• 제목/요약/키워드: active bleeding

검색결과 112건 처리시간 0.023초

비가역성 실혈성 쇽에서 본 가토심근, 혈장의 전해질 및 혈당량 변화 (Changes in Water and Electrolyte Distribution and Blood Glucose Concentration following Irreversible Hemorrhagic Shock)

  • 김기환;남기용
    • The Korean Journal of Physiology
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    • 제2권1호
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    • pp.47-52
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    • 1968
  • Twenty white rabbits anesthetized with nembutal (30 mg/kg) were employed in this experiment. Five of them served as controls; the remaining rabbits as experimental group were subjected to irreversible hemorrhagic shock. Shock was induced by bleeding the animals until mean blood pressure decreased to a level of 50-40 mmHg. This level of pressure was maintained for 3-4 hours, after which the drawn blood was reinfused. The reinfusion of blood caused the elevation of arterial pressure almost the control level for some minutes, after which a gradual and progressive decline of blood pressure became evident. This decline was thought to be the result from irreversible hemorrhagic shock. When mean blood pressure declined to less than 50 mmHg, chest was opened and samples of arterial blood and left ventricular muscle were taken. Left ventricular muscle and blood plasma were analyzed for potassium, sodium, chloride and water content. Blood glucose concentration was determined by Somogyi-Nelson's method. Extracellular and intracellular myocardial water and electrolyte content were calculated on the basis that electrolytes are distributed between plasma water and interstitial water according to Gibbs-Donnan equilibrium. In this calculation extracellular water was substituted for Na space. The findings obtained were as follows: 1. The concentration of blood glucose was 87mg% in the controls and it rose to 222 mg% in shock (P<0.01). 2. Plasma potassium elevated significantly from 3.3 mEq/l in controls to 8.0 mEq/l in shock (P<0.01), while small decreases in sodium (151-146 mEq/l) and chloride (102-96 mEq/l) were observed (P<0.3, P<0.1), 3. The changes of blood water content (83.1-84.3%) and cardiac water content (77.5-78.3 gm/100gm WT) were observed. 4. In control animals myocardial potassium levels which averaged 30.2 mEq/100 gmDT rose significantly to 40.3 mEq/100 gmDT in shock (P<0.01), while moderate decreases in sodium(16.3-14.3 mEq/100 gmDT) were observed in shock. 5. The calculated transmembrane resting potential of left ventricular muscle of control animals averaged 95 mV, while rabbits in shock averaged 77 mV. (P <0.01). The findings of this experiment do not correspond with the conclusions that myocardial depression seems to be the cause of irreversible hemorrhagic shock, because the excitability of heart muscle is elevated. From the point of view that the lowered transmembrane resting potential, the cause of death in terminal stage of irreversible hemorrhagic shock may be ventricular fibrillation. It can't be said, however, that the lowered transmembrane resting potential is responsible for the transition from reversible to irreversible hemorrhagic shock. The marked increase in blood glucose suggested that glycogenolysis in the liver is favorably active in shock.

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치주 유지관리기 환자에게 PRF-K2를 함유한 nutraceutical의 효과 (The effect of nutraceutical containing PRF-K2 on periodontal condition during maintenance phase)

