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Control of Sperm Penetration In Vitro by Cumulus Cells in Porcine Oocytes (돼지의 체외수정시 난구세포에 의한 정자침입의 조절)

  • Park, C.K.;Cheong, H.T.;Lee, J.H.;Kim, I.C.;Yang, B.K.;Kim, C.I.
    • Korean Journal of Animal Reproduction
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    • v.23 no.2
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    • pp.149-157
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    • 1999
  • The functional role of cumulus cells on the penetration and polyspermy during in vitro fertilization in porcine was examined. The penetration rate was significantly higher (P<0.01) in oocytes with (61%) than without (25%) cumulus cells, but significant differences in polysper-my rates were not observed. When hyaluronidase was added to the fertilization medium with different concentrations, penetration rates in oocytes with cumulus cells were higher than in oocytes without cumulus cells at 0 (61% vs 34% ; P<0.05), 0.01 (56% vs 35% ; P<0.05), 0.1 (66% vs 30% ; P<0.05) and 1.0mg/$m\ell$ (39% vs 27%). The polyspermy rates were lower in oocytes without than with cumulus cells, and had a tendency to decrease with high concentrations of hyaluronidase. In another experiment, the penetration and polyspermy rates had a tendency to increase as time of sperm-oocyte culture was prolonged. At 16 and 20 h after insemination, the penetration rates were significantly higher (P<0.05) in oocytes with (48 and 62% for 16 and 20 h) than without (25 and 31% for 16 and 20 h) cumulus cells in medium containing hyaluronidase. Polyspermy rates were significantly (P<0.05) lower in oocytes without (13% and 16%) than with (37% and 48%) cumulus cells at 16 and 20 h after insemination. In cumulus-free oocytes inseminated in medium containing different concentrations of cumulus cells, the penetration rates were significantly (P<0.05) higher in medium with than without hyaluronidase. The proportion of polyspermy was lower in medium without than with hyaluronidase at 0 (10% vs 0%), 10$^2$(25% vs 0%), 10$^4$(24% vs 14%) and 10$^{6}$ (29% vs 10% ; P<0.05) cumulus cells/$m\ell$. These results suggest that cumulus cells can have a positive influence on sperm penetration, its action on polyspermy control does appear to function primarily on zona pellucida by co-culture of cumulus cells and oocytes in medium without hyaluronidase.

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A Study on the Ethics of Reproduction in Alain Resnais's Film -Focusing on , , and (알랭 레네 영화로 본 재현의 윤리 연구 -<밤과 안개>, <히로시마 내 사랑>, <뮤리엘>을 중심으로)

  • Choi, Eun-Jeong
    • Journal of Popular Narrative
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    • v.25 no.1
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    • pp.393-425
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    • 2019
  • This paper focuses on Alain Resnais's representative works (1955), (1959), and (1963), and analyzes how he implements a representation of memory though cinematic apparatus. These three films deal with horrific memories that seem impossible to reproduce aesthetically such as the Holocaust, the Hiroshima Atomic Bomb, World War II, and the war in Algeria. The reappearance of events that stripped humans of even their minimum dignity can naturally be associated with ethical issues. These events can never be reproduced because they cannot be explained in the human language. It is also impossible to reproduce in a way that doesn't invade other peoples' sufferings, nor displays the pain of others as spectacles. Alain Resnais was a director who realized that if factual representation was not possible from the beginning, truthfulness would have to be approached through cinematic form. Therefore, he tries to overcome these problems through cinematic forms. First, he shifts to action films to avoid the obscenity of documentary. shows the records of camps captured by German forces in the past, while shows the pain of others in a fictional form of representation. Next, he describes how the trauma affects the identity of the main character through a flashback in , but also shows a main character who is experiencing trauma without a flashback in Flashbacks have the effect of showing the effects of trauma on the main character, but at the same time they involve the obscenity of enjoying the suffering of others. Nonetheless, the absence of flashbacks highlights the impossibility of representation. This is because it is not silent in the impossibility of representation but is constantly approaching. The attitude that repeatedly circles around impossibility is an ethical form that maximizes the impossibility of representation. In conclusion, this is the ethics of representation that Alain Resnais showed in his films.

