• Journal of the Korea Organic Resources Recycling Association
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• v.31 no.4
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• pp.13-28
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• 2023

This study assessed the biochemical methane potential (B_u-P) of three grass species-Poa pratensis (PP), Zoysia japonica (ZJ), and Agrostis stolonifera (AS). B_u-P values were determined as 0.330 Nm³/kg-VS_added for PP, 0.297 Nm³/kg-VS_added for ZJ, and 0.261 Nm³/kg-VS_added for AS. Notably, PP exhibited superior suitability for methane production. The investigation also examined the impact of silage storage duration on PP grass clippings, revealing a 19% decline in B_u-P from an initial value of 0.269 Nm³/kg-VS_added on day 0 to 0.217 Nm³/kg-VS_added on day 180. Throughout the storage period, there were significant increases in neutral detergent fiber (NDF), acid detergent fiber (ADF), and crude protein (CP) contents, rising from 67.59%, 39.68%, and 3.02% on day 0 to 77.12%, 54.65%, and 6.24% on day 180, respectively. These findings highlight the influence of storage duration on the anaerobic digestibility of PP grass clippings. To effectively utilize grass clippings as a renewable resource for methane production, further studies considering factors such as initial moisture content, pretreatment methods, and potential effects of residual pesticides are necessary to optimize anaerobic digestion efficiency for herbaceous biomass.

• Journal of Plant Biotechnology
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• v.4 no.4
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• pp.135-141
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• 2002

Agrobacterium-mediated transformation has been unsuccessful for monocot plants except for a few important crops such as barley, rice, maize and wheat. We discussed here that a successful transformation of monocots demands certain critical conditions. The requirements for an efficient transformation are a selection of target tissues competent for plant regeneration and Agrobacterium-infection, and various factors promoting Agrobacterium-infection. The factors were divided into two to activate Agrobacterium and to increase plant cell's susceptibility against Agrobacterium. Optimization of these factors significantly increased transformation efficiency of zoysia grass and rice plants. A technical improvement in transformation system for monocots will promote improvement of the breed as well as a study of gene functions in monocots.

• Asian Journal of Turfgrass Science
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• v.8 no.3
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• pp.149-165
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• 1994

This report was investigated on the soil and mineral nutrient erosions in comparison among the vegetation floors of M isranthus sisensis, Arternisia stelleriana, Rhododendron mucronula turn, Zoysia ja-ponira and Pinus deusitlora communities and the naked soil on Mt. Keum-hak in Cheolwon-Koon, The erosion of clay, silt, fine sand and coarse sand of the surface soils under the Z. japonira grass-land was far less then those of M. sinensis, A. stelleriana, R. murronulatum and P. densiflora com-munities and the naked soil, The loss of mineral nutrients due to the soil erosion was the lowest level in the Z. japonira grassland and the highest level in the P. densiflora forest and the naked soil, respectively.

• Asian Journal of Turfgrass Science
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• v.9 no.3
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• pp.225-234
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• 1995

The Widespread and most abundant grasses are Miscanthus sinensis and Pteridium aquilinum in the grassland of Cheju-Island. The vascular flora of the grassland are composed of 127 species in all. Those communities of Miscanthus sinensis Pteridium aquilinum Imperata cylindrica Themeda japon-ica and Zoysia japonica are relatively homogeneous. Standing crops of the mixed grass communities range from 657 g /$m^2$ to 2, 975 g /$m^2$ on August 30, and the value of daily net production is around 2.6~121 g/$m^2$. The standing crop of north facing slop showed higher than that of south facing slop. Relationship between the standing crop and water content, organic matter, total nitrogen and available phosphorus of soils in Cheju-Island showed high level of significant.

• Korean Journal of Plant Resources
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• v.11 no.3
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• pp.315-318
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• 1998

This study was carried out to investigate the effect of seedling growth with different seedling rate for machine transplanting with turf. Chosun Zoysia was sown on May 17 with three levels seedling rates. The plant charactersitics were greater in 500g/box plots than the other seedling rates on the seedling box. It ws shown that the 30-40day old seedlings of 1.000 and 2,000g/box seedling plots appeared to be proper methods for Sprigging as the high seedling density. The 70 day old seedling plant with 500g/box were shown to be propoer methods for Sprigging. The seedling periods need to be 60 days for 500g/box plots and 40 days for 1,000 and 2,000g/box plots as forming the mats.

