• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2006.06a
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• pp.19.1-19.1
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• 2006

• Korean Journal of Agricultural Science
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• v.11 no.2
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• pp.201-206
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• 1984

In order to obtain an easy and effective scarification method for stimulating the seed germination of Korean lawn grass (Zoysia japonica Steud.), sodium hypochlorite (SH), which is used as a commercial bleach and a seed disinfestant, was tested as a chemical scarifier instead of potassium hydroxide (KOH). 1. Scarification with 4% SH for 2 hours followed by 8-hour water-rinse stimulated seed germination more effectively compared to generalized scarification method by KOH treatment. 2. The degree of stimulating seed gemination was different by the various water-rinsing time after SH treatment, but the optimum rinsing time was about 4 to 8 hours. 3. GA treatment after SH scarification stimulated seed germination. However, the stimulation effect of GA was not showed in non-scarified control. 4. SH treatment significantly decreased the percentage of the infection of microflora in seed samples compared to KOH treatment. 5. Under practical seed scarification conditions for stimulating germination of Korean lawn grass seed. SH may have advantages over KOH with respect to ease of preparing, securing and handling.

• Journal of Plant Biotechnology
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• v.43 no.2
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• pp.213-222
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• 2016

This study aims to establish a system for the rapid discrimination of Zoysia species using metabolite fingerprinting of FT-IR spectroscopy combined with multivariate analysis. Whole cell extracts from leaves of 19 identified Zoysia japonica, 6 identified Zoysia sinica, and 38 different unidentified Zoysia species were subjected to Fourier transform infrared spectroscopy (FT-IR). PCA (principle component analysis) and PLS-DA (partial least square discriminant analysis) from FT-IR spectral data successfully divided the 25 identified turf grasses into two groups, representing good agreement with species identification using molecular markers. PC (principal component) loading values show that the $1,100{\sim}950cm^{-1}$ region of the FT-IR spectra are important for the discrimination of Zoysia species. A dendrogram based on hierarchical clustering analysis (HCA) from the PCA and PLS-DA data of turf grasses showed that turf grass samples were divided into Zoysia japonica and Zoysia sinica in a species-dependent manner. PCA and PLS-DA from FT-IR spectral data of Zoysia species identified and unidentified by molecular markers successfully divided the 49 turf grasses into Z. japonica and Z. sinica. In particular, PLS-DA and the HCA dendrogram could mostly discriminate the 47 Z. japonica grasses into two groups depending on their origins (mountainous areas and island area). Considering these results, we suggest that FT-IR fingerprinting combined with multivariate analysis could be applied to discriminate between Zoysia species as well as their geographical origins of various Zoysia species.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2000.10a
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• pp.20.2-20
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• 2000

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.100-100
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• 2018

Zoysia japonica Steud.(Zj) is a typical warm-season Korean lawn grass, which is used in many places such as river banks, roadside and soccer fields in Korea. Recently, it has also been used in school yards and the Saemangeum reclaimed land to reduce water pollution. Although the cultivated area of turfgrass is steadily increasing worldwide, it grows fast requiring frequent mowing and is difficult to grow in shady areas and the cold region. Therefore this study aims searching for useful gene(s) to develop abiotic stress tolerant and dwarf zoysiagrass. We isolated Ycf4 gene based on the sequence from Oryza sativa Japonica through RT-PCR and RACE PCR. Ultimately, open reading frame (ORF) of ZjYcf4 was 558bp long, encoding a protein of 186 amino acid residues. NCBI blast results showed that the ZjYcf4 protein is evolutionarily closely related to Ycf4 protein from Zoysia macrantha and Setaria italica (100% and 98%, respectively). To determine whether ZjYcf4 was involved in environmental stress in wild-type zoysiagrass, expression patterns of the gene were analyzed by real-time PCR under salt, cold and dark conditions. They were analyzed after each stress treatment for 3 hours. In salt and cold stresses, the expression was higher compared to control (3-fold and 1.5-fold, respectively), although there was a 1.6-fold decrease in expression under dark stress treatment. As reported previously, we suggest that ZjYcf4 gene affects abiotic stress such as salt, cold and dark.

