• Title/Summary/Keyword: activity energy

Search Result 2,884, Processing Time 0.04 seconds

Purification of Hydrogenase from Thiocapsa roseopersicina : Effect of Ammonium Sulfate Precipitation and Heat-Treatment (홍색 유황세균 Thiocapsa roseopersicina가 생산하는 수소생산 효소의 정제 : Ammonium sulfate 침전 및 열처리의 효과)

  • Choi, Eun-Hye;Oh, You-Kwan;Kim, Mi-Sun
    • Journal of Hydrogen and New Energy
    • /
    • v.17 no.4
    • /
    • pp.371-378
    • /
    • 2006
  • Effect of $(NH_4)_2SO_4$ precipitation and heat-treatment on hydrogenase which was extracted from the cytoplasmic fraction of the phototrophic purple sulfur bacterium Thiocapsa roseopersicina NCIB 8347 was studied. Crude enzyme extract was prepared by centrifugation($28,000{\times}g$, $400,000{\times}g$) after sonication of cells grown under photosynthetic condition for 96 hrs. Various conditions of $(NH_4)_2SO_4$ precipitation and heat-treatment were examined and the effect of protein concentration was analyzed by SDS-electrophoresis between the treatments. Optimum conditions for $(NH_4)_2SO_4$ precipitation and heat-treatment for evolution hydrogenase activity were 40-60% saturation and $60^{\circ}C$ for 20 min, respectively, which exhibited the specific hydrogenase activity of 0.78 U/mg-protein. Specific hydrogenase activity was decreased to 31.6% when the heat-treatment at $60^{\circ}C$ increased from 20 min to 5 hrs.

Development of Anticosmic Shielded Ultra Low Background Gamma Spectrometer for Precise Measurement of Environmental Radioactivity

  • Byun, Jong-In;Park, Yun-Ho;Kwak, Seung-Im;Hwang, Han-Yull;Chung, Kun-Ho;Park, Gun-Sik;Park, Doo-Won;Lee, Chnag-Woo
    • Nuclear Engineering and Technology
    • /
    • v.34 no.6
    • /
    • pp.545-552
    • /
    • 2002
  • We developed an ultra low background gamma ray spectrometer particularly suitable for experiment which require lower detection limit. The background of a germanium spectrometer is suppressed by applying active and passive shielding technique at the same time. The active shielding devices consist of plastic scintillating plates of 50 mm thick and anti-coincidence electronic system. The shielding is made of 150 mm thick walls of very low activity lead,20 mm with activity of <10 Bq/kg and 130 mm with activity of <50 Bq/kg. The observed background count rates are 1.2 $s^{-1}$ and 0.36 $s^{-1}$ without and with the active shielding, respectively, overall the energy regions from 30 keV to 3 MeV The cosmic ray induced background is suppressed by a rate of 0.8 $s^{-1}$ at the present work. The detection efficiency curve necessary to obtain the radioactivity of environmental samples has been precisely determined on the energy regions from 80 to 2000 keV with a 10$^3$ ml marinelli beaker sample, consisting of the calibrated radionuclides $^{109}$ Cd, $^{57}$ CO, $^{139}$ Ce, $^{203}$ Hg, $^{113}$ Sn, $^{85}$Sr, $^{137}$ Cs, $^{60}$ Co and $^{88}$ Y. Virtues Of the method are demonstrated by measuring the activity of $^{137}$ Cs contained in the powdered milk.

Reconstruction Effect of the Spectral Entropy for the Voice Activity Detection (음성 활동 구간 검출을 위한 스펙트랄 엔트로피의 재구성 효과)

  • Kwon HO-Min;Han Hag-Yong;Lee Kwang-Seok;Koh Si-Young;Hur Kang-In
    • Proceedings of the Acoustical Society of Korea Conference
    • /
    • spring
    • /
    • pp.25-28
    • /
    • 2002
  • Voice activity detection is important Problem in the speech recognition and communication. This paper introduces feature parameter which is reconstructed by the spectral entropy of information theory for the robust voice activity detection in the noise environment, analyzes and compares it with the energy method of voice activity detection and performance. In experiment, we confirmed that the spectral entropy is more feature parameter than the energy method for the robust voice activity detection in the various noise environment.

  • PDF

Effect of Support Geometry on Catalytic Activity of Pt/CeO2 Nanorods in Water Gas Shift Reaction (Water Gas Shift 반응에서 Pt/CeO2 촉매의 지지체구조에 따른 촉매활성 연구)

  • Im, Hyo Been;Kwon, Soon Jin;Byun, Chang Ki;Ahn, Hee Sung;Koo, Kee Young;Yoon, Wang Lai;Yi, Kwang Bok
    • Journal of Hydrogen and New Energy
    • /
    • v.25 no.6
    • /
    • pp.577-585
    • /
    • 2014
  • Nanorod and particle shape $CeO_2$ were synthesized via hydrothermal process and precipitation method, respectively, and used as supports of Pt catalyst for water gas shift (WGS) reaction. Three different durations (12, 48, and 96h) for hydrothermal process were applied for the preparation of nanorod type $CeO_2$. 1.0 wt% of Pt was loaded on the prepared supports with incipient wetness method prior to the catalytic activity tests that were carried out at a GHSV of $95,541h^{-1}$, and a temperature range of 200 to $360^{\circ}C$. Varying duration of hydrothermal process led to the difference in physical characteristics of $CeO_2$ nanorods, such as aspect ratio, BET surface area, pore diameter, and pore volume. Consequently, the catalytic activities of Pt/$CeO_2$ nanorods were affected by the physical characteristics of the supports and appeared to be in the order of Pt/$CeO_2$(12) > Pt/$CeO_2$(48) > Pt/$CeO_2$(96). The comparison of the catalytic activities and results of the analysis (XPS, XRD, SEM, BET and TPR) for the supports revealed that the activity of the catalysts depends on chemical states of the Pt and the support materials in the temperature range that is lower than $280^{\circ}C$. However, the activity is rather dependent on the physical characteristic of the supports because the increased gas velocity limits the mass transfer of reactants in micropores of the supports.