  • 김유강;정현주;김세원;백동현
    • Journal of Periodontal and Implant Science
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    • 제37권1호
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    • pp.91-102
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    • 2007
  • The long term success of periodontal treatment is dependent upon the effectiveness of the main-tenance care program after active treatment. The purpose of this study was to evaluate whether nutraceutical containing PRF-K2 as natural product from plant and seaweed has beneficial effects on clinical parameters, gingival crevicular fluid (GCF) volume and GCF cytokine levels during main- tenance phase after periodontal treatment. Among the generally healthy and non-smoking. moderate to severe chronic periodontitis patients during maintenance phase in Department of Periodontics, Chonnam National University Hospital, twenty eight patients took nutraceutical containing PRF-K2 (Oscotec Inc. Cheonan, Korea) for 3 months as experimental group and sixteen patients received only maintenance care as control group. Clinical examination and GCF collection were performed at baseline, 1, 2 and 3 months of experiment. Total amounts and concentrations of GCF IL-1{\beta}, IL-1ra and $PGE_2$ were evaluated using ELISA kit. In probing pocket depth, experimental group showed the tendency of more reduction than control group after 3 months of experiment. Sulcus bleeding index (SBI) and GCF volume were significantly decreased in experimental group(p<0.05), whereas they were increased in control group. GCF IL-1{\beta} level tended to decrease in both experimental and control group and IL-1ra concentration tended to increase in experimental group and to decrease in control group. IL-1ra/IL-1{\beta} ratio tended to increase in experimental group and to decrease in control group during experimental period. GCF $PGE_2$ amount did not show any change in experimental group and tended to increase in control group. These results suggest that nutraceutical supplement which contain PRF-K2 could improve perio-dontal condition during maintenance phase after periodontal therapy.

하악 후방 견인력이 성장기 유견의 하악과두에 미치는 영향에 관한 실험적 연구 (AN EXPERIMENTAL STUDY ON THE EFFECT OF THE MANDIBULAR RETRACTIVE FORCE ON THE MANDIBULAR CONDYLE OF GROWING YOUNG DOG)

  • 임용규;양원식
    • 대한치과교정학회지
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    • 제20권2호
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    • pp.363-379
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    • 1990
  • The purpose of this study was to evaluate the effects of the mandibular retractive force on the mandibular condyle of growing dog. The experimental animals were six mongrel dogs of two-month old. Their deciduous dentition were completed. Two of them was used as control group, and experimental group was composed of remaining four. Head band and chin cup were made of cotton tape, and hooks are fabricated on the chin cup and had band for closed coil. Mandibular retractive force was 100g/side and chin cap appliance was used for 14 hours/day during night. Experimental group were sacrificed at 2, 4, 6, 8 weeks from beginning of the experiment. Right TMJ was prepared for histologic study and left TMJ was examined grossly for disc, fossa, and condyle. The conclusions are: 1. Two-month old control animal showed active cartilaginous growth on the mandibular condyle, therefore showed thick proliferative and hypertrophic zones. Remodeling process in the condyle head was observed in which there were bone resorption on the anterior surface and bone apposition on the posterior surface. 2. Four-month old control animal showed marked reduction of hypertrophic zone but the condylar bone remodeling was more pronounced. 3. In experimental group, there are marked reduction of hypertrophic zone at 4 weeks from beginning of experiment, and hypertrophic zone disappeared at posterior-superior portion of condyle in 6-week experimental animal. 8 week experimental animal showed slight recovery of hypertrophic zone. 4. In experimental group, bone deposition was increased at anterior surface of condyle, and bone resorption was increased at posterior surface of condyle. 5. In control group, the glenoid fossa and surrounding bone showed mainly bone apposition. But experimental group showed bone resorption at anterior surface of articular eminence and increased bone apposition at posterior surface of postglenoid spine. 6. No marked traumatic change was seen but 4 weeks and 8 weeks experimental animal showed flattening of posterior surperior condylar surface. Bone marrow of condyle showed minute focal bleeding in 2 weeks and 4 weeks experimental animal, and congestion and depression of hematopoietic bone marrow during all experimental period.

