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Overcoming of Barriers to Transformation in Monocot Plants  

Toyama Koichi (College of Agriculture and Life Science, Sunchon National University)
Bae, Chang-Hyu (College of Agriculture and Life Science, Sunchon National University)
Seo, Mi-Suk (College of Agriculture, Osaka Prefecture University, Japan)
Song, In-Ja (College of Agriculture and Life Science, Sunchon National University)
Lim, Yong-Pyo (Kumho Life and Environmental Science Laboratory)
Song, Pill-Soon (College of Agriculture, Chungnam National University)
Lee, Hyo-Yeon (College of Agriculture and Life Science, Sunchon National University)
Publication Information
Journal of Plant Biotechnology / v.4, no.4, 2002 , pp. 135-141 More about this Journal
Abstract
Agrobacterium-mediated transformation has been unsuccessful for monocot plants except for a few important crops such as barley, rice, maize and wheat. We discussed here that a successful transformation of monocots demands certain critical conditions. The requirements for an efficient transformation are a selection of target tissues competent for plant regeneration and Agrobacterium-infection, and various factors promoting Agrobacterium-infection. The factors were divided into two to activate Agrobacterium and to increase plant cell's susceptibility against Agrobacterium. Optimization of these factors significantly increased transformation efficiency of zoysia grass and rice plants. A technical improvement in transformation system for monocots will promote improvement of the breed as well as a study of gene functions in monocots.
Keywords
Agrobacteriam; riu Zoysiagrass; transformation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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