is cheaper. The purpose of this study was first to compare anaesthetic effects (Benzocaine) under various conditions : temperature, concentration, pH and body weight. Second purpose was to compare actual anaesthetic effects at 50 ppm benzocaine at ambient temperature and pH for the grading of tilapia. The results of this study are as follows : 1. The effect of anaesthesia at $24^{\circ}C$ was better with low pH, that is 5.6 than high pH 6.6 and 7.6. 2. The anaesthetic effect was not different at different body weight form 11g to 1,350g. 3. The fish were anaesthetized in 4~10 minutes at 50 ppm benzocaine at temperature. $20{\sim}24^{\circ}C$ and pH 6.8~7.3 and recovered in 4~6 minutes when they were put back in the fresh water after 30 minutes anaesthesia. 4. Benzocaine was more sensitive at pH fluctuation than temperature. 5. Twenty four hour-TLm of Benzocaine was 50 ppm at $24^{\circ}C$, pH 6.8 when the fish were put back in the fresh water after 120 minutes.

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Analysis of Broken Rice Separation Efficiency of a Laboratory Indented Cylinder Separator

  • Kim, Myoung Ho;Park, Seung Je
    • Journal of Biosystems Engineering
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    • v.38 no.2
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    • pp.95-102
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    • 2013
  • Purpose: Using a laboratory indented cylinder separator, broken rice separation experiments were conducted and the characteristics of the separation process were studied to provide information for developing a prototype indented cylinder broken rice separator. Methods: Rice (Ilmi variety) milled in a local RPC was used for the experiment. Rice kernels were classified into four groups according to their length l; whole kernels (I > 3.75 mm), semi-whole kernels (2.5 < I < 3.75 mm), broken kernels (1.75 < I < 2.5 mm), and foreign matters (I < 1.75 mm). A laboratory grain cleaner, Labofix '90 (Schmidt AG, Germany) was used for the experiments. Experiments were designed as a $4{\times}4$ factorial arrangement in randomized blocks with three replications. Cylinder rotational speeds (17, 34, 51, 68 rpm) and trough angles (15, 37.5, 60, $82.5^{\circ}$) were the two factors and feed rates (25, 50 kg/h), indent shapes (Us, $S_1$ type), and indent sizes (2.5, 3.75 mm) were treated as the blocks. Two 125 g samples and one 125 g sample were taken at the cylinder outlet and from the trough, respectively. The whole, semi-whole, and broken kernel weight ratio of the samples and feed was determined by a rice sizing device. From these weight ratios, purities, degrees of extraction and coefficient of separation efficiency were calculated. Results: Trough angle, cylinder speed, and their interaction on the coefficient of separation efficiency were statistically significant. Cylinder speed of 17, 34, and 51 rpm made the most effective separation when the trough angle was $15^{\circ}$ or $37.5^{\circ}$, $60^{\circ}$, and $82.5^{\circ}$, respectively. Maximum values of coefficient of separation efficiency were in the range of 60 to 70% except when the indent size was 2.5 mm and were recorded for the combinations of low cylinder speed (17 rpm) with medium trough angle ($37.5^{\circ}$ or $60^{\circ}$). Indent shape did not appear to make any noticeable difference in separation efficiency. Conclusions: Due to the interaction effect, the trough angle needs to be increased appropriately when an increase in cylinder speed is made if a rapid drop of effectiveness of separation should be avoided. In commercial applications, $S_1$ type indents are preferred because of their better manufacturability and easier maintenance. For successful separation of broken kernels, the indent size should be set slightly bigger than the actual sizes of broken kernels: an indent size of 3.0 mm for separating broken kernels shorter than 2.5 mm.

A Study on Development of Common Criteria for Evaluation of Internet Ethics Index (인터넷윤리 지수의 평가를 위한 공통 기준 개발에 관한 연구)

  • Lee, Young-Han;Kim, Jeong-Dong;Park, Jeong-Ho
    • Journal of Internet Computing and Services
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    • v.17 no.3
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    • pp.75-85
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    • 2016
  • Changes in the distribution and sharing information using the Internet is changing the most part in our daily life, Utilizing the Internet of our lives to the rapid development of IT technology, are living in the off-line (off-line) and online (on-line) a mix of life, We expect now online life specific gravity is further increased. However, do not systematically implement the education of Internet ethics awareness deficiencies of objective indicators to assess the current knowledge level of the Internet ethics is the actual situation. Therefore, in this paper, it provides a development process in order to establish a common standard for the evaluation of Internet ethics index. Development procedure of Internet ethics index in order to establish a common standard, "evaluation item (draft) derivation", "Creating a questionnaire", "the calculation of the analysis and item-by-item weight of the questionnaire", and a common reference determination of the stage having. Was also a questionnaire that target 46 people to calculate the weight of the common reference. Internet ethics index evaluation model through the establishment of the common standards developed in this paper, municipal road Office of Education, related organizations, high school, such as small, medium, can be utilized as an indicator to measure the Internet ethics education level.

