• Journal of Plant Biotechnology
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• 제3권1호
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• pp.1-5
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• 2001

Traditional culture technology of medicinal plants mainly depends on the field culture, which has many problems. With progress of modern culture technology, it has become possible to produce valuable secondary metabolites from medicinal plants. In this paper, we discuss about the pigment and saikosaponin production from too medicinal plants, Carthamus tinctorius and Bupleurum falcatum, through bioreactor culture system. A two-stage bioreactor culture system was established for the production of yellow and red pigments and saikosaponins by cell suspension cultures of Carthamus tinctorius and Bupleurum falcatum. In Carthamus tinctorius, balloon type airlift bioreactors and column type airlift bioreactors were employed for the tell culture and for the pigment production, respectively. The greatest pigment production was obtained on White medium supplemented with 4 mg/L kinetin, high levels of sucrose concentration and photosynthetic photon flux. In Bupleurum falcatum, adventitious roots were cultured in balloon type airlift bioreactors and the root growth was greatest on SH medium containing 5 mg/L IBA and 0.2 mg/L kinetin. HPLC analysis showed that the contents of main active saikosaponins a, c, and d in adventitious roots were almost the same as those in field cultured root.

• KSBB Journal
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• 제8권5호
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• pp.473-477
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• 1993

하이브리도마 배양을 위해 첨가한 기존의 혈 청농도는 10% FBS였으나, 이 농도는 3%까지 줄여도 세포의 비성장속도와 최고세포농도에 차이가 없었다. 세포성장을 촉진한다고 알려진 물 질들을 1% FBS 배지에 첨가하였을 때 insulin, pyruvate, oxaloacetate, pluronic F-68 등에 의해 세포성장이 촉진되었다. 3% FBS는 2% CS로 대치할 수 있었고, 혈청의 농도는 IPOP의 첨가시 0.5 % CS에서도 active proliferation을 보였다. 최종 조성의 배지가격은 기존의 10 % FBS 배지의 1/10 정도였으며 배지조성을 cell line에 따라 달리 결정함으로써 배지의 가격은 현저히 낮출 수 있음을 알았다.

• The Korean Journal of Physiology
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• 제10권1호
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• pp.7-14
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• 1976

The Present study was conducted to investigate the effects of Ginseng extract on the tension-area curve for stearic acid monolayer. At the same time, the effects of Ginseng extract on osmotic and mechanical fragility of human red cells and histamine release from rabbit leukocytes were studied, The results are summarized as follows. 1. The Ginseng alcohol extract was found to expand liquid expanded phase of stearic acid monolayer, thus it is speculated that this agent may be acting as a surface active substance. 2. Osmotic hemolysis was inhibited by the Ginseng alcohol extract and the same effect was also observed in the presence of Ginseng saponin. However, the Ginseng alcohol extract was found to decrease hematocrit ratio of the RBC suspension, therefore, the inhibition of the osmotic hemolysis by this agent may be secondary effect to the reduced cell volume. 3. The mechanical hemolysis was also inhibited by the Ginseng alcohol extract but the inhibition was independent of changes in hematocrit ratio. 4. Histamine release from rabbit leukocytes was significantly increased in vitro in the presence of the Ginseng alcohol extract.(p<0.05)

• 한국소음진동공학회논문집
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• 제18권3호
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• pp.299-306
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• 2008

The electromagnet in Maglev vehicles controls the voltage in its winding to maintain the air gap, a clearance between the electromagnet and guideway, within an allowable deviation, with strongly interacting with the flexible guideway. Thus, the vibration characteristics of guideway plays important role in dynamics of Maglev vehicles using electromagnet as an active suspension system. The effects of the guideway's vibrational characteristics on dynamics of the Maglev vehicle UTM-01 are analyzed. The coupled equations of motion of the vehicle/guideway with 3 DOFs are derived. Eigenvalues are calculated and frequency response analysis is also performed for a clear understanding of the dynamic characteristics due to guideway vibration characteristics. To verify the results, tests of the urban Mgalev vehicle UTM-02 are carried out. It is recommended that the natural frequency of the guideway be minimized and its damping ratio in the Maglev vehicle with a 5-states feedback control law as a levitation control law.

• International Journal of Industrial Entomology and Biomaterials
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• 제17권2호
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• pp.181-187
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• 2008

An entomopathogenic fungus, Paecilomyces fumosoroceus SFP-198 was isolated in Kyungbuk province, Korea and screened out for the control of Trialeurodes vaporariorum nymphs. It showed 72.5% efficacy against second instars of T. vaporariorum nymphs at 6 days after treatment in the laboratory. To select an active ingredient for the mass production and the formulation, SFP-198 culture products were compared based on their insecticidal activities against T. vaporariorum nymphs. Among them, conidia and blastospores showed much higher insecticidal activity than supernatants. Furthermore, SFP-198 conidia were more heat-resistant than blastospores, and also powder form of conidia was more stable than their suspension form. SFP-198 conidia showed high pathogenicity on not only T. vaporariorum but also Tetranychus urticae in the glasshouse. This result suggested that SFP-198 conidia can be used as a multi-targeting biological control agent against sucking agri- cultural pests, such as whiteflies and mites.

