• Title/Summary/Keyword: acacetin

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국내산 홍화씨 부위별추출물의 이화학적특성, serotonin화합물 및 acacetin의 함량 비교

  • 김준한;김종국;강우원;김귀영;박필숙;박모라;문광덕
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.10a
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    • pp.154-154
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    • 2003
  • 국내산 홍화씨를 부위별(Whole, Coat and Endosperm)로 분리한 후 추출용매, 볶음조건, 효소가수분해 등의 처리조건을 달리하여 추출된 추출물의 이화학적특성과 기능성성분 함량을 분석, 비교하였다. 홍화씨 부위별추출물의 고형분함량은 60%에탄올배유부분추출물이 11.29%로, 19$0^{\circ}C$, 30분 볶음처리배유추출물이 14.53%로, amyloglucosidase 처리배유추출물이 24.21%로 높은 고형분이 추출되었다. 총페놀함량은 추출용매의 에탄올농도가 증가함에 따라 증가하는 경향이었고, 80% 에탄올점질추출물이 965mg%로 가장 높았으며, 21$0^{\circ}C$, 30분 볶음처리껍질추출물이 756 mg%로, celluase처리껍질추출물이 1170mg%로 높은 함량이었다. 총플라보노이드함량은 80%에탄올전체추출물이 317 mg%로, 21$0^{\circ}C$, 30분 볶음 처리배유추출물이 488 mg%로, $\beta$-amylase 처리전체추출물이 554 mg%로 높은 함량이었다. 유리당 중 sucrose함량은 21$0^{\circ}C$, 10분 볶음처리배유추출물이 123.4 mg%로, 60%에탄올배유추출물이 57.0 mg%로, Celluase 처리배유추출물이 67.1 mg%로 가장 높은 함량이었고, 또한 glucose, fructose, xylose 및 arabinose 등도 함유하고 있었다. 유기산 중 citric acid 함량은 20%n 에탄올배유추출물이 243.3 67.1 mg%로, 21$0^{\circ}C$, 30분 볶음처리전체추출물이 76.3 mg%로, amyloglucosidase 처리배유추출물이 699.3 mg%로 가장 높은 함량이었고, 또한, oxalic, malic, succinic, acetic 및 fumaric acid 등도 확인되었다. Serotonin 화합물 중 serotonin- I 함량은 100% 에탄올껍질추출물이 431 mg%로, 21$0^{\circ}C$, 10분 볶음처리껍질추출물이 192 mg%로, amyloglucosidase 처리껍질추출물이 256 mg%로 가장 많았다. 또한, Serotonin-II함량은 100%에탄올껍질추출물이 763 mg%로, 17$0^{\circ}C$, 10분 볶음처리전체추출물이 312 mg%로, amyloglucosidase 처리껍질추출물이 456 mg%로 가장 많았다. Acacetin 함량은 80%에탄올배유추출물이 34.9 mg%로, 21$0^{\circ}C$, 30분 볶음처리배유추출물이 221.0 mg%로, amyloglucosidase 처리배유추출물이 27.8 mg%로 가장 많았다.

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Serotonins of safflower seeds play a key role in anti-inflammatory effect in lipopolysaccharide-stimulated RAW 264.7 macrophages

  • Kim, Dong-Hee;Moon, Yong-Sun;Park, Tae-Soon;Son, Jun-Ho
    • Journal of Plant Biotechnology
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    • v.42 no.4
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    • pp.364-369
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    • 2015
  • Safflower (Carthamus tinctorius) seeds are wellknown traditional oriental medicines that have long been used for the remedies of blood stasis and bone formation in east Asia. In this study, ethyl acetate (EtOAc) was used for extraction of the main chemical compounds from C. tinctorius seeds. Four major compounds were identified, acacetin, cosmosiin, N-feruloyl serotonin and N-(p-coumaroyl) serotonin. Each compound was evaluated for its inhibitory activity against the inflammatory process of macrophages. All compounds significantly inhibited production of lipopolysaccharide (LPS)-stimulated nitric oxide (NO) and pro-inflammatory cytokines. The protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were dramatically decreased by serotonins in a dose-dependent manner in LPS-stimulated RAW 264.7 macrophages. These results suggest that serotonin derivatives from safflower seeds may reduce inflammation-related diseases.