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구불창자간막 혈종으로 발현한 회충증: 증례 보고 (Ascariasis Presenting as Hematoma in the Sigmoid Mesocolon: A Case Report)

  • 김지은;배경은;김현정;배병노;이지혜;강미진;김지영;김재형
    • 대한영상의학회지
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    • 제81권4호
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    • pp.1013-1018
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    • 2020
  • 회충증은 Ascaris lumbricoides에 의하여 유발되는 장 질환이다. 대부분의 회충증 환자는 무증상이지만 장내 유충이 많은 경우에는 장 폐색, 폐쇄성 황달, 담관염, 담낭염, 췌장염과 같은 합병증이 생길 수 있다. 저자들은 컴퓨터단층촬영(이하 CT)에서 구불창자간막에 혈종과 활동성 출혈이 발생하여 회충증으로 진단된 74세 남자의 사례를 경험하였다. 이후에 환자가 시행한 CT에서는 이와 더불어 구불창자의 천공 발생이 확인되었다. 복강경을 통한 저위전방절제술이 시행되었고 구불창자간막에 큰 혈종이 있었으며 장간막 지방 조직에서 현미경적으로 회충이 확인되었다. 이러한 회충증의 드문 임상적, 영상의학적 소견은 기생충이 장관의 내강 안에서 복강 내로 이동하는 과정에서 발생한 순차적인 합병증들을 보여준다.

후천성 면역 결핍 바이러스와 연관된 위장관 카포시 육종: 증례 보고 (Human Immunodeficiency Virus-Associated Gastrointestinal Kaposi's Sarcoma: A Case Report )

  • 임희중;박소현;최승준;박수영;이희영;정준원;정동해
    • 대한영상의학회지
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    • 제81권5호
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    • pp.1260-1265
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    • 2020
  • 카포시 육종은 후천성 면역 결핍 증후군(acquired immunodeficiency syndrome; 이하 AIDS)와 연관되어, 피부, 점막과 여러 장기를 침범하는 다발성의 혈관성 결절로 나타나는 신생물을 일컫는다. AIDS와 연관되어 생기는 위장관 카포시 육종은 내장에 파종성 질환으로 가장 흔하게 발생한다. 우리는 다양한 장기를 침범하여 복통과 함께 장관 내 출혈을 유발한 드문 카포시 육종의 영상 소견에 대해 보고한다. 회장 대장내시경을 통해 말단 회장, S자 결장, 직장 내 다양한 병변이 발견되었으며 소장 투시검사로 공장과 회장의 병변을 확인할 수 있었다. 복부 골반 전산화단층촬영에서 회장 내 조영증강된 다양한 납작한 병변과 복강 내 크기가 커진 림프절을 발견하였다. 조직병리학 검사에서 카포시 육종으로 최종 진단이 된 환자는 항레트로바이러스제로 치료를 시행 받았다. 최근 AIDS 환자의 수가 늘고 있는 상황에서, AIDS와 연관된 Kaposi's sarcoma의 영상학적 소견을 숙지하는 것은 영상의학과 의사를 비롯한 임상의사들에게 빠른 진단과 치료뿐만이 아니라 예상치 못한 AIDS의 감염 위험에 노출될 수 있는 의료진의 안전을 위해서도 필수적이다.

MDBK 세포 배양에서 Eimeria tenella 발육 상황 및 닭 비장세포에 의한 발육 항진 효과 (Development of Eimeriu tenezla in MDEK cell culture with a note on enhancing effeet of preincubation with chicken spleen cells)