Daily Water Intake and Exposure Parameters Related to the Multi-route Exposure in Drinking Water (음용수의 섭취량 및 다경로 노출평가를 위한 노출변수 조사연구)

  • Chung, Yong;Shin, Dong-Chun;Park, Seong-Eun;Choi, Shi-Nai;Park, Seon-Mee
    • Environmental Analysis Health and Toxicology
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    • v.11 no.1_2
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    • pp.19-29
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    • 1996
  • Human exposure to volatile compounds in tap water can occur from inhalation and dermal absorption as well as direct ingestion. The relative contribution to total human exposure from these pathways has been considered to be important especially for VOC's (volatile organic compounds). In an attempt to reduce the uncertainty of the risk assessment, it has been suggested that the exposure assessment process could be significantly improved by adopting Monte-Carlo simulation. However, there is no actual data in Korea for each exposure parameter to determine the level of exposure, and the distributional pattern. Therefore, we surveyed water use patterns and behavior related to multi-route exposure to VOC's in household tap water in Korea, and compared these values to the those in western countries. In the first survey, we calculated daily water intake using data from a sample of 1322 persons of several cities in Korea. In the second survey, we obtained questionnaire data on exposure time for showering, bathing and household activities, and tap water intake from 851households in Korea. In the last survey, we measured the exposure parameters (exposure time, water use rate etc.) related to showers, baths, toilets, dish washing, washing and cooking, and tap water intake was surveyed. Also, the subjects were measured their body weight, height and tidal volume, etc. A diary, a flow meter and a measuring cup were used to measure these values as precisely as possible. Average daily water intake was ranged 0.79-1.71 L/day for adults in three surveys. Tap water intake measured by log-sheet during one week in third survey was 1.26 (average), 1.98 L/day (90 percentlie), respectively. These results were comparable with results from EPA (1.4, 2L/day). The average amount of water used by housewives in the third survey was 515.0 $\pm$ 564.6L/day. In usual activity, the amount of water used in the bathroom, the laundry and the kitchen was 140.0 $\pm$ 538.9, 148.0 $\pm$ 174.5, 229.3 $\pm$ 205.4 L/day, respectively. Exposure parameters such as water intake rate, exposure duration, body weight, inhalation rates in surveyed data of Korean people differed from those published from western countries. This could be attributed to variations in lifestyle, dietary habits and physiological characteristics.

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Assessment of Physical Activity, Activity Coefficient of Preschool Children and Actual Condition of Daycare Center Outdoor Play (미취학아동의 1일 활동량, 활동계수 평가 및 유아보육시설의 실외놀이 실태)

  • Kim, Jae-Hee;Kim, Eun-Kyung
    • Korean Journal of Community Nutrition
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    • v.14 no.6
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    • pp.777-788
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    • 2009
  • The purpose of this study was to assess physical activities and activity coefficient of preschool children and was to give some concrete information to activate outdoor play and to probe the suggestions to activate outdoor play. 42 preschool children (17 boys and 25 girls) were included. Body weight and height were measured and one-day activity diaries were collected by interviewing with their parents and kindergarten teachers. To measure physical activity during outdoor play, this study was researched the current conditions and content of outdoor play activity in daycare centers, and teacher's perceptions of that outdoor play. The average age of the subjects was 5.0 years. Average height, weight, body fat and body muscle of subjects were 108.9 cm, 18.9 kg, 17.7% and 26.8%, respectively. The subjects spent about 10 hours 39 minutes sleeping; 4 hours 16 minutes personal hygiene and computer working. They spent 88.2% of 24 hours (one day) in "very light activities" and 11.3% in "light activities". Activity coefficient (1.34) of weekday was significantly higher than that of weekend (1.21). And in this study, it was found that most of daycare centers (87.7%) have outdoor play space, 95.2% of them were garden. The teachers recognized that have a outdoor play frequency everyday (48%), three or four times (46%) in a week but they have three or four times (38%), one or two times (30%) because of various indoor program. Also, they recognized that have a outdoor play expending time 21-30 minutes (48%), 31-40 minutes (26%) but they have 21-30 minutes (64%). They answered that have frequency and expending time less than their recognition of outdoor play. Most of daycare center teachers perceived outdoor play to be as important as indoor play, and the teachers believed outdoor play supports children's physical, cognitive, social, and language development. The results of this study may be used to utilize as a basic data for estimate physical activity for preschool children and developed that exercise program to increase physical activity of daycare center outdoor play.