• Microbiology and Biotechnology Letters
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• v.23 no.5
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• pp.588-592
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• 1995

Fed-batch fermentation was used to produce the high concentrations of poly-$\beta $-hydroxybutyrate (PHB) and poly-$\beta $-(hydroxybutyrate-co-hydroxyvalerate) (PHB/V). Specific growth rate ($\mu $), yield of cell from glucose (Y$_{x/s}$) were calculated from the two samples in 3 to 5 hours of interval and they were reflected on the determination of glucose feeding rate to maintain the glucose concentration at around 10 g/l in the culture broth. PHB was accumulated after the nitrogen became limited at 60 g/l of dry cell weight by changing ammonia water to 4N-NaOH solution. As results, the final dry cell weight (DCW) of 170 g/l, PHB of 115 g/l were obtained in 50 hours and the overall productivity was 2.4 g/l$\cdot $h. After PHB accumulation, cosubstrate of glucose and propionic acid (PA) was fed to accumulate PHB/V. But, PA feeding rate was decreased from 3 g/l$\cdot $h to 1 g/l$\cdot $h to prevent PA from accumulating to high level in the broth, which is very inhibitory to the cells. As results, DCW, PHB and PHV were 147.5 g/l, 90 g/l and 8 mole % of hydroxyvalerate, respectively.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.590-600
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• 2003

The matrix metalloproteinases (MMPs) are a family of enzymes responsible for degrading connective tissue. MMPs catalyze the breakdown of collagen from the extracellular matrix, leading to wrinkle formation and accelerated skin aging. Furthermore, ultraviolet irradiation causes increased expression of certain MMPs. In the extracellular matrix turnover, MMPs are interacting with endogenous regulators named tissue inhibitors of metalloproteinases (TIMPs). Using peptide substrate assays, it has been demonstrated that TIMP-MMP complexes interact highly specifically with $K_{i}$ values of 10$^{-9}$ -10$^{-16}$ M. Therefore applications for TIMP as inhibitor of collagen degradation are suggested for cosmetic anti-aging products to prevent wrinkle formation and loss of elasticity. To date four TIMP proteins (TIMP-1, TIMP-2, TIMP-3 and TIMP-4) have been identified which show a high degree in sequence similarity. The production of human TIMP-2, a 194-residue nonglycosylated protein, was performed by fed-batch culture of Escherichia coli. TIMP-2 accumulated in the bacterial cells in an insoluble form as inclusion bodies. The inclusion bodies were solubilized and the protein refolded to yield the native TIMP-2 in the active form. The integrity of the protein was confirmed by mass analysis, Edman sequencing and gel shift experiments with authentic samples. The inhibitory activity of the refolded and purified TIMP-2 was demonstrated with MMP-1 and MMP-2 assays using synthetic fluorogenic peptide substrates.s.

• Journal of Korean Society of Water and Wastewater
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• v.20 no.3
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• pp.329-338
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• 2006

The research aims to provide the basic data for practical applications by correlating the bio-kinetic coefficients with the load of recalcitrant organic matter in box-mill wastewater. The activated sludge process was employed to a Wastewater disposal plant in an industrial setting, increase of consequently leading to the organic load. The parameter values derived by Monod-kinetic analysis were as follows:specific substrate removal rate $K_{max}=0.17day^{-1}$, half saturation constants $K_s=60.37mg/l$, decay coefficient $K_d=0.142day^{-1}$, microbial yield coefficient y = 0.388mg/mg, and max specific growth rate ${\mu}_{max}=0.006day^{-1}$. In view of biodegradability, the $TCOD_{Mn}/TBOD_5$ ratios of inflow and outflow were 1.07 and 1.41, and the $SCOD_{Mn}/SBOD_5$ ratios of inflow and outflow were 1.10 and 1.50, respectively. The higher $TCOD_{Mn}/TBOD_5$ ratio of outflow indicated that metabolites of a microorganism have accumulated in the cells.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.15 no.2
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• pp.197-207
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• 2002

