• Smart Structures and Systems
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• 제20권6호
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• pp.657-668
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• 2017

In this research, an active vibration suppression of a smart beam having piezoelectric sensor and actuators is investigated by designing separate controllers comprising a linear quadratic regulator and a neural network. Firstly, design of a smart beam which consists of a cantilever aluminum beam with surface bonded piezoelectric patches and a designed mechanism having a micro servomotor with a mass attached arm for obtaining variations in the frequency response function are presented. Secondly, the frequency response functions of the smart beam are investigated experimentally by using different piezoelectric patch combinations and the analytical models of the smart beam around its first resonance frequency region for various servomotor arm angle configurations are obtained. Then, a linear quadratic regulator controller is designed and used to simulate the suppression of free and forced vibrations which are performed both in time and frequency domain. In parallel to simulations, experiments are conducted to observe the closed loop behavior of the smart beam and the results are compared as well. Finally, active vibration suppression of the smart beam is investigated by using a linear controller with a neural network based adaptive element which is designed for the purpose of overcoming the undesired consequences due to variations in the real system.

• Journal of Power Electronics
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• 제15권1호
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• pp.202-215
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• 2015

This paper investigates the stability and performance of model predictive controlled active-front-end (AFE) rectifiers for energy storage systems, which has been increasingly applied in power distribution sectors and in renewable energy sources to ensure an uninterruptable power supply. The model predictive control (MPC) algorithm utilizes the discrete behavior of power converters to determine appropriate switching states by defining a cost function. The stability of the MPC algorithm is analyzed with the discrete z-domain response and the nonlinear simulation model. The results confirms that the control method of the active-front-end (AFE) rectifier is stable, and that is operates with an infinite gain margin and a very fast dynamic response. Moreover, the performance of the MPC controlled AFE rectifier is verified with a 3.0 kW experimental system. This shows that the MPC controlled AFE rectifier operates with a unity power factor, an acceptable THD (4.0 %) level for the input current and a very low DC voltage ripple. Finally, an efficiency comparison is performed between the MPC and the VOC-based PWM controllers for AFE rectifiers. This comparison demonstrates the effectiveness of the MPC controller.

• Journal of Power Electronics
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• 제17권4호
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• pp.1058-1070
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• 2017

This paper proposes a novel active damping scheme to suppress LCL-filter resonance with only grid-current feedback control in grid-connected voltage-source converters. The idea comes from the concept of the model reference adaptive control (MRAC). A detailed theoretical derivation is given, and the effectiveness of this method is explained based on its physical nature. According to the control structure of this method, the active damping compensator, which is essentially a second order resonant integrator (SORI) filter, provides an effective solution to damp LCL resonance and to eliminate the need for additional sensors. Compared with extra feedback methods, the cost and complexity are reduced. A straightforward tuning procedure for the active damping method has been presented. A stability analysis is illustrated in the discrete domain while considering a one-step delay. Finally, experimental results are presented to validate the analysis and to demonstrate the good performance of the proposed method.

• 한국음향학회지
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• 제28권2호
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• pp.135-140
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• 2009

본 논문에서는 하이라이트 모델에 기반하여 능동소나의 표적신호를 합성하고, 합성된 신호를 이용하여 표적인식 실험을 수행하였다. 동일 표적이라도 표적의 자세각에 따라 다양한 형태의 파형을 갖는 신호가 합성되는데, 이에 대한 표적인식 결과를 알아보기 위해서 두 가지 방법으로 실험을 수행하였다. 하나는 고정된 여러 가지 자세각에 대한 표적신호에 대한 인식실험이고, 다른 하나는 임의의 자세각을 가지는 교신에 대만 인식 실험을 수행하였다. 인식실험을 위한 특징 인자로는 합성된 표적신호에 대해 시간영역에서 정합필터 및 포락선 검출을 통해 얻어지는 하이라이트 패턴을 사용하였으며, 패턴인식 기법으로는 다중클래스 SVM과 인공신경망을 사용하였다.