Antimycobacterial Activity and Cytotoxicity of Flavonoids from the Flowers of Chromolaena odorata

  • Suksamrarn, Apichart;Chotipong, Apinya;Suavansri, Tananit;Boongird, Somnuk;Timsuksai, Puntip;Vimuttipong, Saovaluk;Chuaynugul, Aporn
    • Archives of Pharmacal Research
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    • v.27 no.5
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    • pp.507-511
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    • 2004
  • From the flowers of Chromolaena odorata (Eupatorium odoratum) four flavanones, isosakuranetin (5,7-dihydroxy-4'-methoxyflavanone) (1), persicogenin (5,3'-dihydroxy-7,4'-dimethoxyflavanone) (2), 5,6,7,4'-tetramethoxyflavanone (3) and 4'-hydroxy-5,6,7-trimethoxyfla-vanone (4), two chalcones, 2'-hydroxy-4,4',5',6'-tetramethoxychalcone (5) and 4,2'-dihydroxy-4',5',6'-trimethoxychalcone (6), and two flavones, acacetin (5,7-dihydroxy-4'-methoxyflavone) (7) and luteolin (5,7,3',4'-tetrahydroxyflavone) (8) were isolated and identified. Compound 1 exhibited moderate antimycobacterial activity against Mycobacterium tuberculosis with the MIC value of 174.8 $\mu$M, whereas compounds 4,7, and 8 exhibited weak activity with the MIC values of 606.0, 704.2 and 699.3 $\mu$M respectively. Compound 7 showed moderate cytotoxicity against human small cell lung cancer (NCI-H187) cells with the MIC value of 24.6 $\mu$M, whereas compound 8 exhibited moderate toxicity against NCI-H187 cells and week toxicity against human breast cancer (BC) cells with the MIC values of 19.2 and 38.4 $\mu$M respectively.

Phenolic Constituents and Their Anti-inflammatory Activity from Echinochloa utilis Grains

  • Nguyen, Duc Hung;Zhao, Bing Tian;Le, Duc Dat;Kim, Ki Yun;Kim, Young Ho;Yoon, Young Ho;Ko, Jee Youn;Woo, Koan Sik;Woo, Mi Hee
    • Natural Product Sciences
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    • v.22 no.2
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    • pp.140-145
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    • 2016
  • Seven phenolic compounds including p-coumaric acid (1), 4-hydroxybenzoic acid (2), 4-hydroxybenzaldehyde (3), vanillic acid (4), luteolin (5), acacetin (6), and tricin (7), were isolated from the methylene chloride and ethyl acetate fractions of Echinochloa utilis grains. Compounds (1 - 4, 6) were isolated for the first time from this plant. These compounds were tested for inhibitory activities against LPS-induced NO production in RAW 264.7 cells. Compounds 5 and 6 displayed significant inhibitory effects, with $IC_{50}$ values of $27.9{\pm}2.6$ and $14.0{\pm}1.1{\mu}M$, respectively. The results suggested that E. utilis ethanolic extract may be used as a potential source of anti-inflammatory agents and functional foods for the treatment of allergic diseases.

A review on Phytochemistry and pharmacological Activities of Cirsium setidens (Dunn) Nakai (고려엉겅퀴(Cirsium setidens (Dunn) Nakai)의 구성성분 및생리활성에 관한 리뷰)

  • MiAe Cho;Bumjung Kim
    • The Korea Journal of Herbology
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    • v.38 no.4
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    • pp.31-43
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    • 2023
  • Objectives : The objective of this study was to investigate the phytochemistry and pharmacological activities of Cirsium setidens. Methods : Domestic and international articles about Cirsium setidens were investigated. A review was perfoemed via DB searching engine such as Sci.Direct, Springer, DBpia, KISS, Google scholar, Kipris, and so on. Total 73 listed literature were classified by compound analysis and pharmacological efficacy. Results : C. setidens contains pectolinarin and its glycoside, pectolinarigenin as index compounds, and linarin, apigenin, diosmetin, scopoletin, acacetin, cirsimarin, cirsimaritin, setidenosides A and B, silymarin, hispidulin, 92 volatile compounds, and 15 fatty acids. The Pharmacological activities of C. setidens has been reported to inhibit of platelet aggregation and fat accumulation in the liver, inhibit to hepatitis, anti-cancer, antibacterial, skin improvement, hair growth, liver protection, anti-diabetic, anti-inflammatory, sedative. Also, It has been reported the effect of cholesterol-lowering and anti-obesity, neuroprotective effects, increasing human stem cell viability, inhibiting osteoclast formation and osteogenic differentiation. Conclusion : This reviews showed that C. setidens which has been traditionally used for the treatment of inflammation and hypertension, has anticancer and river protective effect, as well as hair loss and diet. In order to maximize the efficacy of C. setidens, research has also begun on the effect of processing processes such as fermentation or fine powdering and combining natural plant resources.

