• The Korean Journal of Ecology
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• 제24권2호
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• pp.81-85
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• 2001

Decomposition of Zizania latifolia was investigated with litterbag method for 13 months from November in 1998 to December in 1999, at the fringe of stream at Boryeong, Chungnam Province in Korea. After 13 months, remaining mass of leaves, culms and rhizomes was 16.9, 14.7, and 10.1%, respectively. Mass loss of the rhizomes was higher than those of the leaves and culms. The decay rate of leaves, culms and rhizomes was 1.86, 2.00 and 2.36 per year, respectively. Initial concentration of N, P, K, Ca, and Mg of leaves, culms and rhizomes was 18.0, 14.5, 44.0 mg/g for N,0.18, 0.12, 0.67 mg/g for P, 14.1 ,14.2, 14.8 mg/g for K,3.6, 1.5, 0.3 mg/g for Ca, 1.1, 0.5, 0.5 mg/g for Mg, respectively. Concentrations of N and P in rhizomes were higher than those in leaves and culms. Except for Mg in rhizomes, there was no immobilization period during the decomposition. Most of the N, P and Mg were lost during the first 3 months. In case of K and Ca, most were lost within 1 month.

• 한국식품과학회지
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• 제41권6호
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• pp.717-721
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• 2009

고장초 메탄올 추출물의 항알레르기 활성을 조사하기 위해, 비만세포주인 RBL-2H3 세포를 이용하였으며, 고장초 추출물을 처리한 후 IgE-항체, A23187 및 PMA를 이용하여 세포를 활성화시켜 분비되는 $\beta$-hexosaminidase, TNF-$\alpha$의 양을 측정하였다. 그 결과 고장초 추출물이 활성화된 비만세포에서 분비되는 $\beta$-hexosaminidase, TNF-$\alpha$를 농도 의존적으로 억제하는 것을 확인할 수 있었다. 또한, 비만세포에 탈과립이 발생할 경우 나타나는 형태학적 변화에 대한 시료의 영향을 검색하기 위해 고장초 추출물을 처리한 세포에 compound 48/80을 처리하였으며, 그 결과 고장초 추출물을 처리한 경우 대조군에 비해 탈과립이 현저히 억제되는 것을 관찰하였다. 따라서 고장초 추출물은 다양한 자극으로 인한 비만세포의 탈과립 저해를 통해 알레르기 반응을 억제시키는 것으로 판단되며, 본 연구결과는 고장초의 항알레르기 기능성 소재로의 이용 가능성을 시사해준다.

• 한국환경복원기술학회지
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• 제9권1호
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• pp.72-88
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• 2006

The optimal environmental ranges of the establishment phase for the distribution of Zizania latifolia and Typha angustifolia was determined to develop a set of basic data and criteria of planting substrate for the restoration, conservation and management of wetlands. The study was carried at 17 wetlands in the Kyunggi-do and Gyeongsangnam-do region where inland wetlands place intensively in June, 2005. Total 127 quadrats were sets in growing areas of Zizania latifolia and Typha angustifolia. $NO_3-N$, K, Ca, Mg and Na in the water variables and soil texture, LOI (loss on ignition), soil pH and soil conductivity in the soil variables were analyzed. The optimal range of water depth for the distribution of Zizania latifolia was -5~39cm, $NO_3-N$ content of water was <0.01~0.19ppm, K content of water was 0.1~5.9ppm, Ca content of water 0.5~44.9ppm, Mg content of water was 1.2~11.9ppm, Na content of water 3.4~29.9ppm, water conductivity was 48~450${\mu}S$/cm, respectively. The optimal range of LOI for the distribution of Zizania latifolia was 1.7~11.9%, soil conductivity was 25.5~149.9${\mu}S$/cm, respectively. The optimal range of water depth for the distribution of Typha angustifolia was -20~24cm, $NO_3-N$ content of water was <0.01~0.19ppm, K content of water was 0.2~2.9ppm, Ca content of water 0.6~19.9ppm, Mg content of water was 0.2~5.9ppm, Na content of water 3.5~19.9ppm, water conductivity was 96~450${\mu}S$/cm, respectively. The optimal range of LOI for the distribution of Typha angustifolia was 2.4~15.9%, soil conductivity was 17.6~149.9${\mu}S$/cm, respectively. The optimal soil texture were loam, silt loam and sandy loam in both species. The lower water depth (-20~40cm) is appropriate to increase biodiversity in both species dominated community and it is better to maintain water depth of 40~100cm for water purification. Both species appear frequently in the soil with high silt content.