• Journal of Plant Biotechnology
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• v.43 no.4
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• pp.450-456
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• 2016

Rhizoctonia leaf blight (large patch) has become a serious problem in Korean lawn grass, which is extremely hard to treat and develops mostly from the roots of lawn grass to wither it away. Rhizoctonia leaf blight (large patch) is caused by Rhizoctonia solani AG2-2 (IV). To develop zoysia japonica with strong disease tolerance against this pathogenic bacterium, ${\beta}-1,3-glucanase$ was cloned from zoysia japonica, which is one of the PR-Proteins known to play a critical role in plant defense reaction. ${\beta}-1,3-glucanase$ is known to be generated within the cells when plant tissues have a hypersensitive reaction due to virus or bacterium infection and secreted outside the cells to play mainly the function of resistance against pathogenic bacteria in the space between the cells. This study utilized the commonly preserved part in the sequence of corn, wheat, barley, and rice which had been researched for their disease tolerance among the ${\beta}-1,3-glucanase$ monocotyledonous plants. Based on the part, degenerate PCR was performed to find out the sequence and full-length cDNA was cloned. E.coli over-expression was conducted in this study to mass purify target protein and implement in vitro activation measurement and antibacterial test. In addition, to interpret the functions of ZjGlu1 gene, each gene-incorporating plant transformation vectors were produced to make lawn grass transformant. Based on ZjGlu1 protein, antibacterial activity test was conducted on 9 strains. As a result, R. cerealis, F. culmorum, R.solani AG-1 (1B), and T. atroviride were found to have antibacterial activity. The gene-specific expression amount in each organ showed no huge difference in the organs based upon the transformant and against 18s gene expression amount.

• Journal of Environmental Science International
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• v.21 no.12
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• pp.1487-1494
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• 2012

This study has been carried out to verify curative effect of lavender and lawn, a green space composition factor. Thus, the physiological index value of blood pressure, heart rate and salivary amylase were measured with object of 16 women in their 20~30s for stress relaxation effect at lavender garden and lawn square, and impression assessment upon SD method as for psychological value was performed in this study. And a survey was conducted on necessity and reason of urban green space with object of 345 men and women urban workers. As a result of variance analysis between groups and multi test of Turkey upon object place, the blood pressure was lowered the same at lavender garden and lawn square, and especially, high blood pressure was greatly lowered to 30 mmHG at lavender garden. On the other hand, low blood pressure group showed the tendency of a little increasing at the same places. And for the pulse, there was difference between high blood pressure group and normal group at the same places. To conclude, at lavender garden and lawn square the blood pressure was made to be lowered when it's high, and when it's low, the effect of returning to sound status, the function of increasing, was verified. In case of impression assessment upon SD method, the lawn square was assessed as resting place and lavender garden diversional place. And over 90% of urban workers responded to the green space's necessity and curative effect, diversion and resting place were adopted for the reason in high-ranking items. As above the green space pattern that urban workers require and curative effect of lavender garden and lawn square were verified. Therefore, the functional green space plan is required, which curative effect, diversion and resting place are considered, for the future green space plan design.

• Asian Journal of Turfgrass Science
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• v.1 no.1
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• pp.18-29
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• 1987

This study was carried out to examine the effect of nitrogen application and clipping interval on the characteristics of several turf components of korean lawngrass for the basic data of lawn management. It was treated by Split plot design with three replications. The main plots were nitrogen levels with 0, 350, and 700kgN / ha, and the sub plots were clipping intervals with 10, 20, and 30 days The results obtained are summarized as follows ; 1. Increasing the rate of nitrogen fertilizer and frequent clipping increased tiller number of korean lawngrass and the maximum number of tillers obtained in October were recorded from 700kgN application and clipping treatment of 10 days interval. Meanwhile, treatment of 350kgN with 10 days clipping interval increased tillers much more than those of 700kgN with 20 and 30 days clipping intervals. 2. The average number of green leaves occurred during the growth period maximized by applying 700 kgN and clipping 10 days interval. 3. Increasing tiller numbers significantly decreased tops DM weight per tiller by clippng plants at interval of 10 and 20 days, irrespective of nitrogen applied, and with nil N, at the interval of 30 days. By applying 700kgN however, tops DM weight per tiller increased as the number of tillers increased consistently. 4. The highest tops DM weight was achieved from late August to early September by applying 350 and 700kgN. 5. During the growth period, nitrogen application increased unders(stolon+root) DM weight, and, at the same level of nitrogen applied, the increase in stolon DM weight enhanced by lengthening the clipping interval to 30 days. 6. Nitrogen efficiency to green leaves, stolon nodes and DM weight of root with high nitrogen was achieved as clipping interval was shortened.

