• Animal cells and systems
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• 제2권3호
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• pp.341-349
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• 1998

To analyze the effects of surfactants on the biosynthesis of galactolipid and the composition of fatty acids, the chloroplast envelope and thylakoid membrane were cultivated in medium treated with anionic surfactants, such as linear alkylbenzene sulfonate (0.002%, LAS), a-olefin sulfonate (O.01%, AOS), and sodium lauryl ether sulfate (0.08%, SLES), respectively. During the cultivation, the chloroplast envelope and thylakoid membrane were isolated from the cells collected at the early and middle phase of the culture and the contents of their fatty acid composition were compared with the control. When treated with surfactants, the contents of total lipid MDGD methylesters, and DGDG methylesters decreased significantly when compared with the control. It was also confirmed that more unsaturated fatty acids were involved in the biosynthesis of galactolipid. The fatty acids utilized in the biosynthesis of MGDG were in the chloroplast envelope and in the control, and linoleic acid in LAS, linolenic acid and oleic acid in AOS, and linolenic acid and oleic acid in SLES. The fatty acids in the biosynthesis of DGDG were linolenic acid and oleic acid in the control linolenic acid and stearic acid in LAS, oleic acid and linolenic acid in AOS, oleic acid and linolenic acid in SLES. In the thylakoid membrane, the major fatty acids in the biosynthesis of MGDG were linolenic acid and oleic acid in the control, oleic acid and linolenic acid in LAS, linolenic acid and linoleic acid in AOS, linolenic acid and palmitoleic acid in SLES. The fatty acids in the biosynthesis of DGDG were linolenic acid and oleic acid in the control, oleic acid and linolenic acid in LAS, linolenic acid and linoleic acid in AOS, palmitoleic acid and oleic acid in SLES.

• Journal of Nutrition and Health
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• 제26권7호
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• pp.839-850
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• 1993

This study investigated the hypolipidemic and antithrombotic effects of linolenic acid derived from Korean perilla oil. The experimental rats(male, Sprague-Dawley) were divided into 5 groups using a Randomized Complete Block Design and fed one of the five following diets : DO*/O#. D4/O, D4/4, D4/8, or D4/20(D*/# represents the ratio of linoleic to linoenic acid as the percentage of total dietary energy intake) for 4 or 8 months. Bleeding time and whole blood clotting time were determined and the composition of serum and platelet lipids analyzed. Comparisons from the DO/O to the D4/20 group showed that serum lipids (total lipid, triglyceride, total cholesterol, and HDL-cholesterol) gradually decreased with increasing linolenic acid intake - the hypolipidemic effect. The composition of platelet fatty acids[the ratio of eicosapentaenoic acid(EPA)/arachidonci aci(AA)] increased gradually with increasing linolenic acid intake. Higher linolenic acid intake increased bleeding time and whole blood clotting time, and decreased malondialdehyde(MDA) production in the platelets, though no significant differences. These results suggest that linolenic acid derived from perilla oil appears to suppress the conversion of linoleic acid to AA and the EPA transformed from linolenic acid appears to suppress the conversion of AA to TXA2. Since TXA2 is a platelet-aggregating and vasoconstricting agent, the redulction of TXA2 released by platelets with increasing intake of perilla oil containing a lot of linolenic acid confers an antithrombotic effect.

• 한국식품영양과학회지
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• 제40권12호
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• pp.1734-1742
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• 2011

본 실험에서는 들기름에 약 60%로 존재하는 ${\alpha}$-linolenic acid를 알칼리 이성질화 반응을 이용하여 CLnA(conjugated linolenic acid)와 CLA(conjugated linoleic acid)를 생산하고자 하였다. 들기름을 가수분해한 뒤에 10분에서 6시간까지의 반응시간과 5%에서 60%까지의 NaOH 농도에서 CLnA와 CLA의 생성을 표준물질과 함께 GC와 spectrophotometer를 이용하여 확인하였다. 시간에 따른 CLnA와 CLA 함량 변화는 나타나지 않았으나, NaOH 농도가 증가함에 따라서 CLnA와 CLA가 유의적으로 증가하는 경향을 보였다. 반응조건 중, 20% KOH, $180^{\circ}C$, 1시간 반응에서 14.5%와 14%의 CLnA와 CLA의 함량을 나타냈으며, 42.2%의 C18:3 conjugated 이성질체가 생성되었다. 이성질체를 확인하기 위해 GC-MS 그리고 FT-IR을 이용한 실험을 수행하였다. GC-MS의 ion peak 분석 결과, conjugated 형태에서만 특징적으로 나타나는 m/z=91 ion peak를 확인할 수 있었고, CLA와 CLnA의 분자량이 C18:2와 C18:3과 일치하는 것을 확인할 수 있었다. 그리고 NIST Library에서 검색된 methyl 9c, 11t-CLA, methyl 10t, 12c-CLA와 methyl 9cis, 11trans, 13trans-octadecatrienoate의 결과와 일치하는 ion peak를 형성하였다. 이 외의 이성질체는 FT-IR 분석 결과, 높은 trans fatty acid 함량을 보여 알칼리 이성질화 반응 시, $180^{\circ}C$의 높은 열에 반응을 하여 trans 형태의 conjugated form이 다량 생성된 것으로 사료된다. conjugated 이성질체의 생성 양을 높이기 위해 들기름 가수분해물을 우레아 분별을 한 뒤에, ${\alpha}$-linolenic acid의 함량을 70%까지 높인 뒤에 알칼리 이성질화 반응을 수행한 결과 CLnA의 생성 양이 16.6%까지 증가한 것을 확인할 수 있었다. 그러나 CLnA 이외의 conjugated 지방산 함량의 변화는 없었다.