Changes in Flavonoid Contents of Safflower Leaf during Growth and Processing

  • Lee, Jun-Young;Park, Kyeng-Seok;Choi, Sang-Won
    • Preventive Nutrition and Food Science
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    • v.10 no.1
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    • pp.1-5
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    • 2005
  • Eight flavonoids, apigenin-6-C-β-D-glucopyranosy l-8-C-β-D-glucopyranoside (AGG), quercetin 7-O-β-D­glucopyranoside (QG), luteolin 7-O-β-D-glucopyranoside (LG), quercetin 7-O-(6'-O-acetyl)-β-D-glucopyranoside (QAG), luteolin 7-O-(6'-O-acetyl)-β-D-glucopyranoside(LAG), quercetin (Q), luteolin (L) and acacetin 7-O-β­D-glucuronide (AG) were determined by HPLC in the safflower (Carthamus tinctorius L.) leaf during growth and processing. During growth, levels of five flavonoid glycosides (AGG, QG, LG, QAG, & LAG) in the leaf increased progressively at over time according to growth stages, reached a maximum before June 11, and then decreased sharply, while those of three flavonoid aglycones (Q, L, & AG) increased greatly at the early stage of growth, reached a peak before May 28, and then decreased rapidly. During the steaming process, contents of five flavonoid glycosides increased rapidly with increased steaming time, reached a maximum after 5 min of steaming, and then decreased, whereas those of flavonoid aglycones except for AG decreased sharply with increased steaming time. During the roasting process, contents of three flavonoid glycosides decreased rapidly with increased roasting time, whereas those of two acetylflavonoid glycosides (QAG & LAG) and three flavonoid aglycones increased progressively with increased roasting time, reached a maximum after 3 min of roasting, and then decreased. These results suggest that appropriate steamed and roasted safflower leaves are a rich source of flavonoids, and may be a good source of bioactive components as a functional leaf tea.

Phytoestrogen-Induced Phosphorylation of MAP Kinase in Osteoblasts is Mediated by Membrane Estrogen Receptor

  • Park, Youn-Hee;Park, Hwan-Ki;Lee, Hyo-Jin;Park, Sun-Mu;Choi, Sang-Won;Lee, Won-Jung
    • The Korean Journal of Physiology and Pharmacology
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    • v.6 no.3
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    • pp.165-169
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    • 2002
  • We have previously demonstrated that phytoestrogens isolated from safflower seeds significantly attenuated bone loss in ovariectomized rats, and directly stimulated proliferation and differentiation of cultured osteoblastic cells. In an attempt to elucidate underlying cellular mechanisms, in the present study we investigated effects of $17{\beta}-estradiol\;(E_2)$ and phytoestrogens such as matairesinol and acacetin, a type of lignan and flavonoid, respectively, on activation of mitogen activated protein (MAP) kinases, extracellular signal-regulated kinase 1 (ERK1) and ERK2, in cultured osteoblastic ROS 17/2.8 cells. Western blot analysis with anti-MAP kinase antibody showed that a wide range concentrations $(10^{-14}\;to\;10^{-6}\;M)\;of\;E_2$ as well as both phytoestrogens induced rapid and transient activation of ERK1/2 through phosphorylation within minutes. Maximum activation of MAP kinases by $E_2$ and phytoestrogens were observed at 10 and 15 min, respectively. $E_2-induced$ phosphorylation of ERK1/2 returned to the control level at 30 min, whereas phytoestrogen-induced phosphorylation was maintained at high level until 30 min. PD-98059, a highly selective inhibitor of MAP kinase, prevented phosphorylation of ERK1/2 in the cells treated either with $E_2$ or phytoestrogens. To examine a possible involvement of estrogen receptor in the activation process of MAP kinase, Western blot analysis was performed in the presence and absence of the estrogen receptor antagonists, ICI 182,780 and tamoxifen. These antagonists blocked MAP kinase phosphorylation induced not only by $E_2,$ but also by the phytoestrogens. To the best our knowledge, this study is the first to demonstrate that phytoestrogens such as flavonoid and lignan extracted from safflower seeds produce a rapid activation of MAP kinase, at least partially via membrane estrogen receptor of the cultured osteoblastic cells.