  • 채종일;이순형
    • Parasites, Hosts and Diseases
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    • 제27권2호
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    • pp.87-100
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    • 1989
  • 닭의 맹장 상피세포에 기생하여 출혈, 설사 등을 일으키는 구충류(구충류. Coccidia인 Eimerin tenella는 감염 초기에 상피 내 림프구(intraepithelial Lymphocyte; IEL)와 같은 면역계 세포로 먼저 침입하는 것이 잘 알려져 있다. 이 연구는 E. tenella가 면역계 세포 내로 들어가는 것이 추후 충체 생존 및 발육에 도움이 되는 과정인지를 알아보고자 한 것으로, 분리하기 쉬운 닭의 비장 세포를 면역계 세포로 이용하고 MDBK(Madin-Darby bovine kidney) 세포를 시험관 내(in nitre) 숙주세포로 하여 E. tenella의 생존 및 발육 능력을 정량적으로 측정한 것이다. 실험 과정은 3단계로 나누어 첫째 MDBK 세포가 숙주세포로 적합한지 우선 형태학적으로 관찰한 후, 둘째 E. tvnella sporozoites의 발육, 중식 상황을 3H-uracil 동위원내 흡수시험으로 정량화할 수 있는지 알아보고, 셋째 이 방법을 이용하여 sporozoites를 비장세포와 혼합배양한 후에는그 발육, 중식 능력이 어떻게 변화하는지 관찰하였다. MDBK 세포에 탈낭 직후의 정상 sporozoites를 직접 감염시켜 발육상황을 형태학적으로 관찰한 바 감염 3∼4일에 영양형 및 분열체(schizonts)가 완성되고 merosoites가 터져 나와 다른 세포로 침입하는 분열증식(schizogony)이 활발히 영위되고 있는 것이 확인되었다. 또 3H-uracil 흡수는 감염 12시간부터 뚜렷이 나타났고 48∼60시간에 peak에 달한 다음 서서히 감소하였다. 3H-uracil 흡수량은 sporozoites 감염 농도에 따라서도 변화하였으나 탈낭 후 MDBK 세포 감염까지의 시간 경과 에 따라 현저히 감소하였다. 그러나 sporozoites를 비장세포와 함께 4∼12시간 시험관 내에서 먼저 혼합배양한 다음 MDB노 세포에 감염시켰을 때 3H-uracil 흡수량은 sporozoites만을 태양한 대조군 에 비해 항상 높아 비장세포와의 혼합배양 후 발육증식이 더욱 활발해지는 것으로 나타났다. 이상의 결과로 보아 E. tenella의 sporosoites가 생체 내에서 IEL과 같은 면역계 세포에 침입하는 것이 충체 생존이나 발육에 있어서 비록 필수적인 것은 아니지만 자신의 생존이나 발육능력을 보존 또는 항진시키는데에 도움을 주는 과정의 하나로 판단되었다.

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흰쥐에서 혈액지질 대사 및 항혈전작용에 관한 베타사이클로덱스트린의 생물학적 효과 (The Biological Effects of β-Cyclodextrin on Antithrombotic Activity and Plasma Lipid Metabolism in Rats)

  • 박병성
    • Journal of Animal Science and Technology
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    • 제45권2호
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    • pp.199-210
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    • 2003
  • 일반사료, 고콜레스테롤사료 또는 포화지방함량이 높은 사료를 섭취한 흰쥐에서 항혈전작용과 혈액중성지방 및 콜레스테롤 수준에 관한 $\beta$-cyclodextrin($\beta$CD)의 급여효과를 조사하였다. 출혈시간은 일반사료, 고콜레스테롤 및 고지방사료 섭취군 모두에서 $\beta$CD 첨가구가 대조구에 비해서 각각 293%, 157% 및 218% 까지 유의적으로 연장되었다(p<0.05). 전혈응고시간은 일반사료, 고콜레스테롤 및 고지방사료섭취군에서 $\beta$CD 첨가구가 대조구와 비교할때 각각 202%, 168% 및 211%까지 유의적으로 높았다(p<0.05). $\beta$CD 첨가사료는 흰쥐 혈액내 총지질, 중성지방, 총콜레스테롤 및 저밀도지질단백질 콜레스테롤 함량을 현저하게 낮추는 원인이 되었다. 혈액내 총지질 함량은 일반사료, 고콜레스테롤 및 고지방사료 섭취군에서 $\beta$CD첨가구가 대조구에 비해서 각각 70%, 82% 및 87%까지 유의적으로 낮아졌다(p<0.05). 혈액내 중성지방함량은 일반사료, 고콜레스테롤 및 고지방사료 섭취군에서 $\beta$CD 첨가구가 대조구와 비교할 때 각각 89%, 43% 및 59%까지 유의적인 감소를 나타냈다(p<0.05). 혈액내 총콜레스테롤 함량은 일반사료, 고콜레스테롤 및 고지방사료 섭취군에서 $\beta$CD 첨가구가 대조구에 비해서 각각 28%, 62% 및 36%까지 유의적으로 낮아졌다(p<0.05). 혈액내 저밀도 지질단백질 콜레스테롤 함량은 일반사료, 고콜레스테롤 및 고지방사료섭취군에서 $\beta$CD 첨가구가 대조구에 비해서 각각 39%, 54% 및 25%까지 유의적인 감소를 나타냈다(p<0.05). 혈액내 총담즙산 함량은 일반사료, 고콜레스테롤 및 고지방사료섭취군에서 $\beta$CD 첨가구가 대조구에 비해서 각각 66%, 95% 및 97% 까지 유의적인 증가를 보였다(p<0.05). 일반사료 섭취군에서 $\beta$CD 첨가구의 HMG-CoA reductase 활성도는 대조구와 비교할 때 41%까지 유의적으로 낮아졌으며 분을 통한 스테로이드의 배설량은 167%까지 유의적인 증가를 나타냈다(p< 0.05). 이 결과는 $\beta$CD가 사람의 심장혈관계질환과 비만을 예방하는데 도움이 될 수 있는 혈액내 지질, 중성지방 및 콜레스테롤의 감소효과 그리고 항혈전작용에 관한 생리활성 기능을 갖는다는 것을 시사해 주고 있다.

