• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.62-72
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• 2001

The N-carbamoyl-D-amino acid amidohydrolase (D-carbamoylase) gene (dcb) from Agrobacterium tumefaciens AM 10 has been successfully cloned and expressed in Escherichia coli. Expression of D-carbamoylase gene under the 17 promoter in different host strains showed that the optimal expression was achieved in E. coli JM109 (DE3) with a 9-fold increase in enzyme production compared to the wild-type strain. The co-expression of the GroEL/ES protein with D-carbamoylase protein caused an in vivo solubilization of D-carbamoylase in an active form. The synergistic effect of GroEL/ES at 28$^{\circ}C$ led to 60 ％ solubilization of the total expressed target protein with a 6.2-fold increase in enzyme activity in comparison to that expressed without GroEL/ES and 43-fold increase in enzyme activity compared to A. tumefaciens AM 10. Attempts to express D-carbamoylase in an altered redox cytoplasmic milieu did not improve the enzyme production in an active form. The Histidyl-tagged D-carbamoylase was purified in a single step by Nickel-affinity chromatography and was found to have a specific activity of 9.5 U/mg protein.

• Animal cells and systems
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• 제2권2호
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• pp.223-227
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• 1998

We isolated two soluble forms of glutamate dehydrogenase isoproteins, GDH I and GDH II, from bovine brain. The regulation of GDH I and GDH II by phosphorylation and dephosphorylation has been examined in various conditions. There were dose- and time- dependent activation of the GDH isoproteins when phosphorylated by cAMP-dependent protein kinase. The phosphorylated GDH had 1.1 mol of covalently bound phosphate/mol of subunit and a 2-fold increased specific activity. The phosphorylated amino acid was identified as serine. When treated with alkaline phosphatase, the activities of the phosphorylated GDH isoproteins were reduced in dose and time dependent manner and returned to those of unphosphorylated enzymes. There were no significant differences between GDH I and GDH II in their sensitivities to the action of phosphorylation and dephosphorylation demonstrating that the microenvironmental structures of the phosphorylation site in GDH isoproteins are similar to each other, These results results suggest that the inter-conversion between less active form of brain GDH isoproteins and more active form is regulated by phosphorylation through cAMP-dependent protein kineses.

• Journal of Pharmaceutical Investigation
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• 제27권3호
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• pp.219-223
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• 1997

Terfenadine, antihistaminic drug, is poorly soluble in water. The purpose of this study is to investigate the possibility of using $terfenadine-{\beta}-cyclodextrin$ inclusion compound, instead of terfenadine, as the active substance of solid dosage form by improving the solubility, dissolution and anti-histaminic activity of terfenadine. The solubility and binding characteristics of $terfenadine-{\beta}-cyclodextrin$ complex in pH $1.2{\sim}6.8$ were investigated. Furthermore, the preparing method of $terfenadine-{\beta}-\;cyclodextrin$ inclusion compound was setting up and its physico-chemical characteristics such as DSC curve, solubility, dissolution and anti-histaminic activity were investigated. In conclusion, the solubility of terfenadine was increasing ${\beta}-cyclodextrin$ and with the decreasing pH. $Terfenadine-{\beta}-cyclodextrin$ inclusion compound, whose yield is almost 100%, was prepared by neutralization method. This inclusion compound was 200-times as soluble as terfenadine in pH 1.2-6.8. In addition, it had the faster dissolution and anti-histaminic activity than terfenadine. Therefore, it is used to the active substance of solid dosage form such as tablet and capsule in stead of terfenadine.

• Journal of Ceramic Processing Research
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• 제19권5호
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• pp.413-418
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• 2018

All-solid-state lithium batteries (ASSBs) using inorganic sulfide-based solid electrolytes are considered prospective alternatives to existing liquid electrolyte-based batteries owing to benefits such as non-flammability. However, it is difficult to form a favorable solid-solid interface among electrode constituents because all the constituents are solid particles. It is important to form an effective electron conduction network in composite cathode while increasing utilization of active materials and not blocking the lithium ion path, resulting in excellent cell performance. In this study, a mixture of fibrous VGCF and spherical nano-sized Super P was used to improve rate performance by fabricating valid conduction paths in composite cathodes. Then, composite cathodes of ASSBs containing 70% and 80% active materials ($LiNi_{0.6}Co_{0.2}Mn_{0.2}O_2$) were prepared by a solution-based process to achieve uniform dispersion of the electrode components in the slurry. We investigated the influence of binary carbon additives in the cathode of all-solid-state batteries to improve rate performance by constructing an effective electron conduction network.

• 복식
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• 제56권8호
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• pp.15-34
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• 2006

Knit wear was industrialized, diversified, specialized and was made into high-class and it is including knitted cloth and small product for fashion as well as cardigan, sweater, and jacket etc. unlike past recognition only with underwear and inner wear. To consider elasticity to be the best strength of knit wear, middle-aged women that form change is serious are wearing knit wear as clothes for going out, and this may be regarded as clothes action to be conscious of form than vogue. Thus, in this study, this researcher tried to suggest solution scheme through the development of design of knit wear to supplement the form defect of middle-aged women. This researcher tried to develop the design of knit wear to supplement the form defect of middle-aged women, after examining the theoretical background of knit wear, the bodily and mental feature of middle-aged women. Internal and external knit wear brand that middle-aged women prefer as the scheme for solving the problematic form of middle-aged women with knit wear. As the result first, to see the present situation of inland production enterprise of knit wear, almost all the brand companies are concentrating energies on widening age class. Second, middle-aged women become far off from the form which is regarded ideal form in modern society. Third, result that this researcher examined the brand of knit wear of middle-aged women is as follows : MORADO, Cartknit, Escalier. And, in foreign knit wear brand, ST John, Missoni, ESCADA. Fourth, as the result that this researcher did work manufacture for the supplement of form defect of middle-aged women, it has practical and active side, and off-time has high-class and elegant refined beauty.