A Study on the Cause and Improvement of the Red-Water Occurrence in Urban Stream (도심하천 내 적수발생 지점에 대한 원인검토 및 개선방안 연구)

  • Beomjin Eun;Jong Hwan Kim;Zi Yu Lin;Jeong Sook Heo;I Song Choi;Jong-Min Oh
    • Journal of Environmental Impact Assessment
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    • v.32 no.3
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    • pp.166-175
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    • 2023
  • This study aims to identify the cause of the red-water occurrence (the phenomenon of water being red) that occurs at some points and sections of rivers in Yongin City. As a result of conducting a preliminary investigation, total three sites were selected as the investigation point as it was found that the red-water occurrence continued. As a result of the investigation, it is judged that the cause of the red-water in Yongin-city river is due to the soil color and iron content of the region. JPS, SBS, and JJS sites all showed that the color of soil is mainly consist of reddish brown and red-yellow. The average Fe concentration was 13.75 mg/L, 10.85 mg/L, and 1.31 mg/L, for each sites, and considering that the Fe concentration in general river water was less than 0.5 mg/L, it was confirmed that the concentration was quite high. At the JPS and JJS points, the red-water occurrence occurred mainly in stagnant places, which is believed to be strengthened by the reaction of organic and microorganisms. In the case of SBS, the wateris red, but as a result of observing the actual color, it is judged that the iron component deposited in the pipe causes an optical illusion with a deep red color. In addition, it is believed that the iron concentration can be reduced to the general river water concentration range by removing the particulate iron component through a decrease of more than 95% as a result of filtering with glass fiber filter with particulate iron. As a result of this study, it is necessary to manage the river to maintain the flow, and it is believed that the occurrence of red-water at the survey point can be alleviated through uptake action through planting and agglomeration precipitation and agglomeration filtration methods for particulate iron treatment.

Effects on the continuous use intention of AI-based voice assistant services: Focusing on the interaction between trust in AI and privacy concerns (인공지능 기반 음성비서 서비스의 지속이용 의도에 미치는 영향: 인공지능에 대한 신뢰와 프라이버시 염려의 상호작용을 중심으로)

  • Jang, Changki;Heo, Deokwon;Sung, WookJoon
    • Informatization Policy
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    • v.30 no.2
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    • pp.22-45
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    • 2023
  • In research on the use of AI-based voice assistant services, problems related to the user's trust and privacy protection arising from the experience of service use are constantly being raised. The purpose of this study was to investigate empirically the effects of individual trust in AI and online privacy concerns on the continued use of AI-based voice assistants, specifically the impact of their interaction. In this study, question items were constructed based on previous studies, with an online survey conducted among 405 respondents. The effect of the user's trust in AI and privacy concerns on the adoption and continuous use intention of AI-based voice assistant services was analyzed using the Heckman selection model. As the main findings of the study, first, AI-based voice assistant service usage behavior was positively influenced by factors that promote technology acceptance, such as perceived usefulness, perceived ease of use, and social influence. Second, trust in AI had no statistically significant effect on AI-based voice assistant service usage behavior but had a positive effect on continuous use intention. Third, the privacy concern level was confirmed to have the effect of suppressing continuous use intention through interaction with trust in AI. These research results suggest the need to strengthen user experience through user opinion collection and action to improve trust in technology and alleviate users' concerns about privacy as governance for realizing digital government. When introducing artificial intelligence-based policy services, it is necessary to disclose transparently the scope of application of artificial intelligence technology through a public deliberation process, and the development of a system that can track and evaluate privacy issues ex-post and an algorithm that considers privacy protection is required.

Introduction of the Best Practices in the Pakistan Gulpur HEPP (파키스탄 Gulpur 수력발전 현장의 Best Practices 소개)

