• Journal of Life Science
• /
• v.26 no.10
• /
• pp.1182-1188
• /
• 2016

Membrane-associated guanylate kinase inverted-3 (MAGI-3) is a member of the family of membrane-associated guanylate kinases (MAGUKs). MAGI-3 modulates the kinase activity of protein kinase B (PKB)/AKT through interactions with phosphatase and tensin homolog (PTEN)/MMAC. Coxsackievirus B3 (CVB3) is a common causative agent of acute myocarditis and chronic dilated cardiomyopathy. Activation of AKT and extracellular signal-regulated kinases 1/2 (ERK1/2) is essential for CVB3 replication, but the relation between MAGI-3 signaling and CVB3 replication is not well understood. This study investigated the role of MAGI-3 in CVB3 infection and replication. MAGI-3 was overexpressed in HeLa cells by polyethylenimine (PEI) transfection. To optimize the transfection conditions, different ratios of plasmid DNA to PEI concentrations were used. MAGI-3 and empty plasmid DNA were transfected into the HeLa cells. MAGI-3 overexpression alone was not sufficient to efficiently activate AKT. However, expression of the CVB3 capsid protein VP1 dramatically increased in the HeLa cells overexpressing MAGI-3 24 h after CVB3 infection. In addition, the activities of AKT and ERK were significantly induced in the CVB3-infected MAGI-3 cells overexpressing HeLa. These results demonstrate that MAGI-3 expression upregulates CVB3 replication through AKT and ERK signaling activation. MAGI-3 may be an important target to control CVB3 replication.

• Molecules and Cells
• /
• v.20 no.3
• /
• pp.305-314
• /
• 2005

Investigation of chemically synthesized inositol 1,4,5-trisphosphate [$Ins(1,4,5)P_3$] analogs has led to the isolation of a novel binding protein with a molecular size of 130 kDa, characterized as a molecule with similar domain organization to phospholipase C-${\delta}1$ (PLC-${\delta}1$) but lacking the enzymatic activity. An isoform of the molecule was subsequently identified, and these molecules have been named PRIP (PLC-related, but catalytically inactive protein), with the two isoforms named PRIP-1 and -2. Regarding its ability to bind $Ins(1,4,5)P_3$ via the pleckstrin homology domain, the involvement of PRIP-1 in $Ins(1,4,5)P_3$-mediated $Ca^{2+}$ signaling was examined using COS-1 cells overexpressing PRIP-1 and cultured neurons prepared from PRIP-1 knock-out mice. Yeast two hybrid screening of a brain cDNA library using a unique N-terminus as bait identified GABARAP ($GABA_A$ receptor associated protein) and PP1 (protein phosphatase 1), which led us to examine the possible involvement of PRIP in $GABA_A$ receptor signaling. For this purpose PRIP knock-out mice were analyzed for $GABA_A$ receptor function in relation to the action of benzodiazepines from the electrophysiological and behavioral aspects. During the course of these experiments we found that PRIP also binds to the b-subunit of $GABA_A$ receptors and PP2A (protein phosphtase 2A). Here, we summarize how PRIP is involved in $Ins(1,4,5)P_3$-mediated $Ca^{2+}$ signaling and $GABA_A$ receptor signaling based on the characteristics of binding molecules.

• The Journal of Korean Medicine
• /
• v.19 no.2
• /
• pp.296-312
• /
• 1998

BACKGROUND The purpose of this study was to find out predictors influencing on the clinical course of stroke during the first 4 weeks after the onset through statistical research, especially whether the blood glucose level after stroke and the stroke with Diabetes Mellitus correlate with functional impairments and neurological outcome or not. METHOD During 7 months period(from 1-1-1997 to 7-31-1997), 32 selected patients prognosed as acute cerebral thrombosis were studied by using Modified Barthel Index, PULSES profile in an attempt to correlate Diabetes Mellitus and hyperglycemia(more than $120mg/d{\ell}$, $150mg/d{\ell}$) with functional impairment and neurological outcome and to evaluate the influence of sex, the side of hemiparesis and age at admission, 1 week and 4 weeks after admision(admitted within 2 days after the onset). RESULT 1. The sex, side of hemiparesis and age had no significant effect upon functional impairment during first 4 weeks after the onset, but recurrent-stroke resulted in significantly higher degree of functional impairment than first-stroke during first 4 weeks after the onset. 2. The patients with Diabetes Mellitusin in acute cerebral thrombosis resulted in significantly higher degree of functional impairment than the patients without Diabetes Mellitus in acute cerebral thrombosis during first 4 weeks after the onset. 3. The patients with hyperglycemia in acute cerebral thrombosis resulted in significantly more severe neurological outcome than the patients without hyperglycemia in acute cerebral thrombosis within 2 days after the onset. CONCLUSION The study suggested that recurrent-stroke and Diabetes Mellitus were the poorer prognosis factors of functional impairment in acute cerebral thrombosis patients during first 4 weeks after the onset. and the poorer prognosis factor of neurological outcome in acute cerebral thrombosis patients was hyperglycemia within 2 days after the onset.