Energy dissipation capacity and earthquake responses of steel structures installed with unbonded braces(UB) were investigated. Parametric studies were performed for a single-degree-of-freedom structure under harmonic loads, and optimum yield strength of unbonded braces were derived. Nonlinear dynamic time history analyses were carried out to investigate the seismic response of multi-story model structures with UB having various size and strength. Various techniques were applied to determine proper story-wise distribution of UB in multi-story structures. The analysis results show that the maximum displacements of structures generally decrease as the stiffness of UB increases. However for some natural frequencies and seismic loads the maximum displacement and accumulated damage increases as the stiffness of UB increases.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.61 no.11
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• pp.1641-1645
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• 2012

In this study, the ZnO nanorod is grown on the seed layered glass substrate by applying an external electric field to fabricate the ZnO nanorod with the high quality and to increase the yield of the ZnO nanorod. It is possible to grow the definite and clear hexagonal ZnO nanorod as the cathode of the high voltage is connected to the side of the seed layered glass substrate and the anode is connected to the opposite side because more $Zn^{2+}$ ions are located around the ZnO seed layer and are accumulated easily due to the external electric field. As a result, it is succeeded to fabricate the definite hexagonal ZnO nanorod having better structural characteristics by applying the external electric field during the growth process. Therefore, it is demonstrated that the external electric field is effective to fabricate the high quality ZnO nanorod without changing any composition of the ZnO nanorod.

• KSBB Journal
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• v.6 no.1
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• pp.35-43
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• 1991

The production of poly-$\beta$-hydroxybutyrate(PHB) from methanol by batch and fed-batch cultivations of Methylobacterium sp. GL-10 was studied. PHB accumulation was stimulated by the nutrients deficiency including, NH4+, SO42-, and K+. The nitrogen deficiency was the most critical factor for PHB accumulation. In batch cultivation, the maximum cell concentration and PHB content were 1.86g/l and 0.62g/l, respectively, with 1.0%(v/v) of methanol and 0.5g/1 of ammonium sulfate. The mass doubling time of Methylobacterum sp. GL-10 was in the range of 4-5 hrs. The cell growth and PHB accumulation were severely inhibited at the methanol concentration over than 2% (v/v). To overcome methanol Inhibition, constant feeding and intermittent feedillg fed-batch cultivations were adopted, using C/N molar ratio as a control factor. In constant feeding fed-batch process, cell concentration was increased up to 2.67g/1, and PHB yield was enhanced from 0.33 of batch culture to 0.53. The relatively low cell concentration was caused by methanol accumulated in culture broth at late growth phase. To prevent methanol accumulation and to maximize PHB production, DO-state intermittent fed-batch cultivation was attempted. The cell and PHB concentration was reached up to 4.55g/1 and 1.80g/1, respectively. It was possible to maintain methanol concentration low and also to feed nutrient of desired C/N molar ratio.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.1
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• pp.44-48
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• 1999

Uptake, assimilation and translocation of nitrogen and dry matter assimilation and translocation in ten rice cultivars were observed in no-till direct-sown rice-vetch cropping system. There was a large degree of variation in N-uptake, grain yield, nitrogen translocation efficiency and dry matter assimilation and translocation in tested rice cultivars. Forty kg N/ha base, as compound fertilizer (21-17-21% of N-P-K) three weeks after sowing and 30 kg N/ha top-dressed at panicle initiation stage as in the form of (NH$_4$)$_2$$CO_2$ was applied. ‘Newbounet’, ‘Daesanbyeo’, and ‘Hwayeongbyeo’ showed higher translocation efficiency. The contribution of pre-heading dry matter assimilates to grain ranged from 33% to 99% of dry grain weight. Dry matter of ‘Calrose 76’ was lower than Newbounet but N content was higher in Calrose 76 than Newbonnet. By maturity, N content in vegetative parts declined considerably more than dry matter, vegetative and reproductive parts, N translocation efficiency, and N harvest index. Nitrogen translocation efficiency was greater in ‘Nonganbyeo’, Daesanbyeo, and Newbounet. Grain N concentration was positively correlated with N concentration or N content of the vegetative parts at heading in Nonganbyeo, ‘Dasanbyeo’, ‘Dongjinbyeo’, and Newbonnet. These results indicated that the greater amount of dry matter and N accumulated before heading stage, the higher translocation rates of dry matter to grain and the greater net losses at maturity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.3
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• pp.223-229
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• 1994