장티브스에 관한 임상적 관찰 (Clinical review of Typhoid Fever Patients)

  • 최정신
    • 대한간호학회지
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    • 제6권1호
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    • pp.60-71
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    • 1976
  • The author reviewed the medical records of 96 typhoid fever patients who were diagnosed, admitted, and treated at Sea grave Memorial Hospital from January 1 , 1973 through August 31, 1975. Diagnosis was determined by clinical observation, aerology and bacteriology, eighty patients were treated medically, the remaining 16 patients required surgical intervention. The following results were obtained: 1) The age distribution of the patients revealed that 33.3% wert between 10 and 19 years old 21.9% were between 20 and 29, and 19.8% were between 30 and 39. The majority of patients were from these more active age groups. Male to female sex ratio was 1.3 : 1 2) Seasonal distribution was observed. Most illness occurred in the summer and autumn month 5. 3) 84. 3%of the patients came from farm families. 4) Duration between onset and admission averaged 16.0 days. The group without compilations was admitted after an average of 15. 1 days; The group with complications was ad-matted after an average of 19.4 days. 5) Methods of treatment before admission were as follows: 10.4% at medical clinics, 61, 5% at pharmacies (antibiotics 47.9%, other. drugs 13.5%), 7.3% by herb medications, 20.8% had no treatment. 6) Main clinical symptoms were as follows: fever 93.8%, headache 47.9%, abdominal pain 47.9%, chills 38.5%, cough 36.5%, general weakness 26.0%, nausea e vomiting 24.0% and generalized pain 21.9%. 7) Temperature of patients on admission: 22.9% were 39f or more, 67.6% were between 37℃ and 38℃, and 9.4% were 37℃ or less. 8) Occurrence of intensional bleeding after onset of disease averaged 9.3 days; perforation occurred at an average of 19. 1 days. 9) Interval between onset of major complication and surgical intervention averaged 2.8 days. 10) Among the 68 patients who underwent the bacteriological test the positive rate was 44.1% (30). The positive ,ales to, each separate culture method were as follows: 20.4% in the blood culture, 40.4% in the stool culture and 6.7% in the urine culture. Among these bacteriological positive patients 15 patients had a negative results or less than 160 titer of vidal reaction. 11) The initial vidal test of the total group showed a counts of 160 titer or more in 60.4% and less than 160 titer in 39.6%, 12) W. B. C. Counts in the uncomplicated group indicated that 32.5% were 6,000/㎣ or less, 47.5% were between 6,000 and 10,000, arid 20.0% were 10,000/㎣ or more. In the complicated group, 37.6% were 6,000/㎣ or less, 25,0% were 6,000-10,000/㎣ and 37.6% were 10,000/㎣ or more. 13) Duration of hospital stay of the patients averaged 6.4 days in the uncomplicated group and 12.7 days in the complicated group. 14) Subdiaphragmatic free air simple X-ray was found in 91.7% of the perforated cases. 15) Duration of antibiotic therapy until an febrile state was attained averaged 4.8 days in the uncomplicated group and 6.5 days in the complicated group. 16) Operative procedures were as follows: one layer simple closure of their perforation with or without debasement in 56.3%, drainage only in 6.3%, small bowel resection with primary anastomosis in 18.8% , externalization in 6.3%, cholecystectomy in 6.3%, The clinical findings of this study suggest the following recommendations. According to Top's report; 1% of typhoid fever patients treated with chlorarnphenicol and 2% of patients treated with other drugs become chronic carriers. Therefore, importance should be given to the strict control of these carriers. Immunization, improvement of sanitation and living standards are all needed for the prevention and treatment of disease, but a more serious problem is a lack of knowledge on the part of patients and their families. Thus it is most urgent to enlighten the citizens about the transmission and hygiene related to contagious disease. Legal restriction of sale of antibiotics at drug stores without a physician's prescription is an urgent matter for public health administrators. An even more important nursing responsibility is the reemphasis on health education both in the clinical setting and in the home.

