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Effects of Total Mixed Rations with Corn on Growth and Meat Quality of Castrated Korean Black Goats (옥수수 첨가 TMR 사료 급여가 거세흑염소의 발육과 육질에 미치는 영향)

  • Kim, Sang-Uk;Choi, Young-Sun;Yoo, Dae-Joong;Ku, Min-Jung;Lee, Gi-Ho;Park, Sang-Guk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.4
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    • pp.350-356
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    • 2016
  • The objective of this study was to evaluate the effect of different dietary corn levels in Total Mixed Rations (TMR) on growth and meat quality of castrated Korean black goats. For feeding trials, thirty-two castrated Korean black goats with similar age (average of 3 months) and body weight (average of 15.9 kg) were divided into the following four treatment groups: commercial feed (control), TMR containing 15% of corn (T1), TMR containing 25% of corn (T2), and TMR containing 35% of corn (T3) in a randomized complete block design. Final body weight was the highest in T2 (58.9 kg), followed by that in control group (53.1 kg). Average daily gain (ADG) and total dry matter intake (TDMI) were also the highest in T2 (100.2 g and 1,346 g, respectively). However, feed conversion ratio (13.4) was the lowest in T2. Carcass weight and dressing percentage in T2 and T3 were higher than those in the control. For meat quality characteristics, crude fat content was the highest in T2 (6.54%). Cooking loss (15.2%) was the lowest in the control. However, the control had the highest flavor value (5.3%). Oleic acid (C18:l) and palmitic acid (C16:0) were the highest in T2 (48.6% and 22.4%, respectively). Stearic acid (C18:0) was the highest in T1 (14.5%). Unsaturated fatty acid was the highest in the control (58.8%), followed by that in T3 (58.2%), T2 (57.8%), and T1 (54.4%). These results indicate that TMR containing 25% of corn could improve the growth performance and meat quality of castrated Korean black goats.

Effect of water extract and distillate from the mixture of black goat meat and medicinal herb on osteoblast proliferation and osteoclast formation (흑염소와 약용식물 복합 증탕추출액 및 증류액이 조골세포 증식과 파골세포 형성에 미치는 영향)

  • Song, Hyo-Nam;Leem, Kang-Hyun;Kwun, In-Sook
    • Journal of Nutrition and Health
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    • v.48 no.2
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    • pp.157-166
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    • 2015
  • Purpose: The effects of water extract and distillate from the mixture of black goat meat and medicinal herb on MG-63 osteoblast proliferation and mouse bone marrow derived osteoclast formation were investigated. Methods: Proximate composition, volatile basic nitrogen (VBN), mineral content, free amino acid composition and free fatty acid composition in black goat meat were determined. Water extract and distillate were prepared with three groups; goat meat only (BG-E, BG-D), six herbs added group (BG-E6, BG-D6), and eight herbs added group (BG-E8, BG-D8). Osteoblast proliferation, mineralization and calcium uptake activity of MG-63 cells were measured and tartrate resistant acid phosphatase activity of osteoclasts was analyzed. Results: Black goat meat had remarkably low fat and high level of calcium. Glutamic acid was the most abundant amino acid. Herbs added extract groups (BG-E6 and BG-E8) showed increased MG-63 cell proliferation in a concentration dependent manner, while all the distillates did not show the effect. All extracts and distillates showed significantly increased osteoblast mineralization depending on the concentration. In particular, herb added extract, BG-E6, increased 170.3% of control and the distillate of BG-D and BG-D6 increased up to 168.5% and 159.8%, respectively. Calcium uptake activities of all water extracts showed remarkable increase of BG-E6 and BG-E8 up to 615.5% and 628.1% of control, respectively. Ditillates had no effect except BG-D6. All water extracts significantly reduced the activity of tartrate-resistant acid phosphatase (TRAP) in osteoclasts derived from mouse bone marrow. Conclusion: Combination of black goat meat and medicinal herb increased the MG-63 cell proliferation and effectively inhibited osteoclast differentiation in both water extracts and distillate of them, which implies that they could be used as potent functional food materials for bone health.