• KSTLE International Journal
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• 제4권2호
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• pp.37-42
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• 2003

One of the obstacles for a magnetic bearing to be used in the wide range of industrial applications is the failure modes associated with magnetic bearings, which we don't expect for conventional passive bearings. These failure modes include electric power outage, power amplifier faults, position sensor faults, and the malfunction of controllers. Fault-tolerant magnetic bearing systems have been proposed so that the system can operate in spite of some faults in the system. In this paper, we designed a fault-tolerant magnetic bearing system for a turbo-molecular vacuum pump. The system can cope with the actuator/amplifier faults which are the most common faults in a magnetic bearing system. We implemented the existing fault-tolerant algorithms to experimentally prove the adequacy of the algorithms for industrial applications. As it turns out, the system can operate even with three simultaneously failing poles out of eight actuator poles.

• 한국정밀공학회지
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• 제25권3호
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• pp.73-82
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• 2008

Recently MR CDC damper has been applied to semi-active suspension control system gradually. Compared to former hydraulic CDC damper, it has rapid time response performance as well as simple internal structure and wide range of damping force. In order to develop control logic algorithm which enables to take maximum advantage of unique characteristics of MR CDC damper, it is inevitable to perform a thorough investigation into its nonlinear performance. In many previous researches, MR fluid model was either simply assumed as Bingham Plastic, or a phenomenological model based on experiment was established instead to predict damping performance of MR CDC damper. These experimental flow model which is not based on flow analysis but intentionally built to fit damping characteristics, may lead to totally different results in case of different configuration or structure of MR CDC damper. In this study, a generalized flow formula from mathematical flow model of MR fluid for annular orifice is derived to analyze and predict damping characteristics when current is excited at piston valve.

• 제어로봇시스템학회논문지
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• 제8권10호
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• pp.898-906
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• 2002

Antilock Brake System (ABS) is designed to prevent wheels from being locked-up under emergency braking of a vehicle. Therefore it improves directional stability of the vehicle, shortens stopping distance, and enhances maneuvering during braking, regardless of road conditions. Hardware In-the-Loop Simulation (HILS) is an effective tool for design Performance evaluation and test of vehicle subsystems such as ABS, active suspension, and steering systems. This paper describes a HILS model for ABS/ ASR(Acceleration Slip Regulation) system applications. A fourteen degrees-of-freedom vehicle dynamics model is simulated in an alpha-chip processor board. The proposed HILS system is tested with a basic ABS control algorithm. The design and implementation of HILS system for the ABS ECU(Electronic Control Unit) development of commercial vehicle are presented. The results show that the proposed HILS system can be used to test the performance, stability, and reliability of a vehicle under braking.

• 한국식품영양과학회지
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• 제32권7호
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• pp.1046-1052
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• 2003

Viscoelastic behavior of oxidized starch gel, modified with sodium hypochlorite (NaOCl) and the adding effects of protein in oxidized starch gel was studied by dynamic viscoelastic measurement. The storage modulus(G′) of starch gel increased with the increase of starch concentration. They showed higher value when starch suspension was treated to 95$^{\circ}C$ rather than 85$^{\circ}C$. Consistency of starch gel was decreased over 1.0% active Cl/g starch when heated to 95$^{\circ}C$, which means that the swelling of starch granules increased with concentration of NaOCl and showed more sensitive against shear. As the extent of oxidation increased, starch granules were easily destroyed. Therefore, it is hard to separate between compartment of leached-out amylose and that of amylopectin, which means that the ability of gel formation was reduced. When oxidized starches were gelatinized in presence of soy protein and sodium caseinate, it was found that G′ decreased, and frequency dependence of G′ and G" increased with the increased degree of oxidation in starch. The reduce of starch-protein interaction was thought to be through the dissociation of the branched amylopectin, which playa leading role in protein interaction, with the oxidation of starch.

• 한국자동차공학회논문집
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• 제16권3호
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• pp.1-6
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• 2008

The performance of shock absorber is directly related to the car behavior and performance, both for handling and comfort. Most of compact car are assembled the passive shock absorber for cost effect but some of compact driver want better performance of shock absorber than standard parts. Therefore, they want the semi-active suspension control system instead of standard damper system. But they only can change the mechanical damping control shock absorber at A/S market. The mechanical damping control shack absorber can not vary the damping force in driving condition so they do not satisfy the mechanical damping control shock absorber system. In this study, electrically damping force controlled shock absorber system is developed based on the mechanical damping force control damper system. This system can vary damping force by switch on dashboard in driving condition. And, this system can satisfy the requirement of tuning market. Therefore, it is expected the system to show the engineering capability of korean damper company and to increase export market share to oversea damper market.