• Journal of the Society of Naval Architects of Korea
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• v.40 no.3
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• pp.45-53
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• 2003

Ships and ocean structures are subjected to random loads caused by irregular waves. The irregularity of amplitude from random loading affects on fatigue crack growth and fatigue life. However the effects of irregularity of loading on fatigue including random loading have not been explained exactly. Therefore in this paper crack growth tests on DENT specimens under constant-amplitude loading including a single tensile overload are conducted to investigate the effect of overload on crack growth rate. The size of plastic zone and crack growth rate before and after a single tensile overloading are measured using ESPI system. Crack growth retardation model that is characterized by crack growth length and the size of plastic zone was proposed and compared with test result. From the research, the validity of proposed model is examined on crack growth retardation, and consequently fatigue life.

• Journal of Microbiology and Biotechnology
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• v.28 no.2
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• pp.298-304
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• 2018

The single-vessel multienzyme UDP-${\alpha}$-$\text\tiny{D}$-glucose recycling system was coupled with a forward glucosylation reaction to produce novel glucose moiety-conjugated derivatives of different tetracycline antibiotic analogs. Among five tetracycline analogs used for the reaction, four molecules (chlorotetracycline, doxytetracycline, meclotetracycline, and minotetracycline) were accepted by a glycosyltransferase enzyme, YjiC, from Bacillus licheniformis to produce glucoside derivatives. However, the enzyme was unable to conjugate sugar units to rolitetracycline. All glucosides of tetracycline derivatives were characterized by ultraviolet absorbance maxima, ultra-pressure liquid chromatography coupled with photodiode array, and high-resolution quadruple time-of-flight electrospray mass spectrometry analyses. These synthesized glucosides are novel tetracycline derivatives.

• Korean Journal of Health Education and Promotion
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• v.25 no.1
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• pp.39-53
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• 2008

Objectives: The purpose of this study was to investigate the effects of muscle strength exercise on physical function and quality of life in the frail elderly. Methods: Subjects were 401 frail elderly people selected by the screening tool developed by Japan department of Health, Labor and welfare. The program was carried out for twice a week for 12 weeks. Data were analyzed with paired t-test and t-test using STATA program. Results: 1) Muscle strength exercise for the frail elderly showed statistically significant effects on improving grip strength, single leg stand, TUG(Time up to go) and quality of life. 2) The grip strength and single leg stand indicators showed statistically significant effects between the over 75 years old and the under 75 years old. However, TUG and quality of life indicators didn't show statistically significant effects between the over 75 years old and the under 75 years old. Conclusions: The muscle strength exercise for the frail elderly people was very effective on improving grip strength, single leg stand, TUG and quality of life and especially, it was more effective in the over 75 years old than in the under 75 years old.

• Journal of Korean Academy of Nursing
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• v.52 no.3
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• pp.324-340
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• 2022

Purpose: This study determined acculturative stress' effect on the life satisfaction of multicultural adolescents based on Roy's Adaptation Model and some earlier studies. Further, it examined the sequential multiple mediating effects of bicultural acceptance attitude, self-esteem, and social withdrawal on life satisfaction. Methods: Participants included 1,163 multicultural adolescents who participated in the sixth Multicultural Adolescents Panel Study. A hypothesis test was conducted using Hayes' Process Macro Model 81. Results: Life satisfaction increased with a decline in acculturative stress. Each of bicultural acceptance attitude, self-esteem, and social withdrawal had a single mediating effect on the relationship between acculturative stress and life satisfaction in multicultural adolescents. The sequential multiple mediating effects of bicultural acceptance attitude and self-esteem were confirmed significant after their impact on the relationship between acculturative stress and life satisfaction was analyzed. Bicultural acceptance attitude and social withdrawal were found to have a significant sequential multiple mediating effect on the relationship, as well. Conclusion: This study's results demonstrate that acculturative stress reduction is critical to improving multicultural adolescents' life satisfaction. Bicultural acceptance attitude, self-esteem, and social withdrawal have a single mediating or sequential multiple mediating effect on the relationship between multicultural adolescents' acculturative stress and life satisfaction. The findings, which highlight mediating effects, indicate that by increasing bicultural acceptance attitude and self-esteem, and reducing social withdrawal, multicultural adolescents' life satisfaction can be improved.