Development of Black Garlic Yakju and Its Antioxidant Activity (흑마늘 발효주 개발 및 항산화 활성)

  • Lee, Hyo-Hyung;Kim, Ig-Jo;Kang, Sang-Tae;Kim, Yeong-Hoon;Lee, Jeong-Ok;Ryu, Chung-Ho
    • Korean Journal of Food Science and Technology
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    • v.42 no.1
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    • pp.69-74
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    • 2010
  • Black garlic has recently received significant attention due to its various health functional properties, and there has been an increase in demand for its use as a functional food. This study was performed to determine the optimum concentration for the fermentation of black garlic yakju. In addition, the antioxidant activity of the fermented black garlic yakju was examined. The alcohol content in the black garlic yakju significantly increased for 6 days and the pH gradually increased as the concentration of black garlic increased. The reducing sugar content at each black garlic concentration was maximal when it was fermented for 24 hours, and then rapidly decreased at longer fermentation periods. The main organic acids were lactic, citric, malic and oxalic acid. Also, the lactic acid content increased as the concentration of the black garlic increased where as the content of other organic acids decreased. The total polyphenol content, ferric ion reducing antioxidant power (FRAP) activity and DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical scavenging activity of black garlic yakju increased as the concentration of black garlic increased. The sensory characteristics of fermented black garlic yakju were evaluated in terms of color, flavor, taste and overall acceptability, and the highest overall acceptability value was obtained for yakju containing a black garlic concentration of 1-3%. Therefore, the optimum concentration of black garlic was determined to be 1% for the production of high quality black garlic yakju.

Phenolic compounds and antioxidant activity of berries produced in South Korea

  • Lee, Yongcheol;Lee, Jea-Kyoo;Kim, Jeong-Gon;Park, So-Hyun;Kim, Young-Eun;Park, Sung-Kyu;Kim, Moo-Sang
    • Journal of Applied Biological Chemistry
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    • v.63 no.4
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    • pp.297-303
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    • 2020
  • Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities were investigated for comparison of the biological activity of the berry extracts. Chlorogenic acid was the major phenolic acid, which had the highest content in aronia, followed by blueberry. The rutin and isoquercetin contents were the highest in mulberry, followed by aronia, black raspberry, and blueberry. The anthocyanin content was the highest in black raspberry. Aronia showed the highest DPPH free radical and nitrite scavenging activities with the highest contents of total phenolics and proanthocyanidins.

Cloning and characterization of a cDNA encoding a paired box protein, PAX7, from black sea bream, Acanthopagrus schlegelii

  • Choi, Jae Hoon;Han, Dan Hee;Gong, Seung Pyo
    • Journal of Animal Reproduction and Biotechnology
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    • v.36 no.4
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    • pp.314-322
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    • 2021
  • Paired box protein, PAX7, is a key molecule for the specification, maintenance and skeletal muscle regeneration of muscle satellite cells. In this study, we identified and characterized the cDNA and amino acid sequences of PAX7 from black sea bream (Acanthopagrus schlegelii) via molecular cloning and sequence analysis. A. schlegelii PAX7 cDNA was comprised of 1,524 bp encoding 507 amino acids and multiple sequence alignment analysis of the translated amino acids showed that it contained three domains including paired DNA-binding domain, homeobox domain and OAR domain which were well conserved across various animal species investigated. Pairwise Sequence Alignment indicated that A. schlegelii PAX7 had the same amino acid sequences with that of yellowfin seabream (A. latus) and 99.8% identity and similarity with that of gilt-head bream (Sparus aurata). Molecular phylogenetic analysis confirmed that A. schlegelii PAX7 formed a monophyletic group with those of teleost and most closely related with those of the fish that belong to Sparidae family including A. latus and S. aurata. In the investigation of its tissue specific mRNA expression, the expression was specifically identified in skeletal muscle tissue and a weak expression was also shown in gonad tissue. The cultured cells derived from skeletal muscle tissues expressed PAX7 mRNA at early passage but the expression was not observed after several times of subculture.