• The Korean Journal of Ecology
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• 제28권2호
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• pp.93-98
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• 2005

정수식물인 줄(Zizania latifolia)의 생장에 따른 저토로부터의 무기영양소 흡수와 저토의 산화환원전위(Eh) 및 pH와 같은 물리, 화학적 특성과 $NH_4^+$와 $NO_3^-$의 질소형 그리고 유동성 인$(PO_4^{3-})$, 착화합물 인, 인회석 인 및 유기 인의 변화를 화분 재배실험을 통하여 조사하였다. 화분재배 실험에서 저토의 Eh는 식재 후에 식물구의 환원층이 무식물구의 것보다 높았다. 환원층의 $NH_4^+-N$ 함량은 식물구가 무식물구보다 적었고, $NO_3^--N$ 함량은 그것과 반대의 양상으로 나타났다. 형태별 인의 비율은 식재 전에 유기 인이 높았고, 식재 후에 착화합물 인이 높음으로써, 전자가 분해되어 후자로 변형됨이 확인되었다. 상층수와 침출수의 $NH_4^+-N$ 및 $PO_4^{3-}-P$ 농도는 무식물구보다 식물구에서 낮았다. 침출수의 $NO_3^--N$ 농도는 무식물구보다 식물구에서 높았고, 이는 뿌리로부터의 산소공급이 저토를 산화시켰기 때문인 것으로 판단되었다.

• Animal cells and systems
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• 제7권4호
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• pp.289-294
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• 2003

To quantify nutrient loading from emergent macrophytes through leaching in the littoral zones of Paldang Reservoir, we conducted incubation experiments using leaf litter of the emergent macrophyte, Zizaniz latifolia. To separate the leaching process from microbial decay, we used $HgCl_2$ to suppress microbial activity during the experiment. We measured electric conductivity, absorbance at 280nm, total nitrogen and dissolved inorganic nitrogen, total phosphorus and soluble reactive phosphorus, Na, K, Mg and Ca amounts in leaf litter and in water. In addition, we examined the effects of water temperature and ion concentrations of ambient water on the leaching process. A total of 6% of the initial ash-free dry mass of leaf litter was lost due to leaching during incubation (four days). Electric conductivity and A280 continued to increase and saturate during the incubation. To compare reaching rates of different nutrients, we fitted leaching dynamics with a hyperbolic saturation function [Y=AㆍX/(B+X)]. From these fittings, we found that ratios of leaching amounts to nutrient concentration in the litter were in the order of K > Na > Mg > P > Ca > N. Leaching from leaf litter of Z. latifolia was dependent on water temperature while it was not related with ion concentrations in the ambient water. Our results suggest that the leaching process of nutrients, especially phosphorus, from aquatic macrophytes provides considerable contribution to the eutrophication of the Paldang Reservoir ecosystem.

• Journal of Ecology and Environment
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• 제42권4호
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• pp.220-227
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• 2018

Background: To understand the ecological characteristics of floating mat in which endangered species of Iris laevigata and Menyanthes trifoliata inhabit, we surveyed the vegetation and water environments of a floating mat wetland and examined the relations between ecological characteristics of the mat. Results: Although Phragmites australis and Zizania latifolia were found at all experimental quadrats (n = 61) as the major vegetational components of the floating mat wetland, they showed relatively poor growth performances in terms of shoot height (< 2 m) and biomass production (<$300g/m^2$) compared with those in soil-based wetlands because of oligotrophic water condition. The competitiveness and distribution of P. australis and Z. latifolia seemed to be determined by water level difference by micro-topography rather than water chemistry. Conclusion: P. australis and endangered plant species mainly occurred in the area of relatively shallow water, whereas Z. latifolia and deep-water species such as Scirpus fluviatilis mostly inhabit in the area of deep water on the floating mat. Continuously maintained water level and oligotrophic water condition in the floating mat appeared to be important environments for endangered species such as I. laevigata and M. trifoliata.