• Weed & Turfgrass Science
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• v.4 no.4
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• pp.338-347
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• 2015

Reinforcement cells are used to aid grass growth and taken together, this serves to extend greenery beyond the conventional spaces of lawns, tree pits, gardens, and parks, and is advantageous to urban cities since space for greening is often limited. Drought has variable effects on plant life and the resilience of turf to drought resistance also varies with species. Changes in photosynthetic ability were more pronounced for media rather than grass species. The media of sand without organic matter was found to be least suited for drought resistance. Normalized difference vegetation index (NDVI) and digital image analysis (DIA) data were generally in favour of Zoysia species as oppose to A. compressus. In A. compressus, selective traits such as, a more extensive root system and lower specific leaf area (SLA) were not an underlying factor that assisted this grass with enhanced drought resistance. Generally, WUE was found to be strongly related to plant characterises such as overall biomass, photosynthetic features as well as the lushness indexes, and specific leaf area. This study found a strong relationship between WUE and a suite of plant characteristics. These traits should serve as useful selection criteria for species with the ability to resist water stress.

• Weed & Turfgrass Science
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• v.6 no.2
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• pp.130-135
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• 2017

In the current study, we surveyed the status of graveyard lawn maintenance in Korea. The lawn maintenance information was obtained from the vicarious companies registered on the internet portal site and the Forestry Federation of Korea, and the questionnaire investigation from Primary Agricultural Cooperatives (Nonghyup). In all provinces surveyed, individual graves were maintained on a $66m^2$ (20 Pyeong) basis. The vicarious maintenance of graveyard lawn was carried out 1~5 times a year, and the maintenance cost increased as the number of times increased. Mowing the grass around a grave (Beolcho) was mainly done before the Korean Thanksgiving Day (Chuseok). The number of Beolcho was steadily increased from 2013 to 2015, and the price was getting higher and higher. Gyeonggi province had the highest cost, and Jeolla had the lowest cost. Regardless of the year, the number of orders received were higher in Gyeongnam > Gyeongbuk > Jeonnam province in order. Most of the reason for requested Beolcho was because the client lives in the area far from the graveyard. The direction of the cemetery was very varied. According to the survey, the all grass were zoysiagrass (Zoysia japonica), and grass coverage rate of the graves was 35.4~71.9%. Through questionnaire investigation, the reason why the density of grass was lowered was because the periodic management of weeds and fallen leaves was insufficient. Further research on the maintenance of graveyard in the future will be necessary.

• Journal of the Korean Institute of Landscape Architecture
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• v.34 no.5 s.118
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• pp.76-83
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• 2006

The purpose of this study was to investigate the performance of porous grass block. For the investigation, Festuca arundinacea and Zoysia japonica 'Zenith' were planted, and the volume of evapotranspiration and remains were examined based on different materials in the water tank in the experiment of Festuca arundinacea, the volume of water storage of treatment with perlite ($10.84{\iota}/m^2$) was higher than that with drainage ($7l/m^2$). The difference between the two was $3.84/m^2$. The drainage treatment without water storage capacity showed the higher degree of dryness in turf grass. The volume of evapo-transpiration of treatment with perlite was the highest (21.57mm/week). The volume of evapotranspiration of treatment with sand was 19.57mm/week, and with treatment with drainage was 18.24mm/week. Based on the measured volume of daily evapotranspiration of $2.60{\sim}3.08mm\;d^{-1}$, it was determined that the unit with water storage capacity would store water of one to two days usage compared to unite without such storage capacity. In the experiment of Zoysia japonica 'Zenith', the volume of water storage of treatment with perlite was $10.77l/m^2$ which was similar to the former experiment. The volume of evapotranspiration of treatment with perlite and sand were 21.64mm/week and 20.64mm/week, respectively. In case of airtight water tank, the volume was measured as 22.06mm/week. Each treatment has no notable difference in the volume of evapotranspiration. In conclusion, from the investigation in this study, porous grass block with water tank was found to be effective in plant growth under low irrigation. As the ecological area ratio and vegetated porous pavement have became more emphasized, additional study of rain infiltration and reservoir effect are needed in the future.