Optimal HPLC Condition for Simultaneous Determination of Anthocyanins in Black Soybean Seed Coats (검정콩 함유 안토시아닌의 동시분석을 위한 최적 HPLC 분석 조건)

  • Choung, Myoung-Gun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.4
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    • pp.359-368
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    • 2008
  • Black soybean has been widely utilized as foods and oriental medicinal materials. The pigmentation in the seed coat of black soybean is due to accumulate anthocyanins in the epidermis palisade layer. The anthocyanins of black soybean seed coat are considered as a parameter of quality evaluation of black soybean. Therefore, the purpose of this study was to investigate the most suitable HPLC condition for simultaneous determination of anthocyanins in black soybean seed coats extracts. The efficient HPLC analytical condition of D3G, C3G, and Pt3G contained extracts of black soybean seed coats was developed. The gradient elution employed a $250\;mm\;{\times}\;4.6\;mm$ i.d. YMC-pak ODS-AM 303 column. The gradient system was used two mobile phases. A gradient elution was performed with mobile phase A, consisting of 5% aqueous formic acid, and mobile phase B, comprising 5% formic acid - acetonitrile, and delivered at a flow rate of 0.7 mL/min as follows: $0{\sim}35\;min$, $90%\;A{\sim}60%\;A$; 36 min, 90% A; 46 min, 90% A. The UV-VIS. detection wavelength was set at 520 nm. The limit of detection (LOD) for D3G, C3G, and Pt3G were under 10 ng/mL.

Antioxidant capacity of anthocyanin-rich fruits and vegetables and changes of quality characteristics of black carrot added pudding according to storage (안토시아닌 함유 과채소류의 생리활성성분 함량과 항산화능 및 저장에 따른 자색당근 추출물 첨가 푸딩의 품질특성 비교)

  • Kang, Suna;Lee, Soo Hyun;Shim, Young Nam;Oh, Min Ji;Lee, Na Ra;Park, Sunmin
    • Journal of Applied Biological Chemistry
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    • v.59 no.4
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    • pp.273-280
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    • 2016
  • We investigated the contents of total phenols, anthocyanins, carotenoids and the antioxidant capacity of black carrot, black currant, acaiberry, black raspberry, and blueberry. We also examined the physical and organoleptic characteristics of pudding with added black carrot extract following 7 days of storage. Black carrot and black raspberry had the highest total phenols. Blueberry contained the highest anthocyanins and black currant equaled black carrot in carotenoids. Anti-oxidant capacity measured by 1,1-diphenyl-1,2-picrylhydrazyl and 2,2-azino-bis-3-ethyl-benthiazolin-6-sulfonic acid was highest in black raspberry and black currant followed closely by black carrot. In pudding, redness was increased and blueness decreased by adding black carrot extract, but the trend was partially reversed during 7-day storage due to oxidation of the anthocyanins, which are stable in acid situation. As black carrot extract contents were increased, pH value decreased. The pudding's hardness, adhesiveness, springiness, cohesiveness, gumminess and chewiness were optimal with 7.5 % black carrot extract. In conclusion, black carrot is moderately rich in anthocyanins and phenolic compounds. In pudding adding 7.5 % black carrot extract was optimal for organoleptic qualities. However, additional studies are needed to develop methods for protecting anthocyanins from breaking-down during storage of back carrot pudding.

Chemical Components of Black Soybean Seeds Collected in Korea (수집 재래 검정콩의 화학적 성분)

  • Kim, Seok-Dong;Kim, Yong-Ho;Hong, Eun-Hi;Lee, Hong-Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.1
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    • pp.1-7
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    • 1993
  • The 1,081 soybean genotypes including 972 black soybeans and 109 other colored soybeans were collected in 197 locations from January to April, 1991. Seed chemical components of soybean collections in relation to growth characters were evaluated to survey germplasms for black soybean breeding for seed quality. Seeds of 1,081 genotypes collected were analyzed for crude protein, crude lipid, and total sugar contents. The crude protein content was averaged to 39.8% and ranged from 34.1% to 48.0%. The average crude lipid and total sugar contents were 20.1% and 10.1%, and the ranges of those were 14.1% to 23.8% and 8.3% to 12.1%, respectively. Higher crude protein content was shown in early soybean maturity group, whereas higher total sugar content was in late group. Black soybean collections with green seed embryo tended to be higher in total sugar content than those with yellow one. Black soybean oil is generally composed of about 22% oleic, 55% linoleic, 7% linolenic acid, and 16% the others.

