• Microbiology and Biotechnology Letters
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• v.18 no.4
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• pp.406-410
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• 1990

The influence of glucose concentration on cell growth rate and on the expression level of the strong promoter obtained from alkali-tolerant Bacillus sp. YA-14 chromosomal DNA was studied. In fed-batch culture, the promoter activity could be maximized by maintaining a very low level of glucose concentration in the broth and glucose consumption rate below 1.08g/g cell-h. The induction of the promoter was possible by addition of sporulation medium after the cell was grown in growth medium with only low level of CAT activity.

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2008.05a
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• pp.111-111
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• 2008

• Natural Product Sciences
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• v.9 no.3
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• pp.183-185
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• 2003

The combination of hexane extract (E4) of rhizome of Acorus gramineus with chloramphenicol (Cm) was applied to Gram negative Cm resistant microbials to find the possibility of clinical use and to clarify the relationship of the activity of chloramphenicol acetyltransferase (CAT). The combination of $1,000\;{\mu}g/ml$ of E4 and $8\;{\mu}g/ml$ of Cm entirely ceased the growth of S. aureus SA2, a gram positive resistant strain to 10 antibiotics. But in Gram negative strains which possess CAT activity, some showed considerably strong resistances to Cm and some did weakly.

• Proceedings of the Korean Society of Veterinary Clinics Conference
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• 2009.10a
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• pp.238-238
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• 2009

• Korean Journal of Veterinary Research
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• v.39 no.2
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• pp.370-375
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• 1999

Owner's attitudes for tendonectomy, the advantages of this surgical technique, and postoperative complication were investigated by telephone survey. 18 cats on whom tendoncetomy was performed with or without concurrent ovario-hysterectomy or castration were included in this study. The first reason for tendonectomy was to avoid damage caused by the cat's scratching household materials. The first benefit of tendonectomy was decreasing damage to materials (89%). The primary concern of the owners of cats that underwent tendonectomy was postoperative pain after surgery (61%). Twelve cats (67%) that underwent tendonectomy recovered fully within the first three days and 6 cats (33%) recovered within two weeks. After combining the very positive rating and positive as positive, seventeen owners (94%) of cats that underwent tendonectomy had a positive attitude to the surgery.

• Journal of Applied Biological Chemistry
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• v.43 no.1
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• pp.1-4
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• 2000

Chloramphenicol acetyl CoA transferase (CAT) and angiotensin- converting enzyme inhibitory peptide (ACEI) were fused to C-terminal region of rice ubiquitin to examine the level of transcripts or enzyme activities in yeast. When two chimeric genes under an inducible Gall promoter control were transformed into Saccharomyces cerevisaie, both CAT and ACE inhibitory activities were enhanced by three to four-fold as compared to those containing no ubiquitin gene. However, the levels of transcripts of ubiquitin fused and un fused genes were not significantly different each other. Therefore, it was suggested that the expression of foreign genes was post-transcriptionally enhanced by fusion of plant ubiquitin in heterologous organisms such as yeast.

• Annual Conference of KIPS
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• 2018.10a
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• pp.666-668
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• 2018

최근 반려동물을 키우는 가구 수의 증가와 함께, 반려묘에 대한 관심도 상당히 증가하고 있다. 특히 반려인은 반려묘와의 원활한 의사소통과 교감을 바라지만 반려묘의 세세한 감정 상태를 24시간 내내 파악하는 것은 어려운 일이다. 본 논문에서는 반려묘의 울음소리에 많은 감정 및 상태 정보가 담겨있는 것에 착안하여, 반려묘의 울음소리를 기반으로 감정을 분류하는 시스템을 제안한다. 제안된 시스템은 먼저, 이미 수집된 소리 데이터를 데이터 증폭 방법론을 이용하여 데이터를 확장 한 후, 해당 소리들의 멜 스펙트로그램 정보를 추출한다. 이를 시계열 정보 처리에 효과적인 LSTM에 적용하여 반려묘의 감정 상황을 식별할 수 있도록 학습을 수행한다. 실험 결과, 반려묘의 감정 상태 분류의 가능성을 확인하였다.