Establishment and future tasks of estimated energy requirement in 2020 dietary reference intakes for Koreans (2020 한국인 에너지필요추정량 설정 및 앞으로의 과제)

  • Kim, Eun-Kyung;Kim, Oh Yoen;Park, Jonghoon;Kim, EunMi;Kim, Juhyeon
    • Journal of Nutrition and Health
    • /
    • v.54 no.6
    • /
    • pp.573-583
    • /
    • 2021
  • Energy requirement is defined as energy expenditure in an energy equilibrium state. The doubly labeled water (DLW) method is considered the gold standard for measuring total energy expenditure (TEE). In 2002, the Institute of Medicine (IOM) of the National Academies established dietary reference intakes (DRIs) for Americans and Canadians, and the equations for estimated energy requirement (EER) were developed by using pooled data from studies that had applied the DLW method. Since 2005, these equations have been used for establishing EER in the DRI for Koreans. These equations based on age group include the physical activity (PA) coefficient determined by the PA level (PAL; sedentary, low active, active and very active) as well as body weight and height. The PAL values of Koreans calculated using the DLW method and PA diaries were determined to fall in the low active category (1.40~1.59). Therefore, the PA coefficient corresponding to 'low active' was applied to the EER equations. In recent years, with increasing number of people regularly engaging in various physical activities in Korea, EER is now separately presented for people with 'active' and 'very active' PALs. In the future, like the United States and Japan, Korea needs to expand the DLW research for developing EER predictive equations for Koreans. In addition, standardized guidelines should be prepared to accurately evaluate the PAL using the physical activity diary and the new PA classification table for Koreans.

Investigation on the Increase of Antioxidant Activity of Cooking Drip from Enteroctopus dofleini by Irradiation (방사선 조사에 의한 문어 자숙액의 항산화 활성 증가 원인 규명)

  • Choi, Jong-Il;Kim, Yeon-Joo;Sung, Nak-Yun;Kim, Jae-Hun;Ahn, Dong-Huyn;Chun, Byung-Soo;Cho, Kook-Yeon;Byun, Myung-Woo;Lee, Ju-Woon
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.38 no.1
    • /
    • pp.121-124
    • /
    • 2009
  • This study was conducted to investigate the reason for the increase in the antioxidant activity of cooking drip from Enteroctopus dofleini by gamma and electron-beam irradiation. To define the effect of irradiation on the antioxidant activity of polyphenol in cooking drip, polyphenol excluded cooking drip extracts were prepared with polyclar$^{TM}$. But, the antioxidant activity of the cooking drip extracts without polyphenol was still increased by both irradiations. Instead, the effect on the proteins in the cooking drips prepared by ammonium sulfate precipitation showed great increase in antioxidative activity by irradiation. From these results, it was concluded that the increase in the antioxidative activity in cooking drips by the irradiation was caused by the structural modification of proteins in the cooking drips.

The Transport Characteristics of 238U, 232Th, 226Ra, and 40K in the Production Cycle of Phosphate Rock

  • Jung, Yoonhee;Lim, Jong-Myoung;Ji, Young-Yong;Chung, Kun Ho;Kang, Mun Ja
    • Journal of Radiation Protection and Research
    • /
    • v.42 no.1
    • /
    • pp.33-41
    • /
    • 2017
  • Background: Phosphate rock and its by-product are widely used in various industries to produce phosphoric acid, gypsum, gypsum board, and fertilizer. Owing to its high level of natural radioactive nuclides (e.g., $^{238}U$ and $^{226}Ra$), the radiological safety of workers who work with phosphate rock should be systematically managed. In this study, $^{238}U$, $^{232}Th$, $^{226}Ra$, and $^{40}K$ levels were measured to analyze the transport characteristics of these radionuclides in the production cycle of phosphate rock. Materials and Methods: Energy dispersive X-ray fluorescence and gamma spectrometry were used to determine the activity of $^{238}U$, $^{232}Th$, $^{226}Ra$, and $^{40}K$. To evaluate the extent of secular disequilibrium, the analytical results were compared using statistical methods. Finally, the distribution of radioactivity across different stages of the phosphate rock production cycle was evaluated. Results and Discussion: The concentration ratios of $^{226}Ra$ and $^{238}U$ in phosphate rock were close to 1.0, while those found in gypsum and fertilizer were extremely different, reflecting disequilibrium after the chemical reaction process. The nuclide with the highest activity level in the production cycle of phosphate rock was $^{40}K$, and the median $^{40}K$ activity was $8.972Bq{\cdot}g^{-1}$ and $1.496Bq{\cdot}g^{-1}$, respectively. For the $^{238}U$ series, the activity of $^{238}U$ and $^{226}Ra$ was greatest in phosphate rock, and the distribution of activity values clearly showed the transport characteristics of the radionuclides, both for the byproducts of the decay sequences and for their final products. Conclusion: Although the activity of $^{40}K$ in k-related fertilizer was relatively high, it made a relatively low contribution to the total radiological effect. However, the activity levels of $^{226}Ra$ and $^{238}U$ in phosphate rock were found to be relatively high, near the upper end of the acceptable limits. Therefore, it is necessary to systematically manage the radiological safety of workers engaged in phosphate rock processing.