• Title/Summary/Keyword: acanthoside D

Search Result 22, Processing Time 0.031 seconds

Extraction and Purification of Acanthoside-D from Acanthopanax chilsanensis (지리산 오갈피나무로부터 Acanthoside-D의 추출 및 정제)

  • 이광진;강지훈;노경호
    • KSBB Journal
    • /
    • v.16 no.1
    • /
    • pp.71-75
    • /
    • 2001
  • Acanthoside-D, contained in Acanthopanax chilsanensis is known as a ginseng-like substance. This work was focused to set up analytical and preparative conditions for Acanthoside-D purification. The ethanol extract from the powder of the trunk of Acanthopanax chilsanensis was partitioned with hexane. A $\mu$-Bondapak $C_{18}$ (3.9$\times$300 mm, $10\mu\textrm{m}$) column was used to separate Acanthoside-D from the trunk of Acanthopanax chilsanensis. From the experimental results, the mobile phase used for isolating Acanthoside-D from the extract was water/acetonitrile/methanol=80/14/6 %(v/v). The flow rate of mobile phase was 1.0 mL/min, and UV wavelength was fixed at 210 nm. Finally on a semi-preparative column (3.9$\times$ 300 mm, $15\mu\textrm{m}$, Lichrospher 100RP-18) with the same mobile phase composition, the allowable maximum injection volume increased to 250 $\mu\ell$

  • PDF

Extraction of Acanthoside-D from Acanthopanax Cortex using Supercritical Carbon Dioxide (초임계 이산화탄소를 이용한 가시오갈피로부터 Acanthoside-D의 추출)

  • 양시중;신재순;강춘형
    • KSBB Journal
    • /
    • v.19 no.4
    • /
    • pp.284-287
    • /
    • 2004
  • The purpose of this study was to find an optimum extraction condition of acanthoside-D from acanthopanax cortex with supercritical carbon dioxide as a solvent. In this effort, effects of the extraction conditions including pressure, temperature and presence or absence of a cosolvent on the extraction efficiency were investigated. The ethanol, water or 50% methanol was used as a cosolvent whilst the operating pressure ranged from 200 bar to 300 bar. The acanthoside-D concentrations were determined by means of HPLC equipped with a UV detector. From the results, it was observed that increase of higher pressure led to the higher extraction efficiency. Further, water was found to be the best cosolvent among the entrainers tested.

Approach to the Total Synthesis of Acanthoside-D

  • Ngoc, Thyen-Truong;Park, Hae-Il
    • Proceedings of the PSK Conference
    • /
    • 2003.10b
    • /
    • pp.186.4-187
    • /
    • 2003
  • Acanthoside-D, one of major components of Acanthopanacis Cortex, is known as a ginseng-like substance. it has been known to possess diverse biological effects. Acanthoside-D has a furofuran lignan structure and the synthesis of which poses interesting and often unsolved proplems of stereocontrol. Although a few interesting syntheses providing this natural product have been reported, an intermolecular McMurry coupling - intramolecular Mitsunobu cyclization route has not yet been explored. We report here a short and efficient synthetic pathway to the total synthesis of Acanthoside-D from aryl aldehydes and methyl acrylates via Baylis-Hillman reaction, intermolecular McMurry coupling and intramolecular Mitsunobu cyclization as key reaction.

  • PDF

Effect of Different Fertilizer Ratio and Planting Dates on Growth and Acanthoside D content of Acanthopanax divaricatus and Acanthopanax koreanum

  • Lee, Jung Jong;Lee, Sang Hyun;Park, Jae Sang;Park, Chung Berm;Ahn, Young Sub;Lee, Sang Chul
    • Current Research on Agriculture and Life Sciences
    • /
    • v.31 no.2
    • /
    • pp.88-93
    • /
    • 2013
  • The cultivation methods of Acanthopanax in Korea need to be optimized. Hence, this study investigated the effect of different fertilizer ratios and planting dates on the growth and acanthoside D content of two (2) Acanthopanax species. The current recommended fertilizer rate of 10.5-8.5-8.5 $kg/ha^-$ ($N-P_2O_5-K_2O$, respectively) produced the best plant growth of Acanthopanax. For the first year, the acanthoside D content resulting from the 2P (2x phosphate) rate was higher than that from the other fertilizer ratios, yet there were no significant differences resulting from the various treatments for either Acanthopanax divaricatus or Acanthopanax koreanum. Similarly, for the second year, there were no significant differences in the acanthoside D content resulting from the various fertilizer ratios, although for both species the acanthoside D content resulting from the 2P rate was slightly higher than that from the other treatments. Therefore, the results indicated that doubling the amount of phosphate increased the acanthoside D content. Plus, the optimum planting date with respect to growth and productivity for Acanthopanax divaricatus was identified as April 15.