  • JANG, Ock Jae;HONG, Won Pyo;CHAE, Hee Moon
    • Proceedings of the Korea Water Resources Association Conference
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    • 2022.05a
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    • pp.216-217
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    • 2022
  • Gulpur 수력발전 프로젝트는 전력난을 겪고 있는 파키스탄에 102 MW 규모의 수력발전소를 건설하여 30년 동안 운영 관리한 후 파키스탄 정부로 양도하는 IPP(Independent Power Producing) 형식의 투자사업이다. 남동발전과 DL E&C, 롯데건설이 Sponsor로서 출자한 자본금과, ADB, IFC, K-EXIM 등의 대주단로부터의 차입금을 재원으로 하여 소요 사업비를 조달하고 사업을 개발하였다. DL E&C와 롯데건설이 EPC(Engineering, Procurement, Construction)를 수행하였고, 이산이 Design consultant의 역할을 수행하였다. Gulpur 수력발전 프로젝트의 발전형식은 수로식(run-of-river)으로 201 m3/s의 발전유량과 102 MW의 발전 시설용량을 이용하여 연평균예상발전량은 398 GWh이다. 주요 구조물로는 설계 재현빈도 1년의 유수전환시설(가물막이댐 & 가배수터널)과 콘크리트 중력식댐(H 67 m, L 205 m), 도수터널(D 6.7 m, L 215 m, 2기), 옥외형 발전소 (H 51 m, W 60 m, L 38 m, Kaplan 2기)가 있으며, 2015년 10월 착공하여 2020년 3월 상업발전을 시작하였다. 본 프로젝트는 DL E&C의 첫 번째 EPC 해외수력발전 프로젝트이다. 따라서 프로젝트의 성공적 수행을 위한 경제적 설계, 시공의 효율성 및 안정성 확보 등을 위하여 많은 연구를 수행하는 과정에서 다양한 기술 개선을 이룰 수 있었다. 본고에서는 Gulpur 프로젝트를 통하여 도출된 성공 사례들을 소개 및 공유하고자 한다. 첫 번째로 콘크리트 중력식댐 시공을 위한 유수전환시설의 최적 설계빈도를 산정하였다. 일반적으로 유수전환시설의 규모는 설계기준에 제시된 설계 재현빈도를 이용하는데, 해외 설계기준에서는 10년, 국내 설계기준에서는 1~2년으로 다르게 제시되어 있는 문제점이 있다. 유수전환시설의 규모는 프로젝트의 경제성에 큰 영향을 미치기 때문에 최적 설계빈도의 결정이 필요하며, 위험도분석기법(Risk Analysis)과 기대화폐가치법(Expected Monetary Value)을 이용하여 유수전환시설의 최적 설계 재현빈도와 이에 영향을 미치는 인자를 분석하였다. 위험도는 몬테카를로 시뮬레이션으로 산정된 가물막이댐 파괴확률과 재현빈도를 이용하여 산정된 가물막이댐 월류확률을 고려하였으며, 비용 및 피해액으로는 유수전환시설의 공사비, 가물막이댐 파괴시의 재건설비용과 지체보상금, 가물막이댐 월류시의 복구비용을 고려하였다. 이에 대한 연구결과로, 유수전환시설의 사용기간과 월류시의 복구비용이 유수전환시설의 설계 재현기간 결정에 가장 큰 영향을 미치는 것으로 나타났고, 특히 월류시의 복구비용이 작을수록 낮은 설계 재현빈도를 선택하는 것이 타당한 것으로 나타났다. 예를 들어, 유수전환시설의 사용기간이 3 ~ 5년, 복구비용이 0.5 ~ 1.0 mil USD 이하인 조건에서 가물막이시설의 최적 설계빈도는 1년 ~ 2년인 것으로 나타났다. 또한, 유수전환시설의 사용기간은 본댐의 규모와 시공기간 등을 고려하여 결정되는 사항으로 설계자가 임의 조정할 수 없지만, 복구비용은 시공 관리자에 따라 결정되는 부분으로, 적극적 홍수 피해 저감 및 복구방안을 마련하는 것이 프로젝트의 경제성을 향상시킬 수 있다는 것을 알 수 있었다. 두 번째로 프로젝트의 경제성 향상, 홍수기 댐 시공시의 안전성 확보를 위하여 홍수 조기경보시스템(Early Warning System)을 개발 및 활용하였다. 수로식(Run-of-river) 수력발전댐은 대부분 산악지역에 위치하기 때문에 국지성 강우 및 급한 지형 경사로 인하여 돌발홍수(flash flood)의 발생 가능성이 높다. 따라서 시공 중 홍수(월류) 발생을 미리 감지하고 현장에 전파할 수 있는, 수로식(Run-of-river) 수력발전댐 현장을 위한 홍수 조기경보시스템이 필요하며, 이를 리스크 인식, 모니터링 및 경보, 전파 및 연락, 반응 능력 향상의 4가지 부분으로 나누어 구축하였다. 리스크 인식 부분에서는 가물막이댐 월류 발생 상황에 대한 위험도, 취약성, 리스크를 제시하였으며, 모니터링 및 경보 부분에서는 상류 측정수위에서 유도된 현장 예상수위와 실제 현장 측정 수위를 대상으로 경보홍수위와 위험홍수위로 나누어 관리하였다. 전파 및 연락 부분에서는 현장 시공 조직을 활용하여 홍수시를 대비한 비상연락체계도(Emergency communication flow chart)를 운영하였으며, 반응 능력 향상을 위해 비상연락체계도의 팀별 Action plan을 상세화 하였다. 세 번째로 현장의 지질특성과 50여 차례 발파시험으로 현장 고유의 발파진동감쇄곡선을 도출하였으며, 이를 통해 현장의 시공성과 콘크리트 품질 확보를 동시에 달성할 수 있는 방안을 제시하였다. 콘크리트댐 공사에서는 제한된 공기 내에 공사를 완료하기 위해 사면부 굴착과 콘크리트 타설이 동시에 수행될 수밖에 없는 문제점을 가지고 있다. 그러나 신규 콘크리트 타설면 근처에서 발파를 수행하는 경우 발파로 발생되는 탄성파가 일정 수준을 초과하게 되면, 콘크리트 양생에 영향을 주게 된다. 따라서 다수의 현장 발파시험을 통해 발파거리와 최대진동속도의 상관관계 즉, 발파진동감쇄곡선을 도출함으로써 현장의 발파진동특성을 도출할 수 있었다. 또한, 기존 연구 논문들을 통해 콘크리트 재령기간 별 안전진동속도를 선정하고, 해당 안전진동속도를 초과하지 않는 범위에서 콘크리트 타설면과 발파위치의 거리에 따라 1회 발파 가능한 장약량을 산정하여 적용하였다. 이와 같은 체계적인 접근을 통해 콘크리트 타설과 발파 작업 동시 수행에 대한 논란을 해소할 수 있었다.