• 대한토목학회논문집
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• 제33권4호
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• pp.1315-1326
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• 2013

하중에 민감한 케이블을 사용하는 현수교의 기하학적 형상은 하중을 저항할뿐만 아니라 시지각적 실루엣이 된다. 이 연구에서는 정력학적 도해법이 지니는 두 가지 가능성을 이용해서 힘의 흐름을 따르는 현수교의 초기 구조형태계획법을 제시한다. 첫째, 힘다각형을 이용해서 동일한 힘에 대해서 다양한 하중경로를 모색할 수 있다. 둘째, 구조형태와 힘다각형 사이에 존재하는 상반원리를 이용해서 새로 형성된 하중경로에 대응하는 구조형태를 생성할 수 있다. 먼저 현수교의 구조적 및 미학적 측면에 영향을 미치는 주요 구조형태변수를 선정한다. 이러한 변수에 대해서 구조형태와 힘다각형의 상호관계를 분석한다. 이를 토대로 현수교의 초기 구조형태계획에 대한 일련의 설계과정절차들을 개발한다. 이 연구에서 제시하는 구조형태계획법은 유사 형태저항 구조시스템의 설계대안을 생성하는데 수월하게 확장해서 사용할 수 있다. 또한 구조형태와 힘의 흐름과의 상호관계를 설명하는 교육용 도구로도 사용될 수 있을 것으로 기대한다.

• 생명과학회지
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• 제12권4호
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• pp.477-482
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• 2002

효모 glucoamylase의 promoter와 분비신호서열 그리고 MF$\alpha$1의 prosequence를 이용하여 곤충 defensin을 S. cerevisiae 2805에서 항균활성을 보유한 형태로 발현 및 분비하는데 성공하였다. 발현된 defensin의 대부분이 세포 외로 분비되어 거의 모든 항균활성이 배양 상등액에 존재하였다. 이것은 S. cerevisiae에서 발현된 defensin이 glucoamylase의 분비신호서열과 MF$\alpha$1의 prosequenre에 의해 효율적으로 processing되어 분비됨을 시사한다. Defensin의 다른 미생물에 대한 항균활성을 조사한 결과 병원균인 St. aureus와 L. monocytogenes에 대해서도 항균활성이 존재하였다.

• 한국정밀공학회지
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• 제16권1호통권94호
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• pp.253-264
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• 1999

Most autonomous mobile robots view only things in front of them, and as a result, they may collide with objects moving from the side or behind. To overcome this problem. an Active Omni-directional Range Sensor System has been built that can obtain an omni-directional range data through the use of a laser conic plane and a conic mirror. Also, mobile robot has to know its current location and heading angle by itself as accurately as possible to successfully navigate in real environments. To achieve this capability, we propose a self-localization algorithm of a mobile robot using an active omni-directional range sensor in an unknown environment. The proposed algorithm estimates the current position and head angle of a mobile robot by a registration of the range data obtained at two positions, current and previous. To show the effectiveness of the proposed algorithm, a series of simulations was conducted and the results show that the proposed algorithm is very efficient, and can be utilized for self-localization of a mobile robot in an unknown environment.

• 영어어문교육
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• 제17권4호
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• pp.177-191
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• 2011

This paper examines nursing professionals' English abstract to learn their preferences towards tense and voice choices. A total of 24 abstracts, completed reviews to be published by the editorial board members of the Korea Industrial Nursing Association, were analyzed for the study. Each sentence in the four parts of the abstract (Purpose, Methods, Results, and Conclusion) was examined and classified into active/passive voices, and present/past/present perfect tenses. Verbs were then further identified to see which ones were commonly preferred to state the objectives of the study, methods, and to draw conclusions. Hedging expressions in Conclusion were also examined. The results of the present study revealed that Purpose was mostly (79%) stated in the past tense with slight use (17%) of the present tense in the form of 58% active and 42% passive voice whereas Methods were dominantly (96%) illustrated in the past tense with preference of mixed active and passive voice. The Results were also preferably (92%) stated in past tense and Conclusion in both present and past tense. Verbs used by these nursing professionals seemed diverse; however, hedging appeared to be narrowly limited to a few expressions including suggest and should. More diverse English hedging expressions need to be taught at least college level writing so that the EFL learners and writers can have a better understanding of presenting statements in an appropriate level of caution, confidence, or uncertainty.

• 소음진동
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• 제5권2호
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• pp.225-234
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• 1995

In this study, first, transformation process of damping ratios, whose are evaluated in active control analysis, into damping matrix resulting from installed viscous dampers is illustrated. Then, a method is followed to maximize the effect of response reduction, which leads to optimum locations and size of viscous dampers using sensitivity analysis. Highly coupled nonlinearity between damping ratios and dampers makes it hard to find the optimal size of dampers. Therefore, the nonlinearity is transformed to linear problem with small increments of damping ratios and the size of dampers can be found. However, there are many cases for the size of dampers satisfying the small increment of damping ratios, so it is necessary to select minimum size using optimization technique. To determine optimum locations of dampers, dampers are assumed to be installed between the different stories and their locations are selected corresponding corresponding to the degree of damping size. Numerical examples for the frame structure and the shear wall structure show that optimum locations and size of dampers are different form each other depending on the characteristics of modal responses of the structures. The proposed method in this study can be applied to get optimum locations of active controller in the active control.