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대량 객혈 환자에서 동맥 색전술의 치료 효과 (Transcatheter Arterial Embolization in the Treatment of Massive Hemoptysis)

  • 최완영;최진원;임병성;신동호;박성수;이정희;서홍석
    • Tuberculosis and Respiratory Diseases
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    • 제39권1호
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    • pp.35-41
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    • 1992
  • 연구배경 : 대량 객혈은 내과적 혹은 외과적 치료로도 사망율이 매우 높은 임상증상이다. 대량 객혈은 높은 기관지 동맥 혹은 비기관지성 전신동맥에서 출혈된다고 알려져 있으므로, 최근 상기 혈관들을 색전시키는 것이 치료방법으로 되고 있으나 초기 색전후 일부에서 객혈이 재발되는 것이 문제로 되어 있다. 이에 저자들은 대량출혈환자에서 동맥색전을 하여 즉각적인 지혈정도를 알아보고, 즉시 지혈된 후 객혈이 재발되는 환자들의 임상소견 및 초기 동맥촬영소견을 조사하여 보았다. 방법 : 1988년 1월부터 1991년 7월까지 전부 21명의 환자에서 대량 객혈로 동맥색전술을 시행하였다. 환자 모두에서 대퇴통맥으로 경피하 삽관술을 하여 기관지동맥외에도 비기관지 전신동맥에 혈관촬영을 시행한 후, 병변부위의 혈관에 gelfoam pieces로 색전을 실시하였다. 이후 환자들을 추적관찰하면서 동맥색전술의 효과를 평가하였다. 결과 : 처음 동맥색전후 21명의 전환자에서 즉시 지혈은 성공하였다. 9명의 환자에서 출혈이 재발되었으며, 12예의 환자들에서는 객혈의 재발은 없었다. 합병증으로는 발열, 흉통, 기침, 배뇨 장애, 장마비, 하지마비 및 비장경색증등이 있었으며, 9명의 환자에서는 별 증상을 호소하지 않았다. 출혈이 재발된 환자중, 2명은 대량객혈로 사망하였고, 2명은 폐절제술을 시행받았으며, 4명은 매번 객혈이 재발시마다 반복적인 동맥색전술 혹은 내과적 보존치료를 받고 있다. 결론 : 재출혈의 가능성이 있기는 하지만, 동맥색전술은 대량 객혈환자에서 출혈을 비교적 빠르고 안전하게 멈출 수 있는 치료법이다. 또 수술적 치료가 불가능한 폐질환 환자에서 객혈이 재발시마다, 반복 시행하는 동맥색전술은 일단 급한 출혈을 막고 생명을 연장시키는 데에 도움을 주리라 사료된다.