To find out the effects of phosphatic fertilizer application levels on the botanical composition, mineral contents and mineral nutrient deprivation of grasses and the chemical properties of soil, this expriment was arranged as a randomized complete block design with six treatments(0, 50, 100, 150, 200 and 250kg $P_2O_5$/ha) and conducted at hilly land in Gwangju, Gyeonggi Province from September, 1989 to October, 1992. The results obtained are summarized as follows: The botanical composition of grasses without posphatic fertilizer application was poor but with 150kg $P_2O_5$/ha application, remarkably increased in legume of grasses and decreaed in weed. As the phophatic fertilizer levels increase, P, Ca and Mg content of grasses were increased but N and K content tended not to be regular, and Ca/p and K/Ca+ Mg equivalent ratio were lowered. Up to 150kg $P_2O_5$/ha application level, the mineral nutrient content deprived by dry matter yield was increased but with that over application, tend not to be increased. Most of applied mineral elements were accumulated at a subsurface of grassland and as the phosphatic fertilizer application levels increase, the available $P_2O_5$ and exchangeable Mg contents of the soil tended to be remarkably increased and exchangeable K content tended to be decreased, but pH, OM and exchangeable Ca content tended not to be regular.

• Journal of Plant Biology
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• v.22 no.1_2
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• pp.21-27
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• 1979

Sandy loam plots of pH 5.5, which was created by the reclamation of highlands in Yongin county, Kyeonggi province, was selected for the present experimental study. Sugar beet was cultured in these plots by treating them with calcium hydroxide and 3 replications to maintain pH 5.5, 6.4, 6.8, 7.2, and 7.6, respectively. The growth and yield of sugar beet were investigated and the results obtained showed a great deal of boron deficiency. The growth in its early stage was favorable in high pH plots, while no significant difference was observed in sugar content, root weight and sugar production at the time of harvest in each treated plot. The tendency of sugar accumulation appeared to be identical in terms of pattern in each treated plot; i.e. sugar was accumulated until early September but declined momentarily from the middle of September and then the sugar accumulation was resumed from the middle of October. The number of individuals in boron deficiency was larger in high pH plots and the condition of such boron deficiency was also severe. The result of analysis of boron contained in plant body revealed that the boron content of individuals caused boron deficiency by water culture and that occurred in the plots was small alike. The appearance of boron deficiency is characterized by the emergence of black stripes on the surface of the basal part of petiole, severe necrosis of young leaves in the area of growing point and severe wrinkles. Therefore it is felt necessary to pay particular attention to boron deficiency in the culture of sugar beet in our country.

• The Journal of Korea Robotics Society
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• v.9 no.1
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• pp.28-38
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• 2014

This paper verifies the performance of Extended Kalman Filter(EKF) and MCL(Monte Carlo Localization) approach to localization of an underwater vehicle through experiments. Especially, the experiments use acoustic range sensor whose measurement accuracy and uncertainty is not yet proved. Along with localization, the experiment also discloses the uncertainty features of the range measurement such as bias and variance. The proposed localization method rejects outlier range data and the experiment shows that outlier rejection improves localization performance. It is as expected that the proposed method doesn't yield as precise location as those methods which use high priced DVL(Doppler Velocity Log), IMU(Inertial Measurement Unit), and high accuracy range sensors. However, it is noticeable that the proposed method can achieve the accuracy which is affordable for correction of accumulated dead reckoning error, even though it uses only range data of low reliability and accuracy.