• Journal of the Korean Institute of Landscape Architecture
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• v.14 no.1
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• pp.1-13
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• 1986

The purpose of this study is to investigate the effect of temperature control by a shade tree and the lawn area. In this investigation, we find out that artificial-lawn, concerte, and exposed soil are more higher temperature than covered with plant materials. The results of the measurement may to summerized as follows; 1) Low-temperature effects of zoysia japonica is more controlled by condition of growth than leaf length of grass. Surface temperature make 0.7$^{\circ}C$ difference between long grass (15cm), and short grass (5cm), but make 5$^{\circ}C$ difference between good growth grass (230/10$\textrm{cm}^2$) and bad growth grass (80/10$\textrm{cm}^2$). 2) The surface temperature of the lawn area is 40.5$^{\circ}C$ lower on a maxinum than that of the artificial lawn (July 28, 1985). During the day of summer, shade area under the shade tree is 0.9$^{\circ}C$ lower then lawn area surface temperature, 6.9$^{\circ}C$ lower than bad growth lawn, 10.3$^{\circ}C$ lower than exposed soil, and 18$^{\circ}C$ lower than concrete surface temperature. 3) Natural irrigation effect on the surface temperature fluctuation. But this effect is changed by compositions of ground materials and time-lapse. 4) Sunny day is more effective than cloud day. 5) In summer season, surface temperature make a difference compare to temperature of 0.5-1.5m height from ground : Surface temperature is 3.4$^{\circ}C$ lower at the lawn area (11 a.m.), 4.2$^{\circ}C$ lower at the shade area the shade tree, 12.7$^{\circ}C$ higher at the concrete area (3p.m.), 38.8$^{\circ}C$ higher at the artificial lawn (2p.m.) 6) According to compositions of ground materials and season have specific vertical temperature distribution curve. 7) In summer season, temperature distribution of 0.5-1.5m hight at the shade tree is 4.8-5.7$^{\circ}C$ lower than concrete area (noon-3p.m.)

• Horticultural Science & Technology
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• v.35 no.3
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• pp.344-360
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• 2017

Zoysiagrasses are important turf plants used for school playgrounds, parks, golf courses, and sports fields. The two most popular zoysiagrass species are Zoysia japonica and Zoysia sinica. These are widely distributed across different growing zones and are morphologically distinguishable from each other; however, it is phenotypically difficult to differentiate those that grow along the coastal line from those in beach area habitats. A combination of morphological and molecular approaches is desirable to efficiently identify these two plant cultivars. In this study, we used a rapid identification system based on DNA barcoding of the nrDNA-internal transcribed spacer (ITS) regions. The nrDNA-ITS regions of ITS1, 5.8S nrDNA, and ITS2 from Z. japonica, Z. sinica, Agrostis stolonifera, and Poa pratensis were DNA barcoded to classify these grasses according to their molecular identities. The nrDNA-ITS sequences of these species were found at 686 bp, 687 bp, 683 bp, and 681 bp, respectively. The size of ITS1 ranged from 248 to 249 bp, while ITS2 ranged from 270 to 274 bp. The 5.8S coding region ranged from 163 - 164bp. Between Z. japonica and Z. sinica, nineteen (2.8%) nucleotide sites were variable, and the G+C content of the ITS region ranged from 55.4 to 63.3%. Substitutions and insert/deletion (indel) sites in the nrDNA-ITS sequence of Z. japonica and Z. sinica were converted to cleaved amplified polymorphic sequence (CAPS) markers, and applied to the Zoysia grasses sampled to verify the presence of these markers. Among the 62 control and collected grass samples, we classified three groups: 36 Z. japonica, 22 Z. sinica, and 4 Z. japonica/Z. sinica hybrids. Morphological classification revealed only two groups; Z. japonica and Z. sinica. Our results suggest that used of the nrDNA-ITS barcode region and CAPS markers can be used to distinguish between Z. japonica and Z. sinica at the species level.