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The Research on the Differences & Changes in Hair Color Before v.s. After shampoo and Dry on Different Heat Processes When Acid Hair Color Dyeing (산성컬러 염색제로 모발 염색 시 열처리에 따른 세척 전과 세척 건조 후 색차 및 색변화에 관한 연구)

  • An, Hyeon-Kyeong
    • Journal of Fashion Business
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    • v.16 no.1
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    • pp.69-82
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    • 2012
  • This thesis aimed to reduce the differences of hair color when hair coloring, so it researched the differences & changes in hair color before shampoo v.s. after shampoo and dry on different heat processes when acid hair color dyeing. Five hair color dyes (Y, R, B, G, Br) manufactured by two different corporations were used. The acid hair color dyes were tinted on black and bleached hair pieces subjected to 3 different heat process; 1. Normal Temperature($25^{\circ}C$, 30min.) / 2. Heating($40^{\circ}C$, 15min.)+Normal Temperature($25^{\circ}C$, 15min.) / 3. Heating($40^{\circ}C$, 30min.). Color numbers were divided by NCS value, chroma, and hue. Statistical averages were derived and t-test was conducted using SPSS V12. Hair color differences and changes were drawn on an NCS chart using Photo Shop PS. The conclu is; If acid hair colorings are separated by a heating process, hair value & chroma change before shampoo vs. after shampoo & dry regardless of the color of hair and the heat process. Hue is not changed or shifted counter clockwise NCS color circle, but some exceptions, and it's the same when the total heat process results are combined. Black hair's value shifted downward and chroma left, and hue stayed either neutral or one color or it shifted counter clockwise on NCS color circle. Bleached hair's value shifted upward and chroma right, and hue stayed one color or shifted counter clockwise, but some exceptions. And it can be shown on NCS chart.

Synthesis of 4,5-Diphenyl Imidazolone and Studies on its Fluorescent Effect (4,5-Diphenyl-Imidazolone의 合成 及 螢光效果에 關한 硏究)