• 한국응용과학기술학회지
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• 제24권2호
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• pp.196-204
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• 2007

The objective of this study was to determine the effect of dietary oils on the levels of the ${\gamma}-linolenic$ acid in chicken meat lipids. Three hundred ten five, 1-d old, male, Ross strain, broiler chicks were fed for 35 d to compare diets containing evening primrose oil(EPO) and hemp seed oil(HO) to a control diet. Fatty acid composition of lipid from chicken skin, thigh and breast muscle were determined at the end of the trial. The level of ${\gamma}-linolenic$ acid of lipids from chicken meat fed diets containing EPO or HO was significantly higher than that of the control group(p<0.05). The level of ${\gamma}-linolenic$ acid of lipids from chicken skin was highest in the group, which had been fed the EPO 0.85%, followed in order by EPO 0.7%, 0.5%, EPO mixed oil, HO and HO mixed oil. There was a significant difference in the level of ${\gamma}-linolenic$ acid of chicken skin between the control and treatment groups(p<0.05). The level of ${\gamma}-linolenic$ acid of lipids from chicken thigh muscle was also similar to skin, and significantly higher than that of the control group(p<0.05). The level of ${\gamma}-linolenic$ acid of lipids from chicken breast muscle was highest in the group, which had been fed the EPO 0.5%, followed in order by EPO 0.7%, 0.85%, HO 0.5% and HO mixed oil. There was a significant difference in the level of ${\gamma}-linolenic$ acid of chicken breast muscle between the control and treatment groups(p<0.05).

• Journal of Nutrition and Health
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• 제23권7호
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• pp.477-491
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• 1990

To observe the effect of dietary n6 linoleic acid, n6 gamma-linolenic acid and n3 alphalinolenic acid aon plasma lipid composition and platelet aggregation, twenty college women were divided into 4 groups and treated for 2 weeks with experimental diets supplying fat at 23% cal which were different only in fatty acid composition. Dietary fat was corn oil(CO) as a source of n6 linoleic acid(LA), perilla oil(PO) for n3 alpha-linolenic acid(ALA) and evenign primrose oil(EPO) for n6 gamma-linolenic acid(GLA). Plasma cholesterol level was slightly decreased by PL(13.5g) but significantly increased by equal amount of CO. However, there was similar hypocholeaterolemic effect when double amount of CO(27.0g), was supplemented. Therefore, total fat unsaturation may be more important factor for plasma cholesterol-lowering effect than the structure of fatty acid itself. Plasma cholesterol level was not lowered by supplement of GLA in CO diet. There was similar trend in hypotriglyceridemic effect by PO and CO as in plasma cholesterol. Plasma TG level was rather increased but not significantly by GLA supplement to CO diet. Overall, plasma lipid-lowering effect was greater by ALA than LA and GLA effect was not greater than by LA. GLA supplement did not significantly improve lipid compositions to prevent against CHD. There was no significant change both in fatty acid composition in platelet and ADP-induced platelet aggregation by GLA supplement to corn oil diet and by ALA in PO diet in young women.

• 한국식품과학회지
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• 제21권6호
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• pp.808-814
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• 1989

Mortierella isabellina IFO 8183을 이용하여 ${\gamma}-linolenic$ acid를 생산하기 위한 최적 배양조건을 검토한 결과, 최적 탄소원과 최적 질소원은 각각 glucose와 $NH_4NO_3$이었으며, 지질 생산배지내의 최적 C/N율은 40이었다. 또한, 최적 초기 pH와 배양온도는 각각 6.0과 $30^{\circ}C$이었으며, 균체내에 축적된 지질의 지방산 조성은 pH와 온도에 의해 변화되었다. Sodium acetate를 배양 초기에 0.5%(w/v) 첨가하였을 때 대조구에 비해 ${\gamma}-linolenic$ acid 함량과 ${\gamma}-linolenic$ acid 생성량이 각각 15.4%와 37.8% 증가되었다. 최적 조건하에서 8일간 진탕배양하였을 때 균체수율, 지질수율, 지질함량, ${\gamma}-linolenic$ 함량 및 ${\gamma}-linolenic$생성량은 각각 0.347g/g, 0.18g/g, 0.52g/g, 60mg/g 및 347mg/l이었다.