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배양골세포 이식이 치조골재생에 미치는 영향 (Effects Of Cultured Bone Cell On The Regeneration Of Alveolar Bone)

  • 정순준;허익;박준봉;이만섭;권영혁
    • Journal of Periodontal and Implant Science
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    • 제26권1호
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    • pp.1-26
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    • 1996
  • This study was performed to estimate the effects of cultured bone cell inoculated on porous type hydroxyaptite for the regeneration of the artificial alveolar bone defect. In this experiment 3 beagle dogs were used, and each of them were divided into right and left mandible. Every surgical intervention were performed under the general anesthesia by using with intravenous injection of Pentobarbital sodium(30mg/Kg). To reduce the gingival bleeding during surgery, operative site was injected with Lidocaine hydrochloride(l:80,000 Epinephrine) as local anesthesia. After surgery experimental animal were feeded with soft dietl Mighty dog, Frisies Co., U.S.A.) for 1 weeks to avoid irritaion to soft tissue by food. 2 months before surgery both side of mandibular 1st premolar were extracted and bone chips from mandibular body were obtained from all animals. Bone cells were cultured from bone chips obtained from mandible with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. Porous type hydroxyapatite were immerse into the high concentrated cell suspension solution, and put 4 hours for attachin the cells on the surface of hydroxyapatite. Graft material were inserted on the artificial bone defect after 3 days of culture. Before insertion of cellinoculated graft material, scanning electronic microscopic observation were performed to confirm the attachment and spreading of cell on the hydroxyapatite surface. 3 artificial bone defects were made with bone trephine drill on the both side of mandible of the experimental animal. First defect was designed without insertion of graft material as negative control, second was filled with porous replamineform hydroxyapatite inoculated with cultured bone marrow cells as expermiental site, and third was filled with graft materials only as positive control. The size of every artificial bone defect was 3mm in diameter and 3mm in depth. After the every surgical intervention of animals, oral hygiene program were performed with 1.0% chlorhexidine digluconate. All of the animals were sacrificed at 2, 4, 6 weeks after surgery. For obtaining histological section, tissus were fixed in 10% Buffered formalin and decalcified with Planko - Rycho Solution for 72hr. Tissue embeding was performed in paraffin and cut parallel to the surface of mandibular body. Section in 8um thickness of tissue was done and stained with Hematoxylin - Eosin. All the specimens were observed under the light microscopy. The following results were obtained : 1. In the case of control site which has no graft material, less inflammatory cell infiltration and rapid new bone forming tendency were revealed compared with experimental groups. But bone surface were observed depression pattern on defect area because of soft tissue invasion into the artificial bone defect during the experimental period. 2. In the porous hydroxyapatite only group, inflammatory cell infiltration was prominet and dense connective tissue were encapsulated around grafted materials. osteoblastic activity in the early stage after surgery was low to compared with grafted with bone cells. 3. In the case of porous hydroxyapatite inoculated with bone cell, less inflammatory cell infiltration and rapid new bone formation activity was revealed than hydroxyapatite only group. Active new bone formation were observed in the early stage of control group. 4. The origin of new bone forming was revealed not from the center of defected area but from the surface of preexisting bony wall on every specimen. 5. In this experiment, osteoclastic cell was not found around grafted materials, and fibrovascular invasion into regions with no noticeable foreign body reaction. Conclusively, the cultured bone cell inoculated onto the porous hydroxyapatite may have an important role of regeneration of artificial bone defects of alveolar bone.

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