• Korean Journal of Medicinal Crop Science
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• v.18 no.6
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• pp.405-408
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• 2010

Some fatty acids(palmitic acid, stearic acid, oleic acid, linoleic acid and linolenic acid) in foxtail millet seeds were evaluated. In this study, 32 varieties of accession collected from different place of South Korea were used in order to evaluate their fatty acid composition and concentration. These samples were analyzed by GC with HP20 column. Significant variability in individual fatty acids was observed. The palmitic acid ranged from 10.9 to 13.5%, stearic acid from 2.5 to 8.3%, oleic acid from 10.0 to 15.8%, linoleic acid from 62.5 to 67.9% and linolenic acid ranged from 3.0 to 5.2%. This variability can be exploited for selection of plant and for studying the genetic variability in millet. These selections can also be utilized for genetic improvement of the crops.

• Preventive Nutrition and Food Science
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• v.9 no.4
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• pp.324-329
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• 2004

In order to investigate the molecular mechanism of $\gamma$-aminobutyric acid (GABA) production in lactic acid bacteria, we cloned a glutamate decarboxylase (GAD) gene from Lactobacillus plantarum using polymerase chain reaction (PCR). One PCR product DNA was obtained and inserted into a TA cloning vector with a T7 promoter. The recombinant plasmid was used to transform E. coli. The insertion of the product was con­firmed by EcoRI digestion of the plasmid purified from the transformed E. coli. Nucleotide sequence analysis showed that the insert is a full-length Lactobacillus plantarum GAD and that the sequence is $100\%$ and $72\%$ identical to the regions of Lactobacillus plantarum GAD and Lactococcus lactis GAD sequences deposited in GenBank, accession nos: NP786643 and NP267446, respectively. The amino acid sequence deduced from the cloned Lactobacillus plantarum GAD gene showed $100\%$ and $68\%$ identities to the GAD sequences deduced from the genes of the NP786643 and NP267446, respectively. To express the GAD protein in E. coli, an expression vector with the GAD gene (pkk/GAD) was constructed and used to transform the UT481 E. coli strain and the expression was confirmed by analyzing the enzyme activity. The Lactobacillus plantarum GAD gene obtained may facilitate the study of the molecular mechanisms regulating GABA metabolism in lactic acid bacteria.

• Korean Journal of Veterinary Research
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• v.44 no.4
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• pp.571-579
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• 2004

In order to obtain the genetic informations of the Korean isolates of porcine circovirus 2 (PCV2), nucleotide sequences of total genome of three isolates and open reading frame 2 (ORF2) of four isolates were determined and compared with those of other reference PCV2 isolates. Nucleotide sequences of 3 isolates showed over 99% homology with those of reference strain (GenBank accession no. AF027217). Point mutations were mainly determined on ORF2 regions but little on ORF1 regions. The patterns of pointmutated sites and nucleotide substitution on ORF2 regions were generally consistent between Korean isolates, and these mutated sites observed in Korean isolates were also relatively similar to those of foreign isolates. Phylogenetic analysis of nucleotide or amino acid sequences showed that there were minor branches consisting of three clusters; cluster of Korea, Canada and America, cluster of Spain and Taiwan, and the last cluster of French and China isolates. These results suggested that Korean PCV2s were probably originated from North America such as Canada or USA. The genetic informations obtained from this study could be useful for the research of diagnosis and pathogenecity of PCV2.

• Journal of the Korean Society for Library and Information Science
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• v.31 no.2
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• pp.79-93
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• 1997

The Book number, which used to distinguish an individual book from every other book in the same class for easy arrangement and retrieval, can be classified into accession order book number, alphabetical book number. and chronological book number. Nowdays' incredible developing speed of knowledge change the standard of information value, the newest is the best, and user behavior, to seek the newest first. To deal with these changes, libraries need to classify the newest information into the same place. Hoping this system can be adopted in the korean libraries, I introduce the characteristics and structures of $\ulcorner$New Chronological Book Number$\lrcorner$ which was designed to deal with library's need and examine the features of alphabetical arrangement of it.

• The Korean Journal of Archival Studies
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• no.10
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• pp.112-144
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• 2004

Oral history has value that steps over the class consciousness and party feeling that records have, and enables a general representation of a human experience. Beside, It must be managed urgently because a collection of oral history has unique and limited. However, domestic related institution do not keep management for release and use of oral history in their mind are only concentrating on accession. Lately, National History Compilation Committee passes through faithful planning procedures and is collecting an oral history collection project. A merit of this project is to have attempted an image photographing. This study is propose that to examines over the all steps to say oral history collection activity to information service and a preservation from collection planning of organization. However, this study is insufficient in the management method how it is connected if does in order to manage the oral history which is collected with a part of a record in a archival institution because this study was only for the purpose of management of oral history. This problem will be studied through a process of checking the real thing of oral history. This study proposed general methodology for oral history management, if institution had a plan of collection and management or had some collection result.