• Journal of Astronomy and Space Sciences
• /
• v.26 no.1
• /
• pp.9-24
• /
• 2009

We compare the relation among the annual distribution of sunspots: coronal mass ejections (CMEs) and geomagnetic storms and North-South asymmetry during solar cycle 23. For this purpose, we calculate correlation coefficients between (i) annual distribution and N-S asymmetry of CMEs - sunspots (ii) distribution of CMEs - occurrence number of geomagnetic storms (iii) distribution of sunspots - occurrence number of geomagnetic storms. We find that (i) the annual distribution of total CMEs has good correlation with distribution of annual average of sunspots but poor correlation with N-S asymmetry of sunspots, N-S asymmetry of CMEs has good correlation with N-S asymmetry of sunspots: (ii) total and N-S asymmetry of CMEs have poor correlation with occurrence number of geomagnetic storms, it's, however, well correlated with the classified groups of CMEs (Ap, Dst and an indices vs. fast CMEs($\upsilon$ > $1000kms^{-1}$), Dst index vs. Halo CMEs), and (iii) sunspot numbers and area are correlated with occurrence number of geomagnetic storms. We conclude that annual distribution of CMEs and sunspots have well correlated with geomagnetic storms, N-S asymmetry of CMEs and sunspots have poor correlated with the geomagnetic storms.

• Journal of Distribution Science
• /
• v.11 no.1
• /
• pp.35-44
• /
• 2013

Purpose - This study aims to (a) analyze local citizens' perception of a local company, the Gwangyang Steel and Iron Company (GSIC); (b) compare the perception toward the local company with that of national citizens' perceptions towards many other unspecified companies, which were surveyed biannually by The Korea Chamber of Commerce and Industry (KCCI); and (c) analyze how such companies evaluate perceptions towards them, in relation to citizens' socioeconomic position, such as their education status. Research design, data, methodology - The data were collected using a questionnaire, which was given to a sample of local citizens in cities. To test the hypotheses, factor analyses, a t-test, and an ANOVA were conducted. The total number of respondents was 1798. The data gathered from the respondents of the sample were analyzed using SPSS Win 19.0 software. Perception towards the company was evaluated on the corporate favorite index (CFI). The CFI index shows how positive citizens feel towards a company. It is calculated using five leading factors, consisting of the following: contribution to the economy, productivity, international competitiveness, social contribution, and ethical management. The higher the level of positive feelings exhibited, the closer the index will come to 100. Results - As a result of the analysis, the CFI index towards the GSIC stood at 67.3. The CFI index towards the company is 16.5 points higher than that of the index towards the many other unspecified companies, which were surveyed by the bi-annual KCCI study in the first half of 2011. The other five indexes stood as follows: contribution to the economy, productivity, social contribution, and ethical management was 69.2, 71.2, 64.6, and 58.6, respectively. These indexes are 18.3, 4.6, 27.6, and 35.6, respectively, higher than those found by the KCCI study. However, international competitiveness is 73.0 (9.8 points lower than that of the KCCI study). This survey thus shows that the CFI of the local citizens towards the global company GSIC is very high when compared with the CFI index towards the many other unspecified companies that was determined by the KCCI survey. Conclusions - This survey shows that local citizens have high expectations of economic activity, increasing working opportunities, and regional cooperation projects from the local company, GSIC. In addition, the CFI index towards GSIC evaluates results depending on the respondent's relationship with the company, and their educational status. Respondents, whose family or relatives were working as employees of GSIC, have relatively positive perceptions of GSIC, and respondents with a relatively higher educational status also share positive perceptions. Local citizens expect the profit-making of the company to operate in accordance with management activities, and at the same time, they expect that the wealth generated by the company will return to wider society.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.27 no.4
• /
• pp.517-523
• /
• 2000