• Korean Journal of Soil Science and Fertilizer
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• v.31 no.4
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• pp.368-372
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• 1998

A field assay was conducted to study whether the by-product of Autoclaved Lightweight Concrete(ALC) could be used as a silicate fertilizer for Korean Zoysiagrass. ALC application rates (0, 2.0, 4.0, 6.0 and $8.0Mg\;ha^{-1}$) were investigated for their effects on growth status, mineral nutrient concentrations, yield of fresh and dry matter of lawn grass and soil properties after experiments. The number of stolon and length of stolon at 180 day after Zoysiagrass transplanting were increased in the treatemnt of $2.0{\sim}6.0Mg\;ha^{-1}$ of ALC application, but those of $8.0Mg\;ha^{-1}$ of ALC plot were deceased with excess of ALC. The fresh weight and dry matter at harvesting time of lawn grass treated with $6.0Mg\;ha^{-1}$ of ALC was heavier than those of other ALC plots. There were statistically significant between the number of stolon and length of stolon remarkably. Mineral nutrient contents such as Si and Ca in Zoysiagrass were increased by higher ALC application, while T-N, available P and K were no difference with application lavel of ALC. Soil pH and Si contents in soil after experiment were increased by ALC application remarkably.

• Asian Journal of Turfgrass Science
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• v.1 no.1
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• pp.7-17
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• 1987

The experiment with two levels of nitrogen (0. and 300kg / ha / year) and two levels of clipping height (1.5cm and 4cm) was conducted on the field during the period 3 June to 23 October 1985. Clonal lines of korean lawngrass ( Zoysia japonica Steud.) and manilagrass ( Zoysia matrella ( L.) Merr.)of Daejon origin were established in June, as individual clone in rows 30cm apart with a 40cm spacing between clones, actually 4 clones each plot. The results obtained were as follows : 1. When no nitrogen was applied to korean lawngrass, leaf blade which appeared during the August / early September period remained green for a period of about 10 weeks and even leaves emerged in late September lived for 42 days. However, leaf longevity did not exceed 8 weeks as nitrogen was applied. In contrast the leaf longevity of manilagrass which emerged during the mid - August / early September period was 11 weeks and, under the nitrogen applied, 9 weeks, indicating that the life - saen of individual leaf of manilagrass may be longer than that of korean lawngrass. Meanwhile, clipping height had no effect on the leaf longevity in both grasses. 2. During the July / August period, tiller number, green leaf number and DM weight of korean lawngrass were increased significantly with fertilizer nitrogen, but were not with two levels of clipping height. This trend was reversed after late September : no effect of nitrogen was appeared. Instead, lax clipping increased tiller number, green leaf number and DM weight. Green leaves stimulated by lax clipping resulted in the occurrance of more dead leaves in late October. 3. The increase of tiller number, green leaf number, and DM weight of korean lawngrass due to nitrogen application appeared to be of significance in early September. Unlike korean lawngrass, however, this significant increase was maintained to late October when new green leaves still emerge. Clipping height had little effect on the growth of manilagrass by early September, but since then, lax clipping stimulated leaf appearance, possibly resulting in a remained green color of manilagrass turf. 4. Among the stolons outgrown until early September, the primary stolon was not influenced by nitrogen and clipping treatments to produce only 2 - 3 stolons. However, 1st branch stolon as affected by nitrogen increased significantly, so most of stolons which occurred consisted of 1st branch stolon. 5. Until early September, stolon length obtained at nil nitrogen level was chiefly caused by lengthening the primary stolons. By applying nitrogen the primary stolons of korean lawngrass was longer than 1st branch stolons when severe clipping was involved and in turn, shorter than 1st branch stolons when lax clipping was concerned. In manilagrass, 1st branch stolons were much longer than the primary stolons when turf was clipped severely but in conditions of lax clipping, there was little difference in length between primary and 1st branch stolons. 6. Stolon nodes of both korean lawngrass and manilagrass were positively influenced by nitrogen, but no particular increases by imposing clipping height treatment was marked in manilagrass. Although the stolon of korean lawngrass was grown until late october, the growth stimulated by nitrogen was not so remarkable as to exceed that a by nil N. 7. The thickness of korean lawngrass and manilagrass was greatest in late September, but that of manilagrass did not differ significantly from that in late October. 8. The response of stolon length of korean lawngrass to lax clippings was not so great in late October as to that to severe clippings. On the other hand, the positive effect of lax clippings to stolon length in m anilagrass was confirmed even in late October.