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.427-433
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• 2008

The cephabacins produced by Lysobacter lactamgenus are ${\beta}$-lactam antibiotics composed of a cephem nucleus, an acetate residue, and an oligopeptide side chain. In order to understand the precise implication of the polyketide synthase (PKS) module in the biosynthesis of cephabacin, the genes for its core domains, ${\beta}$-ketoacyl synthase (KS), acyltransferase (AT), and acyl carrier protein (ACP), were amplified and cloned into the pET-32b(+) expression vector. The sfp gene encoding a protein that can modify apo-ACP to its active holo-form was also amplified. The recombinant KS, AT, apo-ACP, and Sfp overproduced in the form of $His_6$-tagged fusion proteins in E. coli BL21(DE3) were purified by nickel-affinity chromatography. Formation of stable peptidyl-S-KS was observed by in vitro acylation of the KS domain with the substrate [L-Ala-L-Ala-L-Ala-L-$^3H$-Arg] tetrapeptide-S-N-acetylcysteamine, which is the evidence for the selective recognition of tetrapeptide produced by nonribosomal peptide synthetase (NRPS) in the NRPS/PKS hybrid. In order to confirm whether malonyl CoA is the extender unit for acetylation of the peptidyl moiety, the AT domain, ACP domain, and Sfp protein were treated with $^{14}C$-malonyl-CoA. The results clearly show that the AT domain is able to recognize the extender unit and decarboxylatively acetylated for the elongation of the tetrapeptide. However, the transfer of the activated acetyl group to the ACP domain was not observed, probably attributed to the improper capability of Sfp to activate apo-ACP to the holo-ACP form.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.64-64
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• 2003

The sex differentiation of fishes occurs under the control of genetic and various environmental factors. DM-domain containing genes are novel zinc finger transcription factors and play key roles in sex determination. In order to isolate the wrasse DMRT (wDMRT) cDNA from the protogynous wrasse (Halichoeres tenuispinnis), the wrasse testis cDNA library was screened using the $^{32}$ P-labeled PCR products, which were amplified with the degenerate primers from conserved DM-domain regions of several DMRT genes. Among a few positives obtained through screening, the full length wDMRT cDNA of 2.9kb size encoding a predicted 300 amino acid residues was isolated. The sequence analysis exhibited 60%, 43% sequence identity with rainbow trout and tilapia DMRT1, respectively. RT-PCR assay showed that wDMRT was expressed specifically in male testis. Also, wDMRT gene was strongly expressed in May during reproductive season, when the reproductivity of wrasse is most active. This results suggested that wDMRT gene function in testis differentiation The conserved DM-domain regions were amplified using PCR from DMRT genes of several species among Labridae, and their sequences were determined. The sequence of DM-domain region of Halichoeres. tenuispinis was identical to those of Pseudolabrus japonicus, Pteragogus flagellifera, and showed 94% identity with that of Halichoeres poecioptrerus.

• 디지털콘텐츠학회 논문지
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• 제10권4호
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• pp.551-560
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• 2009

최근의 글로벌 경영환경, 그리고 디지털 미디어를 통한 전자상거래가 활성화되고 있는 상황 하에서 수출기업들의 브랜드 및 웹 도메인 개발에 관한 의사결정은 매우 중요한 전략적 선택이다. 특히 웹 도메인 개발은 기업의 브랜드 전략과 밀접하게 관련되어 있기 때문에 브랜드 개발 시 동시에 개발되는 경우가 대부분이다. 따라서 본 연구에서는 한국수출기업의 해외 브랜드 및 웹 도메인 개발 전략에 관한 수요조사결과를 토대로 수출브랜드전략의 결정요인을 탐색적으로 고찰하기위하여 실행되었다. 이를 위하여 본 연구에서는 브랜드전략 결정요인으로 수출경쟁력, 시장의 유사성, 환경의 불확실성으로 구분하고 브랜드 전략과의 관계를 회귀분석 하였다. 연구결과 한국수출기업의 경우에 기대한 바와 같이 수출경쟁력이 높을수록 OEM브랜드 수출비중이 낮고, 환경의 불확실성이 클수록 OEM브랜드 수출비중은 높은 것으로 나타났다. 그러나 시장의 유사성은 통계적으로 유의한 영향을 미치지 못한 것으로 나타났다. 자사브랜드 전략을 사용하고 있는 기업의 경우에도 수출경쟁력이 높은 기업일수록 글로벌브랜드를 선택하는 경향이 높고 환경의 불확실성이 클 경우에는 로컬브랜드 전략을 선택할 가능성이 높은 것으로 나타나 가설을 지지하였다. 그러나 시장의 유사성은 글로벌브랜드 전략 또는 로컬브랜드 전략과의 유의미한 통계적 관계를 보여주지 못했다.