• 한국환경과학회지
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• 제15권1호
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• pp.21-31
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• 2006

Aquatic plants grow in water with photosynthesis and purify water quality as taking organic and inorganic matter in water. Polluted water in stagnant stream channel where nutritive salts load is great can be purified by activities of aquatic plants. Aquatic plants should be fixed to bed easily to plant and sustainable environment is needed. So in this study, Mattress/Filter system is suggested to plant aquatic plant in stagnant stream channel. In the result of study, coverage of Phragmites australis, Zizania latifolia and Typha angustifolia which planted in mattress was $78\%,\;62\%\;and\;82\%$ and numbers of species in each mattress system were 7, 11, 3. The evenness index of each mattress system was 0.86, 0.91 and 0.79 and diversity index of each mattress system was 1.67. 2.18 and 0.87. Removal rates of phosphorus at Phragmites australis, Zizania latifolia and Typha angustifolia which planted in mattress were $68.7\%,\;62.7\%,\;55.3\%$ and removal rates of nitrogen of them were $79.8\%,\;74.7\%,\;64.9\%$. The removal rate of nitrogen was greater than phosphorus at all system and both removal rates were greater at Phragmites australis than at Zirania latifolia and at Typha angustifolia the rate was the least. Removal rates of $PO_4^{-3},\;NH_4-N,\;NO_{3-}N$ at Phragmites australis were $57.4\%,\;52.8\%,\;47.8\%$ and at Zizania latifolia were $82.6\%,\;77.2\%,\;67.5\%$ and at Typha angustifolia were $80.6\%,\;73.7\%,\;64.3\%$. It seems that removal effect is great by the planted mattress system.

• Journal of Ecology and Environment
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• 제30권3호
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• pp.231-236
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• 2007

This study examined the decomposition of blades and culms of aquatic emergent plant species, Zizania latifolia, Phragmites communis and Typha angustata, and changes in nutrient contents during decomposition. Z. latifolia, P. communis and T. angustata were the most frequently occurring species in Lake Paldang of Han River, Korea. Experiments were carried out from July 27 to December 14, 2005 in Lake Paldang using the litter bag method. The remaining masses of blade litter of each species at the end of experimental period were 21.2% of initial dry weight in Z. latifolia, 32.5% in P. communis, and 44.7% in T. angustata. In addition, the remaining mass of culm was 22.6% of initial dry mass in Z. latifolia, 56.4% in P. communis, and 38.1% in T. angustata. During the litter decomposition period, P, K, Na, and Mg concentration decreased rapidly within 10 days, but Ca and Mg concentration declined slowly. K contents remained below 10% of initial values in all litter samples retrieved during decomposition, whereas Ca and Mg concentration remained above 40% and 50% during decomposition in all three species. Na, P and Mn contents in litter varied among species and plant parts. P concentration in culms of P. communis remained at about 60% of initial concentration throughout the study, but the remaining P content in culms of Z. latifolia was only 10% of the original value at the end of the study period. The Mn concentration in blades of P. communis increased about 15-fold relative to the initial content by the end of experiment.

