• 한국화재소방학회:학술대회논문집
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• 한국화재소방학회 1997년도 International Symposium on Fire Science and Technology
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• pp.104-111
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• 1997

Cluster systems are microcrystals of vanadiumoxided compounds such as Barium, Calcium or Sodium Metavanadates or Sodium Vanadium Bronze which are distributed into dimethyl- or diethylphosphites or microcrystals of vanadium oxides, for instance, vanadium oxide (+3), distributed into the methylphosphonic acid melted. During the interaction of vanadium compounds with the correspondent phosphororganic substances biue viscous liquids are formed. These liquids have paramagnetic properties. According to the UV and IR spectroscopic investigations as well as the results of EPR spectra the substances obtained consist of the nucleus containing 6 to 12 of vanadium atoms and the shell including ligands which are molecules of phosphites or methylphosphonic acid. Here every atom of vanadium interacts with four of phosphorus containing molecules. Sizes of the particles in these systems donot exceed 200 nm. Introduction of cluster systems (0,1 -0,3 % vanadium) into epoxy compositions before the introduction of curing agent - polyethylenepolyamine 6 -8 % leads to the acceleration of composition crosslinking and to the combustion decreasing: 1) Oxygen Index grows to 35: 2)mass losses during combustion decrease to 1-2%, 3) combustion time does not exceed 1 s; 4) the intumescence of material sample is being observed during the burner action as well as the foam coke formation.

• Biomolecules & Therapeutics
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• 제27권6호
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• pp.540-552
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• 2019

To determine the chemopreventive potential of alyssin and iberin, the in vitro anticancer activities and molecular targets of isothiocyanates (ITCs) were measured and compared to sulforaphane in hepatocellular carcinoma cell HepG2. The SR-FTIR spectra observed a similar pattern vis-a-vis the biomolecular alteration amongst the ITCs-treated cells suggesting a similar mode of action. All of the ITCs in this study cause cancer cell death through both apoptosis and necrosis in concentration dependent manner ($20-80{\mu}M$). We found no interactions of any of the ITCs studied with DNA. Notwithstanding, all of the ITCs studied increased intracellular reactive oxygen species (ROS) and suppressed tubulin polymerization, which led to cell-cycle arrest in the S and $G_2/M$ phase. Alyssin possessed the most potent anticancer ability; possibly due to its ability to increase intracellular ROS rather than tubulin depolymerization. Nevertheless, the structural influence of alkyl chain length on anticancer capabilities of ITCs remains inconclusive. The results of this study indicate an optional, potent ITC (viz., alyssin) because of its underlying mechanisms against hepatic cancer. As a consequence, further selection and development of effective chemotherapeutic ITCs is recommended.

• 한국어병학회지
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• 제9권1호
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• pp.65-77
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• 1996

Tachyplesin I은 투구게로부터 단리된 항균성 펩티드이다. 인지질막에 대한 tachyplesin I의 작용 메카니즘을 조사하기 위해서 tachyplesin I 및 5개의 유도체를 액상법으로 합성하였다. 합성한 5개의 유도체는 [$Phe^2$]-tachyplesin I, [$Phe^{8,13}$]-techyplesin I, S-S결합을 가지지 않는 [$Cys(Acm)^{3,7,12,16}$]-tachyplesin I 및 [$Cys(Acm)^{3,7,12,16}$]-tachyplesin I 의 단편인 7(Acm)과 10(Acm)이다. 원편광이색성 (CD) 스펙트럼에서 tachyplesin I은 완충액에서 역평행 $\beta$-구조를 취하며 산성 지질막하에서는 완충액보다 약간 불규칙적인 구조를 가진다. Carboxyfluorescein 누출 실험결과 tachyplesin은 중성 및 산성 지질막과 강하게 상호 작용 하였다. 또한 형광 실험하에서는 펩티드의 소수성 부분이 인지질막의 내부에 존재하였다. 7(Acm) 및 10(Acm) 유도체를 제외한 모든 펩티드들은 lipopolysaccharide결합에 있어 거의 유사한 활성을 나타냈었다. 따라서 지질이중막은 tachyplesin I을 안정한 $\beta$-구조로부터 덜 규칙적인 구조로 구조적 변화를 유도한다고 여겨진다.

• 생명과학회지
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• 제24권8호
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• pp.873-879
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• 2014