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A prediction model for adolescents' skipping breakfast using the CART algorithm for decision trees: 7th (2016-2018) Korea National Health and Nutrition Examination Survey (의사결정나무 CART 알고리즘을 이용한 청소년 아침결식 예측 모형: 제7기 (2016-2018년) 국민건강영양조사 자료분석)

  • Sun A Choi;Sung Suk Chung;Jeong Ok Rho
    • Journal of Nutrition and Health
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    • v.56 no.3
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    • pp.300-314
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    • 2023
  • Purpose: This study sought to predict the reasons for skipping breakfast by adolescents aged 13-18 years using the 7th Korea National Health and Nutrition Examination Survey (KNHANES). Methods: The participants included 1,024 adolescents. The data were analyzed using a complex-sample t-test, the Rao Scott χ2-test, and the classification and regression tree (CART) algorithm for decision tree analysis with SPSS v. 27.0. The participants were divided into two groups, one regularly eating breakfast and the other skipping it. Results: A total of 579 and 445 study participants were found to be breakfast consumers and breakfast skippers respectively. Breakfast consumers were significantly younger than those who skipped breakfast. In addition, breakfast consumers had a significantly higher frequency of eating dinner, had been taught about nutrition, and had a lower frequency of eating out. The breakfast skippers did so to lose weight. Children who skipped breakfast consumed less energy, carbohydrates, proteins, fats, fiber, cholesterol, vitamin C, vitamin A, calcium, vitamin B1, vitamin B2, phosphorus, sodium, iron, potassium, and niacin than those who consumed breakfast. The best predictor of skipping breakfast was identifying adolescents who sought to control their weight by not eating meals. Other participants who had low and middle-low household incomes, ate dinner 3-4 times a week, were more than 14.5 years old, and ate out once a day showed a higher frequency of skipping breakfast. Conclusion: Based on these results, nutrition education targeted at losing weight correctly and emphasizing the importance of breakfast, especially for adolescents, is required. Moreover, nutrition educators should consider designing and implementing specific action plans to encourage adolescents to improve their breakfast-eating practices by also eating dinner regularly and reducing eating out.