• Korea Trade Review
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• v.45 no.1
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• pp.137-153
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• 2020

On February 14, 2019, the government of Korea formally decided to consider the feasibility of joining the Comprehensive and Progressive Trans-Pacific Partnership (CPTPP) and has since been conducting bilateral consultations with individual member countries. In terms of the impact estimation, the CPTPP is actually a Korea-Japan FTA, and the most sensitive issue in the FTA is the opening of the auto industry market to Japan. Despite these circumstances, previous studies have predicted that the auto industry will be a beneficiary industry when joining the CPTPP. However, the Korean auto industry is opposed to joining the CPTPP. In order to investigate the cause of this discrepancy, this paper examines the problems of previous studies in estimating the impact of joining the CPTPP and found that the preceding study did not consider the industrial characteristics of the auto sector, especially in the context of Japan-Korea trade, and was heavily dependent on the Armington elasticity (structure) in the demand function of the GTAP CGE model. As a result, the domestic auto sector could lower prices and increase exports when joining the CPTPP. This paper attempts to precisely re-estimate the impact of joining the CPTPP on the auto sector in a way that corrects these problems by changing the CGE model and reflecting the major characteristics of the industry, with policy implications for the negotiation of CPTPP accession.

• Korean Journal of Microbiology
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• v.32 no.3
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• pp.177-181
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• 1994

The sequences of the cytoplasmic 5S rRNAs(EMBL accession number X73589 and X73890) from two polupores, Ganoderma applanatum and Schizopora paradoxa, were determined by the direct chemical method for sequencing RNA and compared to the sequences of 9 reported mushrooms. 5S rRNAs of Ganoderma applanatum and Schizopora paradoxa consisted of 118 bases and fit the secondary structure model of the 5S rRNAs of basidiomycetes proposed by Huysmans et al. Based on Kimura’s K_nuc values, the closest fungus to Ganoderma applanatum was Ceratobasidium cornigerum and the one to Schizopora paradoxa was Bjerkandera adusta. When the secondary structures of 5S rRNAs of 11 mushrooms were compared the base substitution occurred at helix regions more than at loop regions. When a phylogenetic tree was constructed using the Neighbor program of the PHYLIP package, it partially discriminated and separated the mushrooms of the Hymenomycetes by the order.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.2 no.1
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• pp.102-131
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• 1974

The author's thesis is that the types used for the large-sized characters seen in the two metal type-printed books "Kyosik chubopop karyong"(交食推步法假令) and "Yok-hak kemong yohae"(易學啓蒙要解) both printed in 1458 belong to a new metal font hitherto unnamed. The former book was compiled by Yi Sun-ji(李純之) and Kim Sok-je(金石梯) in January of 1458 in accordance to King Sejo's order. A new font was created to be used for the large-sized characters of the book. Several. months after completion of the compilation, the book was printed with mixed use of the new font and the Kabin-ja(甲寅字) for medium- and small-sized characters. The latter book had been written by King Sejo before his accession to the throne. Ascending the throne the king had his scholar-subjects examine the writing to correct it where necessary. The examination was completed in July of 1458 and printing was immediately done with the two fonts the above-mentioned, new font for the large-sized letters and the Kabin-ja for the medium- and small-sized ones. The books were granted to the scholar-subjects and the students of the Sung Kyun Kwan Academy as a royal gift. The matrix seems to have been modeled after the calligraphy of King Sejo. Because the new font was created to print the large-sized letters of the two books in 1458, it may be proper to name it "Muin-ja" using the "kanji"(干支) of the year. The author is happy to identify and include another font in the list of Korean movable types as a result of the present study.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.208-216
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• 2010

Fusarium verticillioides is an important pathogen of maize, being responsible for ear rots, stalk rots, and seedling blight worldwide. During the past decade, F. verticillioides has caused several severe epidemics of maize seedling blight in many areas of China, which lead to significant losses. In order to understand the molecular mechanisms regulating fungal development and pathogenicity in this pathogen, we isolated and characterized the gene fpk1 (GenBank Accession No. EF405959) encoding a homolog of the cAMP-dependent protein kinase catalytic subunit, which included a 1,854-bp DNA sequence from ATG to TAA, with a 1,680-bp coding region, and three introns (lengths: 66 bp, 54 bp, and 54 bp), and the predicated protein precursor had 559 aa. The mutant ${\Delta}fpk1$, which was disrupted of the fpkl gene, showed reduced vegetative growth, fewer and shorter aerial mycelia, strongly impaired conidiation, and reduced spore germination rate. After germinating, the fresh hypha was stubby and lacking of branch. When inoculated in susceptible maize varieties, the infection of the mutant ${\Delta}fpk1$ was delayed and the infection efficiency was reduced compared with that of the wild-type strain. AU this indicated that gene fpk1 participated in hyphal growth, conidiophore production, spore germination, and virulence in F. verticillioides.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.5
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• pp.606-612
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• 2012

Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore the significance of IGFBP-5 in cells of the Inner Mongolian Cashmere goat (Capra hircus), IGFBP-5 gene complementary DNA (cDNA) was amplified by reverse transcription polymerase chain reaction (RT-PCR) from the animal's fetal fibroblasts and tissue-specific expression analysis was performed by semi-quantitative RT-PCR. The gene is 816 base pairs (bp) in length and includes the complete open reading frame, encoding 271 amino acids (GenBank accession number JF720883). The full cDNA nucleotide sequence has a 99% identity with sheep, 98% with cattle and 95% with human. The amino acids sequence shares identity with 99%, 99% and 99%, respectively. The bioinformatics analysis showed that IGFBP-5 has an insulin growth factor-binding protein homologues (IB) domain and a thyroglobulin type-1 (TY) domain, four protein kinase C phosphorylation sites, five casein kinase II phosphorylation sites, three prenyl group binding sites (CaaX box). The IGFBP-5 gene was expressed in all the tested tissues including testis, brain, liver, lung, mammary gland, spleen, and kidney, suggesting that IGFBP-5 plays an important role in goat cells.