홍화의 부위별 화학성분과 DPPH radical 소거 활성

  • 김준한;김종국;강우원;하영선;문광덕
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.04a
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    • pp.149-149
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    • 2003
  • 홍화의 식품재로로서 이용성을 높이고자 홍화의 부위별 성분분석 및 DPPH radical 소거 활성을 조사하였다. 단백질은 어린싹 부위에 28.39%, 지방은 씨 부위에 20.47%로 많이 함유되어 있었다. Glucose는 어린싹에 1,253.6 mg%, fructose는 이린싹에 970.7 mg%, sucrose는 꽃봉오리 부위에 912.0 mg%로 많이 함유되어 있었다, Succinic acid는 꽃잎 부위에 2,795.3 mg%, malic acid는 잎과 어린싹 부위에 각각 2,054.8 mg%와 934.2 mg%로 많이 함유하고 있었다. 무기질로는 K이 잎과 어린싹 부위에 각각 2,826.8 mg%와 l1999.8 mg%, Ca 또한 잎과 어린싹 부위에 각각 2,613.6 mg%와 1160.9 mg%로 많이 함유하고 있었다. 불포화지방산인 linoleic acid는 어린싹과 씨 부위에 각각 80.01%와 78.21%로 가장 많이 함유하고 있었다. 총페놀함량은 꽃잎, 어린싹, 잎 부위에 각각 5.8%, 4.4% 및 2.5%, 총플라보노이드 함량은 꽃잎, 어린싹, 잎 부위에 각각 4.7%, 6.5% 및 2.0%로 많이 함유하고 있었다. Serotonin(5-bydrnxyoyptamine, 5-HT)화합물인 serotonin-I은 홍화씨 부위에 147.7 mg%, serotonin-II 또한 홍화씨 부위에 155.4 mg%를 함유하고 있었고, flavonoid화합물인 acacetin도 홍화씨 부위에 116.5 mg%을 함유하고 있었다. 또한, luteolin은 어린싹 부위에 388.3 mg%, luteolin 7-glucoside은 잎 부위에 692.3 mg%로 많이 함유하고 있었다. DPPH radical 소거 활성은 꽃잎과 잎의 80% 에탄을 추출물이 114.2%와 113.6%의 높은 활성을 나타내어 기존의 합성항산화제인 BHA 100 ppm 농도의 88.05%의 활성 보다 높은 항산화 활성을 가지고 있음을 확인하였다.

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Inhibitory Effects of Flavonoids from Lespedeza cuneata on Aldose Reductase

  • Quilantang, Norman;Lee, Ju Sung;Yun, Young-Sok;Limbo, Carlo;Yoo, Sang Woo;Lee, Seong;Lee, Sanghyun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.62-62
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    • 2018
  • Inhibition of aldose reductase (AR) has been shown to prevent the onset and progression of many diabetic complications wherein several AR inhibitors were isolated from plants abundant in polyphenolic compounds. Lespedeza cuneata (Fabaceae), a perennial plant indigenous in East Asian countries, is shown to be abundant in these polyphenolic substances such as flavonoids and tannins. However, there are no studies to date regarding its effects on AR. In this study, the inhibitory activity of the methanol extract and stepwise polarity fractions of Lespedeza cuneata on AR was investigated. The bioactive compounds purified from L. cuneata by repeated column chromatography were also tested for AR inhibition. Results show that the ethyl acetate and n-butanol fractions of L. cuneata exhibited potent inhibition against AR with $IC_{50}$ values of 0.57 and $0.49{\mu}g/mL$, respectively. Further analysis led to the isolation of acacetin (1), afzelin (2), astragalin (3), kaempferol (4), and scutellarein 7-O-glucoside (5). The AR inhibitory effects these five compounds were also determined in which compounds 2, 3, and 5 showed potent AR inhibitory effects with $IC_{50}$ values of 2.20, 1.91, and $12.87{\mu}M$, respectively. Quantitative analysis of astragalin (3) by HPLC-UV was also performed in the leaves and roots of L. cuneata (0.626 and 0.671 mg/g, respectively). This study reports that the flavonoids isolated from L. cuneata show promising AR inhibitory activities which can be further developed as natural therapies for treating and managing diabetic complications.

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Systemic Analysis of Antibacterial and Pharmacological Functions of Scutellariae Radix (시스템 약리학적 분석에 의한 황금의 항균효과)

  • Kim, Hyo Jin;Bak, Se Rim;Ha, Hee Jung;Kim, Youn Sook;Lee, Boo Kyun;An, Won Gun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.34 no.4
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    • pp.184-190
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    • 2020
  • This study was performed to find antibacterial substances contained in Scutellariae Radix (SR) using a systems pharmacological analysis method and to establish an effective strategy for the prevention and treatment of infectious diseases. Analysis of the main active ingredients of SR was performed using Traditional Chinese Medicine Systems Pharmacology (TCMSP) Database and Analysis Platform. 36 active compounds were screened by the parameter values of Drug-Likeness (DL), Oral Bioavailability (OB), and Caco-2 permeability (Caco-2), which were based on the drug absorption, distribution, metabolism, and excretion indicators. The UniProt database was used to obtain information on 159 genes associated with active compounds. The main active compounds with antibacterial effects were wogonin, β-sitosterol, baicalein, acacetin and oroxylin-A. Target proteins associated with the antibacterial action were chemokine ligand 2, interleukin-6, tumor necrosis factor, caspase-8,9 and mitogen-activated protein kinase 14. In the future, systems pharmacological analysis of traditional medicine will be able to make it easy to find the important mechanism of action of active substances present in natural medicines and to optimize the efficacy of medicinal effects for combinations of major ingredients to help treat certain diseases.