소나무(Pinus densiflora)의 잎의 성분을 분리하여 항트롬빈 활성을 조사하였다. 잎은 70% ethanol로 3회 추출하였으며, 그 추출물은 순차적으로 chloroform과 n-buthanol로 분획하였다. n-buthanol 분획으로부터 얻은 수용성 분획을 MPLC와 HPLC에 의해 분리하였다. 분리한 compound를 $^1H-$과 $^{13}C-NMR$로 동정한 결과 2, 3-dihydro-7-hydroxyl-3-hydroxymethyl-2-(4'-hydroxyl-3-methoxyphenyl)-5-benzofuranpropanol-3-O-${\alpha}$-rhamnopyranoside(a neolignan glycoside)와 2, 3-dihyro-3-hydroxymethyl-7-methoxy-2-(4'-hydroxyphenyl-3'-methoxy)-5-benxofuran propanol 4'-O-${\alpha}$-rhamnopyranoside (icariside $E_4$)의 neolignan 구조임을 확인하였다. 트롬빈 저해활성은 분리된 neolignan glycoside와 icariside $E_4$을 작용하여 혈장안에서 응고시간으로 측정하였다. 그 결과, neolignan glycoside의 응고시간이 icariside $E_4$보다 4배 이상 지연하였다. 저해활성은 neolignan glycoside의 농도가 증가함에 따라 그 시간이 지연되는 것을 확인하였다. 더 나아가 지연기전을 확인하기 위해 thrombin과 순수한 fibrinogen를 반응하였다. 그 결과, 트롬빈과 순수한 피브리노겐의 작용에 의해 응고시간은 지연되었으며, 이는 neolignan glycoside가 피브린형성에 주요한 트롬빈의 활성을 직접 저해하는 것으로 사료된다.

• Journal of Photoscience
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• 제9권2호
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• pp.5-8
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• 2002

Photoperiodism is an important adaptive phenomenon in various physiological parameters including reproduction to cope with seasonal changes. Involvement of extraretinal photoreceptors in the photoperiodism in non-mammalian vertebrates has been well established. In addition, circadian clock system is known to be involved in the photoperiodic time measurement. The pathway consists of light-input system, time measurement system (circadian clock), gonadotropin releasing hormone (GnRH) production in the hypothalamus, luteinizing hormone (LH) and follicle stimulating hormone (FSH) production in the pituitary, and final gonadal development. Recently, several laboratories reported photopigments newly cloned in the pineal, eyes and deep brain in addition to already known visual pigments in the retina. These are pinopsin, parapinopsin, VA-opsin, melanopsin, etc. All these photopigments belong to the opsin family having retinal as the chromophore. However, the function of these photopigments remains unknown. I reviewed the studies on the location of the photopigments by immunocytochemistry. I also discussed the results on the action spectra for induction of gonadal development in relation with the location of the photoreceptors. Various physiologically active substances distribute in the vertebrate brain. Such substances are GnRH, GnIH, neuropeptide Y, vasoactive intestinal peptide, c-Fos, galanin, neurosteroids, etc. I summarized the immunhistochemical studies on the distribution and the photoperiodic changes of these substances and discussed the route from the deep brain photoreceptor to GnRH cells.

• 천문학회보
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• 제40권2호
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• pp.32.3-33
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• 2015

There exist scaling relations that link the mass of supermassive black holes with both the velocity dispersion and the mass of the central stellar cusp of their host galaxies. This implies that these two components grow in tandem. Feedback from actively accreting supermassive black holes (AGN), in the form of multi-phase gas outflows, has been argued to be the agent of this co-evolution. Here we employ the powerful GMOS integral field spectroscopy unit on the 8.2m Gemini-North telescope to investigate ionized gas outflows of luminous Type 2 AGN in the local Universe (z<0.1). Our sample of 6 galaxies is drawn from the Sloan Digital Sky Survey (SDSS) and was selected based on their [OIII] dust-corrected luminosity (>1042 erg/s) and signatures of outflows in the [OIII] line profile of their spatially integrated SDSS spectra. These are arguably the best candidates to explore AGN feedback in action since they are < 1% of a large local type 2 AGN SDSS sample selected based on their [OIII] kinematics. We combine a careful spectral decomposition of the [OIII] and $H{\alpha}$ line profiles with spatial information on ~0.5kpc scales to understand the outflow kinematics and energetics in these objects. We find clear evidence for strong outflows in [OIII] and occasionally $H{\alpha}$ that are clearly driven by the ionizing radiation of the AGN. We kinematically and spatially decompose outflowing and rotating ionized gas components. We find [OIII] to be a better tracer of AGN outflows, while $H{\alpha}$ appears to be strongly affected by both stellar rotation and outflows induced by ongoing star formation. The observed kinematics and spatial distribution of the ionized gas imply a large opening angle for the outflow. Finally, we find the projected outflow velocity to decrease as a function of distance, while its dispersion shows a more complex structure with a potentially initially increasing trend (out to 0.5-1kpc distances).