A qualitative study on the process of maintaining the 'eating alone'(honbob) lifestyle (직장인의 '혼밥' 유지 과정에 대한 질적 연구)

  • Hye Jin Kwon;Younga Ju
    • Korean Journal of Culture and Social Issue
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    • v.24 no.4
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    • pp.657-689
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    • 2018
  • The purpose of this study is to develop a substantive theory on 'eating alone'(honbob)and the process of maintaining the lifestyle of eating alone for the need of a non-judgmental understanding on the new 'honbob' lifestyle. Data were collected through in-depth interviews with 10 male and female workers in Seoul and Gyeonggi-do, who voluntarily eat alone over 70% of their meals per week with the minimum duration of 5 years. Data analysis was performed using grounded theory proposed by Strauss & Corbin (1998) in the qualitative research method. As a result, a paradigm model on the process of maintaining 'honbob' was derived. Based on categorical analysis, the causal condition was 'not trying to tune' and the central phenomenon was 'following the desire to set efficiency as the top priority. Contextual conditions were 'the atmosphere of fierce competition', 'weakening of organizational culture', 'diffusion of individualistic culture'. The intervening conditions were 'personal trait and emotional experience', 'job characteristics of less organization culture'. The action/interaction strategies were 'accepting internal conflicts', 'acting in autonomy', 'finding relationship through media', and 'distancing from superficial relationship'. The consequences were 'enjoying time for self-exploration', 'valuing self-care', 'becoming a epicurean conventionalist', and 'becoming aware of the need for balance'. The core category has been shown as 'self-oriented in accordance to priority of efficiency and being able to appreciate the importance of social group'. The Such phenomenon passes through four different stages - first, the stage of weighing time efficiency while beginning hon-bob; second, the stage of conflict when one feels nervous and not free from others' view; third, the stage of adjustment to justify his/her 'hon-bob'; and the final stage of balance to perceive the importance of social group while going on 'honbob'. The study had the aim of increasing the understanding and acceptance of the new 'honbob' lifestyle through an in-depth exploration of office worker's 'honbob' experience and the process of maintaining 'honbob' so the society can better accept it and, further, to embrace co-existence of various cultures.

The Exploration of New Business Areas in the Age of Economic Transformation : a Case of Korean 'Hidden Champions' (Small and Medium Niche Enterprises (경제구조 전환기에서 새로운 비즈니스 영역의 창출 : 강소기업의 성공함정과 신시장 개척)

  • Lee, Jangwoo
    • Korean small business review
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    • v.31 no.1
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    • pp.73-88
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    • 2009
  • This study examines the characteristics of 24 Korean hidden champions such as key success factors, core competences, strategic problems, and desirable future directions. The study categorized them into 8 types with Danny Miller's four trajectories and top manager's decision making style(rationality and passion). Danny Miller argued in his book, Icarus paradox, that outstanding firms will extend their orientations until they reach dangerous extremes and their momentum will result in common trajectories of decline. He suggested four very common success types: Craftsmen, Builders, Pioneers, Salesmen. He also suggested common trajectories of decline:Focusing(from Craftsmen to Tinkers), Venturing(from Builders to Imperialists), Inventing(from Pioneers to Escapists), Decoupling(from Salesmen to Drifts). In Korea, successful startups appear to possess three kinds of drive: Technology-drive, Vision-drive, Market-drive. Successful technology-driven firms tend to grow as craftsmen or pioneers. Successful vision-driven and market-driven ones tend to grow as builders and salesmen respectively. Korean top managers or founders seem to have two kinds of decision making style: Passion-based and Rationality-bases. Passion-based(passionate) entrepreneurs are biased towards action or proactiveness in competing and getting things done. Rationality- based ones tend to emphasis the effort devoted to scanning and analysing information to better understand a company's threats, opportunities and options. Consequently this study suggested 4*2 types of Korean hidden champions: (1) passionate craftsmen, (2) rational craftsmen, (3) passionate builders, (4) rational builders, (5) passionate pioneers, (6) rational pioneers, (7) passionate salesmen, (8) rational salesmen. These 8 type firms showed different success stories and appeared to possess different trajectories of decline. These hidden champions have acquired competitive advantage within domestic or globally niche markets in spite of the weak market power and lack of internal resources. They have maintained their sustainable competitiveness by utilizing three types of growth strategy; (1) penetrating into the global market, (2) exploring new service market, (3) occupying the domestic market. According to the types of growth strategy, these firms showed different financial outcomes and possessed different issues for maintaining their competitiveness. This study found that Korean hidden champions were facing serious challenges from the transforming economic structure these days and possessed the decline potential from their success momentum or self-complacence. It argues that they need to take a new growth engine not to decline in the turbulent environment. It also discusses how firms overcome the economic crisis and find a new business area in promising industries for the future. It summarized the recent policy of Korean government called as "Green Growth" and discussed how small firms utilize such benefits and supports from the government. Other implications for firm strategies and governmental policies were discussed.