  • PDF

Effect of Shading and Pinching on Growth and Acanthoside-D content of Acanthopanax divaricatus var. albeofructus and Acanthopanax koreanum Nakai (차광과 적심높이에 따른 흰털오갈피나무와 섬오갈피나무의 생육과 Acanthoside-D 함량 변화)

  • Lee, Jung Jong;Lee, Sang Hyun;Park, Jae Sang;Ahn, Young Sup;Lee, Sang Chul
    • Korean Journal of Medicinal Crop Science
    • /
    • v.21 no.1
    • /
    • pp.39-44
    • /
    • 2013
  • This study was conducted to identify the effect of shading and pinching on growth and acanthoside-D content of Acanthopanax divaricatus var. albeofructus and A. koreanum Nakai. Different pinching heights showed no significant differences in terms of plant growth and acanthoside-D content but higher values showed that pinching A. divaricatus at 60 cm and A. koreanum at 30 cm favored good growth and higher fresh weight in the shoots. The content of acanthoside-D was not significantly affected by pinching heights. Also, no significant difference in acanthoside-D content was found between the lower and upper part of plant in the first year. However it was much higher in the lower part than the upper part in the second year, which indicated that the content of acanthoside-D was comparatively high in the lower part where lignification is much advanced. Shading showed benefits in terms of growth of A. divaricatus while only 50%-shading was favorable for A. koreanum to achieve superior growth. Overall, results indicated that shading had favorably affected the growth of the 2 Acanthopanax species while no-shading is better if we opt to achieve higher acathoside D content.

Isolation and Quantitative Analysis of Acanthoside D from Acanthopanacis Cortex (오가피로부터 Acanthoside D의 분리 및 함량분석)

  • Hong, Sung-Su;Hwang, Ji-Sang;Lee, Seon-A;Hwang, Bang-Yeon;Ha, Kwan-Won;Ze, Keum-Ryon;Seung, Rack-Seun;Ro, Jai-Seup;Lee, Kyong-Soon
    • Korean Journal of Pharmacognosy
    • /
    • v.32 no.4 s.127
    • /
    • pp.316-321
    • /
    • 2001
  • The Acanthopanax genus belonging to the Araliaceae family is widely used as a traditional medicine having tonic and sedative effects. For the quality control of Acanthopanacis Cortex, lignan compound, acanthoside D, was isolated from the MeOH extract of Acanthopanax sessiliforum Seeman. (Araliaceae) and identified by the spectroscopic analysis. A quantitative analysis of acanthoside D using HPLC method showed that the average contents were $0.081{\pm}0.058%$ in 39 samples collected throughout the regions of Korea.

  • PDF

Quantitative Analysis of Acanthopanacis Cortex and Achyranthis Radix for Standardization of GCSB-5 Preparation (생약복합제 GCSB-5의 품질 표준화를 위한 오가피 및 우슬의 함량 분석)

  • Lee, Eun-Hee;Cha, Bae-Cheon
    • Korean Journal of Pharmacognosy
    • /
    • v.39 no.4
    • /
    • pp.316-323
    • /
    • 2008
  • In this study, we have investigated the HPLC analysis methods and quantitative analysis of standard compounds for quality standardization of a medicinal crude drug GCSB-5, a herbal formulation consisting of 6 medicinal plants (Acanthopanacis Cortex, Achyranthis Radix, Ledebouriellae Radix, Cibotii Rhizoma, Glycine Semen, Eucommiae Cortex) which are used in traditional medicine to treat various bone disorders. HPLC analysis methods of acanthoside D(Acanthopanacis Cortex), 20-hydroxyecdysone(Achyranthis Radix) which were known standard compounds among 6 medicinal plants were developed on crude material and product. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline. Content of acanthoside D and 20-hydroxyecdysone on raw material of GCSB-5 were decided at 0.577-0.578 mg/g and 0.311-0.312 mg/g. And we confirmed that content of acanthoside D and 20-hydroxyecdysone on GCSB-5 preparation were 0.302-0.303 mg/capsule and 0.113-0.115 mg/capsule.