Nasopharyngeal closure is a sphincter mechanism between the activities of the soft palate, lateral pharyngeal wall and the posterior pharyngeal wall, which divides the oral cavity and the nasal cavity. It participates in physiological activities such as swallowing, breathing and pronunciation. In case of an error in this mechanism, it is called a nasopharyngeal incompetence. The causes of this error are defects in (1) length, function, posture of the soft palate (2) depth and width of the nasopharynx, (3) activity of the posterior and lateral pharyngeal wall. The purpose of this study is to analyze the nasopharynx of cleft palate patients using lateral cephalograms and at the same time, evaluate the degree of hypernasality of each vowels to find its relationship with nasopharyngeal incompetence. The following results were obtained: 1. The length of the soft palate was markedly short than normal. 2. The adequate ratio was smaller than the normal value. 3. As the adequate ratio decreased, when articulating vowels, anatomic mVPI increased. 4. When articulating each vowels, anatomic VPI was in proportion with the degree of hypernasality. 5. The degree of hypernasality was greater in high vowels(/i/, /u/) than low vowel(/a/). From the above results, it can be concluded that in cleft palate patients, lateral cephalograms can be used effectively in diagnosing and evaluating nasopharyngeal incompetence. The anatomic structure of the nasopharynx has close relation to the degree of hypernasality.

• Sleep Medicine and Psychophysiology
• /
• v.21 no.1
• /
• pp.29-32
• /
• 2014

Objectives: The aim of current study is to evaluate the relationship between sleep, suicide and serotonin using some scales and loudness dependence of auditory evoked potentials (LDAEP). Methods: Total 65 patients who met the criteria for major depressive disorder were enrolled in current study. The patients were divided into two subgroups according to their insomnia and a history of suicide attempts. The auditory event-related potentials were measured to evaluate LDAEP before beginning antidepressants. Results: The scores of total Beck Depression Inventory (BDI) and BDI item 9 (suicide) were higher in insomnia subgroup than non-insomnia subgroup (respectively, p=0.0033 and p=0.03). However, LDAEP did not differ each other. The subgroup with a history of suicide attempts had a higher score of BDI item 9 than the subgroup without a history of suicide attempts (p=0.00012). There was a tendency for the LDAEP to be higher in the subgroup with a history of suicide attempts ($1.39{\pm}0.94{\mu}V$) than the sub-group without a history of suicide attempts ($1.05{\pm}0.75{\mu}V$), although the difference was not statistically significant (p=0.078). Conclusion: Suicidality was related to insomnia. In addition, there was a tendency for serotonin activity to be lower in the subgroup with a history of suicide attempts. In future, more studies are needed.

• Physical Therapy Korea
• /
• v.8 no.2
• /
• pp.41-54
• /
• 2001

Fatigue is the decline in force produced as a result of prolonged muscle activity. Localized muscle fatigue can be identified by a shift toward low in the frequency components of the EMG signal, typically represented by a fall in the median frequency. Previous studies show that a shortened muscle develops a higher fatigue than elongated muscles. The purpose of this study was to investigate the time-related change of median frequency and torque during maximal isometric back extension exercises at different exercise angles ($0^{\circ}$, $12^{\circ}$, $36^{\circ}$, $72^{\circ}$). Twenty healthy subjects (mean age = $24.35{\pm}2.70$) were evaluated in this study. Median frequency was extracted from the EMG signals by fast Fourier transform (FFT). Initial median frequency and the slope of median frequency change over time were computed from linear regression analysis. Pearson's product moment correlation was used to quantify the relationship between slope of median frequency and torque. The results were as follows: 1) Significant differences in y-intercepts of torque regression equation with respect to exercise angle were shown. However, there were no differences in the slopes of the median frequency and torque, and y intercept of the median frequency among exercise angles. 2) There was no significant correlation between slope of median frequency and torque. 3) But there was moderate correlation between median frequency and torque at each exercise angle. In conclusion, the exercise angle during maximal isometric back extension exercise is not a direct effect on slope of median frequency and torque. But results showed that median frequency and torque shift were highly correlated in all subjects.