• Annual Conference of KIPS
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• 2022.11a
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• pp.752-754
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• 2022

본 논문에서는 고양이의 생활환경을 개선하고 건강 질환을 예방하여, 반려묘를 기를 때의 부담을 감소시킬 수 있는 '반려묘 돌봄 서비스를 제공하는 지능형 캣타워'를 제안한다. 지능형 캣타워는 첫째, 고양이 나이·품종·체중에 따라 발판 높이를 조절하여 추락사고를 방지하고 관절염을 예방한다. 둘째, 캣타워에 설치된 로드셀·암모니아·인체감지 센서를 이용하여 고양이의 체중, 소변, 식사량, 운동량을 측정한다. 셋째, 캣타워는 측정한 수치를 바탕으로 건강상태를 확인하고, 사료 종류와 급여량 등을 조절하여 비만, 신장 질환 등을 예방할 수 있다.

• Journal of Life Science
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• v.28 no.3
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• pp.351-356
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• 2018

L-Serine dehydratase (LSD) is an iron-sulfur containing enzyme that catalyzes the conversion of L-serine to pyruvate and ammonia. Among the bacterial amino acid dehydratases, it appears that only the L-serine specific enzymes utilize an iron-sulfur cluster at their catalytic site. Moreover, bacterial LSDs are classified into four types based on structural characteristics and domain arrangement. To date, only the LSD enzymes from a few bacterial strains have been studied, but more detailed investigations are required to understand the catalytic mechanism of various bacterial LSDs. In this study, LSD type II from Mycobacterium tuberculosis (MtLSD) H37Rv was expressed and purified to elucidate the biochemical and catalytic properties using the enzyme kinetic method. The L-serine saturation curve of MtLSD exhibited a typically sigmoid character, indicating an allosteric cooperativity. The values of $K_m$ and $k_{cat}$ were estimated to be $59.35{\pm}1.23mM$ and $18.12{\pm}0.20s^{-1}$, respectively. Moreover, the plot of initial velocity versus D-serine concentration at fixed L-serine concentrations showed a non-linear hyperbola decay shape and exhibited a competitive inhibition for D-serine with an apparent $K_i$ value of $30.46{\pm}5.93mM$ and with no change in the $k_{cat}$ value. These results provide insightful biochemical information regarding the catalytic properties and the substrate specificity of MtLSD.

• Korean Journal of Psychosomatic Medicine
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• v.25 no.1
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• pp.63-72
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• 2017

Objectives : Attention-deficit hyperactivity disorder(ADHD) is characterized by significant impairments in executive functions, with a prevalence of approximately 3-5% of all children worldwide. The goal of this study was to examine the relationship between executive functions and electrophysiological activities in children and adolescents with ADHD. Methods : In 31 patients with ADHD, resting-state EEG was recorded, and Comprehensive Attention Test(CAT), Stroop Color-Word Inference Test(Stroop CWIT), Trail Making Test(TMT), and Wisconsin Card Sorting Test(CST) were administered. Korean version of the ADHD Rating Scale(K-ARS) was assessed. Results : Alpha and beta power positively correlated with the Attention Quotient(AQ), while delta power negatively correlated with AQ from CAT. In the Stroop CWIT, decreased delta power and increased beta power were related to higher performance. Power of the alpha band increased with higher TMT performance. Moreover, delta power negatively correlated with good performance on the CST, while alpha and high gamma band showed a positive correlation. Correlation with the parent-rating of ADHD symptoms showed a negative correlation between alpha power and higher scores on the K-ARS. Conclusions : These findings indicate that relative power in higher frequency bands of EEG is related to the higher executive function in children and adolescents with ADHD, while the association with the relative power in lower frequency bands of EEG seem to be vice versa. Furthermore, the findings suggest that QEEG may be a useful adjunctive tool in assessing patients with ADHD.