• 생명과학회지
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• 제31권3호
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• pp.338-345
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• 2021

고장초는 오랫동안 식용 및 의약 목적으로 차를 만드는 데 사용되어 왔다. 그러나 고장초를 고부가가치 식용 재료로 사용하는 연구는 불충분하다. 이전 연구에서 우리는 tricin이 고장초의 주요 화합물임을 확인했다. 본 연구에서는 고장초 추출물의 HepG2 세포 보호 효과를 조사하였다. 고장초 추출물 또는 tricin의 HepG2 세포에 대한 독성 실험 결과, 사용한 모든 농도에서 고장초 추출물 또는 tricin에 대한 독성은 나타나지 않았다. 또한 tert-butyl hydroperoxide (t-BHP)에 의해 감소된 세포 생존율은 고장초 추출물(100 ㎍/ml에서 69.23%) 또는 tricin (100 ng/ml에서 74.47%)에 의해 증가되었다. 또한 고장초 추출물(250 ㎍/ml) 또는 tricin (250 ng/ml)은 산화적 손상에 의한 활성 산소종(ROS) 생성을 효과적으로 억제했다. 또한 항산화 효소로 알려진 superoxide dismutase 1(SOD1), catalase, heme oxygenase-1 (HO-1), nuclear factor erythroid-related factor 2 (Nrf2)의 단백질 발현은 고장초 추출물(100 ㎍/ml에서 2.1, 1.6, 1.7, 1.5배) 또는 tricin (250 ng/ml에서 2.6, 1.8, 2.2, 2.0배) 처리로 증가했으며, 이는 고장초 추출물의 HepG2 세포 보호 효과는 tricin의 활성과 관련이 있다는 것을 시사한다. 종합하면 고장초는 간 기능개선 관련 기능성 식품 소재로 개발이 가능할 것으로 기대된다.

• 생명과학회지
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• 제27권9호
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• pp.994-1002
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• 2017

본 연구에서는 고장초 줄기 ethanol 추출물 5종(10, 30, 50, 70, 90% Z. latifolia ethanol extract)에 대한 다양한 효능을 평가하여 원료제조를 위한 추출 조건을 최적화하고자 하였다. Z. latifolia ethanol extracts의 항산화능 평가결과, ethanol의 함량이 증가함에 따라 DPPH 및 ABTS 라디칼에 대한 $RC_{50}$ 값은 감소하여 소거능이 증가함을 확인하였다. Z. latifolia ethanol extracts의 항염증 평가 결과, ethanol의 함량이 증가함에 따라 lipopolysaccharide (LPS)로 처리한 RAW 264.7에서 nitric oxide (NO)의 생성 억제능이 농도의존적으로 증가되는 경향을 확인하였으며, $250{\mu}g/ml$의 농도에서의 NO 생성능 비교 시, 15.69, 14.13, 11.86, 8.64, $10.09{\mu}M$로 70% Z. latifolia ethanol extract에서 가장 높은 NO 억제 활성을 나타냈다. Z. latifolia ethanol extracts의 항알러지 효능을 평가하기 위하여 RBL-2H3 세포를 IgE-항원으로 활성화 시킨 결과, 10% Z. latifolia ethanol extract의 ${\beta}-hexosaminidase$ 방출 억제효과는 나타나지 않았으나, 30% Z. latifolia ethanol extract부터 농도의존적으로 ${\beta}-hexosaminidase$의 방출 억제효과를 확인할 수 있었다. 특히, $100{\mu}g/ml$ 추출물 처리 시 ${\beta}-hexosaminidase$ 방출은 각각 94.20, 57.10, 46.70, 36.38, 27.45%로 90% Z. latifolia ethanol extract의 항알러지 활성이 가장 우수하였다. Z. latifolia ethanol extracts의 미백 효능 평가 결과, ethanol 함량이 증가함에 따라 멜라닌 생성 억제 효과도 증가함을 확인할 수 있었으며, 특히 $500{\mu}g/ml$ 농도의 70%, 90% Z. latifolia ethanol extracts의 멜라닌 생성 억제능은 양성대조군인 arbutin ($250{\mu}M$)과 비슷한 효과를 나타내었다. 본 연구 결과, Z. latifolia ethanol extracts는 세포 독성이 없으며 피부 보호 효능과 관련된 다양한 활성이 우수함을 확인하였다. 이러한 원료 제조 조건에 대한 활성 결과는 향후 피부건강을 위한 기능성 식품 원료 제조를 위한 기초 결과로 활용될 것으로 기대된다.