• Journal of the Korean Chemical Society
• /
• v.54 no.5
• /
• pp.515-522
• /
• 2010

A relation between antimicrobial activities and the formation constants of solid complexes of Cu(II), Ni(II), Co(II), Mn(II) and Fe(III) with tridentate Schiff base ligand, 4-hydroxy-3(1-{2-(benzylideneamino)-phenylimino}-ethyl)-6-methyl-2Hpyran-2-one (HL) derived from o-phenylene diamines, dehydroacetic acid (DHA) and p-chloro benzaldehyde have been studied. The ligand and metal complexes were characterized by elemental analysis, conductivity, magnetic susceptibility, thermal analysis, X-ray diffraction, IR, $^1H$-NMR, UV-vis and mass spectra. From the analytical data, the stiochiometry of the complexes was found to be 1:2 (metal:ligand) with octahedral geometry. The molar conductance values suggest the nonelectrolytic nature of metal complexes. The X-ray diffraction data suggests monoclinic crystal system for Ni(II) and orthorhombic crystal system for Cu(II) and Co(II) complexes. The IR spectral data suggest that the ligand behaves as tridentate ligand with ONN donor atoms sequence towards central metal ion. Thermal behavior (TG/DTA) and kinetic parameters calculated by Coats-Redfern method suggests more ordered activated state in complex formation. The protonation constants of the complexes were determined potentiometrically in THF:water (60:40) medium at $25^{\circ}C$ and ionic strength ${\mu}=0.1\;M$ ($NaClO_4$). Antibacterial activities in vitro were performed against Staphylococcus aureu and Escherichia coli. Antifungal activities were studied against Aspergillus Niger and Trichoderma. The effect of the metal ions and stabilities of complexes on antimicrobial activities are discussed.

• Child Health Nursing Research
• /
• v.6 no.1
• /
• pp.32-50
• /
• 2000

A descriptive exploratory design was used in this study to evaluate the effects of sponge bathing on physiological(heart rate, heart period, vagal tone, oxygen saturation, respiration) and behavioral responses in newly born 40 preterm infants from intensive care unit of S University Hospital in Seoul. Data has been collected from October, 1997 to March, 1999. The infants were between 27-33 weeks gestational age at birth, and were free of congenital defects. The subjects entered the protocol when they were medically stable (determined by initiation of feeding and discontinuation of all respiratory support) but still receiving neonatal intensive care. The infants' physiologic parameters were recorded a 10 - minute before, during, and after bathing. Continuous heart rate data were recorded on a notebook computer from the infant's EKG monitor. The data were digitized off-line on software(developed by Lee and Chang in Wavelet program) which detected the peak of the R wave for each heart beat and quantified sequential R-R intervals in msec(i.e. heart periods). Heart period data were edited to remove movement artifact. Heart period data were quantified as : 1) mean heart period; 2) vagal tone. Vagal tone was quantitfied with a noninvasive measure developed by Porges(1985) in Mxedit software. To determine behavioral status, tools were developed by Scafidi et al(1990) were used. Collected data were analyzed with the SPSS program using paried t-test, ANOVA, and Pearson correlation. The result were as follow. 1. The results of the ANOVAs indicated that vagal tone were signifcantly lower during bathing than baseline and post-bathing. There were significant differences in heart period and heart rate levels across the bathing. But the mean oxygen saturations and respirations were no differences. Also, there were no significant differences on behavioral sign, motor activity, behavioral distress, weight changes, morbidity, and hospitalization period. 2. To evaluate the relation between vagal tone and subsequent parameters, the two groups (the high group had 19 subjects and low group had 21subjects) were divided by the mean baseline vagal tone. Vagal tone measured prior to bathing were significantly associated with respiration before bathing, vagal tone during bathing, and the magnitude of change in both vagal tone. But, other subsequent reactivities were no differences in two groups. 3. Correlations were also calculated between vagal tone and the subsequent physiological reactivities from baseline through after- bathing. Correlations were significant between baseline vagal tone and baseline heart rate, between baseline vagal tone and baseline heart period, between baseline vagal tone and oxygen saturation after bathing. In summary, the bathing in this study showed a stressful stimulus on premature infants through there was significance in the physiological parameters. In addition, our study represents the documentation that vagal tone reactivity in response to clearly defined external stimulation provides an index of infant's status.