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1528-1528
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• 2001

Ginseng cultivated in different country or growing condition has generally different components such as saponin and protein, and it relates to efficacy and action. Protein content assumes by nitrogen content in ginseng radix. Nitrogen content could be determined by chemical analysis such as kjeldahl or extraction methods. However, these methods require long analysis time and result environmental pollution and sample damage. In this work we investigated possibility of non-destructive determination of nitrogen content in ginseng radix using near-infrared spectroscopy. Ginseng radix, root of Panax ginseng C. A. Meyer, was studied. Total 120 samples were used in this study and it was consisted of 6 sample sets, 4, 5 and 6-year-old Korea ginseng and 7, 8 and 9-year-old China ginseng, respectively. Each sample set has 20 sample. Nigrogen content was measured by electronic analysis. NIR reflectance spectra were collected over the 1100 to 2500 nm spectral region with a InfraAlyzer 500C (Bran+Luebbe, Germany) equipped with a halogen lapmp and PbS detector and data were collected every 2 nm data point intervals. The calibration models were carried out by multiple linear regression (MLR) and partial least squares (PLS) analysis using IDAS and SESAME software. Result of electronic analysis, Korean ginseng were different mean value in nitrogen content of China ginseng. Ginseng tend to generally decrease the nitrogen content according as cultivation year is over 6 years. The MLR calibration model with 8 wavelengths using IDAS software accurately predicted nitrogen contents with correlation coefficient (R) and standard error of prediction of 0.985 and 0.855%, respectively. In case of SESAME software, the MLR calibration with 9 wavelength was selected the best calibration, R and SEP were 0.972 and 0.596%, respectively. The PLSR calibration model result in 0.969 of R and 0.630 of RMSEP. This study shows the NIR spectroscopy could be applied to determine the nitrogen content in ginseng radix with high accuracy.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1125-1125
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• 2001

Micro-scale test methods are producing small-sample size where the conventional physical and chemical tests can not be used (high standard deviation, uncertain sampling conditions, low repeatability). Different small-scale test methods were developed recently for determination of physico-chemical, functional, rheological properties of wheat or wheat dough using miniaturized instruments with sophisticated sample preparation/handling and mechanics (RVA, 2 g mixograph, micro-Z-arm mixer, small-scale noodle maker, micro-baking method etc.). The small-scale methodologies can be used as basic research tools or as technology supported measurements and can be also essential in the early selection for quality traits in breeding programs. The milling as a sample preparation step is essential procedure providing good quality flour or semolina samples from small amount of grain (5-10 g) in a reproducible and reliable way. The aim of present study was to use NIR as quality control tool, and to evaluate the recently developed and manufactured micro-scale lab mill (FQC-2000) produced by Inter-Labor Co. Ltd., Hungary. The milling characteristics of the new instrument were compared to other laboratory mills and the effects of milling action on the chemical composition of fractions were analysed. The fractions were tested with both chemical and near infrared spectroscopic methods. The micro-scale milling resulted significantly different yields, particle size distributions and different fractions from compositional point of view. The near infrared spectra were sensitive enough to distinguish the fractions obtained by different milling procedures. Quantitative NIR calibration equations were developed and tested in order to measure the chemical composition of characteristic milling fractions. Special qualification procedure the PQS (Polar Qualification System) method was used for detecting the differences between fractions obtained by macro and micro-milling procedures. The results and the limitations of PQS method in this application will be discussed.

• 한국미생물·생명공학회지
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• 제10권3호
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• pp.211-215
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• 1982

Streptomyces griseorubiginosus var. soyoensis 에 의하여 두가지의 항진균성 항생물질이 생성되었는데 하나는 trans-cinnamamide 이었고 다른 하나는 UV, IR, NMR, mass spectrum 및 화학반응의 결과로 새로운 tetraene계 화합물임이 밝혀져 이 물질을 Tetraene KM-A라 하였다. 본 물질의 항균성을 조사한 결과 곰팡이 및 효모류에 대하여는 강한 항진균성을 나타내었으나 항세균성은 없었다. 배지에 sterol류를 첨가하였을때 Tetraene KM-A의 항균력이 없어지는 것으로 보아 stesoid와 결함함을 알수 있고 항균작용도 이와 관련이 있는 것으로 판단하였다. Tetraene KM-A의 mouse 및 rat에 대한 복강주사에 의한 $LD_{50}$ 은 각각 84.3과 90.4mg/kg이였으며 mouse에 대만 경구독성은 1503mg/kg이었다.

• Natural Product Sciences
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• 제25권4호
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• pp.348-353
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• 2019

Soluble epoxide hydrolases (sEH) are enzymes present in all living organisms, metabolize epoxy fatty acids to 1,2-diols. sEH in the metabolism of polyunsaturated fatty acids plays a key role in inflammation. In addition, the endogenous lipid mediators in cardiovascular disease are also broken down to diols by the action of sEH that enhanced cardiovascular protection. In this study, sEH inhibitory guided fractionation led to the isolation of five phenolic compounds trans-resveratrol (1), trans-piceatannol (2), sulfuretin (3), (+)-balanophonin (4), and cassigarol E (5) from the ethanol extract of the seeds of Passiflora edulis Sims cultivated in Vietnam. The chemical structures of isolated compounds were determined by the interpretation of NMR spectral data, mass spectra, and comparison with data from the literature. The soluble epoxide hydrolase (sEH) inhibitory activity of isolated compounds was evaluated. Among them, trans-piceatannol (2) showed the most potent inhibitory activity on sEH with an IC₅₀ value of 3.4 μM. This study marks the first time that sulfuretin (3) was isolated from Passiflora edulis as well as (+)-balanophonin (4), and cassigarol E (5) were isolated from Passiflora genus.