• Journal of the Korean Society of Propulsion Engineers
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• v.26 no.6
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• pp.43-52
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• 2022

Operational reliability of the single-use turbo engine for guided weapons must be guaranteed even after long-term storage. Rolling element bearings have a great influence on the operational reliability of the turbo engine, however changes in micro dimensions of bearings by an oxide layers on rolling elements and raceways may cause failures after long-term storage. In this study, changes in dimensions of bearings were measured and roughness of rolling elements was used for estimating the storage life. Storage life estimation was performed via two kinds of methods, Weibayes method and random sample generation method. The results of two methods were compared and their characteristics were analyzed. This study will contribute to establish an efficient maintenance schedule for the single-use turbo engine.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.226-230
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• 2022

The sheep can be reproduced by natural mating as well as applied reproductive biotechnology, embryo transfer (ET). However, this method in sheep is influenced by several factors such as season, photoperiod, latitude, temperature, nutrition, and breed. In addition, there is still less research on assisted reproductive technologies in small ruminants, compared to other livestock species such as cattle and pigs. Because there has been a need for an optimization and a continuous improvement of ET techniques in small ruminants. the main objective of this study was to evaluate the conception rate obtained after ET in Mongolian sheep (Dorper breed). After embryo recover, code 1 and 2 embryos (morula or blastocyst stage) for ET in the present study were 63% (63/100) and 24% (24/100), respectively. Then Each single embryo was transferred to a synchronized recipient who prepared by estrous synchronization protocol with fluorogestone acetate-cloprostenol sodium. The results demonstrated that an average conception rate and lambing rate was 35.6% (31/87) and 33.3% (29/87), respectively. Further study is still necessary, but these results indicated that single embryo of Mongolian sheep with the present protocol was enough to conducting ET when the genetically superior sheep were necessary to be expanded.

• Journal of Ginseng Research
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• v.36 no.4
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• pp.442-448
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• 2012

Somatic embryogenesis is one of good examples of the basic research for plant embryo development as well as an important technique for plant biotechnology such as medicinally important plants. Single embryos develop into normal plantlets with shoots and roots. Therefore, direct single embryogenesis derived from single cells is highly important for normal plant regeneration. Here we demonstrate that the cyclic secondary somatic embryogenesis in Panax ginseng Meyer is a permanent source of embryogenic material that can be used for genetic manipulations. Secondary somatic embryos were originated directly from the primary somatic embryos on hormone-free Murashige and Skoog medium, and proliferated further in a cyclic manner. EM medium (one third of modified MS medium [MS medium containing half amount of NH4NO3 and KNO3] with 2% to 3% sucrose) favored further development of proliferated secondary somatic embryos into plantlets with root system. The plantlets developed into plants with well-developed taproots in half-strength Schenk and Hildebrandt basal medium supplemented with 0.5% activated charcoal.

• Korean Journal of Human Ecology
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• v.7 no.2
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• pp.11-22
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• 1998

Social support may be a crucial aspect in single mothers' adjustment from the loss of spouse. Using a survey data collected from 285 widowed or divorced mothers, this study investigated whether social support buffers the deleterious effects of stress on psychological adjustment among poor single mothers. Results revealed that social support is not provided for at a desirable level. After controlling the single mothers' characteristics, stress in daily life, emotional support, job status and child relationship were associated with depression of them. It was also founded that emotional support buffer the negative effect of stress on depression of single mothers. Therefore, the support programs focused on emotional support are necessary for single mothers to enhance their mental health.

• Proceedings of the KIEE Conference
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• 1996.07c
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• pp.1707-1709
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• 1996

For the improvement of the performance and life time of phosphoric acid fuel cell, the management of electrolyte in the electrocatalyst layer and electrolyte matrix in the cell structure is very important. Porous bipolar pinto structure, that is known as an advanced type, is generally used for the storage of electrolyte in the cell. In this paper, the single cell was made of the electrode by coating directly electrocatalyst layer on porous bipolar plate. The single cell showed $186\;mA/cm^2$ at 0.6V. This performance is similar to the performance of the conventional nonporous plate single cell. The technology of porous plate single cell could be directly used to the fabrication of stack in order to improve the performance and life time of phosphoric acid fuel cell.

• BMB Reports
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• v.54 no.10
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• pp.505-515
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• 2021

Human pluripotent stem cells (hPSCs) include human embryonic stem cells (hESCs) derived from blastocysts and human induced pluripotent stem cells (hiPSCs) generated from somatic cell reprogramming. Due to their self-renewal ability and pluripotent differentiation potential, hPSCs serve as an excellent experimental platform for human development, disease modeling, drug screening, and cell therapy. Traditionally, hPSCs were considered to form a homogenous population. However, recent advances in single cell technologies revealed a high degree of variability between individual cells within a hPSC population. Different types of heterogeneity can arise by genetic and epigenetic abnormalities associated with long-term in vitro culture and somatic cell reprogramming. These variations initially appear in a rare population of cells. However, some cancer-related variations can confer growth advantages to the affected cells and alter cellular phenotypes, which raises significant concerns in hPSC applications. In contrast, other types of heterogeneity are related to intrinsic features of hPSCs such as asynchronous cell cycle and spatial asymmetry in cell adhesion. A growing body of evidence suggests that hPSCs exploit the intrinsic heterogeneity to produce multiple lineages during differentiation. This idea offers a new concept of pluripotency with single cell heterogeneity as an integral element. Collectively, single cell heterogeneity is Janus-faced in hPSC function and application. Harmful heterogeneity has to be minimized by improving culture conditions and screening methods. However, other heterogeneity that is integral for pluripotency can be utilized to control hPSC proliferation and differentiation.