Comparison of Carbon Storage Based on Alternative Action by Land Use Planning (토지이용에 따른 대안별 탄소 저장량 비교)

  • Seulki Koo;Youngsoo Lee;Sangdon Lee
    • Journal of Environmental Impact Assessment
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    • v.32 no.6
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    • pp.377-388
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    • 2023
  • Carbon management is emerging as an important factor for global warming control, and land use change is considered one of the causes. To quantify the changes in carbon stocks due to development, this study attempted to calculate carbon storage by borrowing the formula of the InVEST Carbon Storage and Sequestration Model (InVEST Model). Before analyzing carbon stocks, a carbon pool was compiled based on previous studies in Korea. Then, we estimated the change in carbon stocks according to the development of Osong National Industrial Park (ONIP) and the application of alternatives. The analysis shows that 16,789.5 MgC will be emitted under Alternative 1 and 16,305.3 MgC under Alternative 2. These emissions account for 44.4% and 43.1% of the pre-project carbon stock, respectively, and shows that choosing Alternative 2 is advantageous for reducing carbon emissions. The difference is likely due to the difference in grassland area between Alternatives 1 and 2. Even if Alternative 2 is selected, efforts are needed to increase the carbon storage effect by managing the appropriate level of green cover in the grassland, creating multi-layered vegetation, and installing low-energy facilities. In addition, it is suggested to conserve wetlands that can be lost during the stream improvement process or to create artificial wetlands to increase carbon storage. The assessment of carbon storage using carbon pools by land cover can improve the objectivity of comparison and evaluation analysis results for land use plans in Environmental Impact Assessment and Strategic Environmental Impact Assessment. In addition, the carbon pool generated in this study is expected to be used as a basis for improving the accuracy of such analyses.

Application of the Extract of Zanthoxylum piperitum DC to Manufacturing Eco-friendly Nosocomial Infection Control Protective Materials (초피의 항균 활성을 이용한 원내 감염 제어 친환경 방호 소재 개발)

  • Shin Young Park;Ki Yun Kim;Do Youn Jun;Sung Chul Kim;Hyo-Il Jung;Young Ho Kim
    • Journal of Life Science
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    • v.33 no.10
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    • pp.820-827
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    • 2023
  • Since COVID-19 began at the end of 2019, the wearing time of protective clothing used to prevent pathogenic bacteria and virus infection has increased, and the development of safe protective materials that are human-friendly and have antibacterial and antiviral functions has been required. In this study, we investigated the possibility of developing natural antibacterial protection materials using ethanol extract of the medicinal plant Zanthoxylum Piperitum DC. The antibacterial activity assay of the 80% ethanol extract of Z. piperitum DC leaves against various nosocomial infectious bacteria, using the disk diffusion method, showed that Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae ATCC 13883, Salmonella typhimurium, and Aeromonas hydrophila are sensitive to the inhibitory action of the extract. The IC50 values of the ethanol extract against S. aureus, K. pneumoniae, P. vulgaris and A. hydrophila were about 0.59 mg/ml, 0.50 mg/ml, 1.06 mg/ml, and 0.06 mg/ml, respectively. To determine whether the ethanol extract of Z. piperitum DC leaves can be applied to the development of antibacterial protective fabric, the ethanol extract was tested using a protective fabric from the KM Health Care Corp. using the JIS L1902-Absorption method. As a result, the bacteriostatic and bactericidal activity values of S. aureus ATCC 25923 and K. pneumoniae ATCC 13883 appeared to be more than 2.0 when treated with the ethanol extract at a concentration of 1% (w/v). Together, these results suggest that Z. piperitum DC leaves can be applied to develop natural antibacterial functional protective fabrics.