• 한국축산식품학회지
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• 제23권2호
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• pp.145-149
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• 2003

마유의 지방은 적은 양의 stearic acid과 palmitoleic acid를 가지고 있으며 반면에 많은 양의 linolenic acid과 linolic acid를 가지고 있다. 마유와 우유의 지방산을 비교하였을 때, oleic acid (C18:1)의 양은 차이가 없었으며, 불포화 지방산 성분인 linoleic acid와 linolenic acid은 마유가 우유에 비해 상대적으로 높았으며 마유 크림에서의 linoleic acid (C18:2) 는 우유보다 약 7배, linolenic acid (C18:3)은 약 50배 높은 것을 관찰하였다. 마유와 우유의 시료를 기간별로 실험한 결과 마유의 시료를 채취한 후 45일 동안 linoleic acid와 linolenic acid를 우유와 비교하였을 때 매우 높게 유지되었으며 이 결과 음식물로만 섭취할 수 있는 이 두 성분이 에너지원, 산소공급원으로 매우 유용하게 작용한다는 것을 알 수 있었다.

• 한국동물생명공학회지
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• 제37권1호
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• pp.17-26
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• 2022

Alpha-linolenic acid is an important polyunsaturated fatty acid that exhibits anticancer, anti-inflammatory, and antioxidative effects. In this study, we investigated the protective effects of alpha-linolenic acid on the cell proliferation and differentiation of C2C12 cells under essential amino acid-deficient conditions. Different concentrations of alpha-linolenic acid and essential amino acids were added to the growth and differentiation media. The concentrations of 10 µM of alpha-linolenic acid and 2% essential amino acid were chosen for subsequent experiments. Supplementation with alpha-linolenic acid and essential amino acids improved the proliferation and differentiation of C2C12 cells and significantly increased the mRNA levels of catalase, superoxide dismutase, B-cell lymphoma-2, and beclin-1 as well as the protein levels of PPARγ coactivator-1α compared to those in the controls. Moreover, supplementation with alpha-linolenic acid and essential amino acids reduced the levels of phosphorylated H2A.X variant histone, Bcl-2-associated X, p53, and light chain 3 during C2C12 cell proliferation, and increased the expression levels of myogenic factors 4 (myogenin) and 5 during C2C12 cell differentiation. Overall, we determined that alpha-linolenic acid and essential amino acids maintained the cell proliferation and differentiation of C2C12 cells via their anti-oxidative, anti-apoptotic, and anti-autophagic effects.

• 한국식품과학회지
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• 제24권4호
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• pp.326-329
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• 1992

${\gamma}-linolenic$ acid를 함유한 유지를 생산하기 위해 포도당을 주기적으로 첨가하면서 Mucor sp. KCTC 8405P를 유가배양하였다. 배양 시작 후 2일까지 효모 모양으로 배양한 후 배지의 pH를 높여 균사체 모양으로 유도하여 배양하면, 지방질은 포도당이 고갈되는 배양 7일째까지 축적되었다. 본 배양조건에 의하여 건조 균체량 99.3 g/l, 총지방질 38.0 g/l와 3.5 g/l의 ${\gamma}-linolenic$ acid를 얻을 수가 있었다.

• 한국작물학회지
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• 제47권
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• pp.150-162
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• 2002

1. 들깨는 용도면에서 볼 때 종실은 식용으로, 기름은 식용과 공업용, 깻잎은 채소로 또 깻잎의 정유성분은 향료로 이용될 수 있는 다용도 작물이다. 2. 종실의 단백질 함량은 18-28% 정도이고 단백질의 아미노산조성에 있어 글루타메이트(glutamate)함량은 계란보다 월등히 많다. 3. 종실의 기름 함량은 35~54%로서 기름중에는 리놀렌산(linolenic acid)이 60% 이상 함유되어 있으므로 생리적 기능성 식품으로 우수한 특성을 갖고 있으며 필수지방산의 공급원으로서도 중요하다. 4. 기름의 특성상 들기름은 고도의 불포화 지방산인 리놀렌산이 주성분이기 때문에 들기름을 짜서 상온에서 보관하게 되면 산패(oxidation)되기 쉬우므로 장기간 저장 이용하기 어렵다. 5. 깻잎은 비타민 C와 베타카로틴($\beta$-carotene) 함량이 많고 다른 미량성분도 풍부하게 함유되어 있으므로 들깨잎은 생식용 채소로서의 이용가치가 높다. 6. 깻잎의 정유(essential oil) 성분은 페릴라알데하이드(perilla aldehyde)가 주성분이며 이는 식품이나 식품첨가제로 쓰일 때 광범위한 항균작용(antimicrobial activity)을 나타낸다. 7 들깨나 자소잎의 붉은 색소중에는 안토시아닌(anthocynins)이 다량 함유되어 있으며 이는 식품색소로 널리 쓰인다.