  • Jeon, Poong-Jin;Kim, Hyung-Sook
    • Journal of the Korean Chemical Society
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    • v.4 no.1
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    • pp.70-77
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    • 1957
  • 4,5-Diphenyl Imidazolone is synthesized from Benzoin, Urea, and Acetic acid catalyser. Nowadays, it is being used as an optical bleaching agent for wool and nylon textiles. Up to now, only one process of synthesis has been known. In order to find out the best conditions governing the yield were examined under various catalysers and conditions. In this experiment, the summary of results were as follows. a. On Acetic acid catalyser. The maximum yield conditions were mol ratio (Benzoin: Urea: Acetic acid) 1 : 2 : 14, Acetic acid concentration 99.9%. Reaction temperature 115$^{\circ}$. Under reaction time of 2 hours, above yield was 96.4%. b. On Mineral acid Catalyser. In using of Sulfonic acid, the color of solution was changed dark purlish black. With other mineral acid catalysers, in spite of increasing of temperature, it was proved that Benzoin floats on the solution, so that this reaction could not be continue. c. On Phosphoric acid catalyser. It was made clear that it can not be used for this reaction. d. On Sodium hydroxide catalyser. As one of Alkali catalyser, Sodium hydroxide was examined but this was unsuitable substance for this reaction. e. On Formic acid catalysers. The maximum yield conditions were mol ratio (Benzoin: Urea: Formic acid) 1: 2: 30. Formic acid concentration 85.%. Reaction temperature 150∼110$^{\circ}$. Under reaction time of 90 minutes, the best yield was 87%. Hereby, it was proved that organic acids such as Acetic acid and Formic acid can be used. When using Acetic acid, the yield was better than Formic acid, but it takes longer reaction time than Formic acid. About the fluorescent effect, the temperature of dye-bath must not be over 904,5-Diphenyl Imidazolone is synthesized from Benzoin, Urea, and Acetic acid catalyser. Nowadays, it is being used as an optical bleaching agent for wool and nylon textiles. Up to now, only one process of synthesis has been known. In order to find out the best conditions governing the yield were examined under various catalysers and conditions. In this experiment, the summary of results were as follows. a. On Acetic acid catalyser. The maximum yield conditions were mol ratio (Benzoin: Urea: Acetic acid) 1 : 2 : 14, Acetic acid concentration 99.9%. Reaction temperature 115$^{\circ}$. Under reaction time of 2 hours, above yield was 96.4%. b. On Mineral acid Catalyser. In using of Sulfonic acid, the color of solution was changed dark purlish black. With other mineral acid catalysers, in spite of increasing of temperature, it was proved that Benzoin floats on the solution, so that this reaction could not be continue. c. On Phosphoric acid catalyser. It was made clear that it can not be used for this reaction. d. On Sodium hydroxide catalyser. As one of Alkali catalyser, Sodium hydroxide was examined but this was unsuitable substance for this reaction. e. On Formic acid catalysers. The maximum yield conditions were mol ratio (Benzoin: Urea: Formic acid) 1: 2: 30. Formic acid concentration 85.%. Reaction temperature 150∼110$^{\circ}$. Under reaction time of 90 minutes, the best yield was 87%. Hereby, it was proved that organic acids such as Acetic acid and Formic acid can be used. When using Acetic acid, the yield was better than Formic acid, but it takes longer reaction time than Formic acid. About the fluorescent effect, the temperature of dye-bath must not be over 90$^{\circ}$. and the ratio of 4,5-Diphenyl Imidazolone and water should be from 1:50000. to 1:10000. It proved that the best effect on textiles, and the best condition were dye-temperature near 704,5-Diphenyl Imidazolone is synthesized from Benzoin, Urea, and Acetic acid catalyser. Nowadays, it is being used as an optical bleaching agent for wool and nylon textiles. Up to now, only one process of synthesis has been known. In order to find out the best conditions governing the yield were examined under various catalysers and conditions. In this experiment, the summary of results were as follows. a. On Acetic acid catalyser. The maximum yield conditions were mol ratio (Benzoin: Urea: Acetic acid) 1 : 2 : 14, Acetic acid concentration 99.9%. Reaction temperature 115$^{\circ}C$. . Under reaction time of 2 hours, above yield was 96.4%. b. On Mineral acid Catalyser. In using of Sulfonic acid, the color of solution was changed dark purlish black. With other mineral acid catalysers, in spite of increasing of temperature, it was proved that Benzoin floats on the solution, so that this reaction could not be continue. c. On Phosphoric acid catalyser. It was made clear that it can not be used for this reaction. d. On Sodium hydroxide catalyser. As one of Alkali catalyser, Sodium hydroxide was examined but this was unsuitable substance for this reaction. e. On Formic acid catalysers. The maximum yield conditions were mol ratio (Benzoin: Urea: Formic acid) 1: 2: 30. Formic acid concentration 85%. Reaction temperature 150∼110$^{\circ}C$. Under reaction time of 90 minutes, the best yield was 87%. Hereby, it was proved that organic acids such as Acetic acid and Formic acid can be used. When using Acetic acid, the yield was better than Formic acid, but it takes longer reaction time than Formic acid. About the fluorescent effect, the temperature of dye-bath must not be over 90$^{\circ}C$. and the ratio of 4,5-Diphenyl Imidazolone and water should be from 1:50000. to 1:10000. It proved that the best effect on textiles, and the best condition were dye-temperature near 70$^{\circ}C$. and dye-time 15 minutes. . and dye-time 15 minutes. . and the ratio of 4,5-Diphenyl Imidazolone and water should be from 1:50000. to 1:10000. It proved that the best effect on textiles, and the best condition were dye-temperature near 70$^{\circ}C$. and dye-time 15 minutes.

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