• 한국자기공명학회논문지
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• 제20권2호
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• pp.56-60
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• 2016

Adenylate kinase (AK) enzyme which acts as the catalyst of reversible high energy phosphorylation reaction between ATP and AMP which associate with energetic metabolism and nucleic acid synthesis and signal transmission. This enzyme has three distinct domains: Core, AMP binding domain (AMPbd) and Lid domain (LID). The primary role of AMPbd and LID is associated with conformational changes due to flexibility of two domains. Three dimensional structure of human AK1 has not been confirmed and various mutation experiments have been done to determine the active sites. In this study, AK1R138A which is changed arginine[138] of LID domain with alanine[138] was made and conducted with NMR experiments, backbone dynamics analysis and mo-lecular docking dynamic simulation to find the cause of structural change and substrate binding site. Synthetic human muscle type adenylate kinase 1 (hAK1) and its mutant (AK1R138A) were re-combinded with E. coli and expressed in M9 cell. Expressed proteins were purified and finally gained at 0.520 mM hAK1 and 0.252 mM AK1R138A. Multinuclear multidimensional NMR experiments including HNCA, HN(CO)CA, were conducted for amino acid sequence analysis and signal assignments of $^1H-^{15}N$ HSQC spectrum. Our chemical shift perturbation data is shown LID domain residues and around alanine[138] and per-turbation value(0.22ppm) of valine[179] is consid-ered as inter-communication effect with LID domain and the structural change between hAK1 and AK1R138A.

• 재활간호학회지
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• 제4권1호
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• pp.94-104
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• 2001

The purpose of this study was to analysis of nursing interventions performed by the ICU nurses to the patients with cerebrovascular disorder practically from one university hospital in Seoul. The data were collected from 15 nurses with 86 cerebrovascular disorder cases from one ICU with the questionaire to write frequency of nursing care done by the surveyee from May, 2, 2000 to July, 3, 2000 and the list of 66 nursing interventions selected out of 433 NIC(Nursing Interventions Classification) of Iowa University which were translated into Korean (44 items) and core nursing interventions by ICU nurses (22 items; KIm, Su-Jin, 1997). The data were analysed with SPSS program. The results are as follow : 1. The most frequently used nursing interventions were vital sign monitoring, fall prevention, cerebral edema management, dysreflexia management, neurologic monitoring, cardiac care, communication enhancement, technology management, bed rest care, respiratory monitoring in rank. 2. The most frequently used nursing intervention domains were 'Physiological : Complex', 'Physiological : basic', 'Behavior', 'Safty', 'Health system' in rank. In the domain of physiological : basic, the most frequently used nursing interventions were bed rest care, urinary elimination management, tube care : urinary, physical restraints in rank. In the domain of physiological : complex, the most frequently used nursing interventions were cerebral edema management, dysreflexia management, neurologic monitoring, cardiac care in rank. In the domain of behavior, the most frequently used nursing interventions were communication enhancement, touch, active listening in rank. In the domain of safty, the most frequently used nursing interventions were vital sign monitoring, fall prevention in rank. In the domain of health system, the most frequently used nursing interventions were technology management, specimen management in rank. 3. some difference of the frequency practicing the nursing interventions according to the shift of duties was found. For example, medication administration was common at day duty, touch was practiced at evening duty, temperature regulation was performed.

• Molecules and Cells
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• 제44권1호
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• pp.1-12
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• 2021

The nuclear receptor peroxisome proliferator-activated receptor γ (PPARγ) is the master transcriptional regulator in adipogenesis. PPARγ forms a heterodimer with another nuclear receptor, retinoid X receptor (RXR), to form an active transcriptional complex, and their transcriptional activity is tightly regulated by the association with either coactivators or corepressors. In this study, we identified T-cell death-associated gene 51 (TDAG51) as a novel corepressor of PPARγ-mediated transcriptional regulation. We showed that TDAG51 expression is abundantly maintained in the early stage of adipogenic differentiation. Forced expression of TDAG51 inhibited adipocyte differentiation in 3T3-L1 cells. We found that TDAG51 physically interacts with PPARγ in a ligand-independent manner. In deletion mutant analyses, large portions of the TDAG51 domains, including the pleckstrin homology-like, glutamine repeat and proline-glutamine repeat domains but not the proline-histidine repeat domain, are involved in the interaction with the region between residues 140 and 506, including the DNA binding domain, hinge, ligand binding domain and activation function-2 domain, in PPARγ. The heterodimer formation of PPARγ-RXRα was competitively inhibited in a ligand-independent manner by TDAG51 binding to PPARγ. Thus, our data suggest that TDAG51, which could determine adipogenic cell fate, acts as a novel negative regulator of PPARγ by blocking RXRα recruitment to the PPARγ-RXRα heterodimer complex in adipogenesis.